FastQCFastQC Report
Thu 9 Feb 2017
SRR2727174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4198051
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT657651.5665602919068873No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC447191.065232413803453No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG414790.9880537420817422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT414430.9871962012848343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT372780.8879834951981289No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326700.7782182731939179No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA299020.7122829141427772No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG229750.5472777724710824No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG223670.5327948612344157No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT204230.4864876582013892No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC199880.4761257069054187No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG189170.45061386819740873No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT145430.3464226613730991No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA139070.33127277396105953No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138390.3296529746780113No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT132890.31655165694747395No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT120750.2876334756295243No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG112260.267409805169113No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC110820.26397964198148144No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC110210.26252658674227636No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC98040.233536943691251No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93480.22267476026375096No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT93110.22179339888915117No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT92340.21995921440687596No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT88890.21174111510317528No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC88080.20981164831013246No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT86520.20609563819019827No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT86460.20595271472404697No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC83310.19844923275110282No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA82090.19554312227269272No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC81160.19332780854734732No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79620.18965943958279685No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA78880.1878967168335973No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG72140.1718416474692661No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA71570.17048387454082858No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT68330.1627660073686575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC68240.16255162216943053No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT66860.15926438244795027No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT62680.14930738097274188No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62250.14828309613199075No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG61000.145305523920505No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA58730.1398982527844469No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG57930.13799260656909598No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG54800.13053676575153567No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT53430.12727334660774728No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA52660.12543916212547204No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT52010.12389082457549944No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50320.1198651469455707No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49350.11755455090945775No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT46850.11159940648648624No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT46400.11052748049035135No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA45570.10855037254192482No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT44980.10714495845810354No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG44710.1065018028604226No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG44290.1055013385973634No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA43230.10297635736202349No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT42250.10064194074821864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT902.1549859E-618.54
AAAAGTA26000.017.5038452
AAAAAGT27150.016.558011
TATACCG1151.2440396E-616.0869565
TGGGCTG40300.015.60794136
CGTTAAG1101.4536145E-515.13636424
GATTTCG1601.0988515E-815.03125119
GGGCTGT43550.014.86796937
TTATCGT750.00410681714.84
AGTACTC31800.014.7767295
AAGTACT32850.014.6423144
ACGGATG9000.014.38888928
TCGTTAC6700.014.35820923
GGCGTCA6350.014.27559112
AAAGTAC32500.014.1738463
TACGGAT8900.014.13483227
AAGACGG6550.014.1221375
GGGCGTC6600.014.01515111
ATACCGT4900.013.9693896
GAACAGT22150.013.9480811
Produced by FastQC (version 0.11.5)