##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9348478
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18128886862653	31.0	31.0	33.0	30.0	34.0
2	31.280985953007537	31.0	31.0	34.0	30.0	34.0
3	31.34034545516393	31.0	31.0	34.0	28.0	34.0
4	35.15770920143365	35.0	35.0	37.0	33.0	37.0
5	35.013044048453665	35.0	35.0	37.0	32.0	37.0
6	35.0833438341514	36.0	35.0	37.0	32.0	37.0
7	34.89054999113225	36.0	35.0	37.0	32.0	37.0
8	34.83800239996286	36.0	35.0	37.0	32.0	37.0
9	36.46170563807285	38.0	35.0	39.0	32.0	39.0
10	36.24879354692818	38.0	35.0	39.0	32.0	39.0
11	36.37052309477543	38.0	35.0	39.0	32.0	39.0
12	36.25675056410252	38.0	35.0	39.0	32.0	39.0
13	36.34802574280006	38.0	35.0	39.0	32.0	39.0
14	37.31850842457992	39.0	36.0	41.0	32.0	41.0
15	37.1953198156962	39.0	36.0	41.0	31.0	41.0
16	37.1061218735285	39.0	36.0	40.0	31.0	41.0
17	37.078288786688056	39.0	36.0	40.0	31.0	41.0
18	37.05836736204546	39.0	36.0	40.0	31.0	41.0
19	37.07250977110927	39.0	36.0	40.0	31.0	41.0
20	37.00848501756115	39.0	36.0	40.0	31.0	41.0
21	36.86837857456583	39.0	36.0	40.0	31.0	41.0
22	36.86985496462633	39.0	36.0	40.0	31.0	41.0
23	36.6558824869674	39.0	35.0	40.0	30.0	41.0
24	36.5426020150018	38.0	35.0	40.0	30.0	41.0
25	36.453268007904605	38.0	35.0	40.0	30.0	41.0
26	36.092418145499195	38.0	35.0	40.0	29.0	41.0
27	36.215463308572794	38.0	35.0	40.0	30.0	41.0
28	36.068689577062706	38.0	35.0	40.0	29.0	41.0
29	35.9786001528805	38.0	35.0	40.0	29.0	41.0
30	36.05969912963372	38.0	35.0	40.0	29.0	41.0
31	35.98521641704671	38.0	35.0	40.0	29.0	41.0
32	35.717623660236455	38.0	34.0	40.0	27.0	41.0
33	35.673158561211785	38.0	34.0	40.0	27.0	41.0
34	35.65780996649936	38.0	34.0	40.0	27.0	41.0
35	35.38594464253967	38.0	34.0	40.0	26.0	41.0
36	35.25973543500878	38.0	34.0	40.0	25.0	41.0
37	35.01591724342722	38.0	33.0	40.0	25.0	41.0
38	34.869968886913995	38.0	33.0	40.0	24.0	41.0
39	34.79614638874906	38.0	33.0	40.0	24.0	41.0
40	34.635422792886715	38.0	33.0	40.0	24.0	41.0
41	34.47877440584446	38.0	33.0	40.0	23.0	41.0
42	34.29757014992173	38.0	33.0	40.0	22.0	41.0
43	33.83180556235999	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	8.0
12	8.0
13	11.0
14	39.0
15	117.0
16	350.0
17	727.0
18	1611.0
19	3238.0
20	6215.0
21	11183.0
22	18957.0
23	30969.0
24	48714.0
25	73121.0
26	104017.0
27	145176.0
28	194544.0
29	254254.0
30	316804.0
31	385333.0
32	460803.0
33	547954.0
34	658590.0
35	784321.0
36	956667.0
37	1212894.0
38	1581057.0
39	1550795.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.884884576933274	19.218711323918182	12.360354273711721	25.536049825436823
2	15.96238446515037	25.636868375793366	32.268044060220284	26.13270309883598
3	22.025499765844238	26.770143760299803	28.948626717632536	22.25572975622342
4	16.23527380606768	18.098881978435422	31.05621043339889	34.609633782098
5	13.894122658255172	37.498638815858584	33.54197335651857	15.065265169367677
6	33.638983800357664	31.37822006961989	17.290868096389595	17.691928033632852
7	25.02162384080061	31.217316872329377	23.62082897344359	20.14023031342642
8	27.62712818065144	31.5574471052935	23.44814845796289	17.36727625609217
9	27.814345821854637	14.235771855054907	20.493945645483684	37.45593667760677
10	18.490870920378697	27.11330122400673	32.842950478142	21.55287737747257
11	32.85892099227276	22.847355473265274	20.33720355334847	23.9565199811135
12	21.523878004526512	27.96865971123856	29.183991233653224	21.32347105058171
13	31.115343053703505	18.829204069368295	25.121725696953025	24.93372717997518
14	22.74838749152536	22.7265229698353	25.98417624772717	28.54091329091217
15	28.76388006689431	24.12052528764575	23.822391195657733	23.293203449802203
16	22.54715687409223	25.261780580753356	29.56364661712848	22.627415928025933
17	24.898106408337274	27.426400318854043	26.88314611212649	20.792347160682198
