##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6548417
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175173175440722	31.0	31.0	33.0	30.0	34.0
2	31.285775172839482	31.0	31.0	34.0	30.0	34.0
3	31.34331121551972	31.0	31.0	34.0	28.0	34.0
4	35.169405674684434	35.0	35.0	37.0	33.0	37.0
5	35.02764484912919	35.0	35.0	37.0	32.0	37.0
6	35.07772031011464	36.0	35.0	37.0	32.0	37.0
7	34.88887207396841	36.0	35.0	37.0	32.0	37.0
8	34.81995893053237	36.0	35.0	37.0	32.0	37.0
9	36.451576464968554	38.0	35.0	39.0	32.0	39.0
10	36.23271211958554	38.0	35.0	39.0	32.0	39.0
11	36.35720266439966	38.0	35.0	39.0	32.0	39.0
12	36.22682000856085	38.0	35.0	39.0	32.0	39.0
13	36.324763832236094	38.0	35.0	39.0	32.0	39.0
14	37.29457974347083	39.0	36.0	41.0	32.0	41.0
15	37.16946767440131	39.0	36.0	41.0	31.0	41.0
16	37.10507684528948	39.0	36.0	40.0	31.0	41.0
17	37.045559865842385	39.0	36.0	40.0	31.0	41.0
18	37.038418139834405	39.0	36.0	40.0	31.0	41.0
19	37.058408161850416	39.0	36.0	40.0	31.0	41.0
20	36.96657451716957	39.0	36.0	40.0	31.0	41.0
21	36.83832688113784	39.0	36.0	40.0	31.0	41.0
22	36.812172468552326	39.0	35.0	40.0	30.0	41.0
23	36.59899300853932	38.0	35.0	40.0	30.0	41.0
24	36.47822153048592	38.0	35.0	40.0	30.0	41.0
25	36.376971258855384	38.0	35.0	40.0	30.0	41.0
26	36.03365729457974	38.0	34.0	40.0	29.0	41.0
27	36.172534522465504	38.0	35.0	40.0	29.0	41.0
28	36.02614341756183	38.0	35.0	40.0	29.0	41.0
29	35.93581135715701	38.0	34.0	40.0	28.0	41.0
30	36.04596912505725	38.0	35.0	40.0	29.0	41.0
31	35.97321261000941	38.0	35.0	40.0	29.0	41.0
32	35.746598452725294	38.0	34.0	40.0	27.0	41.0
33	35.684738464273124	38.0	34.0	40.0	27.0	41.0
34	35.68782134674686	38.0	34.0	40.0	27.0	41.0
35	35.407524597166	38.0	34.0	40.0	26.0	41.0
36	35.274665006825316	38.0	34.0	40.0	26.0	41.0
37	35.049427670840146	38.0	33.0	40.0	25.0	41.0
38	34.90811244916138	38.0	33.0	40.0	25.0	41.0
39	34.84560008930403	38.0	33.0	40.0	24.0	41.0
40	34.70767774868339	38.0	33.0	40.0	24.0	41.0
41	34.54923563969735	38.0	33.0	40.0	24.0	41.0
42	34.38393339947655	38.0	33.0	40.0	23.0	41.0
43	33.93581593841687	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	6.0
13	12.0
14	29.0
15	77.0
16	221.0
17	496.0
18	986.0
19	2029.0
20	4009.0
21	7339.0
22	12946.0
23	21242.0
24	33375.0
25	50706.0
26	72351.0
27	102206.0
28	137255.0
29	178925.0
30	223920.0
31	271550.0
32	324933.0
33	386473.0
34	463156.0
35	552238.0
36	669561.0
37	844018.0
38	1100813.0
39	1087544.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.70494685967616	19.207222142389526	12.386489742482802	25.70134125545151
2	16.44450864995311	24.89917792345845	32.92502600246747	25.731287424120975
3	21.15935805554228	26.456806278525026	29.22084833632311	23.16298732960958
4	15.506297170751344	17.520661863775626	32.37887874275569	34.59416222271734
5	13.814621151951686	37.65229062229849	33.677956061747444	14.855132164002386
6	33.55302510515137	32.582378306085275	17.097231285057138	16.767365303706224
7	25.877979365089303	31.190576287368383	23.33644909907234	19.594995248469974
8	27.441120502863512	32.12993308153711	22.92830465744622	17.500641758153154
9	27.865421520956897	14.021495576717244	20.20019494787824	37.91288795444762
10	17.927172322715553	26.776150633046125	33.11049067278397	22.186186371454355
11	33.81692094440535	22.610884432069614	20.822253683600174	22.74994093992487
12	21.411770203394195	27.24504258051984	29.522982424607353	21.820204791478613
13	30.755326058190857	18.993292577427493	25.297961324087943	24.953420040293707
14	22.72540065789946	22.03305012493859	25.930709666168173	29.310839550993773
