##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5361296
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137263452717402	31.0	31.0	33.0	30.0	34.0
2	31.26226998098967	31.0	31.0	34.0	30.0	34.0
3	31.316722113459132	31.0	31.0	34.0	28.0	34.0
4	35.151464869688226	35.0	35.0	37.0	33.0	37.0
5	35.01623170964633	35.0	35.0	37.0	32.0	37.0
6	35.05495611508859	36.0	35.0	37.0	32.0	37.0
7	34.87363242022078	36.0	35.0	37.0	32.0	37.0
8	34.808919708965895	36.0	35.0	37.0	32.0	37.0
9	36.415845161319204	38.0	35.0	39.0	32.0	39.0
10	36.21117282090002	38.0	35.0	39.0	32.0	39.0
11	36.325945629564195	38.0	35.0	39.0	32.0	39.0
12	36.20490493343401	38.0	35.0	39.0	32.0	39.0
13	36.30199265252282	38.0	35.0	39.0	32.0	39.0
14	37.24915524156846	39.0	36.0	41.0	32.0	41.0
15	37.14553067019616	39.0	36.0	41.0	31.0	41.0
16	37.10439621315443	39.0	36.0	40.0	31.0	41.0
17	37.05499341950156	39.0	36.0	40.0	31.0	41.0
18	37.06944104559793	39.0	36.0	40.0	31.0	41.0
19	37.1044366884425	39.0	36.0	40.0	31.0	41.0
20	36.999805457486396	39.0	36.0	40.0	31.0	41.0
21	36.88398085089874	39.0	36.0	40.0	31.0	41.0
22	36.844584779501076	39.0	35.0	40.0	31.0	41.0
23	36.629578930169124	38.0	35.0	40.0	30.0	41.0
24	36.52062989993464	38.0	35.0	40.0	30.0	41.0
25	36.412084316926354	38.0	35.0	40.0	30.0	41.0
26	36.09599395370075	38.0	35.0	40.0	29.0	41.0
27	36.207225640964424	38.0	35.0	40.0	30.0	41.0
28	36.08215979867554	38.0	35.0	40.0	29.0	41.0
29	35.97675506071666	38.0	34.0	40.0	29.0	41.0
30	36.0755761293538	38.0	35.0	40.0	29.0	41.0
31	35.984730371163984	38.0	35.0	40.0	29.0	41.0
32	35.781368348250126	38.0	34.0	40.0	28.0	41.0
33	35.69228056052119	38.0	34.0	40.0	27.0	41.0
34	35.69208079538977	38.0	34.0	40.0	27.0	41.0
35	35.40903747899762	38.0	34.0	40.0	26.0	41.0
36	35.22977280120329	38.0	34.0	40.0	25.0	41.0
37	35.01382277717925	38.0	33.0	40.0	25.0	41.0
38	34.848411839226934	38.0	33.0	40.0	24.0	41.0
39	34.754387558530624	38.0	33.0	40.0	24.0	41.0
40	34.61446728552201	38.0	33.0	40.0	24.0	41.0
41	34.42830763307976	38.0	33.0	40.0	23.0	41.0
42	34.256321046254484	38.0	33.0	40.0	23.0	41.0
43	33.81614147027137	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	9.0
13	8.0
14	18.0
15	67.0
16	195.0
17	410.0
18	788.0
19	1623.0
20	3244.0
21	5826.0
22	10381.0
23	17466.0
24	27411.0
25	41139.0
26	59710.0
27	83225.0
28	113516.0
29	147961.0
30	184632.0
31	224744.0
32	267322.0
33	316906.0
34	379661.0
35	452136.0
36	548362.0
37	694573.0
38	885879.0
39	894081.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.77941378353294	19.404356707781105	12.812051414434123	25.004178094251838
2	16.986228702910637	24.58642462568752	33.7562223760822	24.67112429531964
3	21.164546781226033	26.167348342639542	29.611608834878734	23.0564960412557
4	15.365501177327273	16.876460467767494	32.85019144624732	34.90784690865791
5	13.926725925970137	38.62457883317765	32.82310844243631	14.625586798415904
6	33.4988592310516	33.188952820362836	16.864000793837906	16.44818715474766
7	26.424170573682183	31.97795831455678	22.56997188739439	19.027899224366646
8	27.826835153291295	32.57505274843993	22.03985752698601	17.558254571282763
9	28.556509470844365	13.6973037862487	20.205730107048744	37.54045663585819
10	17.82141855252909	27.337028211089258	32.54668274238169	22.29487049399996
11	34.15006371593734	22.804411470659332	20.90444176184266	22.141083051560667
12	21.625405498968906	27.266821305893203	29.438646924176542	21.66912627096135
13	30.330334307227208	19.336798415905406	25.83302619366661	24.499841083200778
14	22.702346596793014	22.106091512201527	26.403914277443363	28.787647613562093
15	26.991570694846917	26.137952465224828	23.775892993037502	23.09458384689075
16	22.549118720548165	25.976816053431858	28.191635007654863	23.28243021836511
17	23.88474727006306	27.001400407662622	26.720293003781176	22.39355931849314
18	23.869396504128854	23.696621115491478	29.542782192962296	22.891200187417372
19	23.730120478332104	27.255163676842315	28.429264118228133	20.58545172659745
20	23.22910356003474	24.6348271015068	28.883874346799733	23.252194991658733
21	22.93210447623112	26.776212318812465	28.641171836063517	21.650511368892893
22	23.657358220848092	26.157835717334017	25.82700153097311	24.35780453084478
23	21.18041980894172	26.166415732315468	28.13088477114489	24.522279687597926
24	21.835205517471895	28.571393931616534	27.22449944938686	22.368901101524706
25	23.304682300697444	25.790685684953786	25.75457501320576	25.150057001143004
