##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727183.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7882943
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19668796285854	31.0	31.0	33.0	30.0	34.0
2	31.30895288726558	31.0	31.0	34.0	30.0	34.0
3	31.375883347120485	31.0	31.0	34.0	28.0	34.0
4	35.195441727791255	35.0	35.0	37.0	33.0	37.0
5	35.05341672520022	35.0	35.0	37.0	32.0	37.0
6	35.107468111846046	36.0	35.0	37.0	32.0	37.0
7	34.920406629858924	36.0	35.0	37.0	32.0	37.0
8	34.85871571061721	36.0	35.0	37.0	32.0	37.0
9	36.492915780311996	38.0	35.0	39.0	32.0	39.0
10	36.27801266608169	38.0	35.0	39.0	32.0	39.0
11	36.391272016047814	38.0	35.0	39.0	32.0	39.0
12	36.275235403833314	38.0	35.0	39.0	32.0	39.0
13	36.3654888789631	38.0	35.0	39.0	32.0	39.0
14	37.34983343149887	39.0	36.0	41.0	32.0	41.0
15	37.23023837670779	39.0	36.0	41.0	31.0	41.0
16	37.16631440821023	39.0	36.0	40.0	31.0	41.0
17	37.10884107623257	39.0	36.0	40.0	31.0	41.0
18	37.098799648811365	39.0	36.0	40.0	31.0	41.0
19	37.11064484419081	39.0	36.0	40.0	31.0	41.0
20	37.02088471272722	39.0	36.0	40.0	31.0	41.0
21	36.893536969631775	39.0	36.0	40.0	31.0	41.0
22	36.86859691868887	39.0	36.0	40.0	31.0	41.0
23	36.656467261021675	39.0	35.0	40.0	30.0	41.0
24	36.541574130372375	38.0	35.0	40.0	30.0	41.0
25	36.43615855144456	38.0	35.0	40.0	30.0	41.0
26	36.103198894118606	38.0	35.0	40.0	29.0	41.0
27	36.23787359619371	38.0	35.0	40.0	30.0	41.0
28	36.10481161667666	38.0	35.0	40.0	29.0	41.0
29	36.01391003334668	38.0	35.0	40.0	29.0	41.0
30	36.11246205890364	38.0	35.0	40.0	30.0	41.0
31	36.037008766903426	38.0	35.0	40.0	29.0	41.0
32	35.813896916418145	38.0	34.0	40.0	28.0	41.0
33	35.74982782445592	38.0	34.0	40.0	28.0	41.0
34	35.7467948201579	38.0	34.0	40.0	28.0	41.0
35	35.46606210396295	38.0	34.0	40.0	27.0	41.0
36	35.32703953333165	38.0	34.0	40.0	26.0	41.0
37	35.09905627885423	38.0	34.0	40.0	25.0	41.0
38	34.953734030551786	38.0	33.0	40.0	25.0	41.0
39	34.89618115975214	38.0	33.0	40.0	24.0	41.0
40	34.74978202937659	38.0	33.0	40.0	24.0	41.0
41	34.587138458314364	38.0	33.0	40.0	24.0	41.0
42	34.4247747319751	38.0	33.0	40.0	23.0	41.0
43	33.974831100516646	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	12.0
13	17.0
14	25.0
15	92.0
16	259.0
17	595.0
18	1156.0
19	2365.0
20	4646.0
21	8657.0
22	14977.0
23	24698.0
24	38694.0
25	58559.0
26	84612.0
27	119005.0
28	162060.0
29	211927.0
30	266009.0
31	323007.0
32	385513.0
33	460500.0
34	552176.0
35	660704.0
36	806278.0
37	1020920.0
38	1330663.0
39	1344813.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.001465315682225	19.091410403449576	12.239350202075544	25.667774078792654
2	16.528065723677056	24.803604440625794	32.81388689478029	25.85444294091686
3	21.31136810198932	26.383927931484475	29.04793298644935	23.25677098007686
4	15.67919494026533	17.43844906654786	32.05475416985763	34.82760182332918
5	13.939527407466985	37.454602931925294	33.58688499967588	15.018984660931837
6	33.76392547808604	32.18781361225116	17.095353854518546	16.952907055144255
7	26.023263139160086	30.931417365316484	23.342779467008704	19.702540028514733
8	27.46711475650655	31.937716662419096	22.929976786588462	17.665191794485892
9	27.740388329587056	14.022263005073105	20.306629135844318	37.93071952949552
10	18.080899481323158	26.665434470349464	33.01369805667756	22.23996799164982
11	33.69086900666414	22.488720266022476	20.847188670525714	22.973222056787673
12	21.56713552286246	27.21408742902238	29.46886714771374	21.749909900401413
13	30.75069805781927	18.86868901627222	25.312107419779643	25.06850550612886
14	22.746187559646188	21.97754062156735	25.924366064805998	29.35190575398046
15	27.701544968674767	24.936169143935203	23.650025123865543	23.712260763524487
16	23.021592316473683	25.304369193079285	28.608718850307557	23.065319640139474
17	24.68564088310673	27.000702656355628	26.700637059027322	21.613019401510325
18	25.139265880775746	22.320889545947498	29.31495255008187	23.22489202319489
19	23.957625978013542	28.25760632799197	27.318604231947386	20.466163462047106
20	23.46156758966797	24.323758271498345	28.688498699026493	23.52617543980719
21	22.79936566838045	27.117384966502993	27.57273520815766	22.51051415695889
22	23.70024748371262	26.748550636481834	24.46366794736433	25.087533932441218
23	20.932182308054237	25.915118249618196	26.923599472937966	26.22909996938961
24	21.99497066006947	29.66928721925301	25.9867412462579	22.349000874419616
25	23.920355633676408	25.5221051325628	24.138091573159922	26.41944766060087
26	23.08358692939934	26.150981936568613	26.967720050747545	23.797711083284504
27	24.862440334783596	25.432481244631607	24.940076314138008	24.765002106446794
28	22.449635878376895	26.49866934214798	27.627283363586418	23.42441141588871
29	23.323167502289436	24.994040931159848	27.977685491319676	23.70510607523104
30	22.289137445240947	27.63831985084758	28.5919484639176	21.48059423999387