15	27.73439748873659	25.113825219133112	23.630489628256722	23.521287663873576
16	23.082937448852142	25.420235149960668	28.513288020601006	22.98353938058618
17	24.619598904590223	27.01610175405751	26.742066059629373	21.622233281722895
18	25.11075577502166	22.452983675291296	29.330462614094365	23.10579793559268
19	24.010169175237316	28.334511989691553	27.317243236037047	20.338075599034084
20	23.405244351421114	24.36784340398603	28.68626112234453	23.540651122248324
21	22.83438883015544	27.257274544366982	27.4946754307186	22.413661194758976
22	23.690534674257915	26.737316820233044	24.48796403772087	25.084184467788166
23	20.935639865329282	25.923471275576983	26.90835052196584	26.232538337127885
24	21.882479383948823	29.663642373416355	26.024152096605942	22.42972614602888
25	23.943557656758877	25.52705485921254	24.14333112872928	26.386056355299303
26	23.00285702636225	26.13706488148204	27.039939576236517	23.82013851591919
27	25.005295172863917	25.470522112443362	24.94770873632513	24.576473978367595
28	22.510463215766496	26.458363906880088	27.563058369679265	23.468114507674144
29	23.27116614595558	25.111610943530323	28.019428206847547	23.59779470366655
30	22.46765897773462	27.567318941356362	28.45457153996149	21.510450540947527
31	25.215162687409798	25.51091355361151	24.493583716492093	24.780340042486603
32	20.29569894525654	27.061792185806127	27.127136222387794	25.51537264654954
33	22.197105040806044	26.18338141874593	27.68893611998136	23.930577420466655
34	21.910501423473796	26.08723604498614	27.71604190753277	24.2862206240073
35	22.088620807135527	27.337675655047622	28.721658990256728	21.85204454756012
36	23.563603234186218	26.07900504809025	26.623579408580728	23.733812309142806
37	22.21949213069357	26.31428328403643	27.932811853612865	23.533412731657133
38	22.916637715649447	25.50630175201121	28.236808987576694	23.34025154476265
39	23.152129743722796	23.18338920688771	27.845828999588758	25.81865204980074
40	22.59564410757592	25.060682604666134	28.620901814896637	23.722771472861304
41	21.01907987838893	24.844340242840367	29.183663777062456	24.952916101708244
42	22.88313343514929	25.717238227192922	28.612716630599426	22.786911707058362
43	21.687302442712493	23.345061867623883	29.479536810193974	25.48809887946965
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2151.0
1	2368.5
2	2586.0
3	8059.5
4	13533.0
5	13533.0
6	18895.0
7	24257.0
8	24123.0
9	23989.0
10	28212.5
11	32436.0
12	32436.0
13	46218.0
14	60000.0
15	78171.0
16	96342.0
17	87457.5
18	78573.0
19	78573.0
20	78693.5
21	78814.0
22	67644.5
23	56475.0
24	59882.5
25	63290.0
26	63290.0
27	69080.0
28	74870.0
29	80089.0
30	85308.0
31	94824.5
32	104341.0
33	104341.0
34	123653.5
35	142966.0
36	173378.5
37	203791.0
38	233031.0
39	262271.0
40	262271.0
41	283418.5
42	304566.0
43	337042.0
44	369518.0
45	435305.5
46	501093.0
47	501093.0
48	729624.5
49	958156.0
50	941811.0
51	925466.0
52	689746.5
53	454027.0
54	454027.0
55	410135.5
56	366244.0
57	352863.0
58	339482.0
59	315102.0
60	290722.0
61	290722.0
62	242181.5
63	193641.0
64	169164.0
65	144687.0
66	123360.0
67	102033.0
68	102033.0
69	85932.5
70	69832.0
71	60750.0
72	51668.0
73	38742.5
74	25817.0
75	25817.0
76	20253.0
77	14689.0
78	12972.0
79	11255.0
80	9407.0
81	7559.0
82	7559.0
83	6442.5
84	5326.0
85	4713.0
86	4100.0
87	2754.0
88	1408.0
89	1408.0
90	1012.5
91	617.0
92	430.0
93	243.0
94	245.0
95	247.0
96	247.0
97	130.5
98	14.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6548417.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.67927529386531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5417187255104	30.724328677934388
