##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8446332
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13714071386254	31.0	31.0	33.0	30.0	34.0
2	31.22756990845257	31.0	31.0	34.0	28.0	34.0
3	31.293109600711883	31.0	31.0	34.0	28.0	34.0
4	35.10573950917392	35.0	35.0	37.0	32.0	37.0
5	34.94992583762987	35.0	35.0	37.0	32.0	37.0
6	35.0325138770297	36.0	35.0	37.0	32.0	37.0
7	34.831810305349116	35.0	35.0	37.0	32.0	37.0
8	34.80335902022322	35.0	35.0	37.0	32.0	37.0
9	36.41564125113718	38.0	35.0	39.0	32.0	39.0
10	36.20877168929661	38.0	35.0	39.0	32.0	39.0
11	36.32780122779924	38.0	35.0	39.0	32.0	39.0
12	36.22512967759259	38.0	35.0	39.0	32.0	39.0
13	36.31707527006989	38.0	35.0	39.0	32.0	39.0
14	37.26094463253398	39.0	36.0	41.0	32.0	41.0
15	37.159318861726014	39.0	36.0	41.0	31.0	41.0
16	37.0761323376822	39.0	36.0	40.0	31.0	41.0
17	37.06563772297845	39.0	36.0	40.0	31.0	41.0
18	37.061843531606385	39.0	36.0	40.0	31.0	41.0
19	37.0606811335382	39.0	36.0	40.0	31.0	41.0
20	37.00668574240274	39.0	36.0	40.0	31.0	41.0
21	36.8643740265005	39.0	36.0	40.0	31.0	41.0
22	36.85066180206982	39.0	35.0	40.0	31.0	41.0
23	36.62283272786341	38.0	35.0	40.0	30.0	41.0
24	36.518084299788356	38.0	35.0	40.0	30.0	41.0
25	36.41867949306279	38.0	35.0	40.0	30.0	41.0
26	36.0616242648288	38.0	35.0	40.0	29.0	41.0
27	36.17096699490382	38.0	35.0	40.0	30.0	41.0
28	36.01301144686238	38.0	35.0	40.0	29.0	41.0
29	35.903523920205835	38.0	34.0	40.0	29.0	41.0
30	35.94761193379564	38.0	35.0	40.0	29.0	41.0
31	35.835746451832584	38.0	34.0	40.0	29.0	41.0
32	35.552479585221135	38.0	34.0	40.0	27.0	41.0
33	35.47450230466906	38.0	34.0	40.0	27.0	41.0
34	35.43246831879211	38.0	34.0	40.0	27.0	41.0
35	35.128474466786294	38.0	34.0	40.0	25.0	41.0
36	34.96678499021824	38.0	33.0	40.0	25.0	41.0
37	34.70438126277774	38.0	33.0	40.0	24.0	41.0
38	34.53972742250719	38.0	33.0	40.0	24.0	41.0
39	34.436268429893595	38.0	33.0	40.0	23.0	41.0
40	34.254082955772994	38.0	33.0	40.0	22.0	41.0
41	34.05868346164939	38.0	33.0	40.0	21.0	41.0
42	33.85423921295067	37.0	33.0	40.0	20.0	41.0
43	33.38127485398395	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	7.0
13	17.0
14	39.0
15	115.0
16	338.0
17	752.0
18	1599.0
19	3211.0
20	6332.0
21	11403.0
22	19343.0
23	30860.0
24	48014.0
25	71104.0
26	100868.0
27	140387.0
28	187172.0
29	241329.0
30	299560.0
31	360280.0
32	424199.0
33	502672.0
34	597850.0
35	708097.0
36	863949.0
37	1101252.0
38	1393819.0
39	1331761.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.441425224582694	19.12084440914707	12.498040569563214	24.93968979670702
2	16.043413874803882	25.969095223820233	32.26965267289991	25.71783822847598
3	22.21570262689177	27.131078910940275	28.99729728833771	21.65592117383025
4	16.2886564250612	17.959642126309976	31.006465291679277	34.74523615694955
5	13.892148686554115	37.91063387041854	33.11573591945	15.081481523577336
6	33.19649286814679	31.386630314792267	17.53994515015512	17.876931666905822
7	24.869671237171353	31.395166564610534	23.77530269944397	19.959859498774144
8	27.71471687354937	31.48753802242204	23.6433637702141	17.154381333814488
9	27.783752758001935	14.215413270517901	20.998251075141255	37.00258289633891
10	18.483313229932236	27.423371470598124	32.78543869693969	21.30787660252995
11	32.34792333524185	23.010580213991112	20.849085733310034	23.792410717456995
12	21.302690919561297	28.312514828922186	29.551312924947776	20.83348132656874
13	30.794148276435262	18.989757920953142	25.838304722097117	24.377789080514475
14	22.519065080558047	22.67225583839233	26.977734240141167	27.830944840908455
15	28.172489549309688	24.407423245972335	24.647231484625514	22.772855720092462
16	21.911156227342236	25.427345266560682	30.197984166381335	22.463514339715747
