##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6108090
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170571979129317	31.0	31.0	33.0	30.0	34.0
2	31.308672269072655	31.0	31.0	34.0	30.0	34.0
3	31.358915471121087	31.0	31.0	34.0	28.0	34.0
4	35.19244722982143	35.0	35.0	37.0	33.0	37.0
5	35.03645820542919	35.0	35.0	37.0	32.0	37.0
6	35.07034539438679	36.0	35.0	37.0	32.0	37.0
7	34.880934465602174	36.0	35.0	37.0	32.0	37.0
8	34.807096490064815	36.0	35.0	37.0	32.0	37.0
9	36.42862073086677	38.0	35.0	39.0	32.0	39.0
10	36.21401452827316	38.0	35.0	39.0	32.0	39.0
11	36.33424458382244	38.0	35.0	39.0	32.0	39.0
12	36.19992812810551	38.0	35.0	39.0	32.0	39.0
13	36.30207822740005	38.0	35.0	39.0	32.0	39.0
14	37.26947785641666	39.0	36.0	41.0	32.0	41.0
15	37.15403816905121	39.0	36.0	41.0	31.0	41.0
16	37.10346294831936	39.0	36.0	40.0	31.0	41.0
17	37.01773091097217	39.0	36.0	40.0	31.0	41.0
18	37.01746618009885	39.0	36.0	40.0	31.0	41.0
19	37.03676533908308	39.0	36.0	40.0	31.0	41.0
20	36.91892260919535	39.0	36.0	40.0	31.0	41.0
21	36.78522353141489	39.0	36.0	40.0	30.0	41.0
22	36.7425776306505	39.0	35.0	40.0	30.0	41.0
23	36.52805901681213	38.0	35.0	40.0	30.0	41.0
24	36.4123763729742	38.0	35.0	40.0	30.0	41.0
25	36.287950406755634	38.0	35.0	40.0	30.0	41.0
26	35.959740933745245	38.0	34.0	40.0	28.0	41.0
27	36.10094022845112	38.0	35.0	40.0	29.0	41.0
28	35.969162209463185	38.0	34.0	40.0	28.0	41.0
29	35.86685625129951	38.0	34.0	40.0	27.0	41.0
30	35.99548320342365	38.0	35.0	40.0	29.0	41.0
31	35.944655203181355	38.0	34.0	40.0	29.0	41.0
32	35.76274465503946	38.0	34.0	40.0	27.0	41.0
33	35.685623001625714	38.0	34.0	40.0	27.0	41.0
34	35.7050388910445	38.0	34.0	40.0	27.0	41.0
35	35.42078833154063	38.0	34.0	40.0	26.0	41.0
36	35.28525823948239	38.0	34.0	40.0	26.0	41.0
37	35.077302397312415	38.0	33.0	40.0	25.0	41.0
38	34.935101316450805	38.0	33.0	40.0	25.0	41.0
39	34.868066777012125	38.0	33.0	40.0	24.0	41.0
40	34.73501438256476	38.0	33.0	40.0	24.0	41.0
41	34.563864808802755	38.0	33.0	40.0	24.0	41.0
42	34.39235554813371	38.0	33.0	40.0	23.0	41.0
43	33.96131622160119	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	2.0
13	12.0
14	23.0
15	79.0
16	254.0
17	467.0
18	962.0
19	1896.0
20	3858.0
21	7154.0
22	12716.0
23	20868.0
24	32856.0
25	48488.0
26	70116.0
27	96065.0
28	128996.0
29	166820.0
30	209475.0
31	255487.0
32	302657.0
33	359732.0
34	431506.0
35	513708.0
36	619873.0
37	777918.0
38	1017527.0
39	1028570.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.0124457891092	19.668210520801104	12.433968720172754	25.885374969916946
2	17.65501163211413	23.872274311609683	32.831638040696845	25.64107601557934
3	21.102210347260765	25.053347282047252	29.587759839818993	24.25668253087299
4	15.496186205507778	16.755974453552582	33.15630581736681	34.591533523572835
5	14.240081596702078	37.57878485745954	33.37958347044657	14.801550075391818
6	34.16745660263683	33.5422038640557	16.24019947315773	16.050140060149737
7	26.90988508682747	31.33007208472698	22.317287400807782	19.442755427637774
8	27.85595824553993	31.927967662559	21.70488647023865	18.511187621662415
9	27.878371143843655	13.587177006232718	19.946579045167965	38.587872804755655
10	17.854337444274723	26.599902751924088	32.61615333107403	22.929606472727155
11	35.19348929043285	22.277536840485325	20.4301999479379	22.098773921143923
12	21.649157101483443	26.747968677606256	28.877488707599262	22.725385513311036
13	30.790410750332754	19.00657979826754	25.021029487122814	25.181979964276884
14	23.05763340094858	21.853394432629514	25.045881773189326	30.04309039323258
15	27.329132347427752	26.082572457183833	22.57396338298879	24.014331812399618
16	24.00630966472334	25.476360040536406	26.659823283546903	23.857507011193352
17	24.561573257761427	26.699459241759698	25.79295655434023	22.946010946138646
18	24.9044300264076	23.01375716467832	28.232098741177687	23.849714067736397
19	24.35136024518303	27.0678067939405	26.86636902861615	21.71446393226033
20	24.095339132200085	24.223333316961604	27.319276565996898	24.362050984841417
21	23.945013907784592	26.252609244461034	26.726816402508803	23.075560445245568
22	24.38786920297507	25.82034645854924	24.84485330111377	24.946931037361924
23	22.279747024028786	25.103133712830033	26.67608041138883	25.941038851752346
24	23.03271562796226	27.94393992229977	25.73434576111354	23.28899868862443
25	24.518188173389717	25.05187382635161	24.378651919012327	26.05128608124635
