##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5677213
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15488973198645	31.0	31.0	33.0	30.0	34.0
2	31.291146025347295	31.0	31.0	34.0	30.0	34.0
3	31.34667450384546	31.0	31.0	34.0	28.0	34.0
4	35.18565377060892	35.0	35.0	37.0	33.0	37.0
5	35.042575820213195	35.0	35.0	37.0	32.0	37.0
6	35.06426410282651	36.0	35.0	37.0	32.0	37.0
7	34.882434039378126	36.0	35.0	37.0	32.0	37.0
8	34.804289005890745	36.0	35.0	37.0	32.0	37.0
9	36.43287683587	38.0	35.0	39.0	32.0	39.0
10	36.21367191965494	38.0	35.0	39.0	32.0	39.0
11	36.33147021258494	38.0	35.0	39.0	32.0	39.0
12	36.19141222990929	38.0	35.0	39.0	31.0	39.0
13	36.295385253292416	38.0	35.0	39.0	32.0	39.0
14	37.26254290617597	39.0	36.0	41.0	32.0	41.0
15	37.15716796251964	39.0	36.0	40.0	31.0	41.0
16	37.122329565580856	39.0	36.0	40.0	31.0	41.0
17	37.02981762354169	39.0	36.0	40.0	31.0	41.0
18	37.04417079295774	39.0	36.0	40.0	31.0	41.0
19	37.061893573484035	39.0	36.0	40.0	31.0	41.0
20	36.94074962485995	39.0	36.0	40.0	31.0	41.0
21	36.81434552482001	39.0	36.0	40.0	30.0	41.0
22	36.758067734996025	39.0	35.0	40.0	30.0	41.0
23	36.543938724863764	38.0	35.0	40.0	30.0	41.0
24	36.424893693437255	38.0	35.0	40.0	30.0	41.0
25	36.303009416768404	38.0	35.0	40.0	30.0	41.0
26	35.99788628681009	38.0	34.0	40.0	29.0	41.0
27	36.14075709331321	38.0	35.0	40.0	29.0	41.0
28	36.009916309287675	38.0	34.0	40.0	29.0	41.0
29	35.91283311018981	38.0	34.0	40.0	28.0	41.0
30	36.04710585986469	38.0	35.0	40.0	29.0	41.0
31	35.993101016290915	38.0	35.0	40.0	29.0	41.0
32	35.823968908688116	38.0	34.0	40.0	28.0	41.0
33	35.73681910472621	38.0	34.0	40.0	27.0	41.0
34	35.76265343576153	38.0	34.0	40.0	27.0	41.0
35	35.469765358460215	38.0	34.0	40.0	26.0	41.0
36	35.32955642143425	38.0	34.0	40.0	26.0	41.0
37	35.138287924021874	38.0	33.0	40.0	25.0	41.0
38	34.99613243329077	38.0	33.0	40.0	25.0	41.0
39	34.92748519387946	38.0	33.0	40.0	25.0	41.0
40	34.79579593015094	38.0	33.0	40.0	24.0	41.0
41	34.62770095115332	38.0	33.0	40.0	24.0	41.0
42	34.46631031810855	38.0	33.0	40.0	23.0	41.0
43	34.050105395023934	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	15.0
14	29.0
15	69.0
16	178.0
17	452.0
18	824.0
19	1747.0
20	3308.0
21	6424.0
22	11197.0
23	18533.0
24	29135.0
25	43844.0
26	63786.0
27	88073.0
28	118851.0
29	153598.0
30	193786.0
31	236459.0
32	280865.0
33	334245.0
34	400441.0
35	475858.0
36	577501.0
37	725362.0
38	949832.0
39	962793.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.38044265733908	19.368711372992347	12.329165736779649	25.92168023288892
2	17.70763224842894	23.51298075305612	33.643884772334594	25.135502226180346
3	20.727370982910102	25.19368218173248	29.515204027046373	24.56374280831105
4	14.96736162620638	16.38525804827122	33.701272085440515	34.946108240081884
5	14.1631289860007	37.86976109580528	33.26554772561819	14.701562192575828
6	34.21721186081974	34.04036452393102	16.23130927093981	15.511114344309435
7	27.434218163031755	31.469719385198335	22.151819915863648	18.944242535906263
8	27.639706313643686	32.6564460413939	21.417551182243823	18.28629646271859
9	28.145623565647437	13.45846985131613	19.510294928162818	38.885611654873614
10	17.364259540728874	26.56514737072574	32.73231073063491	23.338282357910476
11	35.62069980464006	22.005709491611462	20.868338038400182	21.505252665348294
12	21.72215486718571	26.297040466862875	29.270488882485118	22.71031578346629
13	30.411330348183167	19.01103587270726	25.311662606282344	25.26597117282723
14	22.89863001441024	21.396819178706174	25.384832311206218	30.319718495677368
15	26.666816975160174	26.501189932454533	22.73909751140216	24.092895580983136
16	23.9042642930607	25.64154277812018	26.47022403422243	23.983968894596696
17	24.35053960455597	26.30787324696114	25.870422688033724	23.471164460449167
18	24.650634034692725	23.48814462307474	28.026780041545035	23.8344413006875
19	24.540421506115763	26.36337583247273	27.041384566687913	22.05481809472359
20	24.087382312412796	24.247249486675944	27.228377726888176	24.436990474023084
