##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727182.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7708674
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188203574311224	31.0	31.0	33.0	30.0	34.0
2	31.30023295835315	31.0	31.0	34.0	30.0	34.0
3	31.36265355105171	31.0	31.0	34.0	28.0	34.0
4	35.1826570951113	35.0	35.0	37.0	33.0	37.0
5	35.04227471546987	35.0	35.0	37.0	32.0	37.0
6	35.09794109855988	36.0	35.0	37.0	32.0	37.0
7	34.91080657970489	36.0	35.0	37.0	32.0	37.0
8	34.84911477641939	36.0	35.0	37.0	32.0	37.0
9	36.48102747632083	38.0	35.0	39.0	32.0	39.0
10	36.265378974386515	38.0	35.0	39.0	32.0	39.0
11	36.385215148545655	38.0	35.0	39.0	32.0	39.0
12	36.26192442435625	38.0	35.0	39.0	32.0	39.0
13	36.357539701380546	38.0	35.0	39.0	32.0	39.0
14	37.33393071233782	39.0	36.0	41.0	32.0	41.0
15	37.21977878426303	39.0	36.0	41.0	31.0	41.0
16	37.150007121847416	39.0	36.0	40.0	31.0	41.0
17	37.10036317530096	39.0	36.0	40.0	31.0	41.0
18	37.094552059147915	39.0	36.0	40.0	31.0	41.0
19	37.10929571026094	39.0	36.0	40.0	31.0	41.0
20	37.02385844828825	39.0	36.0	40.0	31.0	41.0
21	36.89135459613417	39.0	36.0	40.0	31.0	41.0
22	36.86761510475083	39.0	36.0	40.0	31.0	41.0
23	36.65179069707708	39.0	35.0	40.0	30.0	41.0
24	36.544439030629654	38.0	35.0	40.0	30.0	41.0
25	36.43701069730021	38.0	35.0	40.0	30.0	41.0
26	36.10069799293627	38.0	35.0	40.0	29.0	41.0
27	36.234407370191036	38.0	35.0	40.0	30.0	41.0
28	36.10326951172147	38.0	35.0	40.0	29.0	41.0
29	36.01185625439602	38.0	35.0	40.0	29.0	41.0
30	36.11058322092749	38.0	35.0	40.0	30.0	41.0
31	36.03817777220829	38.0	35.0	40.0	29.0	41.0
32	35.80377533152913	38.0	34.0	40.0	28.0	41.0
33	35.74761820255987	38.0	34.0	40.0	28.0	41.0
34	35.736649390024795	38.0	34.0	40.0	28.0	41.0
35	35.46208673502083	38.0	34.0	40.0	27.0	41.0
36	35.32663192139141	38.0	34.0	40.0	26.0	41.0
37	35.10021619801278	38.0	34.0	40.0	25.0	41.0
38	34.95872065675627	38.0	33.0	40.0	25.0	41.0
39	34.89370882722502	38.0	33.0	40.0	24.0	41.0
40	34.7497266066771	38.0	33.0	40.0	24.0	41.0
41	34.58751362426275	38.0	33.0	40.0	24.0	41.0
42	34.426592044234845	38.0	33.0	40.0	23.0	41.0
43	33.9752271272595	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	11.0
14	31.0
15	85.0
16	257.0
17	525.0
18	1150.0
19	2293.0
20	4507.0
21	8269.0
22	14733.0
23	23976.0
24	38737.0
25	57846.0
26	83845.0
27	116902.0
28	159232.0
29	207722.0
30	260981.0
31	316848.0
32	376927.0
33	450234.0
34	538828.0
35	643195.0
36	785275.0
37	997171.0
38	1307898.0
39	1311188.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.885845217997286	19.252922097886096	12.353304861510553	25.50792782260607
2	16.4893209908734	24.90200000674565	33.06152264319388	25.547156359187067
3	21.348081395062238	26.47705688423197	29.107651977499632	23.06720974320616
4	15.622258769796208	17.46239366199686	32.230432886382275	34.68491468182466
5	13.890170994388917	37.70012845270146	33.54103442433809	14.868666128571528
6	33.56886022161529	32.40149992073864	17.123723223994165	16.90591663365191
7	25.928013040893934	31.140829149085825	23.31080546407852	19.620352345941726
8	27.409655668406785	32.16100460338575	22.891161307379193	17.538178420828277
9	27.866673308535294	13.990901677772339	20.326063860010166	37.8163611536822
10	18.01104833334501	26.90670276107149	33.03348928752208	22.04875961806142
11	33.582688799656076	22.67478168099987	20.879232926441045	22.86329659290301
12	21.483668916340214	27.370712524618373	29.547909277263507	21.597709281777906
13	30.65130267540176	19.070153959033682	25.400892552986416	24.877650812578143
