##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727164.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5547764
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93715053488216	31.0	31.0	33.0	28.0	34.0
2	31.10375279121462	31.0	31.0	34.0	28.0	34.0
3	31.14185823333509	31.0	31.0	34.0	28.0	34.0
4	34.99860989039909	35.0	35.0	37.0	32.0	37.0
5	34.83696963317113	35.0	35.0	37.0	32.0	37.0
6	34.86761927868597	35.0	35.0	37.0	32.0	37.0
7	34.68577286272451	35.0	35.0	37.0	31.0	37.0
8	34.6093179161911	35.0	35.0	37.0	30.0	37.0
9	36.197323462209276	38.0	35.0	39.0	32.0	39.0
10	35.95322403764832	37.0	35.0	39.0	30.0	39.0
11	36.08411749310172	38.0	35.0	39.0	31.0	39.0
12	35.92785814248768	37.0	35.0	39.0	30.0	39.0
13	36.04164074030546	37.0	35.0	39.0	30.0	39.0
14	36.947119055533	39.0	36.0	40.0	31.0	41.0
15	36.8616556147666	38.0	36.0	40.0	31.0	41.0
16	36.83462454423079	38.0	36.0	40.0	31.0	41.0
17	36.70634745818315	38.0	36.0	40.0	30.0	41.0
18	36.7119637749551	38.0	36.0	40.0	30.0	41.0
19	36.71574511821339	39.0	35.0	40.0	30.0	41.0
20	36.57448676619986	38.0	35.0	40.0	30.0	41.0
21	36.43016051151419	38.0	35.0	40.0	30.0	41.0
22	36.349704313305324	38.0	35.0	40.0	30.0	41.0
23	36.12449141672212	38.0	34.0	40.0	29.0	41.0
24	35.97622771985254	38.0	34.0	40.0	28.0	41.0
25	35.83835595746322	38.0	34.0	40.0	27.0	41.0
26	35.51909003339003	38.0	34.0	40.0	27.0	41.0
27	35.657173232314854	38.0	34.0	40.0	27.0	41.0
28	35.50426604304004	38.0	34.0	40.0	27.0	41.0
29	35.385227814305004	38.0	34.0	40.0	27.0	41.0
30	35.532041557643765	38.0	34.0	40.0	27.0	41.0
31	35.48765394490465	38.0	34.0	40.0	27.0	41.0
32	35.34440019438462	38.0	34.0	40.0	26.0	41.0
33	35.218597078029994	38.0	33.0	40.0	26.0	41.0
34	35.27074998143396	38.0	34.0	40.0	26.0	41.0
35	34.94426943900281	38.0	33.0	40.0	25.0	41.0
36	34.791204168021565	38.0	33.0	40.0	25.0	41.0
37	34.60495940346417	38.0	33.0	40.0	24.0	41.0
38	34.43388741842659	37.0	33.0	40.0	24.0	41.0
39	34.36016420309155	37.0	33.0	40.0	24.0	41.0
40	34.20194947009281	37.0	33.0	40.0	23.0	41.0
41	33.99667019000808	37.0	32.0	40.0	23.0	41.0
42	33.811114531908714	37.0	32.0	40.0	22.0	41.0
43	33.39643791624878	37.0	31.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	13.0
13	26.0
14	36.0
15	155.0
16	358.0
17	645.0
18	1260.0
19	2520.0
20	5010.0
21	9053.0
22	15580.0
23	24534.0
24	38094.0
25	54951.0
26	76539.0
27	103332.0
28	134672.0
29	169993.0
30	209346.0
31	251092.0
32	295017.0
33	346925.0
34	409578.0
35	480022.0
36	576117.0
37	709631.0
38	913950.0
39	719311.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.23835548880594	21.111099895381276	13.317671768301608	25.33287284751118
2	19.616479720478377	23.509399462558246	32.40990424250202	24.46421657446135
3	21.214583028405677	24.344672916872454	29.833100326545974	24.607643728175894
4	15.871403325736278	16.635224569754588	33.798950351889516	33.69442175261961
5	14.777413026221014	37.489968931627224	32.85689513829355	14.875722903858202
6	34.70097141839487	35.06095428716867	15.185162887246104	15.052911407190356
7	28.020460134930037	31.353622828945138	21.596358460814123	19.029558575310702
8	27.659882431913108	32.5194078190781	20.51215588839035	19.308553860618442
9	27.531488361797653	13.432673055306607	19.6787750884861	39.35706349440964
10	17.57949689280222	26.33612388702908	32.19558366217453	23.888795557994175
11	36.621366734417684	21.73293600809263	20.537751786125007	21.10794547136468
12	21.780126191380887	26.233235588247805	28.431112066050396	23.555526154320912
13	30.574407995725846	18.660382813688543	25.079040853215815	25.686168337369796
14	23.127371676228478	21.277653483457478	24.565049991311817	31.029924849002228
15	26.240535826686212	27.32673920520051	21.852732019602854	24.579992948510426
16	24.924041469680397	25.5979886671459	24.896516866975595	24.58145299619811
17	24.344582790471982	26.375040466753813	25.199792204571065	24.08058453820314
18	24.85179614706033	23.459884018137757	27.24636448125767	24.44195535354424