18	25.283131649879266	21.76585322231063	30.030107574730348	22.920907553079765
19	23.75767477871799	29.23697312011645	27.526812385930626	19.47853971523493
20	23.254074085642603	24.15787896168767	29.109808035061963	23.47823891760776
21	22.091981175973245	27.78702586667049	27.827695588522538	22.293297368833727
22	23.30624300554593	27.373792824885506	24.140838754714938	25.179125414853626
23	20.198860178095302	26.008533153739037	27.318660855809895	26.473945812355765
24	21.524776546513774	30.77097683708514	25.907179756961508	21.797066859439578
25	23.844844048410874	25.552972366196936	23.60650578628949	26.995677799102697
26	22.873135070756973	26.313844884696742	27.181825747463918	23.63119429708237
27	24.84782014783583	25.49051300115377	24.747803867110775	24.913862983899627
28	22.051771422043245	26.670266539644206	28.08043191629696	23.197530122015582
29	23.277115269458836	24.713284879100105	28.342741995007103	23.666857856433957
30	21.760034093250262	28.262236911719747	29.114963954560302	20.86276504046969
31	25.790112572335307	25.446163535925315	23.786813211733502	24.97691068000588
32	19.241720416949153	27.532706393489935	27.586971911363538	25.63860127819737
33	22.05199605754006	26.52009236155875	28.1065538154981	23.321357765403093
34	21.31075240269058	26.301853627938154	27.733284498289457	24.654109471081817
35	21.20352639221058	28.236617768154343	29.382344377341425	21.177511462293648
36	23.785283550969474	26.392157097658036	26.317246507934232	23.505312843438258
37	21.622396715272796	26.96521294696313	28.074152819314545	23.338237518449528
38	22.922512092342732	26.033563966241353	28.256225237947824	22.78769870346809
39	23.07122079123468	22.955287481020974	27.772360377806955	26.20113134993739
40	22.34726337271158	25.59014419245571	28.709197368812333	23.353395066020372
41	20.830160802646162	25.052099389868598	28.914824423825998	25.202915383659242
42	22.980853139944276	26.239490535250766	28.44866298021988	22.330993344585075
43	21.87259787101173	23.186758315096853	29.53972828518182	25.400915528709593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2890.0
1	3311.0
2	3732.0
3	12040.0
4	20348.0
5	20348.0
6	29153.5
7	37959.0
8	38261.5
9	38564.0
10	45801.0
11	53038.0
12	53038.0
13	76878.5
14	100719.0
15	128205.5
16	155692.0
17	141761.0
18	127830.0
19	127830.0
20	128233.0
21	128636.0
22	107945.5
23	87255.0
24	89709.5
25	92164.0
26	92164.0
27	97147.0
28	102130.0
29	106180.0
30	110230.0
31	120190.5
32	130151.0
33	130151.0
34	154641.0
35	179131.0
36	217581.0
37	256031.0
38	293709.5
39	331388.0
40	331388.0
41	359287.5
42	387187.0
43	433574.5
44	479962.0
45	584483.5
46	689005.0
47	689005.0
48	1125980.0
49	1562955.0
50	1537722.0
51	1512489.0
52	1063353.5
53	614218.0
54	614218.0
55	543355.5
56	472493.0
57	457155.0
58	441817.0
59	413302.5
60	384788.0
61	384788.0
62	319290.5
63	253793.0
64	221399.5
65	189006.0
66	161946.5
67	134887.0
68	134887.0
69	115303.5
70	95720.0
71	83504.0
72	71288.0
73	53478.0
74	35668.0
75	35668.0
76	28219.5
77	20771.0
78	18405.5
79	16040.0
80	13446.5
81	10853.0
82	10853.0
83	9255.0
84	7657.0
85	6894.0
86	6131.0
87	4117.5
88	2104.0
89	2104.0
90	1536.5
91	969.0
92	681.5
93	394.0
94	374.0
95	354.0
96	354.0
97	189.0
98	24.0
99	20.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9348478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.364664698430573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.15850314162336	25.141445737651097
2	9.587114320832232	6.013932521968498
3	3.5089338643544528	3.30169602313337
4	1.7033151935906505	2.1369563969085243
5	1.0044392741538846	1.5751950521885782
6	0.6865818208922932	1.2920645160194815
7	0.48965581884940146	1.0750523403092853
8	0.37266256310598295	0.9350749069981503
9	0.30007579848157995	0.8470599123139719
>10	1.8707741792385861	11.062530825806489
