FastQCFastQC Report
Thu 9 Feb 2017
SRR2727181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991692
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC662491.1056809996241461No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG623631.0408245283636075No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA428540.7152236797218549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT383890.6407038279003661No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG334380.5580727447271989No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG301690.5035138655324739No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC293580.4899784568365663No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT263500.4397756092936686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT252700.42175065073438356No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG251530.41979794689046096No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT225090.3756701779731001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221130.3690610265013622No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT192120.3206439850379492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189830.31682202623232303No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA186040.31049660096012943No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC173940.2903019714631526No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG172860.2884994756072241No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166250.27746753337788393No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC164600.2747137202646598No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC135850.22673061298878516No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT133790.22329251904136593No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT133480.2227751359716087No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT127050.21204360971825656No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC124420.20765419851354175No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC122530.20449983076566686No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT122290.20409927613101608No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC113000.18859447381474215No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA110930.18513969009087916No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG105240.17564320729436694No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT101260.1690006762697415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC98830.16494506059390235No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96160.16048889028341243No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG95160.15881991263903417No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT95020.1585862557688212No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT88820.14823859437367606No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT86790.14485056975558824No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG85300.14236379306546465No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG83450.1392761844233649No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77400.1291788696748765No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT75940.12674216231408425No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75560.1261079508092205No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG73840.12323730926088992No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT70850.11824706610419895No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG68400.11415807087547224No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT67130.11203846926711186No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA66280.11061983826939036No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC63770.10643070438200095No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT63760.10641401460555716No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT61930.10335978551634496No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT60360.1007394906146711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT35200.018.2897721
AAAAGTA37100.017.6024272
CAAGACG16050.015.6760134
AAGACGG15550.015.4662375
CGAACGT6900.015.2826084
TATACTG5950.015.2352945
TATAACG2700.015.0740732
AGTACTC44200.014.9841635
TCGAACG7300.014.9520543
AAAGTAC44750.014.6759793
AAGTACT46100.014.6073744
TGGGCTG55350.014.43902536
TCGTTAC2750.014.12727423
TGTGCGA2500.014.05999910
AGTATCG1601.787248E-713.8750015
ACCGTCG11950.013.7782438
GGGCTGT61050.013.60606137
TCTAGCG10700.013.48598128
ATTCGAA7850.013.4331211
TATCACG2900.013.3965512
Produced by FastQC (version 0.11.5)