##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727166.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4655911
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.106323767786797	31.0	31.0	33.0	30.0	34.0
2	31.252729487311935	31.0	31.0	34.0	30.0	34.0
3	31.30497962697311	31.0	31.0	34.0	28.0	34.0
4	35.14190090832922	35.0	35.0	37.0	32.0	37.0
5	34.99412145120471	35.0	35.0	37.0	32.0	37.0
6	35.02415209397259	36.0	35.0	37.0	32.0	37.0
7	34.8474021518023	35.0	35.0	37.0	32.0	37.0
8	34.779837071627874	35.0	35.0	37.0	32.0	37.0
9	36.3909020168126	38.0	35.0	39.0	32.0	39.0
10	36.17671085207599	38.0	35.0	39.0	32.0	39.0
11	36.28782530422081	38.0	35.0	39.0	32.0	39.0
12	36.159491880321596	38.0	35.0	39.0	31.0	39.0
13	36.25970814304655	38.0	35.0	39.0	32.0	39.0
14	37.215301151589884	39.0	36.0	40.0	31.0	41.0
15	37.111938995397466	39.0	36.0	40.0	31.0	41.0
16	37.07088000608259	39.0	36.0	40.0	31.0	41.0
17	36.99138106377033	39.0	36.0	40.0	31.0	41.0
18	36.990474903837296	39.0	36.0	40.0	31.0	41.0
19	37.01774067416667	39.0	36.0	40.0	31.0	41.0
20	36.90056790174898	39.0	36.0	40.0	31.0	41.0
21	36.77619653812111	39.0	35.0	40.0	30.0	41.0
22	36.72514831146901	39.0	35.0	40.0	30.0	41.0
23	36.50539475518325	38.0	35.0	40.0	30.0	41.0
24	36.39193189045066	38.0	35.0	40.0	30.0	41.0
25	36.2637009169634	38.0	35.0	40.0	29.0	41.0
26	35.949988090408084	38.0	34.0	40.0	28.0	41.0
27	36.081711398692974	38.0	34.0	40.0	29.0	41.0
28	35.94966720798572	38.0	34.0	40.0	28.0	41.0
29	35.84353738720521	38.0	34.0	40.0	27.0	41.0
30	35.96695168786517	38.0	34.0	40.0	29.0	41.0
31	35.890473636630944	38.0	34.0	40.0	28.0	41.0
32	35.711942088240086	38.0	34.0	40.0	27.0	41.0
33	35.62203186444071	38.0	34.0	40.0	27.0	41.0
34	35.644522629405934	38.0	34.0	40.0	27.0	41.0
35	35.35451794503804	38.0	34.0	40.0	26.0	41.0
36	35.201690711012304	38.0	34.0	40.0	25.0	41.0
37	35.004496219966406	38.0	33.0	40.0	25.0	41.0
38	34.85785767812143	38.0	33.0	40.0	24.0	41.0
39	34.78627319121865	38.0	33.0	40.0	24.0	41.0
40	34.6504510073324	38.0	33.0	40.0	24.0	41.0
41	34.471521470234286	38.0	33.0	40.0	23.0	41.0
42	34.304202335482785	38.0	33.0	40.0	23.0	41.0
43	33.882788781830236	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	6.0
13	10.0
14	22.0
15	56.0
16	174.0
17	343.0
18	733.0
19	1538.0
20	3064.0
21	5581.0
22	9759.0
23	15944.0
24	25119.0
25	37827.0
26	54016.0
27	75065.0
28	101012.0
29	129406.0
30	162015.0
31	195647.0
32	234170.0
33	276993.0
34	331029.0
35	392665.0
36	477030.0
37	596886.0
38	776188.0
39	753610.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.731145634012336	19.726493912791717	12.913240824405792	25.629119628790153
2	17.54889644582983	23.971807021225278	33.61735222172417	24.861944311220725
3	20.843933657666565	25.44112204893951	29.741010942863817	23.97393335053011
4	15.476004588575684	16.93393623718323	33.29372060591365	34.29633856832744
5	14.114466535120624	37.84241150657734	33.34380747398307	14.699314484318965
6	33.90187226517002	33.957564910497645	16.3310037498569	15.809559074475436
7	26.797419452390734	31.55511348906798	22.585848397875303	19.061618660665978
8	27.34251148701081	33.01549363808716	21.68142818881203	17.960566686090004
9	27.847740216683697	13.841007699674671	19.936592430568368	38.374659653073266
10	17.4756132580713	26.879250913516174	32.914288954406565	22.730846874005966
11	35.01042008749738	22.319606195221514	20.91536973107948	21.754603986201626
12	21.459581164674326	26.62462834878072	29.530955381234737	22.384835105310216
13	30.51615462580792	19.145619407243824	25.476260177653742	24.861965789294512
14	22.64645952209997	21.62824847811739	25.86939484023642	29.855897159546217
15	26.65132559449697	26.46631346690261	23.222243724160535	23.66011721443988
16	23.453090061214656	25.828844236928067	27.062115233731916	23.655950468125358
17	24.00284283784634	26.838657354060246	26.360233260472548	22.798266547620862
18	24.43974981480531	23.672767799899955	28.571873474385573	23.315608910909162
19	24.061778672315686	27.161129153886314	27.37137801817947	21.405714155618526
20	23.602942582021004	24.535885673072357	27.95603266471374	23.9051390801929
21	23.63200241585374	26.59765188810525	27.27071028634353	22.49963540969748
22	24.027864793807268	25.896478691280826	25.465821833793644	24.609834681118258
23	21.92694405026213	25.58902006503131	27.138512742189448	25.345523142517113
