FastQCFastQC Report
Thu 9 Feb 2017
SRR2727167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6144206
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT268440.43689941385428804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174510.28402368019561847No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG170450.27741582883125987No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC166590.27113348738632786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163910.26677165446601236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116310.1893002936424983No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA104890.1707136772432435No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG94340.15354302899349404No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC81240.13222212927105634No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT77830.12667218514483403No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG73870.12022708874018873No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG69770.11355413539194488No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC67200.10937133292731396No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT67000.10904582300788743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG20000.017.024
AAGACGG19900.016.9195985
CTTATAC44950.016.05116837
TCTAGCG13700.014.313868528
TCTTATA71450.014.24072637
CGCAAGA23400.014.0726492
ACGGACC23700.014.0506328
CTAGCGG14000.014.00714229
GCGCAAG23850.013.9622631
GACGGAC23600.013.8757
GCAAGAC29150.013.3910813
AGACGGA25400.013.328746
CGGACCA24650.013.2839779
TATACTG5750.013.1913035
CGCAATA15950.012.99059536
TAGCGGC15250.012.98032830
CGGTCCA16150.012.94427310
ACCGTCG16350.012.8990828
TCTAGAT13500.012.8814822
TTAATAC6350.012.8188973
Produced by FastQC (version 0.11.5)