##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727173.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9589260
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14088845228933	31.0	31.0	33.0	30.0	34.0
2	31.248474960528757	31.0	31.0	34.0	28.0	34.0
3	31.312459564137377	31.0	31.0	34.0	28.0	34.0
4	35.13480247693774	35.0	35.0	37.0	32.0	37.0
5	35.00370539541112	35.0	35.0	37.0	32.0	37.0
6	35.05251416689087	36.0	35.0	37.0	32.0	37.0
7	34.87133751718068	36.0	35.0	37.0	32.0	37.0
8	34.81124184765039	36.0	35.0	37.0	32.0	37.0
9	36.431111681193336	38.0	35.0	39.0	32.0	39.0
10	36.21662401478321	38.0	35.0	39.0	32.0	39.0
11	36.33049296817481	38.0	35.0	39.0	32.0	39.0
12	36.21802412282074	38.0	35.0	39.0	32.0	39.0
13	36.31153123390126	38.0	35.0	39.0	32.0	39.0
14	37.27463985750725	39.0	36.0	41.0	32.0	41.0
15	37.16372973514119	39.0	36.0	41.0	31.0	41.0
16	37.107599960789464	39.0	36.0	40.0	31.0	41.0
17	37.068484116605454	39.0	36.0	40.0	31.0	41.0
18	37.07357345613739	39.0	36.0	40.0	31.0	41.0
19	37.09265918329464	39.0	36.0	40.0	31.0	41.0
20	37.00449054463014	39.0	36.0	40.0	31.0	41.0
21	36.8778999630837	39.0	36.0	40.0	31.0	41.0
22	36.84536074733608	39.0	35.0	40.0	31.0	41.0
23	36.62519527054225	38.0	35.0	40.0	30.0	41.0
24	36.51279191512171	38.0	35.0	40.0	30.0	41.0
25	36.40932981272799	38.0	35.0	40.0	30.0	41.0
26	36.07610003274497	38.0	35.0	40.0	29.0	41.0
27	36.189776270535994	38.0	35.0	40.0	30.0	41.0
28	36.05899506322699	38.0	35.0	40.0	29.0	41.0
29	35.95640487378588	38.0	34.0	40.0	29.0	41.0
30	36.04105864268984	38.0	35.0	40.0	29.0	41.0
31	35.95120561962028	38.0	35.0	40.0	29.0	41.0
32	35.71196015125255	38.0	34.0	40.0	27.0	41.0
33	35.62662958351322	38.0	34.0	40.0	27.0	41.0
34	35.609204359877616	38.0	34.0	40.0	27.0	41.0
35	35.3135096973072	38.0	34.0	40.0	26.0	41.0
36	35.15191359917241	38.0	34.0	40.0	25.0	41.0
37	34.91729028100187	38.0	33.0	40.0	25.0	41.0
38	34.75199932007266	38.0	33.0	40.0	24.0	41.0
39	34.66659158266644	38.0	33.0	40.0	24.0	41.0
40	34.49809620346095	38.0	33.0	40.0	23.0	41.0
41	34.31517218221218	38.0	33.0	40.0	23.0	41.0
42	34.13411504120234	38.0	33.0	40.0	22.0	41.0
43	33.68456752658704	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	5.0
12	9.0
13	24.0
14	35.0
15	114.0
16	342.0
17	731.0
18	1471.0
19	3103.0
20	6088.0
21	11208.0
22	19640.0
23	32097.0
24	50717.0
25	75916.0
26	110038.0
27	153260.0
28	207620.0
29	268524.0
30	333663.0
31	402557.0
32	477153.0
33	564740.0
34	674320.0
35	800135.0
36	979559.0
37	1246038.0
38	1607111.0
39	1563039.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.53277520893165	19.175129259192055	12.14584858477088	25.146246947105404
2	16.60911269482734	24.98735043162872	33.17496866285824	25.228568210685705
3	21.428931950953462	26.85483551389784	29.00309304367595	22.713139491472752
4	15.24427328073282	17.05874071617622	32.66344848299035	35.03353752010061
5	13.777194486331584	38.392305558510245	33.04619960247193	14.784300352686234
6	33.29973324323253	32.64371807626449	17.265805703464085	16.790742977038896
7	26.197078815257903	31.373766067454632	23.148689262779403	19.280465854508062
8	27.716549556482985	32.08291359291541	22.71133538980067	17.48920146080094
9	28.16032728281431	13.681994231045982	20.54183534495884	37.61584314118086
10	17.973044843919133	26.938533317482268	32.750326928250985	22.338094910347618
11	33.574770107390975	22.768055094970833	21.310382657264483	22.346792140373708
12	21.556553894669662	27.552261592656784	29.619584827192085	21.271599685481466
13	30.14886445877993	19.11654288234963	26.076579423229738	24.658013235640706
