##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5346631
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211409577358154	31.0	31.0	33.0	30.0	34.0
2	31.296181464552163	31.0	31.0	34.0	30.0	34.0
3	31.36969317688092	31.0	31.0	34.0	28.0	34.0
4	35.17072133835307	35.0	35.0	37.0	33.0	37.0
5	35.0411565713063	35.0	35.0	37.0	32.0	37.0
6	35.12438225117836	37.0	35.0	37.0	32.0	37.0
7	34.93683012723339	36.0	35.0	37.0	32.0	37.0
8	34.891331756390144	36.0	35.0	37.0	32.0	37.0
9	36.524544895654856	38.0	35.0	39.0	32.0	39.0
10	36.30987494742016	38.0	35.0	39.0	32.0	39.0
11	36.43588326929612	38.0	35.0	39.0	32.0	39.0
12	36.3221488447585	38.0	35.0	39.0	32.0	39.0
13	36.40591710929743	38.0	35.0	39.0	32.0	39.0
14	37.386363487586856	39.0	36.0	41.0	32.0	41.0
15	37.25711331116735	39.0	36.0	41.0	32.0	41.0
16	37.16160120270129	39.0	36.0	40.0	31.0	41.0
17	37.15707779347406	39.0	36.0	40.0	31.0	41.0
18	37.1328144770043	39.0	36.0	40.0	31.0	41.0
19	37.13281241963397	39.0	36.0	40.0	31.0	41.0
20	37.089490933636526	39.0	36.0	40.0	31.0	41.0
21	36.94494570506175	39.0	36.0	40.0	31.0	41.0
22	36.94729335164518	39.0	36.0	40.0	31.0	41.0
23	36.728720197821765	39.0	35.0	40.0	30.0	41.0
24	36.62203544624643	38.0	35.0	40.0	30.0	41.0
25	36.5377556446293	38.0	35.0	40.0	30.0	41.0
26	36.17597567514945	38.0	35.0	40.0	29.0	41.0
27	36.304972795018024	38.0	35.0	40.0	30.0	41.0
28	36.157990143699834	38.0	35.0	40.0	30.0	41.0
29	36.07276301656127	38.0	35.0	40.0	29.0	41.0
30	36.13964812608164	38.0	35.0	40.0	30.0	41.0
31	36.04449231675049	38.0	35.0	40.0	29.0	41.0
32	35.745858092694256	38.0	34.0	40.0	28.0	41.0
33	35.7055961034154	38.0	34.0	40.0	28.0	41.0
34	35.67097879019517	38.0	34.0	40.0	27.0	41.0
35	35.394828257270795	38.0	34.0	40.0	26.0	41.0
36	35.25878670138261	38.0	34.0	40.0	25.0	41.0
37	35.000116334940635	38.0	34.0	40.0	25.0	41.0
38	34.845446225856996	38.0	33.0	40.0	24.0	41.0
39	34.76765761467362	38.0	33.0	40.0	24.0	41.0
40	34.60140488468346	38.0	33.0	40.0	24.0	41.0
41	34.43767804436102	38.0	33.0	40.0	23.0	41.0
42	34.25566305211637	38.0	33.0	40.0	22.0	41.0
43	33.77933094690844	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	5.0
14	21.0
15	62.0
16	194.0
17	376.0
18	806.0
19	1634.0
20	3178.0
21	5934.0
22	10280.0
23	16909.0
24	26257.0
25	40050.0
26	57824.0
27	81816.0
28	110829.0
29	145261.0
30	180222.0
31	218879.0
32	259243.0
33	310190.0
34	371416.0
35	445311.0
36	546312.0
37	700623.0
38	919049.0
39	893941.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.05987620989741	18.742082631099848	12.034830905667512	25.163210253335233
2	15.295164375473078	26.060859632916504	32.45765417512448	26.18632181648593
3	22.16184359833323	27.705110002915855	28.521119187017018	21.611927211733896
4	15.90781185385713	18.21202921989567	30.86418344561276	35.015975480634445
5	13.487465284213554	38.03877619383122	33.45830299491399	15.015455527041233
6	33.08965963800382	30.695497781687198	17.988168624316884	18.2266739559921
7	24.78254063166132	31.01119190757694	24.029393462911504	20.176873997850233
8	27.734137627975446	31.387092170752013	24.1102481169918	16.768522084280736
9	28.11430225874948	14.159552061849789	20.73786277751354	36.98828290188719
10	18.684719405547156	27.174570304178463	32.859084533793336	21.28162575648104
11	32.191542674256	23.083264956942045	20.72686519791622	23.99832717088574
12	21.559314641313378	28.286766002740794	29.54999138709965	20.603927968846175
13	30.964508304388318	18.894253222262765	25.35404070338873	24.787197769960187
14	22.78189760991548	22.584782828663506	26.708014822792148	27.92530473862887
15	28.884114127195236	23.455050479451454	24.50099885329659	23.15983654005672
16	21.868369072038075	25.134126518175652	30.901702399136955	22.095802010649322
17	25.20228158629238	27.18953299750815	27.534479188857432	20.07370622734204
18	25.38104836484882	21.25108315872182	30.83004980145441	22.53781867497495