26	23.754479714608003	25.93877955301903	26.36572152669656	23.941019205676405
27	25.253344335135864	25.287151957485893	24.900844617548202	24.558659089830044
28	23.233302063329127	25.873292633212674	26.954432564025744	23.938972739432458
29	23.861370739461925	25.172582591284677	27.001353942067	23.964692727186403
30	23.11082515156129	26.990646830678656	27.35604747146817	22.542480546291884
31	25.14864712209545	25.554273103376012	24.40021348735857	24.896866287169967
32	21.816345207749066	26.503620608078794	26.248876490032075	25.431157694140065
33	22.988462841903115	25.683151361554923	26.909508537038583	24.41887725950338
34	23.24271580805129	25.732266551409687	26.759281543002807	24.26573609753622
35	23.322429761185575	26.6237072472737	27.52729576676179	22.52656722477894
36	23.863122514566747	26.084684410347585	26.025091313323806	24.02710176176186
37	23.15255669120789	25.9771385162956	27.056886850062785	23.813417942433723
38	23.4221172248608	25.20781455414049	27.469372586193064	23.900695634805643
39	23.698095476654732	23.52118256279786	27.216151039031843	25.56457092151556
40	23.46766337758612	24.898470716705223	27.89474287379525	23.739123031913415
41	22.127850113537946	24.691793997796367	28.349533160120433	24.830822728545257
42	23.621033743772603	25.20213356384729	27.99028828979272	23.18654440258739
43	22.535195126463428	23.345481156957412	28.647384043129687	25.471939673449473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	992.0
1	1166.0
2	1340.0
3	3790.5
4	6241.0
5	6241.0
6	9421.0
7	12601.0
8	12693.0
9	12785.0
10	16522.0
11	20259.0
12	20259.0
13	32844.5
14	45430.0
15	60721.5
16	76013.0
17	67979.0
18	59945.0
19	59945.0
20	62279.5
21	64614.0
22	56380.0
23	48146.0
24	53537.5
25	58929.0
26	58929.0
27	66422.5
28	73916.0
29	80367.0
30	86818.0
31	97015.5
32	107213.0
33	107213.0
34	126730.5
35	146248.0
36	175041.0
37	203834.0
38	231812.0
39	259790.0
40	259790.0
41	281971.0
42	304152.0
43	325161.0
44	346170.0
45	394489.0
46	442808.0
47	442808.0
48	601804.5
49	760801.0
50	755309.0
51	749817.0
52	596801.5
53	443786.0
54	443786.0
55	416042.5
56	388299.0
57	373752.5
58	359206.0
59	335419.0
60	311632.0
61	311632.0
62	263081.5
63	214531.0
64	189202.5
65	163874.0
66	139756.0
67	115638.0
68	115638.0
69	98482.0
70	81326.0
71	69984.0
72	58642.0
73	44447.5
74	30253.0
75	30253.0
76	24039.5
77	17826.0
78	15332.5
79	12839.0
80	10623.5
81	8408.0
82	8408.0
83	7063.5
84	5719.0
85	5018.0
86	4317.0
87	2946.0
88	1575.0
89	1575.0
90	1152.5
91	730.0
92	536.0
93	342.0
94	302.0
95	262.0
96	262.0
97	136.0
98	10.0
99	11.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6108090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.34666392906507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16102936306007	33.97084469140492
2	9.101164166916018	7.526055523454522
3	2.964239353032474	3.67684225005429
4	1.5051537411252205	2.48932343583518
5	0.9224650975023961	1.9070427186361913
6	0.5754621274670423	1.4276063512970762
7	0.4169162569844759	1.2066667454870597
8	0.31791689007206403	1.0515842248946525
9	0.24887772365093092	0.9261237239303196
>10	1.542247955798205	11.882786300796752
>50	0.12073102447159428	3.4538590604121504
>100	0.09462074409327666	8.106402981682466
>500	0.01506869665887357	4.3822432561359514
>1k	0.012583959598165068	10.327469298261176
>5k	8.015260890548287E-4	2.265314081339154
>10k+	7.213734801493459E-4	5.399835356378305
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	36665	0.6002694786750031	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	36051	0.5902172364847277	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29761	0.4872390550892341	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	23348	0.3822471509096952	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	21938	0.35916301167795495	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19317	0.31625270747484074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18930	0.3099168479835759	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15606	0.25549721762449473	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	15465	0.2531888037013207	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14408	0.23588388514249134	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	13518	0.22131304548557731	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	13084	0.2142077146865878	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	12515	0.20489220034413377	