##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9385536
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14878628135889	31.0	31.0	33.0	30.0	34.0
2	31.26639629318986	31.0	31.0	34.0	30.0	34.0
3	31.32476216595408	31.0	31.0	34.0	28.0	34.0
4	35.156028702036835	35.0	35.0	37.0	33.0	37.0
5	35.01565078435584	35.0	35.0	37.0	32.0	37.0
6	35.05756666428002	36.0	35.0	37.0	32.0	37.0
7	34.86854325634679	36.0	35.0	37.0	32.0	37.0
8	34.80603750281284	36.0	35.0	37.0	32.0	37.0
9	36.42923025387149	38.0	35.0	39.0	32.0	39.0
10	36.21718056379518	38.0	35.0	39.0	32.0	39.0
11	36.33036866514603	38.0	35.0	39.0	32.0	39.0
12	36.2077209016086	38.0	35.0	39.0	32.0	39.0
13	36.30198222030154	38.0	35.0	39.0	32.0	39.0
14	37.26441153707151	39.0	36.0	41.0	32.0	41.0
15	37.15233589216428	39.0	36.0	41.0	31.0	41.0
16	37.09880831526298	39.0	36.0	40.0	31.0	41.0
17	37.04232214334908	39.0	36.0	40.0	31.0	41.0
18	37.03905051347094	39.0	36.0	40.0	31.0	41.0
19	37.055651802944446	39.0	36.0	40.0	31.0	41.0
20	36.96292742364421	39.0	36.0	40.0	31.0	41.0
21	36.83275169366992	39.0	36.0	40.0	30.0	41.0
22	36.80023900606209	39.0	35.0	40.0	30.0	41.0
23	36.58614798345028	38.0	35.0	40.0	30.0	41.0
24	36.46455460828236	38.0	35.0	40.0	30.0	41.0
25	36.35571074470334	38.0	35.0	40.0	30.0	41.0
26	36.02760300530518	38.0	34.0	40.0	29.0	41.0
27	36.14955789418953	38.0	35.0	40.0	29.0	41.0
28	36.01460747686653	38.0	35.0	40.0	29.0	41.0
29	35.913243313967364	38.0	34.0	40.0	28.0	41.0
30	36.015327840626256	38.0	35.0	40.0	29.0	41.0
31	35.941011786647024	38.0	35.0	40.0	29.0	41.0
32	35.72141196837347	38.0	34.0	40.0	27.0	41.0
33	35.646824326282484	38.0	34.0	40.0	27.0	41.0
34	35.646432233598595	38.0	34.0	40.0	27.0	41.0
35	35.35764116188996	38.0	34.0	40.0	26.0	41.0
36	35.21011138841724	38.0	34.0	40.0	25.0	41.0
37	34.99143256176312	38.0	33.0	40.0	25.0	41.0
38	34.830621714092835	38.0	33.0	40.0	24.0	41.0
39	34.761077257601485	38.0	33.0	40.0	24.0	41.0
40	34.60752108350551	38.0	33.0	40.0	24.0	41.0
41	34.43415666404135	38.0	33.0	40.0	23.0	41.0
42	34.25387084978418	38.0	33.0	40.0	23.0	41.0
43	33.81352157191662	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	10.0
13	18.0
14	30.0
15	118.0
16	406.0
17	810.0
18	1568.0
19	3096.0
20	6232.0
21	11255.0
22	19477.0
23	31837.0
24	49769.0
25	75151.0
26	107746.0
27	149309.0
28	200233.0
29	259686.0
30	323655.0
31	392949.0
32	464862.0
33	554230.0
34	662754.0
35	787629.0
36	957173.0
37	1208088.0
38	1568219.0
39	1549222.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.89979815750534	19.216462437520885	12.395903654303813	25.48783575066997
2	16.97351115588923	24.51022509529557	32.98784427442396	25.528419474391235
3	21.217999696554358	26.08934641559097	29.10654223690581	23.586111650948865
4	15.443252255385309	17.082125091204166	32.59956597044644	34.875056682964086
5	14.088827745160213	37.77104472243247	33.247243417957165	14.89288411445015
6	33.84489708419423	32.97137211982352	16.777624634330955	16.40610616165129
7	26.520616403794094	31.205324874359867	22.926937790233822	19.347120931612217
8	27.706696772565785	32.08788501796807	22.32016370721928	17.885254502246863
9	27.920579069751582	13.79884963416048	20.09649741900729	38.18407387708065
10	17.95354042646046	26.678827932682804	32.578160693219864	22.789470947636875
11	34.39828050310606	22.405582376968137	20.80010134743503	22.396035772490777
12	21.804753612366945	27.110545417970798	29.120862143621846	21.963838826040412
13	30.672707451124793	18.791297588118567	25.39544891202804	25.140546048728595
14	22.918371417466194	21.887743012226473	25.71055078793582	29.48333478237151
15	27.434948840428508	25.465087982188763	23.320074633990004	23.779888543392726
16	23.321481053399616	25.327706377131793	27.90719677597529	23.443615793493308
17	24.62315418107181	26.778555854455195	26.41162955424176	22.186660410231234
18	25.094017006593976	22.51823444073945	29.035358236333014	23.352390316333558
19	24.19916134784417	27.493837325859705	27.387279746196704	20.91972158009942
20	23.811788692728896	24.195059291232806	28.257086222885942	23.73606579315236
21	23.528352562922354	26.61835189806954	27.36142080750636	22.49187473150175
22	23.93927208845611	26.191386405635225	24.858793360336588	25.01054814557208
23	21.380537030596866	25.5755025605357	27.047746660393184	25.99621374847425
24	22.355484012847	28.884189459184856	26.132135660659124	22.628190867309016
25	24.004148511070653	25.28125191784465	24.545833077620713	26.168766493463984
26	23.341106996979182	26.05068053651917	27.024370265054653	23.58384220144699
27	24.954674938117545	25.25521184938186	25.14245323868557	24.647659973815028
28	22.70888950828168	26.28960136107304	27.58429566515967	23.417213465485613
