##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9272954
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152803087344118	31.0	31.0	33.0	30.0	34.0
2	31.283440746066464	31.0	31.0	34.0	30.0	34.0
3	31.339169912845463	31.0	31.0	34.0	28.0	34.0
4	35.17328566495639	35.0	35.0	37.0	33.0	37.0
5	35.028661093325816	35.0	35.0	37.0	32.0	37.0
6	35.066035591247406	36.0	35.0	37.0	32.0	37.0
7	34.88131980380793	36.0	35.0	37.0	32.0	37.0
8	34.81669951128842	36.0	35.0	37.0	32.0	37.0
9	36.4331966922299	38.0	35.0	39.0	32.0	39.0
10	36.21698144949279	38.0	35.0	39.0	32.0	39.0
11	36.3314432488288	38.0	35.0	39.0	32.0	39.0
12	36.20291236212322	38.0	35.0	39.0	32.0	39.0
13	36.29948245187025	38.0	35.0	39.0	32.0	39.0
14	37.261758982089205	39.0	36.0	41.0	32.0	41.0
15	37.152831125874236	39.0	36.0	41.0	31.0	41.0
16	37.10880567292796	39.0	36.0	40.0	31.0	41.0
17	37.04221082084522	39.0	36.0	40.0	31.0	41.0
18	37.0463130734823	39.0	36.0	40.0	31.0	41.0
19	37.06272132914711	39.0	36.0	40.0	31.0	41.0
20	36.96482199739155	39.0	36.0	40.0	31.0	41.0
21	36.83794128602385	39.0	36.0	40.0	30.0	41.0
22	36.7986066791661	39.0	35.0	40.0	30.0	41.0
23	36.58733462928858	38.0	35.0	40.0	30.0	41.0
24	36.47500063086693	38.0	35.0	40.0	30.0	41.0
25	36.3585872419943	38.0	35.0	40.0	30.0	41.0
26	36.04510957349729	38.0	34.0	40.0	29.0	41.0
27	36.1773007824691	38.0	35.0	40.0	29.0	41.0
28	36.04865288882054	38.0	35.0	40.0	29.0	41.0
29	35.950853525208906	38.0	34.0	40.0	28.0	41.0
30	36.06691438348557	38.0	35.0	40.0	29.0	41.0
31	35.99688211545102	38.0	35.0	40.0	29.0	41.0
32	35.80584547275873	38.0	34.0	40.0	28.0	41.0
33	35.73035809300898	38.0	34.0	40.0	27.0	41.0
34	35.745014695425	38.0	34.0	40.0	28.0	41.0
35	35.465214428972686	38.0	34.0	40.0	27.0	41.0
36	35.32881086221284	38.0	34.0	40.0	26.0	41.0
37	35.12922958530798	38.0	33.0	40.0	25.0	41.0
38	34.97981872874598	38.0	33.0	40.0	25.0	41.0
39	34.90997108364821	38.0	33.0	40.0	24.0	41.0
40	34.76624406850287	38.0	33.0	40.0	24.0	41.0
41	34.59378651074943	38.0	33.0	40.0	24.0	41.0
42	34.41994751618524	38.0	33.0	40.0	23.0	41.0
43	33.98356446068858	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	7.0
13	15.0
14	50.0
15	107.0
16	324.0
17	775.0
18	1375.0
19	2694.0
20	5500.0
21	10514.0
22	18433.0
23	30716.0
24	48011.0
25	72050.0
26	103211.0
27	144536.0
28	193392.0
29	251568.0
30	315165.0
31	384087.0
32	458164.0
33	544796.0
34	651350.0
35	777129.0
36	942733.0
37	1191212.0
38	1550434.0
39	1574601.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.44187990148555	19.305423061518475	12.595533203335204	25.657163833660775
2	17.162147035346017	24.055236335691948	33.43152570367544	25.35109092528659
3	20.85428224921638	25.621425491811994	29.43710278299666	24.08718947597497
4	15.14052587773001	16.892890873825106	33.073290345234106	34.89329290321078
5	13.95780675715635	37.9072299938078	33.37816622405331	14.756797024982546
6	33.93590650832518	33.58895126623081	16.471428629970557	16.00371359547346
7	26.89050328514517	31.281121420423307	22.524386511569023	19.303988782862504
8	27.797183076719673	32.48105188486862	21.750846601848774	17.970918436562933
9	28.045787782404613	13.568933912537473	19.698307572754054	38.68697073230386
10	17.590381662628758	26.80584849229275	32.67810883133897	22.925661013739525
11	35.05442817898158	22.214474481378858	20.66015856435824	22.070938775281316
12	21.775628348851942	26.77719527132346	29.180862969879932	22.26631340994466
13	30.77940427613466	18.9195050466119	25.247229739304217	25.053860937949224
14	22.89107656524555	21.52869517092396	25.611159076169255	29.969069187661233
15	27.096392368602277	25.88595823941324	23.115708327680693	23.90194106430378
16	23.553044693201326	25.449096372094587	27.22862639025277	23.769232544451317
17	24.405868938851633	26.641111343806944	26.24644746431396	22.70657225302746
18	24.91769073803235	22.876561233885123	28.651366112675635	23.554381915406893
19	24.258418622587797	27.105019608638198	27.317282065671844	21.31927970310216
20	23.93748529325175	24.149294820183513	27.99540470059487	23.917815185969864
21	23.799546509127513	26.28879642884026	27.183883366616506	22.72777369541572
22	24.089896272536237	25.860313768406485	25.071988926074688	24.977801032982587
23	21.8180635857786	25.359297587370754	26.997836935242	25.82480189160865
24	22.6664663709105	28.17947765081116	26.206621967498165	22.947434010780167
