FastQCFastQC Report
Thu 9 Feb 2017
SRR2727186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4102958
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC391600.9544333624667861No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG387320.9440018640210306No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA254910.6212834740204506No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG215880.5261569823527318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213870.521258077708814No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG180900.44090141795260884No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC165570.40353813029526503No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT157520.38391813905967354No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG144470.3521118178640873No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT143130.34884588143480877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138730.3381219110700134No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC133240.3247413207739392No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT124570.30361022462330833No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG123460.3009048593721895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118530.28888913803163474No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC108850.26529640322908493No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA107640.2623473113787662No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT106850.2604218712450871No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105500.2571315621558885No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT89480.21808656096406545No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT87800.2139919540975072No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC85080.2073625905992701No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC80090.19520063329919535No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT79340.19337268380519615No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC78080.1903017286552775No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT76560.18659708434743907No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT71400.17402079182872454No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC69850.1702430295411262No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA69120.16846382536696694No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT64870.15810544490097145No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG63430.15459578187249295No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG61330.1494775232892952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC59790.1457241336616168No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT55770.13592632437378105No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT53770.13105179238978318No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.12627475104546526No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT51720.12605539710618535No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG50760.12371562175386636No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50560.12322816855546657No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC48560.11835363657146869No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG44930.10950636102051252No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT44410.10823898270467305No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT43650.10638666055075388No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC43540.106118561291634No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT43150.10516802755475439No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG41730.10170710984611589No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG41330.10073220344931633No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT41210.10043973153027645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA19250.016.6259752
GCGCTAG904.4504377E-516.44444526
AAAAAGT19900.016.0829141
ACGAACG8800.015.34659115
TGGGCTG35650.015.15287636
AAGACGG13800.015.1485515
TATACTG3750.014.7999995
AACGAAC9350.014.6417114
CGAACGA9200.014.47826116
CGCTAGG1152.2124716E-514.47826127
CAAGACG14050.014.3523134
ATAACGA9550.014.33507812
TAACGAA9450.014.29100413
TCGTTAC3650.014.19178123
GGGCTGT40850.014.17503137
ACCGTCG9950.013.9447238
ATAAGAC2800.013.8750013
GTTACGG4300.013.76744225
GTAATAC1753.5732228E-813.7428583
AGTACTC24250.013.7319595
Produced by FastQC (version 0.11.5)