>50	0.1575134355622452	3.416679639413991
>100	0.11665222005589786	7.759127933499515
>500	0.021296720569757392	4.679624994430572
>1k	0.01903097703093736	11.534932640751707
>5k	0.001603342386990929	3.3203066319594186
>10k+	0.0018473292719678098	15.90831992664733
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	111891	1.1968900178189432	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	109327	1.1694630933505967	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	72613	0.7767360633463544	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	63358	0.6777359908211796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49888	0.5336483650065819	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	49453	0.5289952011439722	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	46162	0.4937916097144369	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	44449	0.4754677713313333	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	42903	0.45893031999433487	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	35350	0.3781364196396461	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	33826	0.3618343007278832	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	33173	0.35484920646975904	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	33009	0.35309491020891315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32861	0.3515117648027839	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	31132	0.3330167755649636	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	30857	0.33007512024952085	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30806	0.3295295769001114	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	29474	0.31528126824494856	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27523	0.2944115608979344	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	24650	0.26367928554787207	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	24284	0.2597642097462282	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	23527	0.2516666349324457	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	22835	0.24426436046594965	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	22680	0.24260633656088187	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	21396	0.228871480469869	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	21290	0.22773760605737103	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	20653	0.22092366265396354	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	19919	0.21307211719383629	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	18565	0.19858847611343794	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	18318	0.19594633479374934	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	18237	0.19507988359174616	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	17898	0.1914536248574367	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	17488	0.187067884205322	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	17385	0.1859661005780834	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	17042	0.18229705413009475	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	15780	0.16879753046431728	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	15196	0.1625505242671588	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	14700	0.15724484777094197	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	13505	0.14446201830929056	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	12712	0.13597935407239553	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12668	0.1355086892219247	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12609	0.13487757044515694	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	12486	0.13356184824952255	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	12476	0.13345487896532462	