##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727186.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4102958
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205394010857532	31.0	31.0	33.0	30.0	34.0
2	31.315226721794374	31.0	31.0	34.0	30.0	34.0
3	31.378084055454625	31.0	31.0	34.0	28.0	34.0
4	35.19554331289767	35.0	35.0	37.0	33.0	37.0
5	35.05316578916967	35.0	35.0	37.0	32.0	37.0
6	35.110077899895636	36.0	35.0	37.0	32.0	37.0
7	34.915051287388266	36.0	35.0	37.0	32.0	37.0
8	34.85287492584618	36.0	35.0	37.0	32.0	37.0
9	36.48415387142642	38.0	35.0	39.0	32.0	39.0
10	36.26710071124296	38.0	35.0	39.0	32.0	39.0
11	36.388750018888814	38.0	35.0	39.0	32.0	39.0
12	36.262058495358716	38.0	35.0	39.0	32.0	39.0
13	36.353947079156065	38.0	35.0	39.0	32.0	39.0
14	37.326637269989114	39.0	36.0	41.0	32.0	41.0
15	37.19789795557254	39.0	36.0	41.0	31.0	41.0
16	37.13199574550848	39.0	36.0	40.0	31.0	41.0
17	37.08170641766257	39.0	36.0	40.0	31.0	41.0
18	37.067221258418925	39.0	36.0	40.0	31.0	41.0
19	37.07717773372284	39.0	36.0	40.0	31.0	41.0
20	36.990954087270694	39.0	36.0	40.0	31.0	41.0
21	36.86532594289291	39.0	36.0	40.0	31.0	41.0
22	36.84582732750372	39.0	35.0	40.0	31.0	41.0
23	36.62962672296426	39.0	35.0	40.0	30.0	41.0
24	36.51185924886387	38.0	35.0	40.0	30.0	41.0
25	36.409277891706424	38.0	35.0	40.0	30.0	41.0
26	36.05432495287546	38.0	34.0	40.0	29.0	41.0
27	36.19518771578944	38.0	35.0	40.0	30.0	41.0
28	36.04950355329009	38.0	35.0	40.0	29.0	41.0
29	35.95825060846345	38.0	34.0	40.0	29.0	41.0
30	36.05263519636321	38.0	35.0	40.0	29.0	41.0
31	35.97716476746776	38.0	35.0	40.0	29.0	41.0
32	35.74374560987463	38.0	34.0	40.0	27.0	41.0
33	35.69693840395149	38.0	34.0	40.0	27.0	41.0
34	35.69179894115416	38.0	34.0	40.0	27.0	41.0
35	35.40567463766385	38.0	34.0	40.0	26.0	41.0
36	35.276150523597856	38.0	34.0	40.0	26.0	41.0
37	35.04184712590283	38.0	33.0	40.0	25.0	41.0
38	34.900698715414585	38.0	33.0	40.0	24.0	41.0
39	34.84067860309562	38.0	33.0	40.0	24.0	41.0
40	34.696172858703406	38.0	33.0	40.0	24.0	41.0
41	34.53894848545854	38.0	33.0	40.0	23.0	41.0
42	34.368009860203294	38.0	33.0	40.0	23.0	41.0
43	33.90980580352029	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	11.0
14	14.0
15	59.0
16	160.0
17	329.0
18	653.0
19	1291.0
20	2464.0
21	4648.0
22	8142.0
23	13436.0
24	20806.0
25	31607.0
26	44950.0
27	63598.0
28	85151.0
29	111327.0
30	139712.0
31	169176.0
32	201887.0
33	240399.0
34	288779.0
35	344006.0
36	419121.0
37	529930.0
38	692086.0
39	689212.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.04433533075406	19.009894812474318	12.309119420671623	25.636650436100005
2	16.36616801829314	25.115099886472148	32.52465660140806	25.994075493826653
3	21.390372506859684	26.43339268888446	29.077875035523153	23.098359768732703
4	15.713711912235025	17.605542147884528	31.889919419111774	34.790826520768675
5	13.860268615959509	37.721370776888286	33.546163523974656	14.872197083177552
6	33.69873637507379	32.1704974801107	17.097591542492026	17.033174602323495
7	25.76443629206051	31.140387008592334	23.31764059003285	19.777536109314305
8	27.77006247687644	31.581556525804068	23.089853710420627	17.55852728689887
9	27.97440285764563	13.995707487134892	20.142346082996706	37.88754357222277
10	18.211324610195863	26.765007099755834	32.89668088242678	22.126987407621527
11	33.81638320450758	22.60634888292788	20.443299687688736	23.133968224875808
12	21.625129967209023	27.442737654150978	29.160010899453514	21.77212147918648
13	31.0880345350842	18.808332914936006	25.03106295506803	25.07256959491177
14	22.943763986860212	22.151408812861355	25.684372104223343	29.220455096055094
15	28.11459439750541	24.802983603536767	23.5732610472737	23.509160951684127
16	23.166261999269793	25.22536179995018	28.609773729099835	22.998602471680186
17	24.962502662713096	26.993866376404537	26.64221763907893	21.40141332180344
18	25.51517709905878	21.95350281431104	29.445414747116594	23.08590533951359
19	24.09880871312843	28.559834148923773	27.115437204085442	20.22591993386235
20	23.57279796673522	24.126910390016178	28.633829544440864	23.666462098807738
21	22.8380353881273	27.36023620032182	27.277954100431934	22.523774311118956
22	23.688811827954368	26.862424621456032	24.135489566308017	25.313273984281587
23	20.82302572924217	25.798289916689377	26.775804188100388	26.602880165968063
24	21.90132094942234	30.038767152868733	25.69124519432078	22.36866670338814
25	24.12203585803218	25.361678086882684	23.659515890730543	26.856770164354593
26	23.145325884398524	26.148573785059465	26.772879468909995	23.933220861632023
27	25.222217726820507	25.269013233866882	24.65250680119075	24.85626223812186
28	22.574371953112852	26.290934491652116	27.544444763997095	23.59024879123793
29	23.52368705699644	24.92899025532311	27.83360200128785	23.7137206863926
30	22.450900057958183	27.70415880445279	28.448841055648145	21.396100081940883
31	25.589343103195304	25.405816973997787	23.951281002632737	25.05355892017418
