FastQCFastQC Report
Thu 9 Feb 2017
SRR2727172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361296
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT416730.7772934007001292No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC338770.631880798970995No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG312270.5824524517952375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT273360.5098767163760404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243460.4541066189966008No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA218620.40777453809675873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203730.3800014026459274No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG169750.3166212050220693No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG159550.29759595441102304No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT137040.25560983762135125No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC129210.24100515994640104No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG128190.2391026348852964No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT107540.20058582850116835No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT98320.18338849412530106No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA95720.1785389204401324No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC93450.17430486956885052No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG91700.17104073343460238No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT85650.15975614851334452No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82790.15442161745965902No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC78870.14710995251894318No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC68350.12748783130049152No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT66030.12316051939680256No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT64260.1198590788495916No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT63860.11911299059033488No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT62520.11661359492182487No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC61130.11402093822090778No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC60060.11202515212739607No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC59940.11180132564961906No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.10469483498019881No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT55890.1042471820246448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG450.003827609620.5555553
AAGACGG15200.016.18755
CGTTACG8500.015.45294224
CAAGACG16400.015.1158544
GTTACGG14750.014.42372925
TACGGAT18150.014.37190127
ACGGATG18800.014.26861828
TCGTTAC10850.014.15207423
TATACCG1702.442539E-814.14705855
GCGCAAG18200.013.8241771
GTAATAC2950.013.796613
CGCAAGA18700.013.7513362
TACGCAG1356.5772547E-613.7037035
GACGGAC18050.013.6315787
TACCGTC11700.013.598297
AGTACTC20750.013.5518075
ACCGTCG11000.013.4545458
ACGGACC18100.013.3895038
CGGATGG20250.013.33827229
TTACGGA19700.013.241117526
Produced by FastQC (version 0.11.5)