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	12309	0.13166849191921937	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	11802	0.12624514921038482	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	11335	0.12124968363834197	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	10826	0.11580494707266788	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	10682	0.11426458938021782	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	10559	0.11294886718458341	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	10525	0.1125851716183105	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	10472	0.11201823441206152	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	10311	0.11029602893647501	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9420	0.10076506571444037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.278771367916788E-5
2	1.069692841979197E-5	0.0	0.0	0.0	4.278771367916788E-5
3	1.069692841979197E-5	0.0	0.0	0.0	4.278771367916788E-5
4	1.069692841979197E-5	0.0	0.0	0.0	4.278771367916788E-5
5	1.069692841979197E-5	0.0	0.0	0.0	4.278771367916788E-5
6	1.069692841979197E-5	0.0	0.0	0.0	7.48784989385438E-5
7	1.069692841979197E-5	0.0	0.0	0.0	7.48784989385438E-5
8	1.069692841979197E-5	0.0	0.0	0.0	9.627235577812773E-5
9	1.069692841979197E-5	0.0	0.0	2.139385683958394E-5	1.1766621261771168E-4
10	1.069692841979197E-5	0.0	0.0	1.3906006945729561E-4	1.1766621261771168E-4
11	1.069692841979197E-5	0.0	0.0	2.3533242523542336E-4	1.1766621261771168E-4
12	1.069692841979197E-5	0.0	0.0	3.8508942311251093E-4	1.2836314103750363E-4
13	1.069692841979197E-5	0.0	0.0	4.8136177889063865E-4	1.2836314103750363E-4
14	2.139385683958394E-5	0.0	0.0	8.022696314843977E-4	1.7115085471667153E-4
15	2.139385683958394E-5	0.0	0.0	0.001433388408252124	2.0324163997604744E-4
16	2.139385683958394E-5	0.0	0.0	0.0031448969554188394	2.2463549681563138E-4
17	2.139385683958394E-5	0.0	0.0	0.0051345256415001456	2.2463549681563138E-4
18	3.209078525937591E-5	0.0	0.0	0.006963700401284573	2.4602935365521534E-4
19	3.209078525937591E-5	0.0	0.0	0.01134944105339928	2.4602935365521534E-4
20	4.278771367916788E-5	0.0	0.0	0.01530730456872231	2.6742321049479925E-4
21	4.278771367916788E-5	0.0	0.0	0.024667116936040283	2.7812013891459123E-4
22	4.278771367916788E-5	1.069692841979197E-5	0.0	0.0363695566272927	2.7812013891459123E-4
23	4.278771367916788E-5	1.069692841979197E-5	0.0	0.05210473833280669	3.5299863785313504E-4
24	4.278771367916788E-5	1.069692841979197E-5	0.0	0.07576634399738652	3.5299863785313504E-4
25	4.278771367916788E-5	1.069692841979197E-5	0.0	0.09133037484818385	3.63695566272927E-4
26	4.278771367916788E-5	1.069692841979197E-5	0.0	0.12391321881487019	3.63695566272927E-4
27	4.278771367916788E-5	1.069692841979197E-5	0.0	0.1923842576299586	3.63695566272927E-4
28	4.278771367916788E-5	1.069692841979197E-5	0.0	0.31429715083032767	3.63695566272927E-4
29	4.278771367916788E-5	1.069692841979197E-5	0.0	0.4211059811019505	3.63695566272927E-4
30	4.278771367916788E-5	1.069692841979197E-5	0.0	0.5965676979717982	3.63695566272927E-4
31	4.278771367916788E-5	1.069692841979197E-5	0.0	0.9534493208413177	3.7439249469271895E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGT	5585	0.0	16.429724	1
GTATACG	155	7.228664E-9	15.5161295	1
TGGGCTG	8500	0.0	15.474706	36
AAAAGTA	5985	0.0	15.455304	2
TATAACG	360	0.0	15.416666	2
GGGCTGT	8995	0.0	15.116732	37
AAGACGG	2685	0.0	14.882682	5
ACGAACG	1260	0.0	14.829366	15
CGAACGA	1240	0.0	14.770162	16
CAAGACG	2770	0.0	14.559566	4
AAGTACT	6840	0.0	14.145468	4
AGTACTC	6910	0.0	14.055716	5
TATACCG	280	0.0	13.875001	5
AAAGTAC	6755	0.0	13.803108	3
AGGTTAT	2045	0.0	13.569682	1
CTTATAC	9380	0.0	13.490405	37
AACGAAC	1400	0.0	13.478572	14
TAACGAA	1415	0.0	13.335689	13
GGTTATC	2160	0.0	13.018518	2
CGAGCCG	2735	0.0	12.987203	15
>>END_MODULE