FastQCFastQC Report
Thu 9 Feb 2017
SRR2727169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6108090
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG366650.6002694786750031No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC360510.5902172364847277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT297610.4872390550892341No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA233480.3822471509096952No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG219380.35916301167795495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193170.31625270747484074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189300.3099168479835759No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT156060.25549721762449473No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG154650.2531888037013207No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144080.23588388514249134No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG135180.22131304548557731No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC130840.2142077146865878No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC125150.20489220034413377No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT116900.191385523134073No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT116620.19092711469542853No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG109410.179123097400333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT106140.17376954170616346No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC103630.16966023748831469No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA85990.14078050585371205No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT83600.13686766239528234No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT81990.13423181387307653No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC78280.12815790206103708No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC78270.1281415303310855No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT75220.12314815269585092No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC73970.12110168645190231No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT73170.1197919480557752No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72830.11923530923742119No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC69900.11443839236160568No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT68910.11281759109639838No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA67410.11036183160366006No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG64670.10587597759692473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG19550.015.3299235
CAAGACG20800.014.9423074
TACCGTC16600.014.3765067
CTTATAC36200.014.25828737
ATACCGT16600.014.1536156
TCTTATA57550.013.75847137
GTTGCGC3100.013.7258078
ACCGTCG17500.013.6371438
CCGTCGT16600.013.5963869
GTATAGA5350.013.4859811
TCTAGCG14100.013.382977528
CGTCGTA17150.013.37609310
CTAGCGG14050.013.29893229
TACTTAC8100.013.01851831
CGAACGT7600.012.9013164
ACGGACC23400.012.8867528
AGTACTC15400.012.8538975
AGACGGA23000.012.7891316
GCGCAAG23850.012.7211731
GACGGAC23150.012.7062647
Produced by FastQC (version 0.11.5)