2	9.159574255241013	6.90252239875655
3	3.1669392112206687	3.5798392313556073
4	1.6221918191588605	2.444920485341715
5	0.9527023822832236	1.7948567667585444
6	0.6194583705425247	1.4004445486056607
7	0.4367781034939464	1.152023768271656
8	0.36058459089755357	1.0869252853723776
9	0.2567755938421445	0.8707606460211518
>10	1.613553932739008	11.321916355393956
>50	0.12929301167961552	3.371780889862497
>100	0.1058406418385788	8.348388399870807
>500	0.01776879349739954	4.630062661082814
>1k	0.014470635753309946	10.363781405763222
>5k	0.0012780333571294834	3.324336113180669
>10k+	0.001071898944689244	8.6831123664285
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	55492	0.8474109086211218	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	55132	0.8419133967797103	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	35849	0.5474452833409967	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33346	0.5092223051769611	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	29754	0.45436935369265574	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	25368	0.38739133442479307	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	24795	0.3786411280772132	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22120	0.3377915609222809	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21863	0.3338669483021622	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	20538	0.31363305055252283	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	20536	0.31360250882007057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18525	0.2828927968392972	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	18080	0.27609726136866364	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	17641	0.26939335109538687	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	17071	0.2606889573464854	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16279	0.24859443129538025	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	15758	0.24063830999155977	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	15260	0.23303341861094065	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	14499	0.2214122894128459	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	12603	0.19245872704807895	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	12421	0.18967942939492094	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	11705	0.17874548917700261	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	11200	0.17103370173280047	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	11111	0.16967459463867374	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	11090	0.16935390644792475	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	10939	0.16704800564777716	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	9822	0.14999044807317555	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9702	0.1481579441260384	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	9655	0.14744021341340968	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	9382	0.14327126693367268	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	8676	0.1324900353780158	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	8410	0.12842798496186178	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8218	0.12549597864644235	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	8153	0.12450337234174305	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	7758	0.11847138018241661	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	7446	0.11370686991986002	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	7162	0.10936994391163544	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	7021	0.10721675177374929	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	6988	0.10671281318828658	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	6917	0.10562858168623043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.10834649045716E-5