14	22.682617010396342	22.092736052919086	26.171894154558878	29.052752782125697
15	27.6461425142638	25.093706128965888	23.68312630680711	23.577025049963197
16	22.749463785859927	25.526465381724535	28.718739435602025	23.00533139681351
17	24.569686044577836	27.08865363874513	26.82623496596172	21.51542535071531
18	24.847723486555534	22.61026993747563	29.48569364847962	23.056312927489213
19	23.914644723593188	28.140040686634304	27.672165147987837	20.27314944178467
20	23.366962982219768	24.4793462533245	28.787908270605296	23.365782493850435
21	22.6361758196027	27.125326612592517	27.955650997824012	22.282846569980777
22	23.64380696342847	26.783322267876418	24.704586028673674	24.868284740021434
23	20.93330188823655	26.04516678225075	27.217352815802045	25.804178513710657
24	21.906076194167763	29.619335309808147	26.238507426828534	22.236081069195556
25	23.752087583415772	25.59668498110051	24.462521050961552	26.188706384522163
26	23.012154879036263	26.2019901217771	27.234632052153195	23.55122294703343
27	24.64662534697926	25.515892875999167	25.17997258672503	24.65750919029654
28	22.32263291974729	26.690893920277343	27.801928061817115	23.184545098158257
29	23.289686397427108	25.03287854694595	28.132879922020308	23.544555133606636
30	22.216933807292925	27.769419228261565	28.677513149472915	21.336133814972587
31	25.283557198034316	25.4171080525652	24.751053164266644	24.54828158513384
32	20.140363958833905	27.165087017559696	27.469938409640882	25.224610613965513
33	22.105306308192564	26.347605307994606	27.977911635645768	23.569176748167063
34	21.75101710099558	26.106188950265636	27.84909570699189	24.293698241746895
35	21.738200370128506	27.604540028544466	28.902610228425797	21.754649372901227
36	23.44456387700401	26.1556397377811	26.78238825510068	23.617408130114207
37	22.03912112511179	26.454679494813245	28.058976161140038	23.44722321893493
38	22.768377544568626	25.721557300256826	28.425135114028688	23.084930041145856
39	23.00712158796701	23.355158098526413	27.95491157104322	25.682808742463358
40	22.358903749205115	25.294492930950252	28.755010265059855	23.59159305478478
41	20.972141252827658	25.020295319272805	29.18743223542726	24.82013119247227
42	22.641481531064876	26.009804020769327	28.57788512006086	22.770829328104938
43	21.59066526876088	23.6095857731174	29.522496346323635	25.277252611798083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2490.0
1	2819.5
2	3149.0
3	10196.5
4	17244.0
5	17244.0
6	24112.5
7	30981.0
8	30621.5
9	30262.0
10	36038.5
11	41815.0
12	41815.0
13	59419.0
14	77023.0
15	98959.0
16	120895.0
17	109072.0
18	97249.0
19	97249.0
20	97099.0
21	96949.0
22	82609.5
23	68270.0
24	71464.0
25	74658.0
26	74658.0
27	80842.5
28	87027.0
29	93159.0
30	99291.0
31	110324.5
32	121358.0
33	121358.0
34	143402.5
35	165447.0
36	199229.5
37	233012.0
38	269044.5
39	305077.0
40	305077.0
41	331732.0
42	358387.0
43	397615.0
44	436843.0
45	512751.0
46	588659.0
47	588659.0
48	851961.5
49	1115264.0
50	1110040.0
51	1104816.0
52	823242.0
53	541668.0
54	541668.0
55	488981.0
56	436294.0
57	416854.0
58	397414.0
59	368978.5
60	340543.0
61	340543.0
62	283883.0
63	227223.0
64	197269.0
65	167315.0
66	141462.5
67	115610.0
68	115610.0
69	96304.5
70	76999.0
71	66020.5
72	55042.0
73	41329.0
74	27616.0
75	27616.0
76	21725.5
77	15835.0
78	13635.5
79	11436.0
80	9580.5
81	7725.0
82	7725.0
83	6442.0
84	5159.0
85	4579.5
86	4000.0
87	2712.0
88	1424.0
89	1424.0
90	1022.5
91	621.0
92	454.5
93	288.0
94	271.0
95	254.0
