##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727181.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5991692
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208896084778722	31.0	31.0	33.0	30.0	34.0
2	31.312539596494613	31.0	31.0	34.0	30.0	34.0
3	31.377824828111994	31.0	31.0	34.0	28.0	34.0
4	35.18842423809502	35.0	35.0	37.0	33.0	37.0
5	35.05632899688435	35.0	35.0	37.0	32.0	37.0
6	35.123184402669565	37.0	35.0	37.0	32.0	37.0
7	34.93763514546475	36.0	35.0	37.0	32.0	37.0
8	34.89299783767257	36.0	35.0	37.0	32.0	37.0
9	36.51260812471669	38.0	35.0	39.0	32.0	39.0
10	36.313871273757066	38.0	35.0	39.0	32.0	39.0
11	36.42726027973401	38.0	35.0	39.0	32.0	39.0
12	36.31700861793296	38.0	35.0	39.0	32.0	39.0
13	36.402258493927924	38.0	35.0	39.0	32.0	39.0
14	37.3845110863509	39.0	36.0	41.0	32.0	41.0
15	37.26888331376179	39.0	36.0	41.0	32.0	41.0
16	37.19390032064398	39.0	36.0	40.0	31.0	41.0
17	37.168582263574294	39.0	36.0	40.0	31.0	41.0
18	37.16146240494338	39.0	36.0	40.0	31.0	41.0
19	37.178878186662466	39.0	36.0	40.0	31.0	41.0
20	37.10916365527467	39.0	36.0	40.0	31.0	41.0
21	36.9775555886384	39.0	36.0	40.0	31.0	41.0
22	36.96239059017052	39.0	36.0	40.0	31.0	41.0
23	36.748821868680835	39.0	35.0	40.0	30.0	41.0
24	36.63280355532294	38.0	35.0	40.0	30.0	41.0
25	36.556722041119606	38.0	35.0	40.0	30.0	41.0
26	36.21356121109029	38.0	35.0	40.0	30.0	41.0
27	36.343726780348526	38.0	35.0	40.0	30.0	41.0
28	36.196956051813075	38.0	35.0	40.0	30.0	41.0
29	36.10836104392549	38.0	35.0	40.0	29.0	41.0
30	36.17871496064885	38.0	35.0	40.0	30.0	41.0
31	36.08671390318461	38.0	35.0	40.0	30.0	41.0
32	35.82769524868768	38.0	34.0	40.0	29.0	41.0
33	35.76416878571195	38.0	34.0	40.0	28.0	41.0
34	35.73868600054876	38.0	34.0	40.0	28.0	41.0
35	35.46076367076278	38.0	34.0	40.0	27.0	41.0
36	35.306176452327655	38.0	34.0	40.0	26.0	41.0
37	35.058875189178615	38.0	34.0	40.0	25.0	41.0
38	34.89562664436023	38.0	33.0	40.0	24.0	41.0
39	34.80409023027218	38.0	33.0	40.0	24.0	41.0
40	34.648582403768415	38.0	33.0	40.0	24.0	41.0
41	34.48076686852395	38.0	33.0	40.0	23.0	41.0
42	34.30493957299541	38.0	33.0	40.0	22.0	41.0
43	33.84557450549861	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	14.0
14	19.0
15	73.0
16	188.0
17	446.0
18	803.0
19	1803.0
20	3452.0
21	6317.0
22	11378.0
23	18318.0
24	28746.0
25	43621.0
26	64059.0
27	90118.0
28	123491.0
29	160031.0
30	200736.0
31	243148.0
32	291266.0
33	346178.0
34	417429.0
35	498869.0
36	610995.0
37	781983.0
38	1016065.0
39	1032138.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.530959201507684	19.09045057723261	12.339402626169703	25.039187595090002
2	15.940171824586443	25.68176067795207	32.73532751683498	25.642739980626505
3	21.744425447770013	27.284012596108077	28.873964149025017	22.097597807096893
4	15.855621417122242	17.937070196532133	31.49157199669142	34.715736389654204
5	13.697766841152717	38.001269090600786	33.335475187976954	14.965488880269548
6	33.087615318010336	31.61349081361325	17.645700079376578	17.653193788999836
7	25.201228634582684	31.248401953905507	23.739487944306884	19.810881467204926
8	27.52891837564414	31.798346777504584	23.523822653100325	17.14891219375095
9	27.946897136902233	14.197809233184882	20.699778960600778	37.155514669312105
10	18.466169489352925	27.18011873774553	32.8586148954252	21.495096877476346
11	32.45360409046393	23.167829721554444	20.88942489033148	23.489141297650146
12	21.43224317938906	28.108320654666496	29.488949031425516	20.97048713451893
13	30.650440643477666	19.170377916621884	25.611096164489094	24.568085275411352
14	22.679303275268488	22.53890887582339	26.567921048011144	28.213866800896977
