##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727174.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4198051
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04360880799209	31.0	31.0	33.0	30.0	34.0
2	31.098329200860114	31.0	31.0	34.0	28.0	34.0
3	31.147914591795097	31.0	31.0	34.0	28.0	34.0
4	34.962436616420334	35.0	35.0	37.0	32.0	37.0
5	34.84726388507429	35.0	35.0	37.0	32.0	37.0
6	34.93909816722093	35.0	35.0	37.0	32.0	37.0
7	34.74676296214601	35.0	35.0	37.0	32.0	37.0
8	34.727640993403845	35.0	35.0	37.0	31.0	37.0
9	36.27801472635754	38.0	35.0	39.0	32.0	39.0
10	36.07330782784678	38.0	35.0	39.0	31.0	39.0
11	36.20834477713586	38.0	35.0	39.0	32.0	39.0
12	36.14140943023322	38.0	35.0	39.0	31.0	39.0
13	36.23314962109798	38.0	35.0	39.0	32.0	39.0
14	37.10442417207414	39.0	36.0	40.0	31.0	41.0
15	37.011840256347526	39.0	36.0	40.0	31.0	41.0
16	36.94369434768658	39.0	36.0	40.0	31.0	41.0
17	37.01317921101959	39.0	36.0	40.0	31.0	41.0
18	37.047354832039915	39.0	36.0	40.0	31.0	41.0
19	37.10237750803885	39.0	36.0	40.0	31.0	41.0
20	37.065087822896864	39.0	36.0	40.0	31.0	41.0
21	36.94183014927641	39.0	36.0	40.0	31.0	41.0
22	36.934450296101694	39.0	35.0	40.0	31.0	41.0
23	36.6899504079393	39.0	35.0	40.0	30.0	41.0
24	36.58314298706709	38.0	35.0	40.0	30.0	41.0
25	36.48690404189944	38.0	35.0	40.0	30.0	41.0
26	36.10622072004366	38.0	35.0	40.0	29.0	41.0
27	36.14045065198112	38.0	35.0	40.0	30.0	41.0
28	35.98607639592754	38.0	35.0	40.0	29.0	41.0
29	35.84240996595801	38.0	34.0	40.0	29.0	41.0
30	35.80131684917596	38.0	34.0	40.0	29.0	41.0
31	35.595862699143005	38.0	34.0	40.0	28.0	41.0
32	35.25580322868874	37.0	34.0	40.0	27.0	41.0
33	35.11546167495345	37.0	34.0	40.0	26.0	41.0
34	35.02827955163003	37.0	34.0	40.0	25.0	41.0
35	34.680070108724266	37.0	33.0	40.0	24.0	41.0
36	34.43405689926111	37.0	33.0	40.0	24.0	41.0
37	34.13308342371258	37.0	33.0	40.0	22.0	41.0
38	33.894149213527896	37.0	33.0	40.0	21.0	41.0
39	33.709919912835744	37.0	33.0	40.0	20.0	41.0
40	33.45860233713216	37.0	33.0	40.0	18.0	41.0
41	33.18528336125502	37.0	32.0	40.0	15.0	41.0
42	32.90900229654189	37.0	31.0	40.0	15.0	41.0
43	32.40791262421538	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	6.0
14	15.0
15	47.0
16	131.0
17	332.0
18	734.0
19	1683.0
20	3337.0
21	5915.0
22	10298.0
23	16448.0
24	25941.0
25	38522.0
26	55000.0
27	77014.0
28	102836.0
29	131321.0
30	161216.0
31	189597.0
32	221221.0
33	260383.0
34	306128.0
35	360014.0
36	439671.0
37	568667.0
38	657566.0
39	564003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.322198324889335	19.18411662935967	12.752822678905043	22.74086236684595
2	15.072303790497067	28.152492668621704	32.3421273348037	24.433076206077533
3	23.043788653353666	29.4063602371672	29.33096810877238	18.218883000706757
4	16.010786910401993	18.102305093482666	30.707440190698016	35.179467805417325
5	12.881048848620466	40.84602592965164	31.864429469770617	14.408495751957275
6	31.453143375342513	31.05455364882418	18.66397049487965	18.828332480953662
7	23.473488054337597	33.17934917894042	24.133651544490526	19.213511222231457
8	28.905508770617605	30.819706573359877	24.418069242131647	15.856715413890873
9	29.001815366225898	13.620915991730447	22.200516382483205	35.17675225956045
10	18.756870747878004	29.067441057767045	32.238793668776296	19.936894525578655
11	29.87631641445042	24.536767180770312	21.615125685705106	23.97179071907416
12	20.760181331765622	30.585097703672492	30.320284341471794	18.334436623090095
13	30.038796574886774	19.752880562908835	27.92593515419417	22.28238770801022
14	22.13441427938822	23.521486518386748	29.70850044461108	24.63559875761395
15	27.58713507768248	25.003436118332058	27.031663026485386	20.37776577750008
16	19.069754035860928	26.399536356275803	33.291639382179966	21.2390702256833