96	254.0
97	141.5
98	29.0
99	21.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7708674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.64108237142974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.13976909835576	29.36407880760213
2	9.824709626563815	7.199759894046068
3	3.3903786425956977	3.7268142934105533
4	1.6817802702424542	2.4648899765039647
5	1.0079023393657514	1.8465316319528615
6	0.682920223168877	1.50137616901476
7	0.49092927767166467	1.2591726071199778
8	0.37159982253488477	1.089265576536751
9	0.2843392558469567	0.9376648285427437
>10	1.8467601522352197	12.742607871203804
>50	0.1448135981407194	3.6641137879810692
>100	0.10175222144893879	7.795582420627994
>500	0.016316842156561807	4.171813443391287
>1k	0.013759407943931897	9.530147392965384
>5k	0.0011166011681828859	2.8669260008937782
>10k+	0.0011526205607049146	9.83925529820706
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	68999	0.8950826043493343	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	65602	0.8510153626940249	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	44984	0.5835504264416942	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41870	0.543154373891022	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	35786	0.4642302943411539	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	32361	0.4197998254952797	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	29847	0.38718721274242496	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	27592	0.3579344515022947	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27500	0.356740990733296	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	26191	0.3397601195743911	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24457	0.317265978558699	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	24222	0.31421746463788713	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21121	0.2739900532828344	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	21058	0.27317279210406353	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	19842	0.25739835411382034	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	18447	0.23930185658389497	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	17769	0.23050656961236135	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	17706	0.22968930843359053	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	17607	0.22840504086695068	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	14955	0.19400223696059787	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	14515	0.18829438110886515	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	13745	0.17830563336833286	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	13567	0.1759965462283137	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	13548	0.17575007063471618	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	13328	0.17289614270884981	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	12438	0.16135070700875404	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	12128	0.15732926311321507	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	11683	0.15155654526316717	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	10939	0.14190507991387363	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	10900	0.14139915632701552	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10419	0.13515943208909859	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	10391	0.13479620489853378	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	9880	