FastQCFastQC Report
Thu 9 Feb 2017
SRR2727165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9272954
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG592970.6394618155120795No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC591330.6376932313047169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT581280.6268552610095984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT375160.40457442148424333No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA367130.3959148292981935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT342760.3696340993387867No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG325760.35130121426246697No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG270900.2921399157161785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245140.2643602028005315No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT233140.2514193427466587No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC229820.24783903813175392No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG210430.22692876509470444No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT206320.22249652052625302No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC205830.2219681020740532No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG190340.2052636085545124No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT188160.2029126856447255No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA171560.18501116257020148No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155990.16822039665030153No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC155460.16764884199792213No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT130580.14081812548622585No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC121530.13105856019559678No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT121460.13098307184528254No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT121430.13095071969514785No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC117930.12717630217943496No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT113530.1224313201596816No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC112520.12134213110514729No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT109600.11819318849203825No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT104540.11273645916932187No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC104030.11218647261703228No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA97800.10546800943906333No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95450.10293375767851325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG29250.017.3931645
CAAGACG31450.016.176474
CTTATAC65650.015.893373537
TAGTACT5700.014.9298244
TCGTTAC7400.014.523
TATACCG3150.014.0952385
CGCAAGA35700.014.0434172
TCTAGAT17250.013.9420292
TCTAGCG19650.013.93384228
TCTTATA103250.013.814527537
GCGCAAG36700.013.8119881
GTTACGG8850.013.7966125
CTAGCGG20000.013.78249929
GACGGAC35850.013.7266397
ACCGTCG22300.013.5224238
AGTACTC29700.013.5168355
ACGAACG13300.013.4924815
ATACCGT23200.013.3965516
ACGGACC36650.013.3765348
TACCGTC22750.013.3362647
Produced by FastQC (version 0.11.5)