FastQCFastQC Report
Thu 9 Feb 2017
SRR2727166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4655911
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT277170.5953077711322231No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG228760.4913324159332083No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC215580.4630243146829911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT178700.3838131785594699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167380.3594999990334867No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA137980.2963544621020462No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130410.28009556024588955No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG125360.26924913298385644No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG103350.22197589258042089No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT92220.19807079645637557No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC87580.1881049702195768No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC84510.1815112015672121No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG81990.17609872697308862No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT81570.17519664787406802No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG74150.1592599171247045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT73180.15717654396744268No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60480.12989939025896327No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA59740.12831001279878415No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC57790.12412178841047433No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.11428483061639279No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC52880.11357605418144806No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT51150.10986034741643472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT50560.10859314106304867No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT50530.10852870684169005No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT47350.1016986793776771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG14050.016.0640565
CAAGACG13900.015.5719414
CTTATAC43100.014.98027837
TACGGAT11850.014.83122427
GTATACG1001.0945024E-414.7999991
CGAGCCG13400.014.63432915
CGTTACG5000.014.42999924
GTTACGG9100.014.23076925
CGGATGG13900.013.70863229
ACGGATG12700.013.69291228
ATACCGT10850.013.6405536
TTACGGA13150.013.22433526
CCCGTTA1409.5729865E-613.21428522
TATTCCG1409.5729865E-613.2142855
CTGTACG1551.8908468E-613.1290329
GACGGAC16350.013.0122327
AGACGGA17350.012.9020176
TACCGTC11050.012.8914037
TACTTAC13000.012.80769331
TCTTATA66550.012.7595837
Produced by FastQC (version 0.11.5)