15	28.116465265571062	24.498155112111906	24.244220163519753	23.14115945879728
16	22.05328645063865	25.530267577171855	29.931511833385294	22.484934138804196
17	24.557253610499338	27.515216069183797	27.26597094777235	20.661559372544517
18	24.739021965748574	22.32053984083294	30.27248396613177	22.667954227286717
19	23.507249705091652	28.994414265619795	28.182239674536007	19.316096354752545
20	22.931368968898937	24.63579569844378	29.732603077728292	22.70023225492899
21	21.857932617364177	27.714291722605232	28.721336143446624	21.706439516583963
22	23.148836755961423	27.514264751926497	24.675667574367974	24.661230917744103
23	20.134613060884973	26.67975923996093	27.62932407072994	25.55630362842416
24	21.109846767824514	30.857460630486344	26.55241624569487	21.480276355994267
25	23.25691974821136	26.227165882358438	24.36991086991788	26.146003499512325
26	22.470514171956772	26.693561685079942	27.68904009084579	23.146884052117496
27	24.167931195395223	25.963684381640444	25.374818999374465	24.493565423589864
28	21.703669013694295	27.313386602649135	28.381849400803645	22.601094982852924
29	22.804793704349287	25.213395481610203	28.831722324845803	23.150088489194705
30	21.448048397681323	28.573347895719607	29.53386121983573	20.44474248676334
31	25.151409651897993	25.77861145065534	24.842481889923583	24.227497007523084
32	19.01155800398285	27.879570578728014	28.1409157880612	24.967955629227937
33	21.423197320556532	27.086589230554576	28.569425798255317	22.920787650633578
34	20.777336351735034	26.75014002722436	28.380080284500604	24.092443336539997
35	20.56976560210371	28.599634293618564	29.752413842367066	21.07818626191066
36	23.020058440921197	26.62878866270162	27.188997698813623	23.16215519756356
37	21.23892549884073	27.22189324818432	28.581459127071284	22.95772212590367
38	22.154710222087516	26.548494148230585	28.855405117619533	22.44139051206237
39	22.438519870514035	23.64807803872429	28.367629711273544	25.54577237948813
40	21.619969784828726	26.01382047007757	29.221094809279247	23.145114935814455
41	20.121728553470373	25.693994284085363	29.562968189953686	24.621308972490574
42	21.96079170958721	26.833271803690845	28.92982483078236	22.27611165593959
43	20.936974063419818	24.096065018028295	29.97730857994703	24.989652338604852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2024.0
1	2344.5
2	2665.0
3	9423.5
4	16182.0
5	16182.0
6	22619.0
7	29056.0
8	29499.0
9	29942.0
10	35233.0
11	40524.0
12	40524.0
13	57401.0
14	74278.0
15	95004.0
16	115730.0
17	104769.0
18	93808.0
19	93808.0
20	92173.0
21	90538.0
22	76003.5
23	61469.0
24	63191.5
25	64914.0
26	64914.0
27	68498.5
28	72083.0
29	75126.5
30	78170.0
31	84742.5
32	91315.0
33	91315.0
34	106680.0
35	122045.0
36	146015.0
37	169985.0
38	195542.0
39	221099.0
40	221099.0
41	240004.5
42	258910.0
43	292793.0
44	326676.0
45	390903.5
46	455131.0
47	455131.0
48	696926.5
49	938722.0
50	930547.5
51	922373.0
52	659466.0
53	396559.0
54	396559.0
55	350222.0
56	303885.0
57	289774.5
58	275664.0
59	255105.5
60	234547.0
61	234547.0
62	196886.0
63	159225.0
64	138360.0
65	117495.0
66	99275.0
67	81055.0
68	81055.0
69	67907.5
70	54760.0
71	46828.0
72	38896.0
73	28994.0
74	19092.0
75	19092.0
76	15070.0
77	11048.0
78	9655.5
79	8263.0
80	6796.0
81	5329.0
82	5329.0
83	4482.0
84	3635.0
85	3242.0
86	2849.0
87	1908.5
88	968.0
89	968.0
90	691.5
91	415.0
92	294.5
93	174.0
94	173.0
95	172.0
96	172.0
97	90.5
98	9.0
99	11.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5991692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.39436775978495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9581674707334	28.654631526173972