26	22.635814176273797	26.501409360721734	27.8955871863818	22.96718927662267
27	23.996623204538604	26.12116547939155	26.177644360617286	23.704566955452563
28	21.95144606826409	27.245986791253458	27.989202610712038	22.813364529770414
29	22.7555613418845	25.967993559766146	28.531403600920374	22.745041497428982
30	22.473316153407684	27.73704343128975	28.47192171445113	21.317718700851433
31	24.266408719085835	26.216776689815298	26.10986224226381	23.40695234883506
32	20.630459500837112	27.493128527132242	27.672973102026077	24.203438870004565
33	21.699585324145502	26.558802200065056	28.33798021970807	23.403632256081366
34	21.830934162187653	26.643930870446255	28.36648079121168	23.15865417615442
35	21.904666334408695	27.7110982120741	28.78455134728618	21.59968410623103
36	22.590563923349876	26.83438855082801	27.56436130368478	23.010686222137334
37	21.694884968112188	27.14759640206398	28.507379558972307	22.650139070851523
38	22.480422644077102	26.229758625526365	28.549552197826795	22.740266532569738
39	22.33670739313778	24.76162480116748	28.47531641603075	24.426351389663992
40	22.28953596294627	26.014903859066912	28.885515740970092	22.81004443701672
41	20.789973170666197	26.093485604973125	29.275346856431728	23.841194367928946
42	22.26277004664544	26.775932535715246	28.78270477884452	22.1785926387948
43	21.065932565558775	24.9634603275029	29.25066998725681	24.71993711968151
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3051.0
1	3295.5
2	3540.0
3	7651.5
4	11763.0
5	11763.0
6	17267.5
7	22772.0
8	22510.0
9	22248.0
10	27190.0
11	32132.0
12	32132.0
13	48308.5
14	64485.0
15	84879.0
16	105273.0
17	93213.5
18	81154.0
19	81154.0
20	80320.0
21	79486.0
22	66362.0
23	53238.0
24	57498.0
25	61758.0
26	61758.0
27	66447.0
28	71136.0
29	75267.0
30	79398.0
31	86406.0
32	93414.0
33	93414.0
34	108882.0
35	124350.0
36	148290.5
37	172231.0
38	197527.5
39	222824.0
40	222824.0
41	241288.5
42	259753.0
43	282452.5
44	305152.0
45	352649.5
46	400147.0
47	400147.0
48	533783.5
49	667420.0
50	666249.0
51	665078.0
52	526975.5
53	388873.0
54	388873.0
55	356250.0
56	323627.0
57	309406.5
58	295186.0
59	271295.5
60	247405.0
61	247405.0
62	204988.0
63	162571.0
64	140244.0
65	117917.0
66	99538.0
67	81159.0
68	81159.0
69	67624.0
70	54089.0
71	46452.5
72	38816.0
73	28713.0
74	18610.0
75	18610.0
76	14619.0
77	10628.0
78	9348.0
79	8068.0
80	6472.5
81	4877.0
82	4877.0
83	4161.5
84	3446.0
85	3021.0
86	2596.0
87	1717.5
88	839.0
89	839.0
90	615.5
91	392.0
92	294.5
93	197.0
94	184.5
95	172.0
96	172.0
97	92.0
98	12.0
99	12.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5361296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.762077577095205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.75965918451672	34.14453229540652
2	9.177222135427181	7.6651972552389065
3	3.110890676467085	3.8975177329344177
4	1.5729631978143273	2.6276084437215075
5	0.9243819586044049	1.9302055533052185
6	0.6359456158262777	1.5935046085770361
7	0.4613379628805262	1.3486502256552926
8	0.34683522716797116	1.1587647730766604
9	0.2608128028916139	0.9802876056713299
>10	1.5103763895199596	11.493981356853157
>50	0.11668808736995649	3.3500055342464212
>100	0.09455995671479413	8.389358573838637
>500	0.015504720226586029	4.546844618901138
>1k	0.011230691097362891	8.63014053099188
>5k	0.001000304470210443	2.8540348614049535
>10k+	5.910890051243528E-4	5.3893660301768875
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41673	0.7772934007001292	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	33877	0.631880798970995	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	31227	0.5824524517952375	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27336	0.5098767163760404	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24346	0.4541066189966008	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	21862	0.40777453809675873	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20373	0.3800014026459274	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	16975	0.3166212050220693	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	15955	0.29759595441102304	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	13704	0.25560983762135125	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	12921	0.24100515994640104	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	12819	0.2391026348852964	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	10754	0.20058582850116835	