0.128167308670726	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	9822	0.12741490949027032	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	9611	0.12467773316137121	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	9087	0.11788019573794405	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	8638	0.11205558828924404	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	8388	0.10881248837348681	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	8356	0.10839737158426987	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8096	0.10502454767188235	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8031	0.10418134169378546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5944799326057893E-5
2	0.0	0.0	0.0	0.0	2.5944799326057893E-5
3	0.0	0.0	0.0	0.0	2.5944799326057893E-5
4	0.0	0.0	0.0	0.0	3.891719898908684E-5
5	0.0	1.2972399663028946E-5	0.0	0.0	3.891719898908684E-5
6	0.0	1.2972399663028946E-5	0.0	0.0	3.891719898908684E-5
7	0.0	1.2972399663028946E-5	0.0	1.2972399663028946E-5	5.1889598652115785E-5
8	0.0	1.2972399663028946E-5	0.0	1.2972399663028946E-5	5.1889598652115785E-5
9	0.0	1.2972399663028946E-5	0.0	2.5944799326057893E-5	9.080679764120262E-5
10	0.0	1.2972399663028946E-5	0.0	1.1675159696726052E-4	9.080679764120262E-5
11	0.0	1.2972399663028946E-5	0.0	2.594479932605789E-4	1.0377919730423157E-4
12	0.0	1.2972399663028946E-5	0.0	5.837579848363026E-4	1.1675159696726052E-4
13	0.0	1.2972399663028946E-5	0.0	7.783439797817368E-4	1.1675159696726052E-4
14	0.0	1.2972399663028946E-5	0.0	0.0014788535615853	1.2972399663028946E-4
15	0.0	1.2972399663028946E-5	0.0	0.002425838736986413	1.5566879595634736E-4
16	0.0	1.2972399663028946E-5	0.0	0.005305711462178839	1.5566879595634736E-4
17	0.0	1.2972399663028946E-5	0.0	0.00884717657018574	1.686411956193763E-4
18	0.0	1.2972399663028946E-5	0.0	0.011337877305487298	2.0755839460846314E-4
19	0.0	1.2972399663028946E-5	0.0	0.017331125949806674	2.0755839460846314E-4
20	0.0	1.2972399663028946E-5	0.0	0.02302600940187638	2.0755839460846314E-4
21	0.0	1.2972399663028946E-5	0.0	0.03562220947467749	2.205307942714921E-4
22	0.0	1.2972399663028946E-5	0.0	0.051993377849420014	2.205307942714921E-4
23	0.0	1.2972399663028946E-5	0.0	0.07254165891565786	3.113375919126947E-4
24	0.0	1.2972399663028946E-5	0.0	0.10503752007154538	3.2430999157572366E-4
25	0.0	1.2972399663028946E-5	0.0	0.12488529155597966	3.2430999157572366E-4
26	0.0	1.2972399663028946E-5	0.0	0.16246633337977454	3.2430999157572366E-4
27	0.0	1.2972399663028946E-5	0.0	0.24542482922484463	3.2430999157572366E-4
28	0.0	1.2972399663028946E-5	0.0	0.3844630088131889	3.2430999157572366E-4
29	0.0	1.2972399663028946E-5	0.0	0.5110606571246884	3.2430999157572366E-4
30	0.0	1.2972399663028946E-5	0.0	0.7015733185759315	3.2430999157572366E-4
31	0.0	1.2972399663028946E-5	0.0	1.0948835039593061	3.2430999157572366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1985	0.0	15.843829	5
AAAAGTA	4385	0.0	14.850628	2
AAAAAGT	4305	0.0	14.825785	1
AGTACTC	4620	0.0	14.655845	5
CAAGACG	2180	0.0	14.59633	4
TGGGCTG	6060	0.0	14.226073	36
TAGACCG	215	1.9826984E-10	13.767442	5
AAGTACT	4875	0.0	13.737435	4
GGTTATC	1565	0.0	13.59425	2
AAAGTAC	4805	0.0	13.591051	3
GTAATAC	370	0.0	13.5	3
GACGGAC	2330	0.0	13.418455	7
GGGCTGT	6800	0.0	13.385295	37
TCGTTAC	555	0.0	13.333332	23
CGAGCCG	2075	0.0	13.106024	15
ACGGACC	2355	0.0	13.040339	8
CGGACCA	2420	0.0	12.919422	9
TAGACGG	145	1.3742685E-5	12.758621	5
CTTATAC	9290	0.0	12.724973	37
CTAGCGG	1315	0.0	12.661597	29
>>END_MODULE