19	24.777207538027934	25.801782483898016	26.307878273120487	23.113131704953563
20	24.65620383275136	24.128621909655852	26.22157323202645	24.993601025566335
21	25.38548864010798	25.60157569788477	25.588579470936402	23.424356191070853
22	25.024135850046974	25.030120243038457	25.134252286146275	24.811491620768294
23	23.273070015234968	24.51245222399511	26.22440320100134	25.990074559768583
24	23.960103566049312	26.352418740234807	25.64575565939719	24.041722034318692
25	24.926096351611207	24.87193038492625	24.82755214533279	25.374421118129753
26	24.30831592692119	25.88603264306124	25.796320824029284	24.009330605988286
27	25.50577133418076	25.042071003741327	24.890550499264208	24.561607162813704
28	23.804365146030005	25.566949134822607	26.48286769228107	24.14581802686632
29	23.99478420495176	25.463033395075925	26.46794276036255	24.07423963960976
30	23.900620141736383	26.20280891544774	26.42104098155581	23.47552996126007
31	24.994087708128895	25.73925278724906	24.74321185976909	24.523447644852954
32	23.335581686603827	25.81596837933265	25.237212686047926	25.611237248015595
33	23.371470019272632	25.113757542678457	26.265176384575838	25.249596053473073
34	24.423821921768845	25.308556744663257	26.211280797092307	24.056340536475595
35	24.79881984886163	25.53066424599172	26.471205336059718	23.199310569086933
36	23.786808523217644	26.01873475511936	25.838211574969666	24.356245146693333
37	23.974686017646025	25.487619877125272	26.402817423379943	24.13487668184876
38	23.66373191073016	24.625831235791573	27.212693258040538	24.49774359543773
39	24.17300014924932	23.8392260377334	26.675810290416102	25.31196352260118
40	24.142988057891433	24.676770677339555	27.260892857014106	23.91934840775491
41	22.855027719275732	24.560345393207065	27.981922086087295	24.60270480142991
42	24.263937687327726	24.846532765272638	27.53494921557586	23.354580331823776
43	23.063399957171935	23.647869664246713	27.862919186901248	25.425811191680108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	894.0
1	945.0
2	996.0
3	2106.0
4	3216.0
5	3216.0
6	5704.0
7	8192.0
8	8538.0
9	8884.0
10	12120.5
11	15357.0
12	15357.0
13	26311.5
14	37266.0
15	52482.0
16	67698.0
17	57990.5
18	48283.0
19	48283.0
20	49895.5
21	51508.0
22	44585.0
23	37662.0
24	42721.0
25	47780.0
26	47780.0
27	54872.5
28	61965.0
29	68338.5
30	74712.0
31	84139.0
32	93566.0
33	93566.0
34	114066.0
35	134566.0
36	165376.0
37	196186.0
38	226291.0
39	256396.0
40	256396.0
41	276703.5
42	297011.0
43	315855.5
44	334700.0
45	371116.0
46	407532.0
47	407532.0
48	481651.5
49	555771.0
50	550024.5
51	544278.0
52	487903.0
53	431528.0
54	431528.0
55	413081.5
56	394635.0
57	385479.5
58	376324.0
59	353472.5
60	330621.0
61	330621.0
62	273345.0
63	216069.0
64	190837.0
65	165605.0
66	141621.5
67	117638.0
68	117638.0
69	100025.5
70	82413.0
71	72971.0
72	63529.0
73	47228.0
74	30927.0
75	30927.0
76	24245.5
77	17564.0
78	15502.0
79	13440.0
80	11061.0
81	8682.0
82	8682.0
83	7395.5
84	6109.0
85	5476.5
86	4844.0
87	3376.5
88	1909.0
89	1909.0
90	1355.5
91	802.0
92	579.5
93	357.0
94	328.5
95	300.0
96	300.0
97	164.0
98	28.0
99	24.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5547764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.739714600315686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.65468713474331	34.89898023916999
2	9.299862013724637	7.949468965778163
3	3.140648641314323	4.026912797689302
4	1.5569995345188885	2.661828629526467
5	0.9188490826304728	1.9635673776194147
6	0.6178047693220033	1.5842879711721776
7	0.43513107989890953	1.3018164718024587
8	0.3204021185183233	1.095511608224773
9	0.26779883571083696	1.0301081227720201
>10	1.5660668171538046	12.310125607136028
>50	0.11061076093669181	3.2454528793380892
>100	0.08024828871501258	7.277999152680444
>500	0.014612304889547957	4.406193287554664
>1k	0.015295919203526276	12.614103282028207
>5k	7.263425320109768E-4	1.8807352471779286
>10k+	2.5635618776858005E-4	1.7529083603299644
