FastQCFastQC Report
Thu 9 Feb 2017
SRR2727184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9376455
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC997091.063397627354901No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG965251.029440230876168No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA649770.6929804494342477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT643840.6866560976403129No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG535580.5711966836080374No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG451320.4813332970722944No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC431320.46000327415851733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT425890.45421217293742683No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT413310.44079558852466094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT377900.40303078295581857No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG370790.39544795980997083No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT362730.38685196057571863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312470.3332496129933968No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT306140.32649866074118633No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC287300.30640577915640826No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG285150.3041128016931772No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA274870.2931491699154958No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC272650.2907815373720665No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT265020.28264413363046054No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT220540.23520616267022026No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT208950.2228454143916864No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT205320.21897401523283586No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC200150.21346020430962448No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT199220.21246835824413382No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC196030.20906621958938643No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC195790.20881025931442104No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC181300.19335665771338953No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA176150.18786417681309195No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT160870.17156803930696624No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT159480.17008560271445872No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG157900.16840053090427032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC154030.16427317147045445No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG149150.15906864587949282No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT144680.15430138575826366No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141540.15095257216080066No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT133670.14255920814422934No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC125550.13389921884123585No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT124660.13295003282157275No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG121690.12978252441887686No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG112200.11966142854628961No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC105970.11301712640864804No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104290.11122540448389076No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT103530.11041486361316724No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT102920.10976429791429702No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT101290.1080259010468242No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG101110.10783393084060021No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT100650.10734334031358332No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG99320.10592489378981715No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT98910.10548762832008472No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG98610.10516767797637808No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA98410.10495437774724029No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG98270.10480506758684385No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC97170.10363191632658611No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA94130.100389752843692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG25950.016.3969155
AAAAAGT49550.015.9424821
CAAGACG27050.015.7985214
AAAAGTA51850.015.4493722
TGGGCTG81700.015.21664636
GTCTTAC3900.014.7051291
GGGCTGT87200.014.638761537
TATCACG4550.014.6373632
TCGTTAC6900.014.47826123
AGTACTC59950.014.1025855
AAGTACT61650.013.9237644
CGCAAGA31900.013.6285282
AAAGTAC59400.013.610273
GCGCAAG31150.013.4815421
CGAACGA15250.013.46557416
ACGAACG15350.013.3778515
GACGGAC30950.013.2697917
CACTCTA23800.013.2142869
ACGGACC31550.013.076078
AACGAAC15950.012.87460914
Produced by FastQC (version 0.11.5)