17	24.278846723050908	27.50902995525158	27.605699136619304	20.60642418507821
18	24.470716992891113	22.076695540738868	30.908695040640126	22.54389242572989
19	23.315647549729277	28.97690974022807	28.669427154888062	19.03801555515459
20	22.762223886060838	24.392316096502007	30.172079430455728	22.673380586981427
21	21.744089623756206	27.74752401397435	28.989293814166906	21.519092548102535
22	22.880204093327137	27.44462329920254	25.32011528791433	24.355057319555993
23	19.88729545558948	26.417254259008526	28.26941919877173	25.426031086630267
24	21.014021234306206	30.743889773691112	27.087663615401336	21.154425376601345
25	23.1243337344542	26.089916901206344	24.84204977971503	25.943699584624426
26	22.26235009469199	26.706504077746413	28.141351772580098	22.8897940549815
27	24.024345715986538	25.960949676143443	25.93429905431139	24.080405553558634
28	21.42673292974986	27.206685695044904	28.96714218669122	22.39943918851402
29	22.62138168378889	25.2714669515714	29.28934121936007	22.817810145279633
30	21.2881047062796	28.51827278397297	29.911362707504274	20.282259802243154
31	24.849887501462174	25.94590172396728	25.15482460315318	24.049386171417368
32	18.814782558866973	27.86112362147261	28.585615625812483	24.738478193847932
33	21.360905538640914	26.879608805337043	29.10649261715026	22.652993038871784
34	20.64715192346216	26.683476330317113	28.798856118845436	23.87051562737529
35	20.594643923539827	28.49713934995688	30.167402844216873	20.740813882286417
36	22.88746168159149	26.73926386033606	27.46890602926809	22.90436842880436
37	20.99010552746447	27.259986938709012	29.003915545825098	22.745991988001418
38	22.16959977419784	26.393255675954958	29.153601823844955	22.283542726002246
39	22.2562054155579	23.5783177833881	28.83604385903846	25.32943294201554
40	21.631981788070846	26.020857337836116	29.50918813042158	22.837972743671454
41	20.037999927068935	25.62410523289873	29.82743278384037	24.51046205619197
42	22.051797158814026	26.73340332821395	29.19155912886209	22.02324038410993
43	20.95414909099003	23.977710087645146	30.11049056560883	24.95765025575599
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3590.0
1	4076.5
2	4563.0
3	14548.5
4	24534.0
5	24534.0
6	35333.0
7	46132.0
8	46236.0
9	46340.0
10	55896.0
11	65452.0
12	65452.0
13	94694.5
14	123937.0
15	157805.5
16	191674.0
17	171474.0
18	151274.0
19	151274.0
20	148334.5
21	145395.0
22	119614.5
23	93834.0
24	93444.5
25	93055.0
26	93055.0
27	95098.5
28	97142.0
29	99480.5
30	101819.0
31	110175.5
32	118532.0
33	118532.0
34	139583.0
35	160634.0
36	194570.0
37	228506.0
38	261680.0
39	294854.0
40	294854.0
41	320419.0
42	345984.0
43	391111.0
44	436238.0
45	530019.5
46	623801.0
47	623801.0
48	979744.5
49	1335688.0
50	1314362.0
51	1293036.0
52	920082.5
53	547129.0
54	547129.0
55	482810.0
56	418491.0
57	403539.5
58	388588.0
59	361053.0
60	333518.0
61	333518.0
62	276288.5
63	219059.0
64	191290.0
65	163521.0
66	139987.0
67	116453.0
68	116453.0
69	99346.0
70	82239.0
71	72076.0
72	61913.0
73	46784.5
74	31656.0
75	31656.0
76	25092.5
77	18529.0
78	16410.5
79	14292.0
80	12069.0
81	9846.0
82	9846.0
83	8315.5
84	6785.0
85	5927.0
86	5069.0
87	3447.0
88	1825.0
89	1825.0
90	1298.0
91	771.0
92	533.5
93	296.0
94	303.5
95	311.0
96	311.0
97	162.0
98	13.0
99	13.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8446332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.94318262010105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62652284255859	27.367207888687144
2	9.718747553581588	6.597704460997604
3	3.399581380691847	3.461778349101559
4	1.682998298365836	2.285052743630035
5	0.9723759032024302	1.6502766428892897
6	0.6273380538074149	1.2776310074954318
7	0.45160635469729493	1.073026987892189
8	0.34821670256782644	0.9455666501303315
9	0.2729704521416454	0.8338937316241882