14	22.747083716574583	21.94468603416739	26.615651259846956	28.69257898941107
15	27.10080861296909	25.41046962956474	24.055797840500727	23.43292391696544
16	22.48433142911966	25.615772228513983	28.961942840219162	22.93795350214719
17	24.273708294487793	26.927719135783157	27.073152672886124	21.725419896842926
18	24.34652934637292	22.799068958397207	29.897614623026175	22.956787072203692
19	23.816050456448153	27.47401780742205	28.39686274019059	20.313068995939208
20	23.131357372727404	24.53274809526491	29.3877108348298	22.948183697177885
21	22.714526459810248	26.720205730160618	28.830347701491043	21.734920108538095
22	23.537269820611808	26.451540577687954	25.648225201944676	24.362964399755562
23	20.85432035422963	26.1947220119175	28.020566759061698	24.930390874791172
24	21.59755810145934	29.253227047759683	27.134231421402692	22.014983429378283
25	23.21672370965017	25.99373674298121	25.44510212466864	25.34443742269998
26	22.71618456481522	26.347674377376357	27.840928288522786	23.095212769285638
27	24.042063725459524	25.748212062244637	26.05314695815944	24.156577254136398
28	22.05923084784436	27.05236900449044	28.287135816528075	22.601264331137127
29	22.75847145660875	25.43640489464255	28.667644844336266	23.13747880441244
30	21.90724831738841	27.966725273900177	29.153521752460566	20.972504656250848
31	24.754318894262955	25.815631237446894	25.710065218796863	23.719984649493288
32	20.087441575262325	27.37418737212256	27.921685302098386	24.616685750516723
33	21.62726842321514	26.634401403236534	28.52660163557981	23.21172853796852
34	21.450018041016722	26.4236447859376	28.435833421974166	23.69050375107151
35	21.553289826326534	27.93101866045972	29.100003545633346	21.415687967580396
36	22.769285638307856	26.688524453398905	27.35029606038422	23.191893847909014
37	21.847754675543264	27.063297897856557	28.31001557993005	22.77893184667013
38	22.44318122566288	26.386300924158903	28.58189265907901	22.58862519109921
39	22.788765765032963	24.353860464728246	28.027824879083475	24.829548891155316
40	22.260414255114576	26.123652920037628	28.617985120853955	22.997947703993844
41	20.762165172286494	26.087028613261086	28.94983554518284	24.200970669269577
42	22.557778181006668	26.89421290068264	28.345555340036665	22.202453578274028
43	21.501169016170174	24.575837968727516	29.05126151548712	24.871731499615194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2772.0
1	3305.0
2	3838.0
3	13671.0
4	23504.0
5	23504.0
6	34325.0
7	45146.0
8	44902.5
9	44659.0
10	53240.0
11	61821.0
12	61821.0
13	88725.0
14	115629.0
15	150206.5
16	184784.0
17	164649.0
18	144514.0
19	144514.0
20	143539.5
21	142565.0
22	120135.0
23	97705.0
24	102200.0
25	106695.0
26	106695.0
27	114260.0
28	121825.0
29	128585.0
30	135345.0
31	147971.5
32	160598.0
33	160598.0
34	186192.0
35	211786.0
36	253318.0
37	294850.0
38	338471.5
39	382093.0
40	382093.0
41	413992.0
42	445891.0
43	487287.0
44	528683.0
45	619454.0
46	710225.0
47	710225.0
48	997452.5
49	1284680.0
50	1285074.0
51	1285468.0
52	975157.5
53	664847.0
54	664847.0
55	605889.0
56	546931.0
57	523059.0
58	499187.0
59	465983.5
60	432780.0
61	432780.0
62	360998.5
63	289217.0
64	251436.5
65	213656.0
66	180556.5
67	147457.0
68	147457.0
69	123189.5
70	98922.0
71	84771.5
72	70621.0
73	52473.5
74	34326.0
75	34326.0
76	26881.0
77	19436.0
78	16774.0
79	14112.0
80	11513.0
81	8914.0
82	8914.0
83	7506.0
84	6098.0
85	5341.0
86	4584.0
87	3106.5
88	1629.0
89	1629.0
90	1183.5
91	738.0
92	537.0
93	336.0
94	342.5
95	349.0
96	349.0
97	184.5
98	20.0