2	9.246409252036372	6.5454161904810695
3	3.3189950504655257	3.524211942272485
4	1.6615603631944773	2.3523951419994877
5	0.9411213677884398	1.6655197899047933
6	0.67110907065024	1.4252088752113254
7	0.4791291886572417	1.1870932295447258
8	0.37903184012250984	1.0732473873571302
9	0.28149700516512655	0.8967067671683315
>10	1.7695778922680334	11.677498667393065
>50	0.14206933800256036	3.4827527491963775
>100	0.11472660139736374	8.561378593595844
>500	0.019817990548687815	4.9038513356298985
>1k	0.013853352044164522	8.782928356905567
>5k	0.0014911594214204744	3.6598925706499656
>10k+	0.001443057504600459	11.607266876516082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	66249	1.1056809996241461	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	62363	1.0408245283636075	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	42854	0.7152236797218549	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38389	0.6407038279003661	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	33438	0.5580727447271989	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	30169	0.5035138655324739	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	29358	0.4899784568365663	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	26350	0.4397756092936686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25270	0.42175065073438356	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	25153	0.41979794689046096	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	22509	0.3756701779731001	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22113	0.3690610265013622	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	19212	0.3206439850379492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18983	0.31682202623232303	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	18604	0.31049660096012943	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	17394	0.2903019714631526	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	17286	0.2884994756072241	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	16625	0.27746753337788393	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	16460	0.2747137202646598	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	13585	0.22673061298878516	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	13379	0.22329251904136593	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	13348	0.2227751359716087	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	12705	0.21204360971825656	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	12442	0.20765419851354175	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	12253	0.20449983076566686	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	12229	0.20409927613101608	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	11300	0.18859447381474215	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	11093	0.18513969009087916	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	10524	0.17564320729436694	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	10126	0.1690006762697415	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	9883	0.16494506059390235	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9616	0.16048889028341243	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	9516	0.15881991263903417	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	9502	0.1585862557688212	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	8882	0.14823859437367606	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	8679	0.14485056975558824	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	8530	0.14236379306546465	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8345	