17	22.4145442730448	28.728093107968434	29.692564478135207	19.164798140851552
18	21.9504003167184	22.991121356076903	34.11359223601619	20.944886091188504
19	21.292071011047746	29.494806042137174	32.55534532572377	16.65777762109131
20	20.691601888590682	25.420058022163143	34.05971008927715	19.82862999996903
21	19.666721533397283	28.438673089012017	33.29240164066611	18.60220373692459
22	21.139571672664292	28.445509594809593	28.42002157667927	21.994897155846843
23	17.93539430559562	28.407206105881038	31.78100980669363	21.87638978182971
24	18.20704417359389	32.467637958662245	30.601915031522964	18.723402836220902
25	20.401967484435037	28.220000185800505	28.23607907574253	23.141953254021928
26	19.71936501009635	28.360922723425702	31.536967988240256	20.382744278237688
27	21.03256963767234	28.169095611272944	29.317890611619536	21.480444139435182
28	18.78905234833974	29.785274166512032	32.096823025732654	19.328850459415573
29	20.00425911929131	27.299763628407565	32.69602965757205	19.99994759472908
30	18.886835819764933	30.897409297790805	32.92535035901184	17.29040452343242
31	22.05309082714812	28.307874296905872	28.882402810256476	20.75663206568953
32	15.952974368343787	30.683667254161513	31.9848424900031	21.378515887491602
33	18.417022565947867	29.608168171372856	32.46123022326313	19.513579039416147
34	17.38373354682923	29.50133288042475	32.06507019566937	21.049863377076647
35	17.07554291265161	31.615075662491954	33.102003763174864	18.207377661681576
36	19.73363353613379	29.629082638586336	30.347916211594377	20.289367613685492
37	17.831155457615928	30.655654254795856	31.66293120307495	19.850259084513265
38	19.461292871382458	29.67860561960777	31.42460632326763	19.43549518574215
39	19.25774603500529	27.175277289389765	31.194118413520943	22.372858262084
40	18.712326267594175	29.253980001672204	31.59575717398383	20.437936556749786
41	17.211177282029208	29.27720506492179	31.544066520392438	21.96755113265656
42	18.831953208762826	30.337196951633032	30.820421190690634	20.010428648913507
43	17.943231275656252	27.489780376655737	31.707046912960323	22.859941434727688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3027.0
1	3325.0
2	3623.0
3	10660.0
4	17697.0
5	17697.0
6	27482.5
7	37268.0
8	37537.0
9	37806.0
10	45876.5
11	53947.0
12	53947.0
13	80090.5
14	106234.0
15	140520.5
16	174807.0
17	153136.5
18	131466.0
19	131466.0
20	127840.5
21	124215.0
22	100107.0
23	75999.0
24	76363.0
25	76727.0
26	76727.0
27	77036.0
28	77345.0
29	76671.0
30	75997.0
31	77450.5
32	78904.0
33	78904.0
34	85008.5
35	91113.0
36	101736.0
37	112359.0
38	124841.5
39	137324.0
40	137324.0
41	149807.0
42	162290.0
43	184411.0
44	206532.0
45	256577.5
46	306623.0
47	306623.0
48	469888.0
49	633153.0
50	620111.0
51	607069.0
52	422851.0
53	238633.0
54	238633.0
55	198900.0
56	159167.0
57	148132.5
58	137098.0
59	124232.5
60	111367.0
61	111367.0
62	93258.5
63	75150.0
64	64200.5
65	53251.0
66	44174.0
67	35097.0
68	35097.0
69	28758.5
70	22420.0
71	18724.5
72	15029.0
73	11322.5
74	7616.0
75	7616.0
76	5883.5
77	4151.0
78	3602.5
79	3054.0
80	2420.0
81	1786.0
82	1786.0
83	1500.0
84	1214.0
85	1059.5
86	905.0
87	614.0
88	323.0
89	323.0
90	231.5
91	140.0
92	98.5
93	57.0
94	57.5
95	58.0
96	58.0
97	31.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4198051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.159881696574914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4426635040213	28.98674295551923
2	8.681675044399597	6.104933349783932
3	2.923140784605392	3.083318525074762
4	1.4505369801913637	2.040028344801415
5	0.9039815971482146	1.5891943005806035
6	0.6309332125240394	1.3310122266471112
7	0.4393888007558246	1.0814200777362302
8	0.32756773985218035	0.9213794384653671
9	0.2563806148497683	0.8112880878671692
>10	1.6158660481843248	10.673298779552178
>50	0.1584176230681024	3.882734552968877
