FastQCFastQC Report
Thu 9 Feb 2017
SRR2727183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7882943
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC702300.8909109199444928No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG682530.865831454064808No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA445350.56495397721384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT402400.510469249872795No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG372830.4729578788023711No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC316460.4014490527205385No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG314900.39947009638405356No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT277900.35253331148024286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT260760.3307901630139911No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG259460.3291410327335869No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT251030.31844705714604304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230240.29207365827711806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT213100.2703305098108663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204260.25911642390411804No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC204190.25902762458132705No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG202700.25713746756763306No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA195920.24853661887444828No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC194360.24655766253796327No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT182670.23172817563186743No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT153520.19474960049818957No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT152520.19348103874403252No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC140680.17846126757481312No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT139610.1771039064978651No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC138670.17591145844895745No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC136340.17295570956177153No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT135870.17235948553731772No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC127450.16167819556731539No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA122390.15525927309128074No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT113650.14417204335994818No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT111950.14201548837788122No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG111820.14185057534984077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC105290.13356686709519527No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG104960.13314824171632347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104580.13266618824974377No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT99560.1262980082438754No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT96500.12241620927615486No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG89230.11319376532343314No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT87420.11089766854840888No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC87350.11080886922561789No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG82730.10494811392141234No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81590.10350195352167332No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG80910.10263933152884652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT40300.016.1129021
AAAAGTA41200.016.0752412
AAGACGG23500.015.1936175
CAAGACG24350.014.9671464
ACCGTCG18400.013.9755428
AGTACTC49150.013.96439555
ATACCGT19250.013.9350656
AAGTACT52100.013.4932824
TACCGTC19500.013.4717957
GTACCGT2750.013.4545466
GTATTAC4550.013.4175821
TGGGCTG65800.013.18617136
AAAGTAC51150.012.984363
AGGTTAT16050.012.7943931
GGGCTGT70450.012.68346337
GACGGAC28450.012.6801417
GTTACGG6600.012.61363625
GTAAACG20100.012.60945227
CGCTTCG19700.012.58375632
TATACTG7500.012.5800015
Produced by FastQC (version 0.11.5)