2	0.0	0.0	0.0	0.0	6.10834649045716E-5
3	0.0	0.0	0.0	0.0	6.10834649045716E-5
4	0.0	0.0	0.0	1.52708662261429E-5	6.10834649045716E-5
5	1.52708662261429E-5	0.0	0.0	1.52708662261429E-5	6.10834649045716E-5
6	1.52708662261429E-5	0.0	0.0	1.52708662261429E-5	6.10834649045716E-5
7	1.52708662261429E-5	0.0	0.0	3.05417324522858E-5	6.10834649045716E-5
8	1.52708662261429E-5	0.0	0.0	4.58125986784287E-5	7.635433113071449E-5
9	1.52708662261429E-5	0.0	0.0	7.635433113071449E-5	9.16251973568574E-5
10	1.52708662261429E-5	1.52708662261429E-5	0.0	1.679795284875719E-4	9.16251973568574E-5
11	1.52708662261429E-5	1.52708662261429E-5	0.0	3.0541732452285796E-4	1.0689606358300029E-4
12	1.52708662261429E-5	1.52708662261429E-5	0.0	7.940850437594307E-4	1.0689606358300029E-4
13	1.52708662261429E-5	1.52708662261429E-5	0.0	9.620645722470026E-4	1.221669298091432E-4
14	3.05417324522858E-5	3.05417324522858E-5	0.0	0.0015881700875188614	1.679795284875719E-4
15	3.05417324522858E-5	3.05417324522858E-5	0.0	0.002397525997504435	1.832503947137148E-4
16	3.05417324522858E-5	3.05417324522858E-5	0.0	0.005115740185757871	1.9852126093985767E-4
17	3.05417324522858E-5	3.05417324522858E-5	0.0	0.00847533075550931	1.9852126093985767E-4
18	3.05417324522858E-5	3.05417324522858E-5	0.0	0.01068960635830003	2.1379212716600058E-4
19	3.05417324522858E-5	4.58125986784287E-5	0.0	0.016187118199711473	2.1379212716600058E-4
20	3.05417324522858E-5	4.58125986784287E-5	0.0	0.020661482003971342	2.1379212716600058E-4
21	3.05417324522858E-5	4.58125986784287E-5	0.0	0.03153433875698509	2.443338596182864E-4
22	3.05417324522858E-5	4.58125986784287E-5	0.0	0.046316537263891414	2.443338596182864E-4
23	4.58125986784287E-5	4.58125986784287E-5	0.0	0.0652829531167609	2.9014645829671505E-4
24	4.58125986784287E-5	4.58125986784287E-5	0.0	0.09478626666566897	3.0541732452285796E-4
25	4.58125986784287E-5	4.58125986784287E-5	0.0	0.11283643054496988	3.2068819074900087E-4
26	4.58125986784287E-5	4.58125986784287E-5	0.0	0.14824956932339525	3.359590569751438E-4
27	4.58125986784287E-5	4.58125986784287E-5	0.0	0.22768861543179061	3.359590569751438E-4
28	4.58125986784287E-5	4.58125986784287E-5	0.0	0.36237765554637097	3.359590569751438E-4
29	4.58125986784287E-5	4.58125986784287E-5	0.0	0.48581206725228404	3.359590569751438E-4
30	4.58125986784287E-5	4.58125986784287E-5	0.0	0.6709560493780405	3.359590569751438E-4
31	4.58125986784287E-5	4.58125986784287E-5	0.0	1.092141810761288	3.359590569751438E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1750	0.0	15.54	5
AAAAGTA	3605	0.0	14.984743	2
CAAGACG	1840	0.0	14.779892	4
AAAAAGT	3580	0.0	14.727654	1
AGTACTC	3925	0.0	14.092993	5
CTTATAC	7695	0.0	13.94412	37
AAGTACT	4100	0.0	13.762196	4
AAAGTAC	3985	0.0	13.6951065	3
GTATTAC	345	0.0	13.405796	1
TGGGCTG	5525	0.0	13.360181	36
ATACCGT	1705	0.0	13.129032	6
ACCGTCG	1595	0.0	13.106584	8
GGGCTGT	5895	0.0	12.992366	37
TACCGTC	1685	0.0	12.955489	7
CGTATAC	115	3.5811664E-4	12.869565	3
ACTCTAA	2040	0.0	12.786764	10
TCGTTAC	635	0.0	12.527559	23
TATCACG	255	2.237357E-10	12.333333	2
GTATTAG	1680	0.0	12.333333	1
CACTCTA	2115	0.0	12.333333	9
>>END_MODULE