31	25.34509002538773	25.44354310312785	24.38226433960007	24.82910253188435
32	20.255341184123747	27.046726076796446	27.113909107296603	25.584023631783204
33	22.14231410781481	26.26628151440395	27.725622270768667	23.86578210701257
34	21.867873457920474	26.089773831930536	27.61034806416842	24.43200464598057
35	21.969979993512574	27.40056600688347	28.73353264129907	21.895921358304886
36	23.536450790015863	26.1122527462142	26.541407187645525	23.809889276124412
37	22.143684154509298	26.358341040903127	27.882226219319357	23.615748585268218
38	22.758695578542177	25.638191218685712	28.244527456306614	23.3585857464655
39	23.139949128136536	23.20841340600839	27.77888917882573	25.872748287029353
40	22.4493314235559	25.22687275551783	28.548309939574594	23.775485881351674
41	20.995851422495377	24.914197654353202	29.046271170551403	25.043679752600013
42	22.848344837708453	25.849939546689605	28.47120675615693	22.830508859445008
43	21.703429797729097	23.423891305569505	29.357728452432042	25.514950444269353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2423.0
1	2694.0
2	2965.0
3	10121.5
4	17278.0
5	17278.0
6	24188.0
7	31098.0
8	30885.5
9	30673.0
10	36054.0
11	41435.0
12	41435.0
13	58708.5
14	75982.0
15	97956.5
16	119931.0
17	108503.5
18	97076.0
19	97076.0
20	97271.5
21	97467.0
22	83115.0
23	68763.0
24	71977.0
25	75191.0
26	75191.0
27	81113.5
28	87036.0
29	91932.0
30	96828.0
31	106337.0
32	115846.0
33	115846.0
34	137427.5
35	159009.0
36	193834.5
37	228660.0
38	265907.0
39	303154.0
40	303154.0
41	329155.5
42	355157.0
43	396316.5
44	437476.0
45	517189.5
46	596903.0
47	596903.0
48	875489.0
49	1154075.0
50	1145016.0
51	1135957.0
52	846625.0
53	557293.0
54	557293.0
55	505765.5
56	454238.0
57	435982.0
58	417726.0
59	387811.0
60	357896.0
61	357896.0
62	298773.0
63	239650.0
64	208610.0
65	177570.0
66	150570.0
67	123570.0
68	123570.0
69	103276.5
70	82983.0
71	71427.5
72	59872.0
73	44846.0
74	29820.0
75	29820.0
76	23423.5
77	17027.0
78	14922.0
79	12817.0
80	10633.0
81	8449.0
82	8449.0
83	7148.5
84	5848.0
85	5269.5
86	4691.0
87	3195.0
88	1699.0
89	1699.0
90	1211.0
91	723.0
92	529.5
93	336.0
94	324.5
95	313.0
96	313.0
97	168.5
98	24.0
99	19.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7882943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.99680439653922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62180215617633	29.021272423123634
2	9.494506230161312	6.835437672176795
3	3.403106665474161	3.675028949328966
4	1.665192659450636	2.3976645777919
5	1.0304352329251827	1.8546187761455064
6	0.6491905639717533	1.402127144842211
7	0.473476397745749	1.193054609322221
8	0.3545685976330653	1.0210669163322135
9	0.26979434080252795	0.8740560701845655
>10	1.7571117573188082	11.949491830339145
>50	0.13958160369019829	3.4772334289751865
>100	0.10540365115318512	7.880355640768319
>500	0.018040635438081435	4.527783829596116
>1k	0.01527889200246694	10.629498269588174
>5k	0.0012911739720039477	3.1747020523765377
>10k+	0.001219442084670395	10.086607809108653
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	70230	0.8909109199444928	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	68253	0.865831454064808	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	44535	0.56495397721384	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40240	0.510469249872795	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	37283	0.4729578788023711	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	31646	0.4014490527205385	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	31490	0.39947009638405356	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	27790	0.35253331148024286	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26076	0.3307901630139911	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	25946	0.3291410327335869	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	25103	0.31844705714604304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23024	0.29207365827711806	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	21310	0.2703305098108663	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20426	0.25911642390411804	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	20419	0.25902762458132705	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	20270	0.25713746756763306	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	19592	0.24853661887444828	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	19436	0.24655766253796327	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	18267	0.23172817563186743	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	15352	0.19474960049818957	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	15252	0.19348103874403252	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	14068	0.17846126757481312	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	13961	0.1771039064978651	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	