##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671488
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.986012664103303	31.0	31.0	33.0	30.0	34.0
2	31.23442703092808	31.0	31.0	34.0	30.0	34.0
3	31.363728379090603	31.0	31.0	34.0	30.0	34.0
4	35.116926971036364	35.0	35.0	37.0	32.0	37.0
5	34.868316458842415	35.0	35.0	37.0	32.0	37.0
6	35.01436689964544	35.0	35.0	37.0	32.0	37.0
7	34.934013179172055	35.0	35.0	37.0	32.0	37.0
8	34.91088786474055	35.0	35.0	37.0	32.0	37.0
9	36.37802827487902	38.0	35.0	39.0	32.0	39.0
10	36.06300907958411	38.0	35.0	39.0	31.0	39.0
11	36.21122273429639	38.0	35.0	39.0	32.0	39.0
12	35.9970020453021	37.0	35.0	39.0	30.0	39.0
13	36.03692511439318	37.0	35.0	39.0	31.0	39.0
14	36.88654450253941	38.0	36.0	40.0	31.0	41.0
15	36.83425678872598	38.0	36.0	40.0	31.0	41.0
16	36.72005451643429	38.0	36.0	40.0	31.0	41.0
17	37.01403599791577	39.0	36.0	40.0	31.0	41.0
18	37.02980997855877	39.0	36.0	40.0	31.0	41.0
19	37.085609592856116	39.0	36.0	40.0	31.0	41.0
20	37.04605822672608	39.0	36.0	40.0	31.0	41.0
21	36.978562134660535	39.0	36.0	40.0	31.0	41.0
22	36.918275133558524	39.0	36.0	40.0	31.0	41.0
23	36.752120166738536	39.0	36.0	40.0	30.0	41.0
24	36.61431344628911	39.0	36.0	40.0	30.0	41.0
25	36.53678848641656	39.0	35.0	40.0	30.0	41.0
26	36.194112045421875	38.0	35.0	40.0	29.0	41.0
27	35.968826361937616	38.0	35.0	40.0	27.0	41.0
28	35.72216382779934	38.0	34.0	40.0	27.0	41.0
29	35.51907738309137	38.0	34.0	40.0	27.0	41.0
30	35.359419918786834	38.0	34.0	40.0	26.0	41.0
31	35.17549695151167	38.0	34.0	40.0	25.0	41.0
32	34.983713196540656	38.0	34.0	40.0	25.0	41.0
33	34.80100715406545	38.0	33.0	40.0	25.0	41.0
34	34.66134191881079	38.0	33.0	40.0	24.0	41.0
35	34.47331187712616	38.0	33.0	40.0	24.0	41.0
36	34.3493573618897	38.0	33.0	40.0	24.0	41.0
37	34.248719440252025	38.0	33.0	40.0	23.0	41.0
38	34.126439272794784	38.0	33.0	40.0	23.0	41.0
39	33.94906134708447	38.0	32.0	40.0	22.0	41.0
40	33.83674491519333	37.0	32.0	40.0	21.0	41.0
41	33.57683059029275	37.0	32.0	40.0	19.0	41.0
42	33.36228648603325	37.0	31.0	40.0	18.0	41.0
43	33.07336772615112	37.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	18.0
11	31.0
12	29.0
13	58.0
14	116.0
15	204.0
16	308.0
17	532.0
18	999.0
19	1779.0
20	3420.0
21	5736.0
22	9395.0
23	14338.0
24	20258.0
25	27802.0
26	36577.0
27	47532.0
28	59703.0
29	73281.0
30	89939.0
31	107568.0
32	129423.0
33	155997.0
34	188918.0
35	231708.0
36	294270.0
37	391480.0
38	543840.0
39	236225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.05141104882373	24.468049266925398	14.916705596281924	23.563834087968953
2	21.687276903358725	25.548682981170046	30.632216951751236	22.131823163719993
3	21.68929824876623	26.284302980211777	29.43015278376695	22.596245987255042
4	17.178366513343875	18.533641176752433	32.97372101240956	31.314271297494127
5	13.367194612141247	40.24554854822481	33.496687988117486	12.89056885151646
6	33.823846485554114	38.94574858655551	14.137439509367065	13.092965418523308
7	28.235425350965453	32.464828589909445	21.09412432322361	18.205621735901488
8	27.223143057352306	36.324213322313256	18.896248083465096	17.55639553686934
9	27.987286486033252	13.667514134444925	17.78746526280485	40.55773411671698
10	16.12846473575775	28.39937892290738	33.322178501269704	22.149977840065162
11	37.84385331320972	22.311123987830005	20.077350150927124	19.767672548033154
12	21.10879779358919	26.650465957548754	29.35098342197307	22.889752826888984
13	30.46455757989555	20.246319654065452	24.49144446840113	24.79767829763787
14	22.516103385079774	21.109134684490442	24.54617052369316	31.828591406736624