21	24.307701683907226	25.964606224920573	26.974961129695153	22.75273096147705
22	24.547713816620938	25.361035423543203	25.1899303408204	24.90132041901546
23	22.437417796372973	25.191233797287506	26.806656716948968	25.56469168939055
24	23.040266412410457	27.228694783866665	26.204970643165932	23.52606816055695
25	24.284891195732836	25.015179102845003	25.087538551046084	25.612391150376073
26	23.7232599868985	25.81322913197021	26.646948071175064	23.816562809956224
27	25.11263889517621	25.231711404874186	25.235199031637528	24.420450668312075
28	23.180211839858746	26.17092224653188	26.81990265293904	23.82896326067033
29	23.673992855297133	25.439595097101343	27.132309462406994	23.75410258519453
30	23.481803483505022	26.709725352915243	27.20812130881825	22.600349854761483
31	24.90507225992754	25.57090952902419	25.126325188080838	24.39769302296743
32	22.200417704954877	26.32166522552527	26.25742595882874	25.220491110691107
33	22.846914498363898	25.553242409611897	26.963001035895605	24.636842056128597
34	23.431144824053636	25.620828388859113	27.06787643866806	23.8801503484192
35	23.596578109716862	26.18851890883784	27.49650224502762	22.718400736417678
36	23.542995480352772	26.018100782901747	26.485900740380885	23.95300299636459
37	23.211706166388332	25.87162045884134	27.237378622221854	23.679294752548476
38	23.39966811180063	25.126765544995404	27.574057904820553	23.89950843838341
39	23.588951127956623	23.981414824492227	27.257018540611387	25.17261550693976
40	23.540071510440068	24.88081740107338	27.871193136491446	23.707917951995107
41	22.058305721486935	25.0449472302695	28.418873838272408	24.47787320997116
42	23.523126576367666	25.457033230918057	27.95412115064205	23.065719042072228
43	22.385015323539914	23.84587296618957	28.41038375695962	25.358727953310893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1414.0
1	1589.0
2	1764.0
3	4646.0
4	7528.0
5	7528.0
6	11287.5
7	15047.0
8	14637.0
9	14227.0
10	17832.5
11	21438.0
12	21438.0
13	31824.0
14	42210.0
15	56869.0
16	71528.0
17	64696.0
18	57864.0
19	57864.0
20	58744.5
21	59625.0
22	51672.0
23	43719.0
24	48573.5
25	53428.0
26	53428.0
27	59762.5
28	66097.0
29	72720.5
30	79344.0
31	89389.0
32	99434.0
33	99434.0
34	117040.0
35	134646.0
36	162654.0
37	190662.0
38	218397.5
39	246133.0
40	246133.0
41	268238.0
42	290343.0
43	312234.5
44	334126.0
45	378183.0
46	422240.0
47	422240.0
48	534956.5
49	647673.0
50	648904.0
51	650135.0
52	544349.0
53	438563.0
54	438563.0
55	411078.0
56	383593.0
57	368708.5
58	353824.0
59	329387.0
60	304950.0
61	304950.0
62	253520.5
63	202091.0
64	176690.5
65	151290.0
66	128093.5
67	104897.0
68	104897.0
69	87694.5
70	70492.0
71	60557.5
72	50623.0
73	37684.5
74	24746.0
75	24746.0
76	19286.0
77	13826.0
78	12138.0
79	10450.0
80	8545.5
81	6641.0
82	6641.0
83	5643.5
84	4646.0
85	4135.5
86	3625.0
87	2449.5
88	1274.0
89	1274.0
90	918.5
91	563.0
92	393.0
93	223.0
94	232.0
95	241.0
96	241.0
97	128.0
98	15.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5677213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.86704501993893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17457289496053	36.04755688677487
2	8.971829416139771	7.871352898180314
3	3.013456269587098	3.9657426553078334
4	1.5469610022793674	2.714424317243154
5	0.887651837877869	1.9469331567110009
6	0.6109269815873466	1.6079736843112522
7	0.43172291915567096	1.3256886111518893
8	0.345708568909854	1.213217068491778
9	0.2458923931863497	0.9707915413769515
>10	1.557386080221665	12.593953232271845
>50	0.10672957546858212	3.217931769363428
>100	0.08086961674786299	7.3481346515136545
>500	0.01351139681735364	4.121827272137566
>1k	0.011807252998916669	10.092197591026538
>5k	5.274717834566332E-4	1.5580840455002982
>10k+	4.4632227830945884E-4	3.404190618637575