19	23.84215405925713	29.269721437667943	28.196279114829508	18.69184538824542
20	22.988494998065136	24.1653856419117	30.220189124703012	22.625930235320148
21	21.475486151933808	27.713881881880386	29.10973283924026	21.700899126945547
22	23.08401309160853	27.571642778415047	24.201333512636275	25.143010617340156
23	19.645754494746317	26.530014882268855	27.643744256897474	26.18048636608735
24	20.917882681636343	31.35888749382555	26.415550278296745	21.307679546241364
25	23.37559857787081	25.841712285736566	23.87239740314976	26.910291733242858
26	22.620655885921433	26.20188301754881	27.718052732646036	23.459408363883725
27	24.53591803885475	25.343884775291208	25.05918212796058	25.061015057893464
28	21.87633670623613	26.963839471996476	28.466449246263675	22.693374575503714
29	23.027416704088985	24.417806278383527	29.071091683716343	23.48368533381114
30	21.39934474625236	28.476193700294633	30.039552009480364	20.084909543972643
31	26.02663247192484	25.009767833239287	24.263391283221154	24.700208411614717
32	18.46343987456774	27.629997282400826	28.39030784058223	25.516255002449206
33	21.563971779612245	26.723763057521644	28.82164488254379	22.89062028032232
34	20.439506672519574	26.31849850868706	28.61598266272724	24.62601215606613
35	20.339499770977277	28.625166015758335	30.16260145875038	20.87273275451401
36	23.493261457542143	26.173042426155835	26.99215636912291	23.34153974717911
37	21.29860841341024	27.07104716970369	28.569822753805152	23.06052166308092
38	22.61798130448875	26.2346139092075	28.79271077431751	22.35469401198624
39	23.009293141793403	22.79184780097972	28.134445784644573	26.064413272582303
40	21.996300100006902	25.682920702775263	28.949295360012687	23.371483837205147
41	20.316924807416108	25.314277345865086	29.326654485787408	25.042143360931394
42	22.620281818588193	26.632284891177267	28.655764723617544	22.091668566616995
43	21.53786187975194	23.267511821930484	29.818777469400825	25.37584882891675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1486.0
1	1848.5
2	2211.0
3	9177.5
4	16144.0
5	16144.0
6	22045.0
7	27946.0
8	28150.0
9	28354.0
10	32974.0
11	37594.0
12	37594.0
13	51582.5
14	65571.0
15	82958.5
16	100346.0
17	89988.5
18	79631.0
19	79631.0
20	78023.0
21	76415.0
22	63487.0
23	50559.0
24	51134.0
25	51709.0
26	51709.0
27	53956.5
28	56204.0
29	58092.5
30	59981.0
31	64851.0
32	69721.0
33	69721.0
34	81907.5
35	94094.0
36	114404.5
37	134715.0
38	156018.0
39	177321.0
40	177321.0
41	194174.5
42	211028.0
43	243126.0
44	275224.0
45	340272.0
46	405320.0
47	405320.0
48	670334.0
49	935348.0
50	928475.5
51	921603.0
52	635476.0
53	349349.0
54	349349.0
55	302145.5
56	254942.0
57	243891.5
58	232841.0
59	216586.5
60	200332.0
61	200332.0
62	167166.0
63	134000.0
64	116304.0
65	98608.0
66	83615.5
67	68623.0
68	68623.0
69	57868.5
70	47114.0
71	40668.0
72	34222.0
73	25764.5
74	17307.0
75	17307.0
76	13641.0
77	9975.0
78	8714.0
79	7453.0
80	6361.5
81	5270.0
82	5270.0
83	4373.5
84	3477.0
85	3119.0
86	2761.0
87	1892.0
88	1023.0
89	1023.0
90	725.5
91	428.0
92	310.5
93	193.0
94	179.5
95	166.0
96	166.0
97	86.0
98	6.0
99	11.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5346631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.7103340565556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4612494418443	28.622575148473757
2	8.753759614991035	6.076918409742488
3	2.997826167053875	3.1216664310577102
4	1.5120195672827992	2.0993081712173844
5	0.8910742553430069	1.5464742536076148
6	0.5983040761211255	1.2460400609737883
7	0.4372493569426263	1.0623949871844875
8	0.3190399244314919	0.8859185883516271
9	0.2484893893892219	0.7762634743688474
>10	1.4934801856134752	9.570833842504548
>50	0.1246373897976476	3.0230825110393313
>100	0.1234950031539964	9.203455689385713
>500	0.021312402879251537	5.15846360602261