15	25.440428704901535	28.935409779119354	21.211549518470605	24.412611997508506
16	24.86344688802645	27.017152987398784	23.812497005414208	24.30690311916056
17	24.195654257103158	27.48374688563078	24.299790977911936	24.020807879354127
18	24.73947103636625	25.520945630300417	25.774811640553878	23.964771692779454
19	25.320158653155094	26.211160222318046	25.200861841790044	23.267819282736813
20	24.626575152124957	25.06019117435676	25.341944264769296	24.97128940874898
21	25.1419059340712	26.089018554453546	25.21557274447798	23.55350276699727
22	25.09631336543529	25.608799290882082	24.64618968904221	24.64869765464041
23	23.957322660629583	25.4009376048105	25.64989998083465	24.991839753725266
24	24.2827592712376	26.055554058262658	25.19086741171961	24.470819258780125
25	24.88744100665996	25.255587897082076	24.833575894782236	25.023395201475733
26	24.776753629437977	25.858023693162764	25.098596737099328	24.26662594029994
27	25.48579667960328	25.719449235781706	24.439263811029658	24.35549027358536
28	24.607110344497148	26.061618094485173	25.14134444923578	24.1899271117819
29	24.85940419721144	25.944791816395956	25.37754240333477	23.818261583057833
30	25.09627593311293	25.99472653442576	24.94497448612908	23.96402304633223
31	25.56350618082507	25.473556310191174	24.888975731876766	24.07396177710699
32	24.399585549326815	25.951716796032777	24.78229361314743	24.86640404149298
33	24.544897824732885	25.335842796224426	25.127045302093816	24.992214076948876
34	24.953097300081453	25.626916534904893	25.57200331800105	23.8479828470126
35	25.248475755833454	25.700733074601125	25.352088424129178	23.69870274543625
36	24.7686308154856	25.814302740644912	25.188771201667386	24.2282952422021
37	24.83469886445307	25.778816899046525	25.42234889320109	23.964135343299315
38	25.038068671841312	24.756090987494613	25.738577152508263	24.467263188155812
39	25.024780197403096	25.07254384073595	25.309864764505775	24.592811197355182
40	25.16024777202817	25.126933005126727	25.758453715682045	23.95436550716305
41	24.111805855014133	25.514844161755544	25.87834944420488	24.49500053902544
42	25.142055663360647	25.521844006037085	25.750068875473143	23.586031455129127
43	24.51603750419242	24.889013164199127	25.7857793110057	24.80917002060275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	404.0
1	395.0
2	386.0
3	399.5
4	413.0
5	413.0
6	614.0
7	815.0
8	1047.0
9	1279.0
10	1801.0
11	2323.0
12	2323.0
13	5079.5
14	7836.0
15	11875.5
16	15915.0
17	13607.0
18	11299.0
19	11299.0
20	14623.0
21	17947.0
22	17683.5
23	17420.0
24	21155.5
25	24891.0
26	24891.0
27	30431.5
28	35972.0
29	41499.5
30	47027.0
31	54247.0
32	61467.0
33	61467.0
34	72573.0
35	83679.0
36	97801.0
37	111923.0
38	124843.0
39	137763.0
40	137763.0
41	148182.5
42	158602.0
43	168128.5
44	177655.0
45	193835.0
46	210015.0
47	210015.0
48	231370.5
49	252726.0
50	251857.0
51	250988.0
52	234837.5
53	218687.0
54	218687.0
55	206663.0
56	194639.0
57	184696.5
58	174754.0
59	159926.5
60	145099.0
61	145099.0
62	122685.0
63	100271.0
64	86263.0
65	72255.0
66	60827.0
67	49399.0
68	49399.0
69	40094.5
70	30790.0
71	26843.5
72	22897.0
73	18075.5
74	13254.0
75	13254.0
76	10231.0
77	7208.0
78	6224.0
79	5240.0
80	4018.5
81	2797.0
82	2797.0
83	2331.0
84	1865.0
85	1523.5
86	1182.0
87	1180.0
88	1178.0
89	1178.0
90	856.0
91	534.0
92	423.5
93	313.0
94	289.0
95	265.0
96	265.0
97	161.5
98	58.0
99	58.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2671488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.4256193059177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98900816343757	48.2312080867518
