##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727167.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6144206 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.134032940952824 31.0 31.0 33.0 30.0 34.0 2 31.291999649751325 31.0 31.0 34.0 30.0 34.0 3 31.34647829190623 31.0 31.0 34.0 28.0 34.0 4 35.19748279924208 35.0 35.0 37.0 33.0 37.0 5 35.04292955021365 35.0 35.0 37.0 32.0 37.0 6 35.05326481566536 36.0 35.0 37.0 32.0 37.0 7 34.874150215666596 36.0 35.0 37.0 32.0 37.0 8 34.78861695066865 36.0 35.0 37.0 32.0 37.0 9 36.41665676573995 38.0 35.0 39.0 32.0 39.0 10 36.20865901957063 38.0 35.0 39.0 32.0 39.0 11 36.30736241590858 38.0 35.0 39.0 32.0 39.0 12 36.16781533692067 38.0 35.0 39.0 31.0 39.0 13 36.272878708819334 38.0 35.0 39.0 32.0 39.0 14 37.24847929903392 39.0 36.0 40.0 31.0 41.0 15 37.1412802565539 39.0 36.0 40.0 31.0 41.0 16 37.12668829788585 39.0 36.0 40.0 31.0 41.0 17 37.0061443252391 39.0 36.0 40.0 31.0 41.0 18 37.01916504752607 39.0 36.0 40.0 31.0 41.0 19 37.04520486455044 39.0 36.0 40.0 31.0 41.0 20 36.90945388224288 39.0 36.0 40.0 31.0 41.0 21 36.78937848112515 39.0 36.0 40.0 30.0 41.0 22 36.72409811780399 39.0 35.0 40.0 30.0 41.0 23 36.51542314824731 38.0 35.0 40.0 30.0 41.0 24 36.39524032885616 38.0 35.0 40.0 30.0 41.0 25 36.27462555780193 38.0 35.0 40.0 29.0 41.0 26 35.978211342523345 38.0 34.0 40.0 28.0 41.0 27 36.13082943508079 38.0 35.0 40.0 29.0 41.0 28 36.01576721223214 38.0 34.0 40.0 29.0 41.0 29 35.92355090307844 38.0 34.0 40.0 28.0 41.0 30 36.07381344310396 38.0 35.0 40.0 29.0 41.0 31 36.03508411013563 38.0 35.0 40.0 29.0 41.0 32 35.89910396884479 38.0 34.0 40.0 28.0 41.0 33 35.81098387651716 38.0 34.0 40.0 27.0 41.0 34 35.851363219267064 38.0 34.0 40.0 28.0 41.0 35 35.56728338861034 38.0 34.0 40.0 27.0 41.0 36 35.429986396940464 38.0 34.0 40.0 26.0 41.0 37 35.24752278813568 38.0 34.0 40.0 25.0 41.0 38 35.107577122251435 38.0 33.0 40.0 25.0 41.0 39 35.06202379933225 38.0 33.0 40.0 25.0 41.0 40 34.95069664005406 38.0 33.0 40.0 25.0 41.0 41 34.790642761652194 38.0 33.0 40.0 24.0 41.0 42 34.641663056219144 38.0 33.0 40.0 24.0 41.0 43 34.234003059142225 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 9.0 13 16.0 14 28.0 15 85.0 16 244.0 17 449.0 18 887.0 19 1651.0 20 3409.0 21 6597.0 22 11659.0 23 19485.0 24 30984.0 25 46625.0 26 67325.0 27 93787.0 28 127120.0 29 165933.0 30 208290.0 31 253478.0 32 304360.0 33 361588.0 34 432554.0 35 515749.0 36 624896.0 37 776564.0 38 1026884.0 39 1063546.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.235808174400404 19.929214612921506 12.992793535893815 25.842183676784273 2 18.0606900224374 22.91287108537702 34.15197993036041 24.874458961825173 3 20.29956352374904 24.548070165616192 29.678936546072837 25.473429764561928 4 15.064989682963104 16.48173905627513 34.050192978555735 34.40307828220603 5 14.505193999029329 37.15441832516683 33.39964512908584 14.940742546717997 6 34.24126730125911 34.69672078052071 15.872628619548237 15.189383298671952 7 27.922485020847283 31.015301244782485 22.03851889080542 19.023694843564815 8 27.087421873550465 33.24842949601624 20.901675497208263 18.762473133225026 9 27.34171022260647 13.984215372987169 19.773148882052457 38.90092552235391 10 17.412290538435723 26.38640371107349 32.743644988465554 23.45766076202523 11 35.87332521077581 21.961275386925504 21.029145181655693 21.136254220642993 12 21.69048368495457 26.07941530606233 29.19701911036186 23.033081898621237 13 30.255186756433623 19.132643013596876 25.254491792755644 25.357678437213853 14 22.89041090093659 21.249857182522852 25.01512807350535 30.844603843035213 15 25.930380589452895 27.034135899740342 22.517230053810046 24.51825345699672 16 24.3534315092951 25.72973627511838 25.506355092911924 24.4104771226746 17 24.094276786943666 26.574580995493967 25.70818751845234 23.622954699110025 18 24.63245861222752 23.852699600241266 27.4369381495347 24.077903637996513 19 24.382971534483055 26.299687868538264 26.550151476041005 22.76718912093768 20 