FastQCFastQC Report
Thu 9 Feb 2017
SRR2727177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015433
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104090.34519088966659184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84390.27986030530275424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53500.1774206225109296No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48090.15947958386075897No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT45700.15155369063083146No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37460.12422759849083033No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG33550.11126096981760164No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT31900.10578911884296549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT200.001842410237.01
AAGACGG11400.019.3114055
CAAGACG11450.019.0655024
ACGAACG3450.018.23188415
CGCAAGA12450.016.9397582
GCGCAAG12900.016.779071
ACGGACC12350.016.7773288
CGAACGA3800.016.55263116
AGGTTAT5450.016.2935771
GACGGAC12800.016.18757
GCAAGAC15200.015.9440793
TATCTAG8700.015.5229891
CGGACCA13600.015.5073529
CGCTTCG7650.015.47712432
TAACGAA4100.015.34146313
AGACGGA14050.015.2740216
GGTTATC5700.015.2543872
GAGCGAA13850.015.22743816
ATACCGT7500.015.0466666
TACCGTC7400.015.0000017
Produced by FastQC (version 0.11.5)