2	8.436587268489363	9.689524974428446
3	2.821896018851229	4.8614737949830635
4	1.3980960588449385	3.2114612811333343
5	0.7877594310957023	2.2618786597374054
6	0.5141243142026491	1.7714344285990409
7	0.3618038327665221	1.4543766414710546
8	0.2622076098110507	1.2045947504099204
9	0.2053446203939642	1.0612837797535775
>10	1.0901036675272817	11.188379099824953
>50	0.0723188123265581	2.8814648920930717
>100	0.050357050621512196	5.9730196958525275
>500	0.007794863723674859	3.054778669217754
>1k	0.0024683735124880805	2.65609411233008
>5k	1.299143953941095E-4	0.49902713341411153
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7884	0.2951164294954722	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5494	0.20565317905227348	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4325	0.1618947942120646	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4068	0.15227468736524363	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3052	0.11424344784629391	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3051	0.11420601552393274	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.1124466963729577	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2965	0.11098683580087203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7432322361171004E-5
2	0.0	0.0	0.0	0.0	3.7432322361171004E-5
3	0.0	0.0	0.0	0.0	3.7432322361171004E-5
4	0.0	0.0	0.0	0.0	3.7432322361171004E-5
5	0.0	0.0	0.0	0.0	3.7432322361171004E-5
6	0.0	0.0	0.0	0.0	7.486464472234201E-5
7	0.0	0.0	0.0	0.0	7.486464472234201E-5
8	0.0	0.0	0.0	0.0	1.1229696708351301E-4
9	0.0	0.0	0.0	3.7432322361171004E-5	2.9945857888936803E-4
10	0.0	0.0	0.0	3.7432322361171004E-5	2.9945857888936803E-4
11	0.0	0.0	0.0	3.7432322361171004E-5	3.3689090125053905E-4
12	0.0	0.0	0.0	3.7432322361171004E-5	3.3689090125053905E-4
13	0.0	0.0	0.0	3.7432322361171004E-5	3.3689090125053905E-4
14	0.0	0.0	0.0	7.486464472234201E-5	3.7432322361171E-4
15	0.0	0.0	0.0	7.486464472234201E-5	3.7432322361171E-4
16	0.0	0.0	0.0	1.4972928944468402E-4	4.4918786833405205E-4
17	0.0	0.0	0.0	1.4972928944468402E-4	4.4918786833405205E-4
18	0.0	0.0	0.0	1.87161611805855E-4	4.4918786833405205E-4
19	0.0	0.0	0.0	2.62026256528197E-4	4.4918786833405205E-4
20	0.0	0.0	0.0	2.9945857888936803E-4	4.4918786833405205E-4
21	0.0	0.0	0.0	5.24052513056394E-4	4.86620190695223E-4
22	0.0	0.0	0.0	7.112141248622491E-4	4.86620190695223E-4
23	0.0	0.0	0.0	9.358080590292751E-4	6.737818025010781E-4
24	0.0	0.0	0.0	0.001684454506252695	6.737818025010781E-4
25	0.0	0.0	0.0	0.0018716161180585501	6.737818025010781E-4
26	0.0	0.0	0.0	0.0026951272100043124	7.112141248622491E-4
27	0.0	0.0	0.0	0.006662953380288439	7.112141248622491E-4
28	0.0	0.0	0.0	0.013251042115854535	7.112141248622491E-4
29	0.0	0.0	0.0	0.023432633798093048	7.112141248622491E-4
30	0.0	0.0	0.0	0.04076379905131522	7.112141248622491E-4
31	0.0	0.0	0.0	0.10750562982128312	7.112141248622491E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	225	0.0	20.555555	3
CGCCGTA	85	1.2465716E-6	19.588236	33
AGGTTAT	495	0.0	19.434343	1
AAGACGG	935	0.0	19.390373	5
CGAACGT	215	0.0	18.930233	4
GGTTATC	515	0.0	18.679611	2
ACGAACG	440	0.0	18.5	15
CAAGACG	995	0.0	18.407036	4
TATACTA	345	0.0	18.231884	5
TAACGAA	480	0.0	17.34375	13
CGAACGA	470	0.0	16.925533	16
CCGATAA	505	0.0	16.851486	9
CGCAAGA	1090	0.0	16.463303	2
ATTCCGA	540	0.0	16.444443	6
AATTAAC	440	0.0	16.397728	2
TTATCTA	565	0.0	16.371681	4
TCGAACG	250	0.0	16.279999	3
CGATAAC	530	0.0	16.056604	10
CGAGACT	520	0.0	16.009615	20
TTAGAGT	545	0.0	15.954128	4
>>END_MODULE