FastQCFastQC Report
Thu 9 Feb 2017
SRR2727178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579454
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT294680.643482825681839No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC288320.6295947071419432No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG267110.5832791420112529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT204570.44671264303561076No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA184340.40253707101326924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175470.3831679497162762No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG143660.3137055203524263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138320.3020447415783628No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG132550.28944498623635045No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT118680.25915753275390474No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC117580.2567554996731052No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG107440.2346131220010071No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT93800.2048279117990922No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA87770.19166040318343627No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT85290.18624491041945176No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC74080.16176600965966687No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG73710.16095805307794336No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC69280.1512844107616323No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68670.14995237423500704No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60180.13141304618410843No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT59240.12936039973324331No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT56610.12361735700369521No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT54880.11983961406752856No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC53740.11735023432924536No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC51770.11304841144817701No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT50970.11130147829850458No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC50780.11088658167545738No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT49110.10723985872551618No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC49080.10717434873240346No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA46810.10221742592020795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG652.6857524E-622.769235
AAGACGG11400.015.4166685
TCTAGCG8400.015.19642828
CTAGCGG8750.014.58857129
TCGTTAC5900.014.4237323
CAAGACG12200.014.2540984
CGAGCCG13500.014.25185215
TAAGACT4950.014.2020214
TATAACG1854.9094524E-914.02
CCGGTCC10200.013.9656869
ATAAGAC2800.013.8753
CACTCTA12200.013.799189
CGTTACA2551.8189894E-1213.78431324
CGGTCCA9800.013.78061310
CTAAGCG2051.4279067E-913.5365859
TAGCGGC9600.013.48958430
GACGGAC13200.013.1742427
AGTGTAC11400.013.14473727
CGCCGGT9500.013.0473687
AGGCCCG15600.012.92628310
Produced by FastQC (version 0.11.5)