32	20.137349687713108	27.020554439016927	27.03366693005388	25.80842894321609
33	22.3231385746576	26.096221311551325	27.616319738101147	23.96432037568993
34	21.903953196693703	26.033851674816074	27.5357924697255	24.526402658764727
35	22.035394951642207	27.506009079303272	28.73185638263906	21.72673958641546
36	23.805288769712	26.038994306059188	26.41740422397695	23.738312700251868
37	22.289991757166415	26.332733603414905	27.83776972613417	23.53950491328451
38	23.11044860805302	25.441644784080168	28.143500372170514	23.304406235696295
39	23.348764476750674	22.799794684712836	27.735843262348777	26.115597576187717
40	22.706959223077593	25.030502383889868	28.495100364176285	23.767438028856255
41	21.09833929569837	24.67873178326466	29.15330841797552	25.06962050306145
42	23.188806709695786	25.615689948568814	28.54433313721466	22.65117020452074
43	21.872951173275474	23.010642565680662	29.47585619935666	25.6405500616872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1319.0
1	1408.5
2	1498.0
3	4894.5
4	8291.0
5	8291.0
6	11597.0
7	14903.0
8	14779.0
9	14655.0
10	17397.0
11	20139.0
12	20139.0
13	28882.5
14	37626.0
15	49132.5
16	60639.0
17	54918.5
18	49198.0
19	49198.0
20	48988.0
21	48778.0
22	41523.5
23	34269.0
24	36115.0
25	37961.0
26	37961.0
27	40601.5
28	43242.0
29	45985.0
30	48728.0
31	53505.5
32	58283.0
33	58283.0
34	70211.0
35	82139.0
36	100869.5
37	119600.0
38	137956.5
39	156313.0
40	156313.0
41	169506.5
42	182700.0
43	203523.5
44	224347.0
45	269115.5
46	313884.0
47	313884.0
48	473940.0
49	633996.0
50	620989.0
51	607982.0
52	444708.5
53	281435.0
54	281435.0
55	253416.5
56	225398.0
57	218195.5
58	210993.0
59	196296.5
60	181600.0
61	181600.0
62	150871.5
63	120143.0
64	105771.0
65	91399.0
66	78135.5
67	64872.0
68	64872.0
69	54848.0
70	44824.0
71	39277.5
72	33731.0
73	25417.5
74	17104.0
75	17104.0
76	13413.0
77	9722.0
78	8673.5
79	7625.0
80	6492.0
81	5359.0
82	5359.0
83	4501.0
84	3643.0
85	3203.5
86	2764.0
87	1893.5
88	1023.0
89	1023.0
90	725.0
91	427.0
92	322.0
93	217.0
94	193.0
95	169.0
96	169.0
97	89.5
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4102958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.613261538499074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8419015806502	31.987960120802462
2	8.689307163412867	6.710449801784291
3	2.9602841307853103	3.429186761108448
4	1.474784218822189	2.2778491501692892
5	0.8664118417791815	1.6727493523336108
6	0.56485781979336	1.3086601636648436
7	0.41280259841364275	1.1157758287422592
8	0.30219693891710847	0.9335047550832112
9	0.24775179002511424	0.8609854198383938
>10	1.3748788475515978	9.646555475349185
>50	0.11029071558283547	2.9904421453403227
>100	0.11756045284586067	9.713254284703861
>500	0.020353869075085105	5.536026086429726
>1k	0.014106004200451749	10.193925440638239
>5k	0.0012882195616850914	3.35912636461416
>10k+	0.0012238085836008368	8.263548849397779
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	39160	0.9544333624667861	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	38732	0.9440018640210306	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	25491	0.6212834740204506	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	21588	0.5261569823527318	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21387	0.521258077708814	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	18090	0.44090141795260884	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	16557	0.40353813029526503	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15752	0.38391813905967354	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	14447	0.3521118178640873	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	14313	0.34884588143480877	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13873	0.3381219110700134	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	13324	0.3247413207739392	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	12457	0.30361022462330833	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	12346	0.3009048593721895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11853	0.28888913803163474	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	10885	0.26529640322908493	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	10764	0.2623473113787662	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	10685	0.2604218712450871	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10550	0.2571315621558885	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	8948	0.21808656096406545	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	8780	0.2139919540975072	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	8508	0.2073625905992701	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	8009	0.19520063329919535	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	7934	0.19337268380519615	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	7808	0.1903017286552775	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	