>1k	0.01470390584368676	9.251614559834145
>5k	0.0015970534436963147	4.006577939167871
>10k+	0.0017622658689062781	14.3484123270682
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	75184	1.406193919124024	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	68950	1.2895971313524348	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	48469	0.9065334787457746	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	36202	0.6770992799016802	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	34230	0.6402162408440006	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	33682	0.6299667959131647	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32762	0.6127596985840242	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	28446	0.5320359680703606	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	27522	0.5147540572745716	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	23717	0.44358774712524573	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	21815	0.408013943733914	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21073	0.3941360456706289	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	20600	0.3852893532394512	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	20219	0.37816337054118754	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	19959	0.37330049520903913	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	18762	0.35091256531449433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18217	0.3407192304836448	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	18030	0.33722170091783027	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16845	0.3150582114232308	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	14587	0.27282600950018804	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	14576	0.2726202724669049	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	14307	0.2675890668347975	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	14128	0.2642411642022799	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	13746	0.25709647813735415	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	13647	0.25524484483780535	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	12996	0.24306895314077218	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	12708	0.2376823835420847	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	12219	0.2285364372443133	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	11489	0.2148829795809735	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	10836	0.20266968115061615	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	10371	0.19397261565273535	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	10332	0.19324318435291307	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	9983	0.18671570938783694	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	9976	0.18658478582120216	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	9915	0.1854438804548135	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	9496	0.17760716982338973	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9163	0.17137894872490733	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9017	0.1686482571922394	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	8547	0.15985767486104802	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	8405	0.1572017967950285	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	7926	0.14824288416387815	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7912	0.1479810370306086	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	7694	0.1439037030982688	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	7519	0.14063061393239967	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	7284	0.136235322766804	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	6985	0.13064301613483334	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	6868	