##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3015433
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206606812354977	31.0	31.0	33.0	30.0	34.0
2	31.438564212834443	31.0	31.0	34.0	30.0	34.0
3	31.59812637190082	31.0	31.0	34.0	30.0	34.0
4	35.33762547534633	37.0	35.0	37.0	33.0	37.0
5	35.13031130189263	37.0	35.0	37.0	32.0	37.0
6	35.211913181291045	37.0	35.0	37.0	32.0	37.0
7	35.10551221002092	37.0	35.0	37.0	32.0	37.0
8	35.08630568147261	37.0	35.0	37.0	32.0	37.0
9	36.569213442978175	38.0	35.0	39.0	32.0	39.0
10	36.31030402598897	38.0	35.0	39.0	32.0	39.0
11	36.43071492551816	38.0	35.0	39.0	32.0	39.0
12	36.23890366657127	38.0	35.0	39.0	32.0	39.0
13	36.25479889621159	38.0	35.0	39.0	32.0	39.0
14	37.16915845916656	39.0	36.0	40.0	31.0	41.0
15	37.104162155153176	39.0	36.0	40.0	31.0	41.0
16	37.01607530328148	39.0	36.0	40.0	31.0	41.0
17	37.30326125634362	39.0	36.0	40.0	32.0	41.0
18	37.3291530602736	39.0	36.0	40.0	32.0	41.0
19	37.40042010550392	39.0	36.0	41.0	32.0	41.0
20	37.36922392240186	39.0	36.0	41.0	32.0	41.0
21	37.31639237217342	39.0	36.0	40.0	31.0	41.0
22	37.25101569161046	39.0	36.0	40.0	31.0	41.0
23	37.10652931104753	39.0	36.0	40.0	31.0	41.0
24	36.977486483699025	39.0	36.0	40.0	31.0	41.0
25	36.896770049276505	39.0	36.0	40.0	30.0	41.0
26	36.57617728531856	39.0	36.0	40.0	30.0	41.0
27	36.364135432622774	39.0	35.0	40.0	30.0	41.0
28	36.136735586564185	39.0	35.0	40.0	29.0	41.0
29	35.942861937240856	38.0	35.0	40.0	27.0	41.0
30	35.79322571584247	38.0	35.0	40.0	27.0	41.0
31	35.624105393819065	38.0	34.0	40.0	27.0	41.0
32	35.44087134418175	38.0	34.0	40.0	26.0	41.0
33	35.26735563350272	38.0	34.0	40.0	25.0	41.0
34	35.13577254079265	38.0	34.0	40.0	25.0	41.0
35	34.950920813030834	38.0	34.0	40.0	25.0	41.0
36	34.836564102070916	38.0	33.0	40.0	24.0	41.0
37	34.732483527241364	38.0	33.0	40.0	24.0	41.0
38	34.613648520792864	38.0	33.0	40.0	24.0	41.0
39	34.45832124275353	38.0	33.0	40.0	23.0	41.0
40	34.332578438983724	38.0	33.0	40.0	23.0	41.0
41	34.088937475977744	38.0	33.0	40.0	21.0	41.0
42	33.876445273365384	38.0	32.0	40.0	20.0	41.0
43	33.58128998389286	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	19.0
11	45.0
12	43.0
13	35.0
14	100.0
15	171.0
16	270.0
17	562.0
18	837.0
19	1584.0
20	2982.0
21	5245.0
22	8803.0
23	13593.0
24	20034.0
25	27469.0
26	36699.0
27	47863.0
28	61144.0
29	75612.0
30	91792.0
31	111779.0
32	135406.0
33	163235.0
34	198655.0
35	245465.0
36	309650.0
37	411039.0
38	627234.0
39	418060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.49606341775792	23.33183990491581	13.894057669329746	24.27803900799653
2	20.284317376642093	24.747026380622618	31.999384499672185	22.969271743063103
3	20.060502090412886	26.26713974410972	30.209127511703958	23.463230653773437
4	15.43784922430709	17.711917326632694	34.356691062278614	32.4935423867816
5	13.123256262035998	39.840745922724864	33.97299823939049	13.062999575848643
6	33.65155186668051	38.69470818950379	14.375248927765929	13.278491016049768
7	28.088204911201807	32.685853076490176	21.25048044509694	17.975461567211077
8	27.256185098458495	35.68419527145853	19.228515440402756	17.83110418968022
9	27.642829404599606	13.903376397353215	18.43022212730311	40.023572070744066
10	16.342263283581495	28.072817403006468	33.358426468105904	22.22649284530613
11	36.99475332398365	22.512355605314394	20.58987880015905	19.903012270542902
12	21.09090137303664	26.922435351738873	29.539969881605728	22.446693393618762
13	30.154508490157134	20.455403917115717	24.94102836972335	24.449059223003793
14	22.40829094859677	21.575840020322122	24.8441600261057	31.171709004975405