24	22.581703129634565	27.96150957352922	26.534141223919445	22.922646072916773
25	23.98140772020771	25.39268899255162	25.234889584444375	25.391013702796293
26	23.184893353846327	26.10820954266523	27.148585958795174	23.558311144693274
27	24.749979112573243	25.676113654234374	25.58279142363331	23.991115809559073
28	22.719313148382778	26.429564482654417	27.298417001527735	23.552705367435074
29	23.264791788331003	25.758653891794754	27.705684236661742	23.270870083212504
30	23.117495158305218	26.94353478835828	27.7309639295081	22.208006123828397
31	24.467220271177865	25.86814911195682	25.457874946492748	24.206755670372566
32	21.596718665799237	26.7383117933311	26.834791300778733	24.83017824009093
33	22.46975940906087	25.86456227363453	27.483128436089093	24.18254988121551
34	22.806793342913988	25.990123092988675	27.6445576386662	23.558525925431134
35	23.041527211323412	26.577763191779226	28.050557667446824	22.330151929450544
36	23.24988600512338	26.17932344497135	26.936081896754473	23.634708653150803
37	22.539455758497102	26.03673051310474	27.903669120822972	23.52014460757519
38	22.943952322112686	25.276020095744954	28.117483345364636	23.662544236777723
39	22.93121582435747	23.868948525863146	28.117826994545215	25.082008655234173
40	22.90166199482765	24.964695416213928	28.607398208427952	23.52624438053047
41	21.456123194794746	24.95006884796552	29.173281018473073	24.420526938766656
42	22.847752029624278	25.545569921761818	28.708065940263893	22.898612108350008
43	21.68933211996535	23.920044863400523	29.11290615306006	25.277716863574067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1990.0
1	2061.0
2	2132.0
3	4883.0
4	7634.0
5	7634.0
6	11237.0
7	14840.0
8	14578.5
9	14317.0
10	17710.0
11	21103.0
12	21103.0
13	31736.0
14	42369.0
15	56331.5
16	70294.0
17	62850.0
18	55406.0
19	55406.0
20	56211.5
21	57017.0
22	48502.0
23	39987.0
24	43679.0
25	47371.0
26	47371.0
27	52447.5
28	57524.0
29	62609.0
30	67694.0
31	75582.0
32	83470.0
33	83470.0
34	98397.0
35	113324.0
36	136411.0
37	159498.0
38	182022.0
39	204546.0
40	204546.0
41	220587.0
42	236628.0
43	255385.5
44	274143.0
45	312681.5
46	351220.0
47	351220.0
48	452988.0
49	554756.0
50	549175.5
51	543595.0
52	443837.5
53	344080.0
54	344080.0
55	319454.5
56	294829.0
57	283360.0
58	271891.0
59	251876.0
60	231861.0
61	231861.0
62	192576.0
63	153291.0
64	133381.0
65	113471.0
66	96316.5
67	79162.0
68	79162.0
69	66636.0
70	54110.0
71	46616.0
72	39122.0
73	29372.0
74	19622.0
75	19622.0
76	15451.5
77	11281.0
78	9877.0
79	8473.0
80	6913.0
81	5353.0
82	5353.0
83	4553.0
84	3753.0
85	3349.0
86	2945.0
87	1972.5
88	1000.0
89	1000.0
90	722.0
91	444.0
92	312.0
93	180.0
94	171.5
95	163.0
96	163.0
97	84.5
98	6.0
99	11.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4655911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.61809373021276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38752002846633	36.75974090829892
2	8.965601924964062	8.00056134071645
3	3.0196989416792297	4.041996312506045
4	1.5313601432843722	2.733054816310967
5	0.8938006815399135	1.9939841292537959
6	0.589050793287448	1.5769394104053325
7	0.41550796859727995	1.2977421441966617
8	0.31036764886691237	1.1078410278375728
9	0.2474545047407177	0.9936853457836249
>10	1.4191577796274457	11.596966749970523
>50	0.10806571733176622	3.313872520165607
>100	0.08656779923858293	8.027632971570501
>500	0.014629850097342138	4.545336001728641
>1k	0.010045830284272124	8.40514755632396
>5k	7.314924964275816E-4	2.200962732628151
>10k+	4.388954978565489E-4	3.4045360323031626
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27717	0.5953077711322231	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	22876	0.4913324159332083	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	21558	0.4630243146829911	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17870	0.3838131785594699	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16738	0.3594999990334867	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	13798	0.2963544621020462	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13041	0.28009556024588955	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	12536	0.26924913298385644	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	10335	0.22197589258042089	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	