13867	0.17591145844895745	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	13634	0.17295570956177153	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	13587	0.17235948553731772	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	12745	0.16167819556731539	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	12239	0.15525927309128074	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	11365	0.14417204335994818	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	11195	0.14201548837788122	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	11182	0.14185057534984077	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	10529	0.13356686709519527	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	10496	0.13314824171632347	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10458	0.13266618824974377	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	9956	0.1262980082438754	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	9650	0.12241620927615486	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	8923	0.11319376532343314	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	8742	0.11089766854840888	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	8735	0.11080886922561789	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8273	0.10494811392141234	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8159	0.10350195352167332	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	8091	0.10263933152884652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2685617541570451E-5
2	0.0	0.0	0.0	0.0	2.5371235083140903E-5
3	0.0	0.0	0.0	0.0	2.5371235083140903E-5
4	0.0	1.2685617541570451E-5	0.0	0.0	2.5371235083140903E-5
5	0.0	1.2685617541570451E-5	0.0	0.0	2.5371235083140903E-5
6	1.2685617541570451E-5	1.2685617541570451E-5	0.0	1.2685617541570451E-5	5.0742470166281806E-5
7	1.2685617541570451E-5	1.2685617541570451E-5	0.0	1.2685617541570451E-5	5.0742470166281806E-5
8	1.2685617541570451E-5	1.2685617541570451E-5	0.0	1.2685617541570451E-5	5.0742470166281806E-5
9	1.2685617541570451E-5	1.2685617541570451E-5	0.0	5.0742470166281806E-5	1.268561754157045E-4
10	1.2685617541570451E-5	1.2685617541570451E-5	0.0	1.1417055787413407E-4	1.268561754157045E-4
11	1.2685617541570451E-5	1.2685617541570451E-5	0.0	2.2834111574826814E-4	1.268561754157045E-4
12	1.2685617541570451E-5	1.2685617541570451E-5	0.0	5.32795936745959E-4	1.268561754157045E-4
13	1.2685617541570451E-5	1.2685617541570451E-5	0.0	6.850233472448044E-4	1.5222741049884542E-4
14	1.2685617541570451E-5	1.2685617541570451E-5	0.0	0.0011670768138244815	2.1565549820669767E-4
15	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.002219983069774829	2.4102673328983858E-4
16	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.0052137888095854555	2.4102673328983858E-4
17	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.008245651402020793	2.4102673328983858E-4
18	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.010440263236712481	2.53712350831409E-4
19	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.016364446628625882	2.53712350831409E-4
20	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.021667034761002332	2.53712350831409E-4
21	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.03286843505020904	2.663979683729795E-4
22	2.5371235083140903E-5	2.5371235083140903E-5	0.0	0.0498417913208303	2.663979683729795E-4
23	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.07021489309259245	3.425116736224022E-4
24	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.10205579312193429	3.425116736224022E-4
25	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.12146478796053707	3.551972911639726E-4
26	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.15772028289434542	3.551972911639726E-4
27	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.24181324157741596	3.551972911639726E-4
28	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.3856554588812833	3.551972911639726E-4
29	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.5129429452934012	3.551972911639726E-4
30	2.5371235083140903E-5	3.8056852624711356E-5	0.0	0.7040010310869937	3.551972911639726E-4
31	2.5371235083140903E-5	3.8056852624711356E-5	0.0	1.1010481745206073	3.8056852624711356E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGT	4030	0.0	16.112902	1
AAAAGTA	4120	0.0	16.075241	2
AAGACGG	2350	0.0	15.193617	5
CAAGACG	2435	0.0	14.967146	4
ACCGTCG	1840	0.0	13.975542	8
AGTACTC	4915	0.0	13.9643955	5
ATACCGT	1925	0.0	13.935065	6
AAGTACT	5210	0.0	13.493282	4
TACCGTC	1950	0.0	13.471795	7
GTACCGT	275	0.0	13.454546	6
GTATTAC	455	0.0	13.417582	1
TGGGCTG	6580	0.0	13.186171	36
AAAGTAC	5115	0.0	12.98436	3
AGGTTAT	1605	0.0	12.794393	1
GGGCTGT	7045	0.0	12.683463	37
GACGGAC	2845	0.0	12.680141	7
GTTACGG	660	0.0	12.613636	25
GTAAACG	2010	0.0	12.609452	27
CGCTTCG	1970	0.0	12.583756	32
TATACTG	750	0.0	12.580001	5
>>END_MODULE