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	9832	0.18338849412530106	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	9572	0.1785389204401324	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	9345	0.17430486956885052	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	9170	0.17104073343460238	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	8565	0.15975614851334452	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8279	0.15442161745965902	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	7887	0.14710995251894318	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	6835	0.12748783130049152	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	6603	0.12316051939680256	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	6426	0.1198590788495916	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	6386	0.11911299059033488	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	6252	0.11661359492182487	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	6113	0.11402093822090778	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	6006	0.11202515212739607	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	5994	0.11180132564961906	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5613	0.10469483498019881	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5589	0.1042471820246448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.730441296283585E-5
5	0.0	0.0	0.0	0.0	3.730441296283585E-5
6	1.8652206481417925E-5	0.0	0.0	0.0	9.326103240708963E-5
7	3.730441296283585E-5	0.0	0.0	0.0	9.326103240708963E-5
8	3.730441296283585E-5	0.0	0.0	0.0	1.3056544536992548E-4
9	5.5956619444253776E-5	0.0	0.0	1.8652206481417925E-5	1.6786985833276133E-4
10	5.5956619444253776E-5	0.0	0.0	1.8652206481417925E-5	1.6786985833276133E-4
11	5.5956619444253776E-5	0.0	0.0	1.492176518513434E-4	1.6786985833276133E-4
12	5.5956619444253776E-5	0.0	0.0	4.290007490726123E-4	1.8652206481417925E-4
13	5.5956619444253776E-5	0.0	0.0	6.155228138867916E-4	1.8652206481417925E-4
14	5.5956619444253776E-5	1.8652206481417925E-5	0.0	0.0012310456277735832	2.4247868425843303E-4
15	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.0024620912555471663	2.797830972212689E-4
16	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.005632966357388213	2.984353037026868E-4
17	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.00899036352404344	2.984353037026868E-4
18	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.010930192998110905	3.1708751018410473E-4
19	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.015499983586058296	3.1708751018410473E-4
20	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.01978999107678442	3.1708751018410473E-4
21	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.02889226783971637	3.1708751018410473E-4
22	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.042116682235041676	3.1708751018410473E-4
23	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.05979897397942587	3.730441296283585E-4
24	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.08912024256821485	3.730441296283585E-4
25	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.10646679459593351	3.730441296283585E-4
26	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.13595593304305525	3.730441296283585E-4
27	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.2044841396557847	3.9169633610977643E-4
28	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.3196055580590962	3.9169633610977643E-4
29	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.4254008732216986	3.9169633610977643E-4
30	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.5892978115739179	3.9169633610977643E-4
31	7.46088259256717E-5	1.8652206481417925E-5	0.0	0.9552727549458191	3.9169633610977643E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAACG	45	0.0038276096	20.555555	3
AAGACGG	1520	0.0	16.1875	5
CGTTACG	850	0.0	15.452942	24
CAAGACG	1640	0.0	15.115854	4
GTTACGG	1475	0.0	14.423729	25
TACGGAT	1815	0.0	14.371901	27
ACGGATG	1880	0.0	14.268618	28
TCGTTAC	1085	0.0	14.152074	23
TATACCG	170	2.442539E-8	14.1470585	5
GCGCAAG	1820	0.0	13.824177	1
GTAATAC	295	0.0	13.79661	3
CGCAAGA	1870	0.0	13.751336	2
TACGCAG	135	6.5772547E-6	13.703703	5
GACGGAC	1805	0.0	13.631578	7
TACCGTC	1170	0.0	13.59829	7
AGTACTC	2075	0.0	13.551807	5
ACCGTCG	1100	0.0	13.454545	8
ACGGACC	1810	0.0	13.389503	8
CGGATGG	2025	0.0	13.338272	29
TTACGGA	1970	0.0	13.2411175	26
>>END_MODULE