9222	0.19807079645637557	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	8758	0.1881049702195768	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	8451	0.1815112015672121	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	8199	0.17609872697308862	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	8157	0.17519664787406802	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	7415	0.1592599171247045	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	7318	0.15717654396744268	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	6048	0.12989939025896327	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5974	0.12831001279878415	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	5779	0.12412178841047433	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5321	0.11428483061639279	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	5288	0.11357605418144806	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5115	0.10986034741643472	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	5056	0.10859314106304867	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	5053	0.10852870684169005	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	4735	0.1016986793776771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1478073786204248E-5
2	0.0	0.0	0.0	0.0	2.1478073786204248E-5
3	0.0	0.0	0.0	0.0	2.1478073786204248E-5
4	0.0	0.0	0.0	2.1478073786204248E-5	2.1478073786204248E-5
5	0.0	0.0	0.0	2.1478073786204248E-5	2.1478073786204248E-5
6	0.0	0.0	0.0	2.1478073786204248E-5	4.2956147572408496E-5
7	0.0	0.0	0.0	4.2956147572408496E-5	6.443422135861274E-5
8	2.1478073786204248E-5	0.0	0.0	4.2956147572408496E-5	6.443422135861274E-5
9	2.1478073786204248E-5	0.0	0.0	8.591229514481699E-5	1.2886844271722548E-4
10	2.1478073786204248E-5	0.0	0.0	1.5034651650342972E-4	1.2886844271722548E-4
11	2.1478073786204248E-5	0.0	0.0	1.9330266407583822E-4	1.2886844271722548E-4
12	2.1478073786204248E-5	0.0	0.0	2.7921495922065523E-4	1.5034651650342972E-4
13	2.1478073786204248E-5	0.0	0.0	2.7921495922065523E-4	1.5034651650342972E-4
14	8.591229514481699E-5	0.0	0.0	4.0808340193788066E-4	1.9330266407583822E-4
15	8.591229514481699E-5	0.0	0.0	7.087764349447401E-4	2.5773688543445097E-4
16	8.591229514481699E-5	0.0	0.0	0.0015034651650342973	3.221711067930637E-4
17	8.591229514481699E-5	0.0	0.0	0.0026418030757031224	3.221711067930637E-4
18	8.591229514481699E-5	0.0	0.0	0.0033505795106478623	3.221711067930637E-4
19	8.591229514481699E-5	0.0	0.0	0.0054983868892682876	3.4364918057926797E-4
20	8.591229514481699E-5	0.0	0.0	0.007238110865950831	3.4364918057926797E-4
21	8.591229514481699E-5	0.0	0.0	0.011490769475619273	3.651272543654722E-4
22	8.591229514481699E-5	0.0	0.0	0.017203937102749603	3.651272543654722E-4
23	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.02448500411627284	4.510395495102892E-4
24	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.03737184838799539	4.510395495102892E-4
25	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.04557647257432541	4.510395495102892E-4
26	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.06226493590620611	4.510395495102892E-4
27	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.12117929230176436	4.510395495102892E-4
28	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.21566133888727684	4.725176232964934E-4
29	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.31089511805530645	4.725176232964934E-4
30	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.45022338270641343	4.725176232964934E-4
31	8.591229514481699E-5	2.1478073786204248E-5	0.0	0.820011379083492	4.725176232964934E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1405	0.0	16.064056	5
CAAGACG	1390	0.0	15.571941	4
CTTATAC	4310	0.0	14.980278	37
TACGGAT	1185	0.0	14.831224	27
GTATACG	100	1.0945024E-4	14.799999	1
CGAGCCG	1340	0.0	14.634329	15
CGTTACG	500	0.0	14.429999	24
GTTACGG	910	0.0	14.230769	25
CGGATGG	1390	0.0	13.708632	29
ACGGATG	1270	0.0	13.692912	28
ATACCGT	1085	0.0	13.640553	6
TTACGGA	1315	0.0	13.224335	26
CCCGTTA	140	9.5729865E-6	13.214285	22
TATTCCG	140	9.5729865E-6	13.214285	5
CTGTACG	155	1.8908468E-6	13.129032	9
GACGGAC	1635	0.0	13.012232	7
AGACGGA	1735	0.0	12.902017	6
TACCGTC	1105	0.0	12.891403	7
TACTTAC	1300	0.0	12.807693	31
TCTTATA	6655	0.0	12.75958	37
>>END_MODULE