FastQCFastQC Report
Thu 9 Feb 2017
SRR2727179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9348478
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1118911.1968900178189432No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1093271.1694630933505967No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA726130.7767360633463544No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG633580.6777359908211796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT498880.5336483650065819No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG494530.5289952011439722No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT461620.4937916097144369No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC444490.4754677713313333No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG429030.45893031999433487No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT353500.3781364196396461No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC338260.3618343007278832No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT331730.35484920646975904No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC330090.35309491020891315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT328610.3515117648027839No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG311320.3330167755649636No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT308570.33007512024952085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308060.3295295769001114No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA294740.31528126824494856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275230.2944115608979344No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT246500.26367928554787207No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC242840.2597642097462282No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC235270.2516666349324457No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT228350.24426436046594965No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT226800.24260633656088187No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC213960.228871480469869No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC212900.22773760605737103No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA206530.22092366265396354No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT199190.21307211719383629No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG185650.19858847611343794No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG183180.19594633479374934No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT182370.19507988359174616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC178980.1914536248574367No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT174880.187067884205322No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT173850.1859661005780834No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT170420.18229705413009475No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG157800.16879753046431728No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC151960.1625505242671588No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG147000.15724484777094197No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT135050.14446201830929056No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT127120.13597935407239553No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126680.1355086892219247No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG126090.13487757044515694No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG124860.13356184824952255No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT124760.13345487896532462No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT123090.13166849191921937No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA118020.12624514921038482No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC113350.12124968363834197No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC108260.11580494707266788No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT106820.11426458938021782No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC105590.11294886718458341No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG105250.1125851716183105No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT104720.11201823441206152No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG103110.11029602893647501No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94200.10076506571444037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT55850.016.4297241
GTATACG1557.228664E-915.51612951
TGGGCTG85000.015.47470636
AAAAGTA59850.015.4553042
TATAACG3600.015.4166662
GGGCTGT89950.015.11673237
AAGACGG26850.014.8826825
ACGAACG12600.014.82936615
CGAACGA12400.014.77016216
CAAGACG27700.014.5595664
AAGTACT68400.014.1454684
AGTACTC69100.014.0557165
TATACCG2800.013.8750015
AAAGTAC67550.013.8031083
AGGTTAT20450.013.5696821
CTTATAC93800.013.49040537
AACGAAC14000.013.47857214
TAACGAA14150.013.33568913
GGTTATC21600.013.0185182
CGAGCCG27350.012.98720315
Produced by FastQC (version 0.11.5)