29	23.454398342300323	25.093814567436535	27.82005204604191	23.631735044221237
30	22.563378372849456	27.42056500555749	28.27460253735109	21.741454084241965
31	25.276137665446065	25.454134958301793	24.686997098514137	24.582730277737998
32	20.82720688514753	26.80896434684178	26.954496791658993	25.409331976351694
33	22.36116296394793	25.98272490777298	27.588291174846063	24.067820953433028
34	22.427509734127067	25.8916166322307	27.498408188940942	24.182465444701293
35	22.608362484572005	27.081042574446467	28.396705313367292	21.913889627614235
36	23.55567119448479	26.16306623297806	26.609465884527	23.671796688010147
37	22.643331185347325	26.306915236380746	27.611028288634763	23.438725289637162
38	23.139424322702506	25.548951066833048	28.0104833650417	23.301141245422745
39	23.460791157798553	23.473832501414943	27.45511817332542	25.61025816746108
40	22.939467708610355	25.337242326916652	28.24943615367306	23.473853810799937
41	21.470867513586864	25.153885723734902	28.709452502233223	24.665794260445008
42	23.438703980252168	25.901536150945454	28.103914363548338	22.55584550525404
43	22.200064013392524	23.692402863299442	28.812515342757195	25.29501778055084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2668.0
1	2963.0
2	3258.0
3	10287.0
4	17316.0
5	17316.0
6	25677.5
7	34039.0
8	33691.5
9	33344.0
10	40359.5
11	47375.0
12	47375.0
13	68965.5
14	90556.0
15	119429.0
16	148302.0
17	133636.0
18	118970.0
19	118970.0
20	119007.0
21	119044.0
22	99743.5
23	80443.0
24	85030.0
25	89617.0
26	89617.0
27	96852.0
28	104087.0
29	110704.0
30	117321.0
31	130008.5
32	142696.0
33	142696.0
34	170735.0
35	198774.0
36	244667.5
37	290561.0
38	334907.5
39	379254.0
40	379254.0
41	410473.5
42	441693.0
43	480771.0
44	519849.0
45	604845.0
46	689841.0
47	689841.0
48	974588.5
49	1259336.0
50	1248956.0
51	1238576.0
52	949297.5
53	660019.0
54	660019.0
55	609761.0
56	559503.0
57	542380.0
58	525257.0
59	492207.5
60	459158.0
61	459158.0
62	380382.0
63	301606.0
64	265390.0
65	229174.0
66	196681.0
67	164188.0
68	164188.0
69	139724.5
70	115261.0
71	100932.0
72	86603.0
73	64560.5
74	42518.0
75	42518.0
76	33595.5
77	24673.0
78	21759.5
79	18846.0
80	15718.5
81	12591.0
82	12591.0
83	10685.5
84	8780.0
85	7530.5
86	6281.0
87	4282.0
88	2283.0
89	2283.0
90	1647.0
91	1011.0
92	724.0
93	437.0
94	412.0
95	387.0
96	387.0
97	202.5
98	18.0
99	20.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9385536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.81695399728903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32126751836866	27.61724922193698
2	10.249038150672906	7.136805796168777
3	3.6204748002559537	3.7816171370656715
4	1.8037068615601788	2.5119831529414127
5	1.0261914971185992	1.7864431073793718
6	0.6833771181276539	1.4275865810790287
7	0.49428875597725996	1.204674021476624
8	0.37598660943117396	1.0472566787329491
9	0.28179871111511606	0.8830235485251303
>10	1.8569082123593261	12.1778512063005
>50	0.1463624310020208	3.5143704296747984
>100	0.10309031240500058	7.3580379729306795
>500	0.017761126917408904	4.327268221632144
>1k	0.016860230845752344	11.798372255126962
>5k	0.0017698584843639973	4.128666509511674
>10k+	0.0011178053585456824	9.298794159517229
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	73976	0.7881915321618286	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	72227	0.7695564749844868	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	55025	0.5862744546502192	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	46624	0.4967643829824956	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	39513	0.42099886463596753	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36185	0.3855400480057825	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	33487	0.35679368764873953	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33243	0.3541939426794591	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	28926	0.3081976351696909	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	28618	0.30491598988059926	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26917	0.2867923579431159	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	26524	0.2826050637917749	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	23293	0.24817975233380382	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	23020	0.24527102128210895	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	21390	0.22790387251191618	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	21368	0.2276694692769811	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	21284	0.2267744751072288	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	