>100	0.13695427058759083	10.016314266591428
>500	0.01756608182173602	4.297570532614133
>1k	0.011595002625339268	8.009401662769518
>5k	0.0019440722964640688	4.992065000349677
>10k+	0.0013886230689029065	12.179297898678472
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	65765	1.5665602919068873	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	44719	1.065232413803453	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	41479	0.9880537420817422	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	41443	0.9871962012848343	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37278	0.8879834951981289	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32670	0.7782182731939179	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	29902	0.7122829141427772	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	22975	0.5472777724710824	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	22367	0.5327948612344157	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20423	0.4864876582013892	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	19988	0.4761257069054187	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	18917	0.45061386819740873	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	14543	0.3464226613730991	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	13907	0.33127277396105953	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13839	0.3296529746780113	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	13289	0.31655165694747395	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	12075	0.2876334756295243	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	11226	0.267409805169113	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	11082	0.26397964198148144	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	11021	0.26252658674227636	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	9804	0.233536943691251	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9348	0.22267476026375096	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	9311	0.22179339888915117	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9234	0.21995921440687596	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	8889	0.21174111510317528	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	8808	0.20981164831013246	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	8652	0.20609563819019827	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8646	0.20595271472404697	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	8331	0.19844923275110282	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	8209	0.19554312227269272	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	8116	0.19332780854734732	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7962	0.18965943958279685	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	7888	0.1878967168335973	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	7214	0.1718416474692661	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7157	0.17048387454082858	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	6833	0.1627660073686575	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	6824	0.16255162216943053	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	6686	0.15926438244795027	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	6268	0.14930738097274188	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6225	0.14828309613199075	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	6100	0.145305523920505	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	5873	0.1398982527844469	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	5793	0.13799260656909598	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	5480	0.13053676575153567	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	5343	0.12727334660774728	