24.293407480152844 24.569163208395032 26.7563131835098 24.381116127942327 21 24.743294739792255 25.89686934324793 25.943303333254125 23.416532583705692 22 24.634216365792422 25.44862590870163 25.230452885205995 24.68670484029995 23 22.977435977895272 25.089718671541938 26.210026161232225 25.722819189330565 24 23.608209099760003 26.967634223201504 25.67856611578453 23.74559056125397 25 24.57419233665017 25.068462873803384 24.978768615505402 25.37857617404104 26 23.90071231335668 25.86939630604833 26.18870526150979 24.0411861190852 27 25.094959381244703 25.305482921633814 25.143932348622428 24.45562534849906 28 23.54204920863656 25.71824577496262 26.651645468918193 24.088059547482622 29 23.813361726478572 25.576811714971797 26.714647913823203 23.895178644726432 30 23.576455607119946 26.444458405203207 26.83230347419992 23.146782513476925 31 24.66071287323374 25.607979289756887 25.018480825675443 24.71282701133393 32 22.778581968117606 26.01668954458884 25.89307715268661 25.311651334606942 33 23.15192557020386 25.425498428926375 26.55851708097027 24.864058919899495 34 23.80803963929595 25.42336633895413 26.68448941978833 24.084104601961588 35 24.11994324409045 25.74741146374324 26.874408182277744 23.25823710988857 36 23.587295087436846 25.788344336111123 26.274786359702134 24.349574216749893 37 23.518433463982163 25.281476565076105 26.967878355641073 24.23221161530066 38 23.235972231399792 24.70732589369562 27.56112018379592 24.495581691108665 39 23.613303329999027 23.70792906357632 27.36072000190098 25.318047604523674 40 23.41962492794024 24.56681953697516 27.97518507680244 24.03837045828216 41 22.254673752800606 24.450693873219745 28.60895288992589 24.685679484053757 42 23.428641552708356 24.841712663930863 28.227520366341885 23.502125417018895 43 22.334098173140678 23.66157645104998 28.593360964785358 25.41096441102398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1836.0 1 1669.5 2 1503.0 3 3607.0 4 5711.0 5 5711.0 6 8629.0 7 11547.0 8 11495.0 9 11443.0 10 14600.5 11 17758.0 12 17758.0 13 27825.0 14 37892.0 15 52061.5 16 66231.0 17 59837.5 18 53444.0 19 53444.0 20 55195.0 21 56946.0 22 50708.0 23 44470.0 24 50511.5 25 56553.0 26 56553.0 27 64897.5 28 73242.0 29 81451.5 30 89661.0 31 101801.5 32 113942.0 33 113942.0 34 135339.0 35 156736.0 36 189299.5 37 221863.0 38 255127.0 39 288391.0 40 288391.0 41 313735.5 42 339080.0 43 361752.5 44 384425.0 45 423968.5 46 463512.0 47 463512.0 48 554734.0 49 645956.0 50 638129.5 51 630303.0 52 551191.5 53 472080.0 54 472080.0 55 450033.0 56 427986.0 57 412255.0 58 396524.0 59 370401.5 60 344279.0 61 344279.0 62 287594.0 63 230909.0 64 200795.5 65 170682.0 66 144494.5 67 118307.0 68 118307.0 69 99127.5 70 79948.0 71 68498.5 72 57049.0 73 42271.5 74 27494.0 75 27494.0 76 21547.0 77 15600.0 78 13833.5 79 12067.0 80 9681.0 81 7295.0 82 7295.0 83 6185.5 84 5076.0 85 4521.0 86 3966.0 87 2663.5 88 1361.0 89 1361.0 90 981.5 91 602.0 92 433.5 93 265.0 94 253.5 95 242.0 96 242.0 97 129.5 98 17.0 99 14.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 6144206.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.47279611001171 #Duplication Level Percentage of deduplicated Percentage of total 1 81.12090174358669 36.076733235028236 2 9.509628142110312 8.458395068922028 3 3.2460755920933773 4.33086173894563 4 1.616549816875607 2.875699616303425 5 0.9152608641562751 2.035210489954757 6 0.6357079533605094 1.6963026117188862 7 0.463361563323071 1.4424889031618249 8 0.3533132002269391 1.2570260733334722 9 0.2654382318865525 1.0624302329843394 >10 1.653944471168084 13.61531039640541 >50 0.12210522356213595 3.7186444915241896 >100 0.0738716697748601 6.738820984589124 >500 0.01177314653581821 3.66473105635413 >1k 0.011367160405877968 9.78243718586926 >5k 4.4287637944661565E-4 1.3325952130053285 >10k+ 2.583445546771925E-4 1.9123127018998747 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 26844 0.43689941385428804 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 17451 0.28402368019561847 