18700	0.1992427496948496	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	18652	0.19873132445499117	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	14722	0.15685838294158158	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	14289	0.15224490109035863	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	14149	0.15075324414077151	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	14136	0.15061473313830984	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	13868	0.14775927554910023	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	13423	0.14301793738791263	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	13385	0.1426130590730247	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	13176	0.1403862283411411	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12337	0.1314469413361155	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	11962	0.12745143164972145	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	11765	0.12535245722780244	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	11632	0.12393538312569469	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	11240	0.11975874366685077	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	10691	0.11390931748596991	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	10204	0.10872048223990616	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	10158	0.10823036638504184	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	10037	0.10694114859289869	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0654692497050781E-5
2	0.0	0.0	0.0	0.0	1.0654692497050781E-5
3	0.0	0.0	0.0	0.0	1.0654692497050781E-5
4	0.0	0.0	0.0	0.0	3.1964077491152344E-5
5	0.0	0.0	0.0	0.0	3.1964077491152344E-5
6	0.0	0.0	0.0	0.0	4.2618769988203125E-5
7	0.0	0.0	0.0	0.0	4.2618769988203125E-5
8	2.1309384994101563E-5	0.0	0.0	0.0	5.327346248525391E-5
9	2.1309384994101563E-5	0.0	0.0	2.1309384994101563E-5	1.0654692497050781E-4
10	2.1309384994101563E-5	0.0	0.0	5.327346248525391E-5	1.0654692497050781E-4
11	2.1309384994101563E-5	0.0	0.0	1.2785630996460938E-4	1.0654692497050781E-4
12	3.1964077491152344E-5	1.0654692497050781E-5	0.0	2.9833138991742185E-4	1.0654692497050781E-4
13	3.1964077491152344E-5	2.1309384994101563E-5	0.0	4.474970848761328E-4	1.0654692497050781E-4
14	3.1964077491152344E-5	3.1964077491152344E-5	0.0	6.712456273141992E-4	1.2785630996460938E-4
15	5.327346248525391E-5	3.1964077491152344E-5	0.0	0.0012252896371608398	1.4916569495871093E-4
16	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.003015277976665371	1.8112977244986328E-4
17	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.005060978936099121	1.8112977244986328E-4
18	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.006595254655674433	2.0243915744396485E-4
19	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.010121957872198242	2.0243915744396485E-4
20	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.013115926463869512	2.0243915744396485E-4
21	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.020542247134313907	2.1309384994101563E-4
22	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.0306961690840033	2.237485424380664E-4
23	6.392815498230469E-5	3.1964077491152344E-5	0.0	0.04419566447776664	2.876766974203711E-4
24	7.458284747935546E-5	3.1964077491152344E-5	0.0	0.06588861840176204	2.9833138991742185E-4
25	7.458284747935546E-5	3.1964077491152344E-5	0.0	0.0803790001977511	2.9833138991742185E-4
26	7.458284747935546E-5	3.1964077491152344E-5	0.0	0.10571585895573785	3.089860824144727E-4
27	7.458284747935546E-5	3.1964077491152344E-5	0.0	0.16341101882726783	3.3029546740857423E-4
28	7.458284747935546E-5	3.1964077491152344E-5	0.0	0.2657706496464347	3.3029546740857423E-4
29	7.458284747935546E-5	4.2618769988203125E-5	0.0	0.3586156400657352	3.40950159905625E-4
30	7.458284747935546E-5	4.2618769988203125E-5	0.0	0.5036473143355904	3.40950159905625E-4
31	8.523753997640625E-5	4.2618769988203125E-5	0.0	0.8414010664921002	3.40950159905625E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	3065	0.0	17.081566	5
CAAGACG	3155	0.0	16.828842	4
TCTATAC	645	0.0	15.2015505	3
TAATACG	165	1.6496415E-8	14.575759	4
AAAAAGT	3650	0.0	14.090412	1
TATAACG	355	0.0	14.070422	2
TACCGTC	2540	0.0	13.984252	7
CGCAAGA	3725	0.0	13.955705	2
AGTACTC	3930	0.0	13.933843	5
AAAAGTA	3745	0.0	13.881174	2
ACCGTCG	2535	0.0	13.865878	8
AAGTACT	4140	0.0	13.718599	4
CGAACGT	1360	0.0	13.6029415	4
GACGGAC	3785	0.0	13.587847	7
ATACCGT	2630	0.0	13.576045	6
CTTATAC	8675	0.0	13.541786	37
TGGGCTG	6700	0.0	13.419403	36
GCGCAAG	3825	0.0	13.397387	1
CGGTCCA	2200	0.0	13.286365	10
TATCACG	350	0.0	13.214285	2
>>END_MODULE