99	22.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9589260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.339800331487695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59674892294149	28.129332139723108
2	9.881837519370608	6.984443296855219
3	3.6133912867530564	3.830895797801713
4	1.7881360154168076	2.527694790014879
5	1.0397650227636148	1.8372544148065457
6	0.7045024003236657	1.4938184496295295
7	0.5070854168470518	1.2544208167668813
8	0.36826117905583683	1.041142123013721
9	0.3015157985208892	0.9589957304865568
>10	1.8940975506645352	12.741177097920763
>50	0.1635700071649022	3.955572492697198
>100	0.10692939357802018	7.660931204506248
>500	0.016735192656472803	4.138981318614687
>1k	0.01496509927890662	10.31857435766982
>5k	0.0013795482262639728	3.300013596207209
>10k+	0.0010796464379457177	9.826752373286114
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	80735	0.8419314941924612	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	72764	0.7588072489430884	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	67486	0.7037665054446329	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	52282	0.5452141249689757	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	44291	0.46188131305231067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39868	0.41575679458060366	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	37679	0.3929291728454542	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	36999	0.38583790615751373	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	36033	0.3757641361272924	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33811	0.3525923793911105	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	31396	0.3274079543155572	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	29946	0.3122868709368606	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	24325	0.253669209094341	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	23401	0.24403342906543363	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	21103	0.2200691189935407	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	20822	0.21713875731808296	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	19665	0.2050731756152195	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	19000	0.19813833392774835	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	18388	0.1917561939086019	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15979	0.16663433883323636	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	15344	0.1600123471467037	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	15102	0.15748869047246608	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	14562	0.15185739045557217	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	14477	0.15097098211957963	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	13836	0.14428642043285925	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	13643	0.14227375209348794	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	13292	0.13861340708250688	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	12785	0.1333262420666454	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	12327	0.1285500653856502	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	11784	0.12288748036866244	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	11581	0.12077052869564492	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	10868	0.11333512700667205	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10813	0.11276156867161805	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	10470	0.10918465032755395	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	10299	0.10740140532220421	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10030	0.1045961836471219	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	9858	0.10280251030840752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0856666729236668E-5