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29541	0.5203433445248575	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	24726	0.4355306027799204	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	24214	0.4265120931696591	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20260	0.35686524356229016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18231	0.3211258763763135	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	15860	0.2793624265991077	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13653	0.24048771818143866	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	13074	0.23028905204014716	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	11342	0.19978112499918535	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	10336	0.18206116275714862	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	10021	0.17651266563364804	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	9156	0.16127631638974968	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	8605	0.15157084999276935	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	7577	0.1334633736659167	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	7272	0.12809101930824157	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	7240	0.12752736245760024	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7136	0.12569547769301592	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	6595	0.11616615406186098	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	6407	0.1128546700643432	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	6305	0.11105801385292396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7614276582541468E-5
2	0.0	0.0	0.0	1.7614276582541468E-5	1.7614276582541468E-5
3	0.0	0.0	0.0	1.7614276582541468E-5	1.7614276582541468E-5
4	0.0	0.0	0.0	3.5228553165082935E-5	1.7614276582541468E-5
5	0.0	0.0	0.0	3.5228553165082935E-5	1.7614276582541468E-5
6	0.0	0.0	0.0	3.5228553165082935E-5	1.7614276582541468E-5
7	0.0	0.0	0.0	3.5228553165082935E-5	1.7614276582541468E-5
8	0.0	0.0	0.0	3.5228553165082935E-5	1.7614276582541468E-5
9	0.0	0.0	0.0	5.284282974762441E-5	1.7614276582541468E-5
10	0.0	0.0	0.0	7.045710633016587E-5	1.7614276582541468E-5
11	0.0	0.0	0.0	8.807138291270734E-5	3.5228553165082935E-5
12	0.0	0.0	0.0	1.2329993607779029E-4	3.5228553165082935E-5
13	0.0	0.0	0.0	1.7614276582541468E-4	3.5228553165082935E-5
14	0.0	0.0	0.0	4.051283613984538E-4	1.0568565949524882E-4
15	0.0	3.5228553165082935E-5	0.0	8.454852759619906E-4	1.2329993607779029E-4
16	0.0	3.5228553165082935E-5	0.0	0.0021665560196526008	1.5852848924287322E-4
17	0.0	3.5228553165082935E-5	0.0	0.0033995553804305034	1.5852848924287322E-4
18	0.0	5.284282974762441E-5	0.0	0.004350726315887743	1.7614276582541468E-4
19	0.0	5.284282974762441E-5	0.0	0.006429210952627636	1.7614276582541468E-4
20	0.0	5.284282974762441E-5	0.0	0.007908810185561119	2.1137131899049764E-4
21	0.0	5.284282974762441E-5	0.0	0.012928879011585438	2.289855955730391E-4
22	0.0	5.284282974762441E-5	0.0	0.019639918389533738	2.289855955730391E-4
23	0.0	5.284282974762441E-5	0.0	0.029486298999174418	3.5228553165082935E-4
24	0.0	5.284282974762441E-5	0.0	0.04451127692408229	3.5228553165082935E-4
25	0.0	5.284282974762441E-5	0.0	0.053635472193838775	3.5228553165082935E-4
26	0.0	5.284282974762441E-5	0.0	0.07052756343649604	3.5228553165082935E-4
27	0.0	5.284282974762441E-5	0.0	0.11695879650807535	3.5228553165082935E-4
28	0.0	5.284282974762441E-5	0.0	0.18951200175156366	3.5228553165082935E-4
29	0.0	5.284282974762441E-5	0.0	0.2585071231253786	3.5228553165082935E-4
30	0.0	5.284282974762441E-5	0.0	0.3657604532364736	3.5228553165082935E-4
31	0.0	5.284282974762441E-5	0.0	0.6480996925780308	3.5228553165082935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1995	0.0	16.598997	5
CAAGACG	2060	0.0	16.254854	4
ACCGTCG	1480	0.0	14.75	8
TACCGTC	1485	0.0	13.952862	7
ATACCGT	1530	0.0	13.905229	6
ACGAACG	1030	0.0	13.830096	15
TACTTAC	1320	0.0	13.734849	31
CGCAAGA	2410	0.0	13.6639	2
CGAACGA	1030	0.0	13.650485	16
TGTTACG	485	0.0	13.350515	24
CGGACCA	2445	0.0	13.316973	9
AACGAAC	1080	0.0	13.1898155	14
CGTCGTA	1575	0.0	13.155556	10
CCGTCGT	1590	0.0	13.1477995	9
TTAGAGT	1170	0.0	13.123932	4
GCAAGAC	2730	0.0	13.078755	3
ACGGACC	2435	0.0	13.067762	8
GTCCTAT	1530	0.0	13.058824	1
TCTTATA	6265	0.0	13.051876	37
GCGCAAG	2475	0.0	13.006061	1
>>END_MODULE