15	25.155856555260886	29.146792517028235	21.596400914893483	24.100950012817396
16	24.6561273289773	27.02019908915237	24.103503543272225	24.220170038598106
17	23.918389166663626	27.581014069952808	24.82389759613296	23.676699167250607
18	24.438811938451295	25.6020279674594	26.14327693568386	23.815883158405445
19	24.9590025711067	26.157702724616993	25.828463109609796	23.054831594666503
20	24.49173966060596	25.28492591279594	25.938198593701138	24.285135832896966
21	25.018695490830005	26.23281631526882	25.502539767920563	23.245948425980615
22	24.754919111119364	25.650246581502557	25.458997099255733	24.13583720812235
23	23.745644489531024	25.429482266725874	25.892964625644144	24.931908618098962
24	24.18627772528854	26.276856424931346	25.543827370729176	23.993038479050934
25	24.565593067396954	25.515075281062455	25.312086191270044	24.607245460270548
26	24.442227699968793	26.090680840860998	25.676942581712147	23.790148877458066
27	25.031861095902315	25.907987343774508	25.029141751781587	24.031009808541594
28	24.36877224597595	25.930438514137105	25.941780168884538	23.759009071002406
29	24.505369543942777	25.97643522505723	25.800672739205282	23.71752249179471
30	24.494492167459864	26.278680375256226	25.556959813068303	23.669867644215607
31	24.961124986030196	25.7425716306746	25.35407021147543	23.94223317181977
32	24.02938483461579	26.06046959093437	25.23063851858091	24.679507055868925
33	24.179180900388104	25.598645368675076	25.685233265007053	24.536940465929767
34	24.683353932917758	25.69412087749918	25.9000282878114	23.722496901771652
35	24.991535212355902	25.803458408792366	25.78017817010028	23.424828208751446
36	24.398817682236682	25.99006510839405	25.72817900447465	23.88293820489462
37	24.580648948260496	25.75776016247086	25.80083855287118	23.860752336397457
38	24.418251043879934	25.212299527132586	26.258849060814814	24.110600368172662
39	24.736812258803294	25.147366895566904	25.69617696695632	24.419643878673476
40	24.6097658279922	25.54568448378724	26.16271029732712	23.681839390893447
41	23.8008272775419	25.763994756308627	26.419953618601376	24.0152243475481
42	24.78453343184876	25.7167046987945	26.256262367626803	23.242499501729935
43	24.297472369639785	25.059850442705905	26.186023698752386	24.456653488901924
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1324.0
1	1040.0
2	756.0
3	706.5
4	657.0
5	657.0
6	935.5
7	1214.0
8	1559.5
9	1905.0
10	2683.5
11	3462.0
12	3462.0
13	7345.0
14	11228.0
15	15750.0
16	20272.0
17	17793.0
18	15314.0
19	15314.0
20	19342.5
21	23371.0
22	22098.0
23	20825.0
24	25492.0
25	30159.0
26	30159.0
27	36678.5
28	43198.0
29	50037.0
30	56876.0
31	65397.0
32	73918.0
33	73918.0
34	86655.5
35	99393.0
36	116501.5
37	133610.0
38	148829.0
39	164048.0
40	164048.0
41	176392.5
42	188737.0
43	197419.0
44	206101.0
45	220281.0
46	234461.0
47	234461.0
48	255708.5
49	276956.0
50	273609.5
51	270263.0
52	253128.0
53	235993.0
54	235993.0
55	225729.0
56	215465.0
57	202910.0
58	190355.0
59	174041.5
60	157728.0
61	157728.0
62	134014.5
63	110301.0
64	94426.0
65	78551.0
66	65522.5
67	52494.0
68	52494.0
69	42805.0
70	33116.0
71	29246.5
72	25377.0
73	19997.0
74	14617.0
75	14617.0
76	11318.0
77	8019.0
78	6935.5
79	5852.0
80	4591.5
81	3331.0
82	3331.0
83	2775.5
84	2220.0
85	1799.5
86	1379.0
87	1295.5
88	1212.0
89	1212.0
90	865.5
91	519.0
92	448.5
93	378.0
94	359.0
95	340.0
96	340.0
97	197.0
98	54.0
99	69.0
100	84.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3015433.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.142581386065515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08231153434508	45.81373125525074