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	5266	0.12543916212547204	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	5201	0.12389082457549944	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	5032	0.1198651469455707	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4935	0.11755455090945775	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	4685	0.11159940648648624	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	4640	0.11052748049035135	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4557	0.10855037254192482	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	4498	0.10714495845810354	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4471	0.1065018028604226	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	4429	0.1055013385973634	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	4323	0.10297635736202349	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	4225	0.10064194074821864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3820577691886067E-5
2	0.0	0.0	0.0	0.0	2.3820577691886067E-5
3	0.0	0.0	0.0	0.0	2.3820577691886067E-5
4	0.0	0.0	0.0	2.3820577691886067E-5	2.3820577691886067E-5
5	0.0	0.0	0.0	2.3820577691886067E-5	2.3820577691886067E-5
6	0.0	0.0	0.0	2.3820577691886067E-5	2.3820577691886067E-5
7	0.0	0.0	0.0	2.3820577691886067E-5	2.3820577691886067E-5
8	2.3820577691886067E-5	0.0	0.0	4.764115538377213E-5	2.3820577691886067E-5
9	2.3820577691886067E-5	0.0	0.0	4.764115538377213E-5	2.3820577691886067E-5
10	2.3820577691886067E-5	0.0	0.0	2.858469323026328E-4	2.3820577691886067E-5
11	2.3820577691886067E-5	0.0	0.0	6.193350199890378E-4	2.3820577691886067E-5
12	2.3820577691886067E-5	0.0	0.0	0.00145305523920505	2.3820577691886067E-5
13	2.3820577691886067E-5	0.0	0.0	0.002048569681502202	2.3820577691886067E-5
14	2.3820577691886067E-5	0.0	0.0	0.0035969072314747962	4.764115538377213E-5
15	2.3820577691886067E-5	0.0	0.0	0.006836505797571301	4.764115538377213E-5
16	2.3820577691886067E-5	0.0	0.0	0.015697760698952918	7.14617330756582E-5
17	2.3820577691886067E-5	0.0	0.0	0.026917252791831256	7.14617330756582E-5
18	2.3820577691886067E-5	0.0	0.0	0.03237216508327317	9.528231076754427E-5
19	2.3820577691886067E-5	0.0	0.0	0.04544966223611862	1.1910288845943035E-4
20	2.3820577691886067E-5	0.0	0.0	0.057884003791283146	1.1910288845943035E-4
21	2.3820577691886067E-5	0.0	0.0	0.0880646757269028	1.1910288845943035E-4
22	2.3820577691886067E-5	0.0	0.0	0.12948866033309267	1.1910288845943035E-4
23	2.3820577691886067E-5	0.0	0.0	0.18627691755054906	1.6674404384320248E-4
24	2.3820577691886067E-5	0.0	0.0	0.27248358821748475	1.6674404384320248E-4
25	2.3820577691886067E-5	0.0	0.0	0.32476975625117466	1.6674404384320248E-4
26	2.3820577691886067E-5	0.0	0.0	0.4053547705828252	1.6674404384320248E-4
27	2.3820577691886067E-5	0.0	0.0	0.5416084749804135	1.6674404384320248E-4
28	4.764115538377213E-5	0.0	0.0	0.7696666858025307	1.6674404384320248E-4
29	4.764115538377213E-5	0.0	0.0	0.9510603849262431	1.6674404384320248E-4
30	4.764115538377213E-5	0.0	0.0	1.230189914319764	1.6674404384320248E-4
31	4.764115538377213E-5	0.0	0.0	1.6984548305868605	1.6674404384320248E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGT	90	2.1549859E-6	18.5	4
AAAAGTA	2600	0.0	17.503845	2
AAAAAGT	2715	0.0	16.55801	1
TATACCG	115	1.2440396E-6	16.086956	5
TGGGCTG	4030	0.0	15.607941	36
CGTTAAG	110	1.4536145E-5	15.136364	24
GATTTCG	160	1.0988515E-8	15.031251	19
GGGCTGT	4355	0.0	14.867969	37
TTATCGT	75	0.004106817	14.8	4
AGTACTC	3180	0.0	14.776729	5
AAGTACT	3285	0.0	14.642314	4
ACGGATG	900	0.0	14.388889	28
TCGTTAC	670	0.0	14.358209	23
GGCGTCA	635	0.0	14.275591	12
AAAGTAC	3250	0.0	14.173846	3
TACGGAT	890	0.0	14.134832	27
AAGACGG	655	0.0	14.122137	5
GGGCGTC	660	0.0	14.015151	11
ATACCGT	490	0.0	13.969389	6
GAACAGT	2215	0.0	13.948081	1
>>END_MODULE