7656	0.18659708434743907	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	7140	0.17402079182872454	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	6985	0.1702430295411262	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	6912	0.16846382536696694	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	6487	0.15810544490097145	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	6343	0.15459578187249295	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	6133	0.1494775232892952	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	5979	0.1457241336616168	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	5577	0.13592632437378105	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	5377	0.13105179238978318	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5181	0.12627475104546526	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	5172	0.12605539710618535	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	5076	0.12371562175386636	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	5056	0.12322816855546657	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	4856	0.11835363657146869	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4493	0.10950636102051252	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	4441	0.10823898270467305	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4365	0.10638666055075388	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	4354	0.106118561291634	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	4315	0.10516802755475439	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	4173	0.10170710984611589	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	4133	0.10073220344931633	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	4121	0.10043973153027645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.311797975996829E-5
2	0.0	0.0	0.0	0.0	7.311797975996829E-5
3	0.0	0.0	0.0	0.0	7.311797975996829E-5
4	0.0	0.0	0.0	2.4372659919989432E-5	7.311797975996829E-5
5	0.0	0.0	0.0	2.4372659919989432E-5	7.311797975996829E-5
6	0.0	0.0	0.0	2.4372659919989432E-5	7.311797975996829E-5
7	0.0	0.0	0.0	2.4372659919989432E-5	7.311797975996829E-5
8	0.0	0.0	0.0	4.8745319839978865E-5	7.311797975996829E-5
9	0.0	0.0	0.0	1.2186329959994715E-4	9.749063967995773E-5
10	0.0	0.0	2.4372659919989432E-5	1.9498127935991546E-4	9.749063967995773E-5
11	0.0	0.0	2.4372659919989432E-5	2.6809925911988375E-4	9.749063967995773E-5
12	0.0	0.0	2.4372659919989432E-5	5.361985182397675E-4	9.749063967995773E-5
13	0.0	0.0	2.4372659919989432E-5	6.824344777597041E-4	9.749063967995773E-5
14	0.0	0.0	2.4372659919989432E-5	0.0011698876761594926	1.4623595951993658E-4
15	2.4372659919989432E-5	0.0	2.4372659919989432E-5	0.002022930773359123	1.4623595951993658E-4
16	2.4372659919989432E-5	0.0	2.4372659919989432E-5	0.004387078785598098	1.9498127935991546E-4
17	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.007604269895036703	2.193539392799049E-4
18	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.009822181947755741	3.168445789598626E-4
19	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.014501732652393712	3.168445789598626E-4
20	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.01898630207767177	3.168445789598626E-4
21	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.030075862341266957	3.168445789598626E-4
22	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.043992651155580924	3.168445789598626E-4
23	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.062125910136053064	3.4121723887985204E-4
24	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.09049568628292076	3.4121723887985204E-4
25	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.108409591324113	3.4121723887985204E-4
26	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.1437012028882577	3.899625587198309E-4
27	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.2221080498508637	3.899625587198309E-4
28	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.355158400354086	3.899625587198309E-4
29	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.47816721497027265	3.899625587198309E-4
30	4.8745319839978865E-5	0.0	2.4372659919989432E-5	0.6599141399936339	3.899625587198309E-4
31	4.8745319839978865E-5	0.0	2.4372659919989432E-5	1.0823167090669708	3.899625587198309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	1925	0.0	16.625975	2
GCGCTAG	90	4.4504377E-5	16.444445	26
AAAAAGT	1990	0.0	16.082914	1
ACGAACG	880	0.0	15.346591	15
TGGGCTG	3565	0.0	15.152876	36
AAGACGG	1380	0.0	15.148551	5
TATACTG	375	0.0	14.799999	5
AACGAAC	935	0.0	14.64171	14
CGAACGA	920	0.0	14.478261	16
CGCTAGG	115	2.2124716E-5	14.478261	27
CAAGACG	1405	0.0	14.352313	4
ATAACGA	955	0.0	14.335078	12
TAACGAA	945	0.0	14.291004	13
TCGTTAC	365	0.0	14.191781	23
GGGCTGT	4085	0.0	14.175031	37
ACCGTCG	995	0.0	13.944723	8
ATAAGAC	280	0.0	13.875001	3
GTTACGG	430	0.0	13.767442	25
GTAATAC	175	3.5732228E-8	13.742858	3
AGTACTC	2425	0.0	13.731959	5
>>END_MODULE