25	24.157652458968307	25.062197008633923	24.822446008035843	25.957704524361922
26	23.451599134429006	25.844245533839594	26.96661711036203	23.737538221369373
27	24.96178671866592	25.304395988592198	25.281091656445188	24.452725636296694
28	22.863275284229815	26.126927837666404	27.374804188611314	23.634992689492474
29	23.54100969335122	25.25448740498443	27.59479880952715	23.6097040921372
30	22.973261810637688	27.084033847250833	27.855179697861114	22.087524644250365
31	25.03686527507847	25.504386196674762	24.885317019797576	24.573431508449197
32	21.36306294628443	26.692109116469247	26.710916499747544	25.233911437498772
33	22.5543122504436	25.89736776436074	27.33716785395463	24.211152131241025
34	22.73455686289396	25.89388451619624	27.349030309004014	24.022528311905784
35	22.965227693354244	26.851076798181033	27.996288992698553	22.187406515766174
36	23.60704043177611	26.211485574068416	26.478444732929766	23.703029261225712
37	22.782890975195176	26.22139611605967	27.44048983743476	23.555223071310394
38	23.23279075901811	25.527237598719886	27.707837222097726	23.532134420164276
39	23.425609573820815	23.768941375100102	27.360892764053396	25.444556287025687
40	23.16673845249313	25.26374011992295	28.04437507184873	23.525146355735185
41	21.788418232205185	25.089566927647866	28.518905625974206	24.603109214172743
42	23.511224147127226	25.692481597557805	28.039457545028263	22.756836710286713
43	22.374822521496387	23.73181189079553	28.649921049969624	25.24344453773846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1763.0
1	2074.5
2	2386.0
3	7638.5
4	12891.0
5	12891.0
6	19623.5
7	26356.0
8	26322.0
9	26288.0
10	32384.0
11	38480.0
12	38480.0
13	58480.5
14	78481.0
15	108949.5
16	139418.0
17	124307.5
18	109197.0
19	109197.0
20	110481.5
21	111766.0
22	93805.0
23	75844.0
24	82974.0
25	90104.0
26	90104.0
27	99766.5
28	109429.0
29	118192.0
30	126955.0
31	140750.0
32	154545.0
33	154545.0
34	182589.0
35	210633.0
36	254702.0
37	298771.0
38	342028.5
39	385286.0
40	385286.0
41	419005.0
42	452724.0
43	489612.0
44	526500.0
45	602898.0
46	679296.0
47	679296.0
48	928383.5
49	1177471.0
50	1167759.5
51	1158048.0
52	915047.5
53	672047.0
54	672047.0
55	627286.5
56	582526.0
57	562265.5
58	542005.0
59	506403.5
60	470802.0
61	470802.0
62	391223.0
63	311644.0
64	271263.5
65	230883.0
66	196715.5
67	162548.0
68	162548.0
69	137808.0
70	113068.0
71	97972.5
72	82877.0
73	61952.5
74	41028.0
75	41028.0
76	32428.5
77	23829.0
78	20896.5
79	17964.0
80	14771.0
81	11578.0
82	11578.0
83	9687.5
84	7797.0
85	6834.0
86	5871.0
87	3979.0
88	2087.0
89	2087.0
90	1525.5
91	964.0
92	687.5
93	411.0
94	382.0
95	353.0
96	353.0
97	188.5
98	24.0
99	20.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9272954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.308761526135605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03377653986618	28.69618544895893
2	10.095400421199288	7.331029728083478
3	3.67053122142495	3.9981732837886135
4	1.8621065566373611	2.7044313160479785
5	1.1038913499924163	2.0040463888819273
6	0.717844087915791	1.563841788064847
7	0.5200928279552489	1.321874852317643
8	0.38791010936724035	1.126762852367385
9	0.3129501677332078	1.0226549708810264
>10	2.0017289986319597	13.728978534439538
>50	0.15944881512435563	3.9953276686838213
>100	0.10298998982268621	7.47627678679107
>500	0.014764559951251926	3.7370174280838877
>1k	0.014223739092911539	10.329134011367168
>5k	0.0014703865253674674	3.559001759684909
>10k+	8.702287599113582E-4	7.405263181557956
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	59297	0.6394618155120795	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	59133	0.6376932313047169	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	58128	0.6268552610095984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	37516	0.40457442148424333	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	36713	0.3959148292981935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34276	0.3696340993387867	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	32576	0.35130121426246697	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	27090	0.2921399157161785	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24514	0.2643602028005315	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	23314	0.2514193427466587	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	22982	0.24783903813175392	