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	11690	0.191385523134073	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	11662	0.19092711469542853	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	10941	0.179123097400333	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	10614	0.17376954170616346	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	10363	0.16966023748831469	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	8599	0.14078050585371205	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	8360	0.13686766239528234	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	8199	0.13423181387307653	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	7828	0.12815790206103708	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	7827	0.1281415303310855	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	7522	0.12314815269585092	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	7397	0.12110168645190231	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	7317	0.1197919480557752	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7283	0.11923530923742119	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	6990	0.11443839236160568	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	6891	0.11281759109639838	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	6741	0.11036183160366006	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	6467	0.10587597759692473	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.911518985476638E-5
2	0.0	0.0	0.0	0.0	4.911518985476638E-5
3	0.0	0.0	0.0	1.6371729951588794E-5	4.911518985476638E-5
4	0.0	0.0	0.0	6.548691980635518E-5	4.911518985476638E-5
5	0.0	0.0	0.0	6.548691980635518E-5	4.911518985476638E-5
6	0.0	0.0	0.0	6.548691980635518E-5	8.185864975794397E-5
7	0.0	0.0	0.0	8.185864975794397E-5	8.185864975794397E-5
8	0.0	0.0	0.0	9.823037970953276E-5	9.823037970953276E-5
9	0.0	0.0	0.0	1.1460210966112156E-4	1.6371729951588794E-4
10	0.0	0.0	0.0	1.1460210966112156E-4	1.6371729951588794E-4
11	0.0	0.0	0.0	1.1460210966112156E-4	1.6371729951588794E-4
12	0.0	0.0	0.0	1.6371729951588794E-4	1.8008902946747675E-4
13	0.0	0.0	0.0	2.1283248937065434E-4	1.9646075941906553E-4
14	1.6371729951588794E-5	0.0	0.0	2.783194091770095E-4	2.946911391285983E-4
15	3.274345990317759E-5	0.0	0.0	4.5840843864448623E-4	3.1106286908018707E-4
16	3.274345990317759E-5	0.0	0.0	0.001031418986950094	3.274345990317759E-4
17	3.274345990317759E-5	0.0	0.0	0.0018008902946747673	3.274345990317759E-4
18	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.0024230160328351418	3.601780589349535E-4
19	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.003732754428962245	3.601780589349535E-4
20	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.005009749365186171	3.601780589349535E-4
21	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.008038519406230097	3.601780589349535E-4
22	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.012688090712481316	3.601780589349535E-4
23	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.01859828522500487	4.0929324878971986E-4
24	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.028585040495474036	4.2566497874130867E-4
25	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.03546116707514133	4.2566497874130867E-4
26	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.04787093837844564	4.420367086928975E-4
27	3.274345990317759E-5	1.6371729951588794E-5	0.0	0.08698300123279126	4.420367086928975E-4
28	3.274345990317759E-5	3.274345990317759E-5	0.0	0.14551193580972122	4.420367086928975E-4
29	3.274345990317759E-5	3.274345990317759E-5	0.0	0.20284573410018517	4.420367086928975E-4
30	3.274345990317759E-5	3.274345990317759E-5	0.0	0.29582078849525795	4.420367086928975E-4
31	3.274345990317759E-5	3.274345990317759E-5	0.0	0.5362068993744362	4.5840843864448623E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1955	0.0	15.329923	5
CAAGACG	2080	0.0	14.942307	4
TACCGTC	1660	0.0	14.376506	7
CTTATAC	3620	0.0	14.258287	37
ATACCGT	1660	0.0	14.153615	6
TCTTATA	5755	0.0	13.758471	37
GTTGCGC	310	0.0	13.725807	8
ACCGTCG	1750	0.0	13.637143	8
CCGTCGT	1660	0.0	13.596386	9
GTATAGA	535	0.0	13.485981	1
TCTAGCG	1410	0.0	13.3829775	28
CGTCGTA	1715	0.0	13.376093	10
CTAGCGG	1405	0.0	13.298932	29
TACTTAC	810	0.0	13.018518	31
CGAACGT	760	0.0	12.901316	4
ACGGACC	2340	0.0	12.886752	8
AGTACTC	1540	0.0	12.853897	5
AGACGGA	2300	0.0	12.789131	6
GCGCAAG	2385	0.0	12.721173	1
GACGGAC	2315	0.0	12.706264	7
>>END_MODULE