FastQCFastQC Report
Thu 9 Feb 2017
SRR2727185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6548417
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC554920.8474109086211218No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG551320.8419133967797103No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA358490.5474452833409967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT333460.5092223051769611No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG297540.45436935369265574No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG253680.38739133442479307No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC247950.3786411280772132No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT221200.3377915609222809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218630.3338669483021622No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT205380.31363305055252283No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG205360.31360250882007057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185250.2828927968392972No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC180800.27609726136866364No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT176410.26939335109538687No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG170710.2606889573464854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162790.24859443129538025No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA157580.24063830999155977No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC152600.23303341861094065No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT144990.2214122894128459No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT126030.19245872704807895No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT124210.18967942939492094No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC117050.17874548917700261No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT112000.17103370173280047No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC111110.16967459463867374No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT110900.16935390644792475No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC109390.16704800564777716No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC98220.14999044807317555No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA97020.1481579441260384No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT96550.14744021341340968No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT93820.14327126693367268No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG86760.1324900353780158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC84100.12842798496186178No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82180.12549597864644235No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG81530.12450337234174305No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT77580.11847138018241661No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT74460.11370686991986002No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT71620.10936994391163544No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70210.10721675177374929No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC69880.10671281318828658No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG69170.10562858168623043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17500.015.545
AAAAGTA36050.014.9847432
CAAGACG18400.014.7798924
AAAAAGT35800.014.7276541
AGTACTC39250.014.0929935
CTTATAC76950.013.9441237
AAGTACT41000.013.7621964
AAAGTAC39850.013.69510653
GTATTAC3450.013.4057961
TGGGCTG55250.013.36018136
ATACCGT17050.013.1290326
ACCGTCG15950.013.1065848
GGGCTGT58950.012.99236637
TACCGTC16850.012.9554897
CGTATAC1153.5811664E-412.8695653
ACTCTAA20400.012.78676410
TCGTTAC6350.012.52755923
TATCACG2552.237357E-1012.3333332
GTATTAG16800.012.3333331
CACTCTA21150.012.3333339
Produced by FastQC (version 0.11.5)