2	0.0	0.0	0.0	0.0	2.0856666729236668E-5
3	1.0428333364618334E-5	0.0	0.0	0.0	2.0856666729236668E-5
4	1.0428333364618334E-5	0.0	0.0	0.0	2.0856666729236668E-5
5	1.0428333364618334E-5	0.0	0.0	1.0428333364618334E-5	2.0856666729236668E-5
6	1.0428333364618334E-5	0.0	0.0	1.0428333364618334E-5	3.1285000093855E-5
7	1.0428333364618334E-5	1.0428333364618334E-5	0.0	1.0428333364618334E-5	3.1285000093855E-5
8	2.0856666729236668E-5	1.0428333364618334E-5	0.0	1.0428333364618334E-5	4.1713333458473337E-5
9	2.0856666729236668E-5	1.0428333364618334E-5	0.0	1.0428333364618334E-5	4.1713333458473337E-5
10	2.0856666729236668E-5	1.0428333364618334E-5	0.0	1.3556833374003835E-4	4.1713333458473337E-5
11	2.0856666729236668E-5	1.0428333364618334E-5	0.0	3.337066676677867E-4	4.1713333458473337E-5
12	3.1285000093855E-5	1.0428333364618334E-5	0.0	6.257000018771E-4	5.2141666823091666E-5
13	3.1285000093855E-5	1.0428333364618334E-5	0.0	8.864083359925583E-4	6.257000018771E-5
14	4.1713333458473337E-5	1.0428333364618334E-5	0.0	0.0016372483382450784	8.342666691694667E-5
15	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.002721795008165385	1.0428333364618333E-4
16	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.006017148351384778	1.2514000037542E-4
17	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.010042485030127455	1.2514000037542E-4
18	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.012514000037542	1.2514000037542E-4
19	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.018124443387706662	1.2514000037542E-4
20	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.022869335068608006	1.3556833374003835E-4
21	5.2141666823091666E-5	1.0428333364618334E-5	0.0	0.034695065104085196	1.3556833374003835E-4
22	5.2141666823091666E-5	2.0856666729236668E-5	0.0	0.05206866848953934	1.3556833374003835E-4
23	6.257000018771E-5	2.0856666729236668E-5	0.0	0.07754508689930192	1.6685333383389335E-4
24	6.257000018771E-5	2.0856666729236668E-5	0.0	0.11757945868607171	1.6685333383389335E-4
25	6.257000018771E-5	2.0856666729236668E-5	0.0	0.14217989709320636	1.7728166719851166E-4
26	6.257000018771E-5	2.0856666729236668E-5	0.0	0.1805561638750018	1.7728166719851166E-4
27	6.257000018771E-5	2.0856666729236668E-5	0.0	0.25963421577890267	1.7728166719851166E-4
28	6.257000018771E-5	2.0856666729236668E-5	0.0	0.3942952845162192	1.8771000056313E-4
29	6.257000018771E-5	2.0856666729236668E-5	0.0	0.5041160631790148	1.8771000056313E-4
30	6.257000018771E-5	2.0856666729236668E-5	0.0	0.6696449986756017	1.8771000056313E-4
31	6.257000018771E-5	2.0856666729236668E-5	0.0	0.9872503196284176	2.0856666729236666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2860	0.0	17.14161	5
CAAGACG	3035	0.0	16.518944	4
AAAAGTA	4285	0.0	15.32672	2
AAAAAGT	4350	0.0	14.8	1
TATAACG	440	0.0	14.715909	2
AGTACTC	4700	0.0	14.4063835	5
GACGGAC	3345	0.0	14.379671	7
ACGGACC	3405	0.0	14.180617	8
AAGTACT	4955	0.0	14.038345	4
GCGCAAG	3520	0.0	14.03267	1
CGCAAGA	3600	0.0	13.9777775	2
AGACGGA	3510	0.0	13.9672365	6
AAAGTAC	4745	0.0	13.957851	3
CGCTTCG	2095	0.0	13.775656	32
AATACCG	215	1.9826984E-10	13.767442	5
CGGACCA	3520	0.0	13.559659	9
AACGCTT	2200	0.0	13.538636	30
ACGAACG	1470	0.0	13.465987	15
CGAACGA	1495	0.0	13.364548	16
GTAAACG	2275	0.0	13.336264	27
>>END_MODULE