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29095	0.524445524359003	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17088	0.3080159862604105	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15454	0.2785626785854626	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12464	0.22466709110192862	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	11520	0.20765122669241157	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	10371	0.1869401798634549	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	9825	0.17709837693167912	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7304	0.1316566458126193	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7278	0.1311879885301538	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	6215	0.11202711578935225	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	6182	0.11143228154622295	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	6108	0.11009841081920572	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	5925	0.10679978456185231	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	5832	0.10512343351303335	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	5832	0.10512343351303335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8025280094827392E-5
2	0.0	0.0	0.0	0.0	1.8025280094827392E-5
3	0.0	0.0	0.0	0.0	1.8025280094827392E-5
4	0.0	0.0	0.0	0.0	1.8025280094827392E-5
5	0.0	0.0	0.0	0.0	1.8025280094827392E-5
6	1.8025280094827392E-5	0.0	0.0	0.0	9.012640047413696E-5
7	1.8025280094827392E-5	0.0	0.0	0.0	9.012640047413696E-5
8	1.8025280094827392E-5	0.0	0.0	0.0	1.0815168056896436E-4
9	1.8025280094827392E-5	0.0	0.0	0.0	1.4420224075861914E-4
10	3.6050560189654784E-5	0.0	0.0	1.8025280094827392E-5	1.4420224075861914E-4
11	3.6050560189654784E-5	0.0	0.0	3.6050560189654784E-5	1.4420224075861914E-4
12	3.6050560189654784E-5	0.0	0.0	5.407584028448218E-5	1.4420224075861914E-4
13	3.6050560189654784E-5	0.0	0.0	7.210112037930957E-5	1.6222752085344653E-4
14	5.407584028448218E-5	0.0	0.0	1.4420224075861914E-4	2.1630336113792872E-4
15	5.407584028448218E-5	0.0	0.0	2.1630336113792872E-4	2.1630336113792872E-4
16	7.210112037930957E-5	0.0	0.0	5.227331227499945E-4	3.0642976161206567E-4
17	7.210112037930957E-5	0.0	0.0	7.931123241724053E-4	3.0642976161206567E-4
18	7.210112037930957E-5	0.0	0.0	0.0010274409654051615	3.6050560189654785E-4
19	7.210112037930957E-5	0.0	0.0	0.0015681993682499832	3.7853088199137525E-4
20	7.210112037930957E-5	0.0	0.0	0.0020008060905258407	3.7853088199137525E-4
21	7.210112037930957E-5	0.0	0.0	0.003352702097637895	3.9655616208620264E-4
22	7.210112037930957E-5	0.0	0.0	0.005263381787689599	3.9655616208620264E-4
23	7.210112037930957E-5	0.0	0.0	0.008327679403810255	4.686572824655122E-4
24	7.210112037930957E-5	0.0	0.0	0.013122403909034342	4.686572824655122E-4
25	7.210112037930957E-5	0.0	0.0	0.015952372883922244	4.686572824655122E-4
26	7.210112037930957E-5	0.0	0.0	0.02274790347967217	4.686572824655122E-4
27	7.210112037930957E-5	0.0	0.0	0.05002015226314602	4.686572824655122E-4
28	7.210112037930957E-5	0.0	0.0	0.09007232463385248	4.686572824655122E-4
29	7.210112037930957E-5	0.0	0.0	0.13682990119983474	4.686572824655122E-4
30	9.012640047413696E-5	0.0	0.0	0.21354549328342012	4.686572824655122E-4
31	9.012640047413696E-5	0.0	0.0	0.43188571107206436	4.686572824655122E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2405	0.0	18.76923	5
CAAGACG	2485	0.0	18.090544	4
GACGGAC	2875	0.0	15.507827	7
CGCAAGA	2870	0.0	15.470385	2
GCGCAAG	2880	0.0	15.352431	1
ACCGTCG	2005	0.0	15.132169	8
TCTTATA	4665	0.0	15.109325	37
CTTATAC	2805	0.0	15.103387	37
AGACGGA	3005	0.0	15.021631	6
ACGGACC	3000	0.0	14.985	8
TACCGTC	1995	0.0	14.744361	7
ATACCGT	2085	0.0	14.729016	6
CGGACCA	3065	0.0	14.606852	9
CGTATTA	90	8.28105E-4	14.388889	28
CGTCGTA	2045	0.0	14.3838625	10
AGGTTAT	1475	0.0	14.2983055	1
GGTTATC	1490	0.0	14.278523	2
TTATCTA	1555	0.0	14.276526	4
CCGTCGT	2105	0.0	14.237531	9
GCAAGAC	3410	0.0	13.725805	3
>>END_MODULE