>10	1.6237768961309862	10.163847817883447
>50	0.12727251629069072	2.9965892013716426
>100	0.10865373071728081	7.857878842856845
>500	0.020129448310361092	4.7941988085950955
>1k	0.016863237983549745	11.059853978328624
>5k	0.0013135574850035353	3.1822703765553517
>10k+	0.0016330714678422329	14.453222511961265
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	92756	1.098180843471462	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	88803	1.051379462706415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	64393	0.7623782726040131	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	60214	0.7129011741428114	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	50807	0.6015273849050689	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	41901	0.496085164542431	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	41062	0.48615185858192644	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	38914	0.46072070100962165	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	37565	0.44474927104451967	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37393	0.44271288412532206	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	35633	0.42187543658004445	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34658	0.4103319642183139	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	28876	0.34187621324854384	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	28120	0.3329255823711405	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	26294	0.31130673054291497	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	25800	0.30545803787963816	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	25607	0.30317302232495713	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	25434	0.30112479594692704	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	24593	0.2911678110687574	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	20254	0.23979639919434853	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	20087	0.23781920956931366	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	18824	0.22286597306381042	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	18586	0.22004818186166492	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	17810	0.2108607618076107	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	17753	0.2101859126541557	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	17291	0.2047160826735203	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	16466	0.19494852913667138	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	16294	0.1929121422174738	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16236	0.19222545360518625	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	15001	0.17760372194699428	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	14991	0.17748532735866884	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	14498	0.17164847415422457	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	14383	0.17028693638848202	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	14355	0.16995543154117077	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	14141	0.16742178735100632	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	12881	0.15250406922200074	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	12842	0.1520423303275315	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	11925	0.14118554657808857	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11580	0.13710093328086084	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	11120	0.13165478221789056	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10921	0.12929872991021427	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	10761	0.1274044164970072	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	10721	