FastQCFastQC Report
Thu 9 Feb 2017
SRR2727182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7708674
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC689990.8950826043493343No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG656020.8510153626940249No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA449840.5835504264416942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT418700.543154373891022No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG357860.4642302943411539No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG323610.4197998254952797No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC298470.38718721274242496No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT275920.3579344515022947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT275000.356740990733296No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG261910.3397601195743911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT244570.317265978558699No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT242220.31421746463788713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211210.2739900532828344No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT210580.27317279210406353No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA198420.25739835411382034No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC184470.23930185658389497No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT177690.23050656961236135No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG177060.22968930843359053No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC176070.22840504086695068No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT149550.19400223696059787No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT145150.18829438110886515No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC137450.17830563336833286No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT135670.1759965462283137No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC135480.17575007063471618No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT133280.17289614270884981No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC124380.16135070700875404No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC121280.15732926311321507No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA116830.15155654526316717No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG109390.14190507991387363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT109000.14139915632701552No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104190.13515943208909859No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC103910.13479620489853378No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG98800.128167308670726No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT98220.12741490949027032No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT96110.12467773316137121No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT90870.11788019573794405No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG86380.11205558828924404No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC83880.10881248837348681No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT83560.10839737158426987No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG80960.10502454767188235No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80310.10418134169378546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG19850.015.8438295
AAAAGTA43850.014.8506282
AAAAAGT43050.014.8257851
AGTACTC46200.014.6558455
CAAGACG21800.014.596334
TGGGCTG60600.014.22607336
TAGACCG2151.9826984E-1013.7674425
AAGTACT48750.013.7374354
GGTTATC15650.013.594252
AAAGTAC48050.013.5910513
GTAATAC3700.013.53
GACGGAC23300.013.4184557
GGGCTGT68000.013.38529537
TCGTTAC5550.013.33333223
CGAGCCG20750.013.10602415
ACGGACC23550.013.0403398
CGGACCA24200.012.9194229
TAGACGG1451.3742685E-512.7586215
CTTATAC92900.012.72497337
CTAGCGG13150.012.66159729
Produced by FastQC (version 0.11.5)