FastQCFastQC Report
Thu 9 Feb 2017
SRR2727173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9589260
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC807350.8419314941924612No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG727640.7588072489430884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT674860.7037665054446329No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA522820.5452141249689757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT442910.46188131305231067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT398680.41575679458060366No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG376790.3929291728454542No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC369990.38583790615751373No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG360330.3757641361272924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338110.3525923793911105No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT313960.3274079543155572No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG299460.3122868709368606No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA243250.253669209094341No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT234010.24403342906543363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT211030.2200691189935407No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG208220.21713875731808296No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC196650.2050731756152195No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC190000.19813833392774835No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT183880.1917561939086019No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159790.16663433883323636No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT153440.1600123471467037No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC151020.15748869047246608No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT145620.15185739045557217No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC144770.15097098211957963No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC138360.14428642043285925No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC136430.14227375209348794No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT132920.13861340708250688No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT127850.1333262420666454No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA123270.1285500653856502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC117840.12288748036866244No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG115810.12077052869564492No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT108680.11333512700667205No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108130.11276156867161805No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT104700.10918465032755395No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT102990.10740140532220421No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100300.1045961836471219No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT98580.10280251030840752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG28600.017.141615
CAAGACG30350.016.5189444
AAAAGTA42850.015.326722
AAAAAGT43500.014.81
TATAACG4400.014.7159092
AGTACTC47000.014.40638355
GACGGAC33450.014.3796717
ACGGACC34050.014.1806178
AAGTACT49550.014.0383454
GCGCAAG35200.014.032671
CGCAAGA36000.013.97777752
AGACGGA35100.013.96723656
AAAGTAC47450.013.9578513
CGCTTCG20950.013.77565632
AATACCG2151.9826984E-1013.7674425
CGGACCA35200.013.5596599
AACGCTT22000.013.53863630
ACGAACG14700.013.46598715
CGAACGA14950.013.36454816
GTAAACG22750.013.33626427
Produced by FastQC (version 0.11.5)