0.12845472223536653	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	6709	0.12548088693609116	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	6707	0.12544348020276694	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	6702	0.1253499633694564	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	6527	0.12207687420358727	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	6415	0.11998209713743103	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	6271	0.1172888123380873	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	6098	0.11405312990554238	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTC	5532	0.10346702437478852	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	5426	0.10148446750860496	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5411	0.1012039170086733	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	5409	0.10116651027534909	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	5366	0.1003622655088784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8703366662109278E-5
2	0.0	1.8703366662109278E-5	0.0	0.0	1.8703366662109278E-5
3	0.0	1.8703366662109278E-5	0.0	0.0	1.8703366662109278E-5
4	0.0	1.8703366662109278E-5	0.0	0.0	3.7406733324218556E-5
5	0.0	1.8703366662109278E-5	0.0	0.0	3.7406733324218556E-5
6	0.0	1.8703366662109278E-5	0.0	0.0	9.35168333105464E-5
7	0.0	1.8703366662109278E-5	0.0	0.0	9.35168333105464E-5
8	0.0	1.8703366662109278E-5	0.0	0.0	1.1222019997265568E-4
9	0.0	1.8703366662109278E-5	0.0	1.8703366662109278E-5	1.4962693329687423E-4
10	0.0	1.8703366662109278E-5	0.0	2.0573703328320207E-4	1.6833029995898352E-4
11	0.0	1.8703366662109278E-5	0.0	4.67584166552732E-4	1.6833029995898352E-4
12	0.0	1.8703366662109278E-5	0.0	0.0011970154663749938	1.6833029995898352E-4
13	0.0	3.7406733324218556E-5	0.0	0.001664599632927726	1.6833029995898352E-4
14	0.0	3.7406733324218556E-5	0.0	0.0027868016326542825	2.0573703328320207E-4
15	0.0	3.7406733324218556E-5	0.0	0.004376587798933572	2.9925386659374845E-4
16	0.0	3.7406733324218556E-5	0.0	0.008678362131218706	3.740673332421856E-4
17	0.0	3.7406733324218556E-5	0.0	0.014195855296540943	3.740673332421856E-4
18	0.0	3.7406733324218556E-5	0.0	0.018348002695529202	4.4888079989062273E-4
19	0.0	3.7406733324218556E-5	0.0	0.026914144626775254	4.4888079989062273E-4
20	0.0	3.7406733324218556E-5	0.0	0.03448900812492951	4.67584166552732E-4
21	0.0	3.7406733324218556E-5	0.0	0.05298663775375559	4.862875332148413E-4
22	0.0	3.7406733324218556E-5	0.0	0.07572993161488048	4.862875332148413E-4
23	0.0	3.7406733324218556E-5	0.0	0.10713288424056196	5.611009998632784E-4
24	0.0	3.7406733324218556E-5	0.0	0.1543962917957121	5.611009998632784E-4
25	0.0	3.7406733324218556E-5	0.0	0.18248874852220023	5.611009998632784E-4
26	0.0	3.7406733324218556E-5	0.0	0.23485817517610622	5.611009998632784E-4
27	0.0	3.7406733324218556E-5	0.0	0.3302827518861878	5.798043665253877E-4
28	0.0	3.7406733324218556E-5	0.0	0.5028587160774701	5.798043665253877E-4
29	0.0	3.7406733324218556E-5	0.0	0.6480529514754244	5.798043665253877E-4
30	0.0	3.7406733324218556E-5	0.0	0.8694821093881362	5.798043665253877E-4
31	0.0	3.7406733324218556E-5	0.0	1.2972468083172375	5.798043665253877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	3715	0.0	19.222073	2
AAAAAGT	3710	0.0	19.148249	1
TACAACG	185	1.8189894E-12	17.0	2
TATAACG	255	0.0	15.960784	2
CAAGACG	1315	0.0	15.756653	4
AAGACGG	1355	0.0	15.154982	5
TGGGCTG	5370	0.0	14.882682	36
GGGCTGT	5600	0.0	14.568749	37
AAAGTAC	4915	0.0	14.528994	3
AGTACTC	5100	0.0	14.473528	5
TATACTG	385	0.0	14.415584	5
ATACTAC	360	0.0	14.388888	1
AAGTACT	5220	0.0	14.282568	4
GTATAAC	405	0.0	14.160494	1
CACTCTA	1110	0.0	13.833333	9
TAGAGTG	845	0.0	13.7929	5
GGGTTAG	270	0.0	13.703704	1
TAGGTCG	95	0.0012461144	13.631579	5
ACTCTAA	1135	0.0	13.365639	10
TATACCG	125	4.8508526E-5	13.32	5
>>END_MODULE