0.1392761844233649	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	7740	0.1291788696748765	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	7594	0.12674216231408425	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7556	0.1261079508092205	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	7384	0.12323730926088992	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	7085	0.11824706610419895	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	6840	0.11415807087547224	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	6713	0.11203846926711186	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	6628	0.11061983826939036	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	6377	0.10643070438200095	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	6376	0.10641401460555716	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	6193	0.10335978551634496	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	6036	0.1007394906146711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.337955288756498E-5
5	0.0	0.0	0.0	0.0	3.337955288756498E-5
6	0.0	0.0	0.0	0.0	8.344888221891246E-5
7	0.0	0.0	0.0	0.0	8.344888221891246E-5
8	0.0	0.0	0.0	0.0	1.0013865866269495E-4
9	0.0	0.0	0.0	3.337955288756498E-5	1.1682843510647743E-4
10	0.0	0.0	0.0	1.6689776443782491E-4	1.1682843510647743E-4
11	0.0	0.0	0.0	3.504853053194323E-4	1.1682843510647743E-4
12	0.0	0.0	0.0	8.84558151520472E-4	1.3351821155025993E-4
13	0.0	0.0	0.0	0.0012851127861712517	1.3351821155025993E-4
14	0.0	1.668977644378249E-5	0.0	0.0024033278079046787	1.835875408816074E-4
15	0.0	1.668977644378249E-5	0.0	0.004005546346507798	2.002773173253899E-4
16	0.0	1.668977644378249E-5	0.0	0.008495096209885289	2.002773173253899E-4
17	0.0	1.668977644378249E-5	0.0	0.012700919873718476	2.002773173253899E-4
18	0.0	1.668977644378249E-5	0.0	0.01613901382113767	2.002773173253899E-4
19	0.0	1.668977644378249E-5	0.0	0.024233555396372175	2.002773173253899E-4
20	0.0	1.668977644378249E-5	0.0	0.031510297925861346	2.002773173253899E-4
21	0.0	1.668977644378249E-5	0.0	0.047081859347910406	2.002773173253899E-4
22	0.0	1.668977644378249E-5	0.0	0.06864505051327738	2.1696709376917237E-4
23	0.0	1.668977644378249E-5	0.0	0.09611642253974337	2.8372619954430235E-4
24	1.668977644378249E-5	1.668977644378249E-5	0.0	0.13662250996880346	2.8372619954430235E-4
25	1.668977644378249E-5	1.668977644378249E-5	0.0	0.1615570359758145	3.0041597598808484E-4
26	1.668977644378249E-5	1.668977644378249E-5	0.0	0.20858882599439357	3.1710575243186734E-4
27	1.668977644378249E-5	1.668977644378249E-5	0.0	0.3134340016142352	3.3379552887564983E-4
28	1.668977644378249E-5	1.668977644378249E-5	0.0	0.49430110893550605	3.3379552887564983E-4
29	1.668977644378249E-5	1.668977644378249E-5	0.0	0.6514687337066057	3.3379552887564983E-4
30	1.668977644378249E-5	1.668977644378249E-5	0.0	0.8864107167057319	3.3379552887564983E-4
31	1.668977644378249E-5	1.668977644378249E-5	0.0	1.347866345599874	3.504853053194323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGT	3520	0.0	18.289772	1
AAAAGTA	3710	0.0	17.602427	2
CAAGACG	1605	0.0	15.676013	4
AAGACGG	1555	0.0	15.466237	5
CGAACGT	690	0.0	15.282608	4
TATACTG	595	0.0	15.235294	5
TATAACG	270	0.0	15.074073	2
AGTACTC	4420	0.0	14.984163	5
TCGAACG	730	0.0	14.952054	3
AAAGTAC	4475	0.0	14.675979	3
AAGTACT	4610	0.0	14.607374	4
TGGGCTG	5535	0.0	14.439025	36
TCGTTAC	275	0.0	14.127274	23
TGTGCGA	250	0.0	14.059999	10
AGTATCG	160	1.787248E-7	13.875001	5
ACCGTCG	1195	0.0	13.778243	8
GGGCTGT	6105	0.0	13.606061	37
TCTAGCG	1070	0.0	13.485981	28
ATTCGAA	785	0.0	13.433121	1
TATCACG	290	0.0	13.396551	2
>>END_MODULE