2	8.88359180881647	9.797283686364944
3	2.9367146761501357	4.858140841117856
4	1.437282905221815	3.1702195830397844
5	0.8178937993635855	2.255038769828243
6	0.5305668350940952	1.7554094930954005
7	0.38762931857488037	1.4962416872998319
8	0.29866663639117996	1.3175399443602462
9	0.22405769746998327	1.1119607836121699
>10	1.2589247495014622	12.562756759957505
>50	0.07894585716984807	3.0020591943342416
>100	0.05177274155258514	5.656267463832645
>500	0.008221017153764694	3.1773336887300365
>1k	0.0032404009217398616	3.225313670772682
>5k	1.2001484895332822E-4	0.45627308566854596
>10k+	6.000742447666411E-5	0.3444300927350711
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10409	0.34519088966659184	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8439	0.27986030530275424	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5350	0.1774206225109296	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4809	0.15947958386075897	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4570	0.15155369063083146	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	3746	0.12422759849083033	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3355	0.11126096981760164	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	3190	0.10578911884296549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.316273317961301E-5
5	0.0	0.0	0.0	0.0	3.316273317961301E-5
6	0.0	0.0	0.0	0.0	3.316273317961301E-5
7	0.0	0.0	0.0	0.0	3.316273317961301E-5
8	0.0	0.0	0.0	0.0	6.632546635922602E-5
9	0.0	0.0	0.0	0.0	1.3265093271845203E-4
10	0.0	0.0	0.0	0.0	1.3265093271845203E-4
11	0.0	0.0	0.0	0.0	1.3265093271845203E-4
12	0.0	0.0	0.0	3.316273317961301E-5	1.6581366589806505E-4
13	0.0	0.0	0.0	3.316273317961301E-5	1.6581366589806505E-4
14	0.0	0.0	0.0	3.316273317961301E-5	2.3213913225729108E-4
15	0.0	0.0	0.0	6.632546635922602E-5	2.6530186543690407E-4
16	0.0	0.0	0.0	1.6581366589806505E-4	4.311155313349691E-4
17	0.0	0.0	0.0	2.3213913225729108E-4	4.311155313349691E-4
18	0.0	0.0	0.0	2.984645986165171E-4	4.974409976941952E-4
19	0.0	0.0	0.0	3.6479006497574314E-4	4.974409976941952E-4
20	0.0	0.0	0.0	5.306037308738081E-4	4.974409976941952E-4
21	0.0	0.0	0.0	6.632546635922602E-4	5.637664640534212E-4
22	0.0	0.0	0.0	9.948819953883904E-4	5.637664640534212E-4
23	0.0	0.0	0.0	0.0015918111926214245	7.295801299514863E-4
24	0.0	0.0	0.0	0.002586693188009815	7.295801299514863E-4
25	0.0	3.316273317961301E-5	0.0	0.00308413418570401	7.295801299514863E-4
26	0.0	3.316273317961301E-5	0.0	0.0037473888492962704	7.295801299514863E-4
27	0.0	3.316273317961301E-5	0.0	0.0068646857681798935	7.295801299514863E-4
28	0.0	3.316273317961301E-5	0.0	0.012502350408714105	7.295801299514863E-4
29	0.0	3.316273317961301E-5	0.0	0.020196104506384323	7.295801299514863E-4
30	0.0	3.316273317961301E-5	0.0	0.03684379656255005	7.295801299514863E-4
31	0.0	3.316273317961301E-5	0.0	0.09918973494022251	7.295801299514863E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAT	20	0.0018424102	37.0	1
AAGACGG	1140	0.0	19.311405	5
CAAGACG	1145	0.0	19.065502	4
ACGAACG	345	0.0	18.231884	15
CGCAAGA	1245	0.0	16.939758	2
GCGCAAG	1290	0.0	16.77907	1
ACGGACC	1235	0.0	16.777328	8
CGAACGA	380	0.0	16.552631	16
AGGTTAT	545	0.0	16.293577	1
GACGGAC	1280	0.0	16.1875	7
GCAAGAC	1520	0.0	15.944079	3
TATCTAG	870	0.0	15.522989	1
CGGACCA	1360	0.0	15.507352	9
CGCTTCG	765	0.0	15.477124	32
TAACGAA	410	0.0	15.341463	13
AGACGGA	1405	0.0	15.274021	6
GGTTATC	570	0.0	15.254387	2
GAGCGAA	1385	0.0	15.227438	16
ATACCGT	750	0.0	15.046666	6
TACCGTC	740	0.0	15.000001	7
>>END_MODULE