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	21043	0.22692876509470444	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	20632	0.22249652052625302	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	20583	0.2219681020740532	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	19034	0.2052636085545124	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	18816	0.2029126856447255	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	17156	0.18501116257020148	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	15599	0.16822039665030153	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	15546	0.16764884199792213	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	13058	0.14081812548622585	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	12153	0.13105856019559678	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	12146	0.13098307184528254	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	12143	0.13095071969514785	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	11793	0.12717630217943496	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	11353	0.1224313201596816	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	11252	0.12134213110514729	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	10960	0.11819318849203825	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	10454	0.11273645916932187	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	10403	0.11218647261703228	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9780	0.10546800943906333	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9545	0.10293375767851325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0784050044894E-5
2	0.0	0.0	0.0	1.0784050044894E-5	1.0784050044894E-5
3	0.0	0.0	0.0	1.0784050044894E-5	1.0784050044894E-5
4	0.0	0.0	0.0	1.0784050044894E-5	1.0784050044894E-5
5	0.0	0.0	0.0	1.0784050044894E-5	1.0784050044894E-5
6	0.0	0.0	0.0	1.0784050044894E-5	5.392025022447E-5
7	0.0	0.0	0.0	2.1568100089788E-5	5.392025022447E-5
8	0.0	0.0	0.0	2.1568100089788E-5	6.4704300269364E-5
9	0.0	0.0	0.0	4.3136200179576E-5	9.7056450404046E-5
10	0.0	0.0	0.0	6.4704300269364E-5	9.7056450404046E-5
11	0.0	0.0	0.0	1.0784050044894E-4	9.7056450404046E-5
12	0.0	0.0	0.0	1.72544800718304E-4	9.7056450404046E-5
13	0.0	0.0	0.0	2.04896950852986E-4	9.7056450404046E-5
14	0.0	0.0	0.0	2.91169351212138E-4	1.50976700628516E-4
15	2.1568100089788E-5	0.0	0.0	5.9312275246917E-4	2.04896950852986E-4
16	2.1568100089788E-5	0.0	0.0	0.00134800625561175	2.6960125112235003E-4
17	3.2352150134682E-5	0.0	0.0	0.002070537608619648	2.6960125112235003E-4
18	3.2352150134682E-5	0.0	0.0	0.00253425176055009	2.80385301167244E-4
19	3.2352150134682E-5	0.0	0.0	0.00415185926728419	2.80385301167244E-4
20	3.2352150134682E-5	1.0784050044894E-5	0.0	0.005640058173479562	3.01953401257032E-4
21	3.2352150134682E-5	1.0784050044894E-5	0.0	0.00959780453995566	3.34305551391714E-4
22	3.2352150134682E-5	1.0784050044894E-5	0.0	0.014321218459619232	3.34305551391714E-4
23	4.3136200179576E-5	1.0784050044894E-5	0.0	0.021740644890506304	4.63714151930442E-4
24	4.3136200179576E-5	1.0784050044894E-5	0.0	0.03296684098724096	4.63714151930442E-4
25	4.3136200179576E-5	1.0784050044894E-5	0.0	0.04039705146817293	4.63714151930442E-4
26	4.3136200179576E-5	1.0784050044894E-5	0.0	0.05475062207792684	4.63714151930442E-4
27	4.3136200179576E-5	1.0784050044894E-5	0.0	0.09177226588204794	4.74498201975336E-4
28	4.3136200179576E-5	1.0784050044894E-5	0.0	0.15848239945976222	4.74498201975336E-4
29	4.3136200179576E-5	1.0784050044894E-5	0.0	0.22477195508572564	4.74498201975336E-4
30	4.3136200179576E-5	1.0784050044894E-5	0.0	0.3267675004103331	4.74498201975336E-4
31	4.3136200179576E-5	1.0784050044894E-5	0.0	0.6063332137741652	5.17634402154912E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2925	0.0	17.393164	5
CAAGACG	3145	0.0	16.17647	4
CTTATAC	6565	0.0	15.8933735	37
TAGTACT	570	0.0	14.929824	4
TCGTTAC	740	0.0	14.5	23
TATACCG	315	0.0	14.095238	5
CGCAAGA	3570	0.0	14.043417	2
TCTAGAT	1725	0.0	13.942029	2
TCTAGCG	1965	0.0	13.933842	28
TCTTATA	10325	0.0	13.8145275	37
GCGCAAG	3670	0.0	13.811988	1
GTTACGG	885	0.0	13.79661	25
CTAGCGG	2000	0.0	13.782499	29
GACGGAC	3585	0.0	13.726639	7
ACCGTCG	2230	0.0	13.522423	8
AGTACTC	2970	0.0	13.516835	5
ACGAACG	1330	0.0	13.49248	15
ATACCGT	2320	0.0	13.396551	6
ACGGACC	3665	0.0	13.376534	8
TACCGTC	2275	0.0	13.336264	7
>>END_MODULE