FastQCFastQC Report
Thu 9 Feb 2017
SRR2727168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671488
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78840.2951164294954722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54940.20565317905227348No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT43250.1618947942120646No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40680.15227468736524363No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30520.11424344784629391No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30510.11420601552393274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30040.1124466963729577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.11098683580087203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC2250.020.5555553
CGCCGTA851.2465716E-619.58823633
AGGTTAT4950.019.4343431
AAGACGG9350.019.3903735
CGAACGT2150.018.9302334
GGTTATC5150.018.6796112
ACGAACG4400.018.515
CAAGACG9950.018.4070364
TATACTA3450.018.2318845
TAACGAA4800.017.3437513
CGAACGA4700.016.92553316
CCGATAA5050.016.8514869
CGCAAGA10900.016.4633032
ATTCCGA5400.016.4444436
AATTAAC4400.016.3977282
TTATCTA5650.016.3716814
TCGAACG2500.016.2799993
CGATAAC5300.016.05660410
CGAGACT5200.016.00961520
TTAGAGT5450.015.9541284
Produced by FastQC (version 0.11.5)