0.12693083814370545	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	10397	0.12309485348196117	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	10262	0.12149652653956772	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	10199	0.12075064063311744	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	9825	0.11632268302974594	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	9506	0.11254589566216436	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	9409	0.11139746815540758	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	9295	0.11004776984849754	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	9198	0.10889934234174076	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	9074	0.10743124944650528	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8978	0.10629466139858107	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	8760	0.10371365937308645	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	8720	0.10324008101978469	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	8650	0.10241131890150659	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1839458832544116E-5
2	0.0	0.0	0.0	0.0	1.1839458832544116E-5
3	0.0	0.0	0.0	0.0	1.1839458832544116E-5
4	0.0	0.0	0.0	1.1839458832544116E-5	1.1839458832544116E-5
5	0.0	0.0	0.0	1.1839458832544116E-5	1.1839458832544116E-5
6	0.0	0.0	0.0	1.1839458832544116E-5	2.367891766508823E-5
7	0.0	0.0	0.0	1.1839458832544116E-5	4.735783533017646E-5
8	0.0	0.0	0.0	1.1839458832544116E-5	4.735783533017646E-5
9	0.0	0.0	0.0	3.551837649763235E-5	8.28762118278088E-5
10	0.0	0.0	0.0	1.8943134132070585E-4	8.28762118278088E-5
11	0.0	0.0	0.0	2.6046809431597053E-4	8.28762118278088E-5
12	0.0	0.0	0.0	6.038124004597499E-4	9.471567066035293E-5
13	0.0	0.0	0.0	7.932437417804558E-4	9.471567066035293E-5
14	0.0	0.0	0.0	0.0012549826362496762	1.5391296482307349E-4
15	1.1839458832544116E-5	0.0	0.0	0.0020482263780301317	2.0127080015324995E-4
16	1.1839458832544116E-5	0.0	0.0	0.0049015359566732634	2.367891766508823E-4
17	1.1839458832544116E-5	0.0	0.0	0.00822842388861816	2.367891766508823E-4
18	1.1839458832544116E-5	0.0	0.0	0.01050159998446663	2.367891766508823E-4
19	1.1839458832544116E-5	0.0	0.0	0.01601878780043219	2.6046809431597053E-4
20	1.1839458832544116E-5	0.0	0.0	0.020790089709947467	2.6046809431597053E-4
21	1.1839458832544116E-5	0.0	0.0	0.03141008428273954	2.7230755314851463E-4
22	1.1839458832544116E-5	0.0	0.0	0.046742183470884165	2.7230755314851463E-4
23	1.1839458832544116E-5	0.0	0.0	0.06562612030879203	3.315048473112352E-4
24	1.1839458832544116E-5	0.0	0.0	0.09518924901365468	3.315048473112352E-4
25	1.1839458832544116E-5	0.0	0.0	0.11442836961653888	3.433443061437793E-4
26	1.1839458832544116E-5	0.0	0.0	0.15186473844504336	3.433443061437793E-4
27	1.1839458832544116E-5	0.0	0.0	0.2350961340378285	3.433443061437793E-4
28	1.1839458832544116E-5	0.0	0.0	0.38467585692819084	3.433443061437793E-4
29	1.1839458832544116E-5	0.0	0.0	0.5119618788368726	3.433443061437793E-4
30	1.1839458832544116E-5	0.0	0.0	0.7293698613788802	3.433443061437793E-4
31	1.1839458832544116E-5	0.0	0.0	1.170792244491455	3.433443061437793E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	4860	0.0	16.558643	2
TATCACG	395	0.0	15.92405	2
AAAAAGT	5120	0.0	15.681641	1
TGGGCTG	7455	0.0	14.41784	36
ACCGTCG	1970	0.0	14.180203	8
GGGCTGT	7940	0.0	13.909949	37
AGTACTC	5880	0.0	13.875	5
AAAGTAC	5930	0.0	13.820405	3
TACCGTC	2025	0.0	13.795062	7
GGTTATC	1590	0.0	13.729559	2
AGGTTAT	1545	0.0	13.650485	1
AAGTACT	6080	0.0	13.631579	4
ATACCGT	2090	0.0	13.631578	6
CAAGACG	2205	0.0	13.5079365	4
AAGACGG	2170	0.0	13.470046	5
CGAGCCG	2165	0.0	13.415704	15
CACTCTA	2255	0.0	13.126386	9
ACGAACG	1170	0.0	13.123932	15
CCGTCGT	2060	0.0	13.021845	9
CTTATAC	10440	0.0	12.988985	37
>>END_MODULE