FastQCFastQC Report
Thu 9 Feb 2017
SRR2727175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467716
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT518561.1606825500994244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT356630.7982378468103166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT319480.7150857395590946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254010.5685455387047879No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC184540.41305221728507363No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG149950.33563010719571257No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA119360.2671611176717589No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109410.24489023026530782No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG100150.2241637561563895No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC93980.2103535676842485No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT92060.20605606981285293No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG90630.20285532921071975No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA76770.17183276645158285No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG76510.17125081361483138No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.16265581787204023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT65530.14667449766278787No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA58360.130626029049295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.12968595138992722No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA57440.12856681131925127No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT56130.12563466433408033No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA53070.11878552710154361No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT50660.11339127196088562No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA48850.10933998490503871No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT45020.10076737196366106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10000.017.9455
CAAGACG11350.016.2995594
TATACTG4250.015.2352945
GCGTTAA1101.4536561E-515.1363641
TATAACG2700.015.0740752
GACGGAC11800.014.8940687
ACCGTCG7350.014.850348
AGTACTC17850.014.7170865
ACGGACC11900.014.6134468
TACCGTC8000.014.5687497
CGCAAGA12300.014.2886182
GCGCAAG12650.014.1857711
AAAGTAC18850.014.0344823
CCGTCGT7650.014.0261449
ATACCGT8250.013.903036
GTATTAG8550.013.6315791
AAAAAGT18200.013.6208781
CGTTAAT2051.4279067E-913.5365861
TATACAG5250.013.3904775
TATCACG2355.638867E-1113.3829792
Produced by FastQC (version 0.11.5)