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 17045 0.27741582883125987 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16659 0.27113348738632786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 16391 0.26677165446601236 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11631 0.1893002936424983 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA 10489 0.1707136772432435 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG 9434 0.15354302899349404 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC 8124 0.13222212927105634 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT 7783 0.12667218514483403 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7387 0.12022708874018873 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 6977 0.11355413539194488 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6720 0.10937133292731396 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6700 0.10904582300788743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.627549597132648E-5 2 0.0 0.0 0.0 0.0 1.627549597132648E-5 3 0.0 0.0 0.0 0.0 1.627549597132648E-5 4 0.0 0.0 0.0 0.0 1.627549597132648E-5 5 0.0 0.0 0.0 0.0 1.627549597132648E-5 6 0.0 0.0 0.0 0.0 1.627549597132648E-5 7 1.627549597132648E-5 0.0 0.0 0.0 1.627549597132648E-5 8 1.627549597132648E-5 0.0 0.0 0.0 3.255099194265296E-5 9 1.627549597132648E-5 0.0 0.0 1.627549597132648E-5 9.765297582795889E-5 10 1.627549597132648E-5 0.0 0.0 3.255099194265296E-5 9.765297582795889E-5 11 1.627549597132648E-5 0.0 0.0 6.510198388530592E-5 9.765297582795889E-5 12 1.627549597132648E-5 0.0 0.0 1.1392847179928537E-4 9.765297582795889E-5 13 1.627549597132648E-5 1.627549597132648E-5 0.0 1.4647946374193833E-4 9.765297582795889E-5 14 1.627549597132648E-5 1.627549597132648E-5 0.0 1.627549597132648E-4 1.627549597132648E-4 15 1.627549597132648E-5 1.627549597132648E-5 0.0 3.255099194265296E-4 1.790304556845913E-4 16 1.627549597132648E-5 1.627549597132648E-5 0.0 7.812238066236711E-4 2.4413243956989723E-4 17 1.627549597132648E-5 1.627549597132648E-5 0.0 0.001415968149505404 2.4413243956989723E-4 18 1.627549597132648E-5 1.627549597132648E-5 0.0 0.0017414780689319336 2.766834315125502E-4 19 1.627549597132648E-5 1.627549597132648E-5 0.0 0.0026203548513835636 2.766834315125502E-4 20 1.627549597132648E-5 1.627549597132648E-5 0.0 0.0036619865935484585 2.766834315125502E-4 21 1.627549597132648E-5 1.627549597132648E-5 0.0 0.005452291150394371 2.766834315125502E-4 22 1.627549597132648E-5 1.627549597132648E-5 0.0 0.008040095009835282 2.766834315125502E-4 23 1.627549597132648E-5 1.627549597132648E-5 0.0 0.011978765034896291 4.0688739928316207E-4 24 1.627549597132648E-5 1.627549597132648E-5 0.0 0.01826110647982831 4.0688739928316207E-4 25 1.627549597132648E-5 1.627549597132648E-5 0.0 0.023029826799426972 4.0688739928316207E-4 26 1.627549597132648E-5 1.627549597132648E-5 0.0 0.031737217144086644 4.0688739928316207E-4 27 1.627549597132648E-5 1.627549597132648E-5 0.0 0.07366289476622366 4.0688739928316207E-4 28 1.627549597132648E-5 1.627549597132648E-5 0.0 0.13977395940175183 4.0688739928316207E-4 29 1.627549597132648E-5 1.627549597132648E-5 0.0 0.21519460773287874 4.0688739928316207E-4 30 1.627549597132648E-5 1.627549597132648E-5 0.0 0.32777221336654405 4.0688739928316207E-4 31 1.627549597132648E-5 1.627549597132648E-5 0.0 0.6570092213705074 4.0688739928316207E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGACG 2000 0.0 17.02 4 AAGACGG 1990 0.0 16.919598 5 CTTATAC 4495 0.0 16.051168 37 TCTAGCG 1370 0.0 14.3138685 28 TCTTATA 7145 0.0 14.240726 37 CGCAAGA 2340 0.0 14.072649 2 ACGGACC 2370 0.0 14.050632 8 CTAGCGG 1400 0.0 14.007142 29 GCGCAAG 2385 0.0 13.962263 1 GACGGAC 2360 0.0 13.875 7 GCAAGAC 2915 0.0 13.391081 3 AGACGGA 2540 0.0 13.32874 6 CGGACCA 2465 0.0 13.283977 9 TATACTG 575 0.0 13.191303 5 CGCAATA 1595 0.0 12.990595 36 TAGCGGC 1525 0.0 12.980328 30 CGGTCCA 1615 0.0 12.944273 10 ACCGTCG 1635 0.0 12.899082 8 TCTAGAT 1350 0.0 12.881482 2 TTAATAC 635 0.0 12.818897 3 >>END_MODULE