FastQCFastQC Report
Thu 9 Feb 2017
SRR2727170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9385536
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC739760.7881915321618286No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG722270.7695564749844868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT550250.5862744546502192No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA466240.4967643829824956No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG395130.42099886463596753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT361850.3855400480057825No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG334870.35679368764873953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT332430.3541939426794591No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC289260.3081976351696909No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT286180.30491598988059926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269170.2867923579431159No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG265240.2826050637917749No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT232930.24817975233380382No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC230200.24527102128210895No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG213900.22790387251191618No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT213680.2276694692769811No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA212840.2267744751072288No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC187000.1992427496948496No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT186520.19873132445499117No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT147220.15685838294158158No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT142890.15224490109035863No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC141490.15075324414077151No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC141360.15061473313830984No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC138680.14775927554910023No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT134230.14301793738791263No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT133850.1426130590730247No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC131760.1403862283411411No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123370.1314469413361155No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA119620.12745143164972145No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT117650.12535245722780244No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT116320.12393538312569469No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG112400.11975874366685077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC106910.11390931748596991No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT102040.10872048223990616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT101580.10823036638504184No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG100370.10694114859289869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG30650.017.0815665
CAAGACG31550.016.8288424
TCTATAC6450.015.20155053
TAATACG1651.6496415E-814.5757594
AAAAAGT36500.014.0904121
TATAACG3550.014.0704222
TACCGTC25400.013.9842527
CGCAAGA37250.013.9557052
AGTACTC39300.013.9338435
AAAAGTA37450.013.8811742
ACCGTCG25350.013.8658788
AAGTACT41400.013.7185994
CGAACGT13600.013.60294154
GACGGAC37850.013.5878477
ATACCGT26300.013.5760456
CTTATAC86750.013.54178637
TGGGCTG67000.013.41940336
GCGCAAG38250.013.3973871
CGGTCCA22000.013.28636510
TATCACG3500.013.2142852
Produced by FastQC (version 0.11.5)