FastQCFastQC Report
Thu 9 Feb 2017
SRR2727163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852284
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59400.6969507816643279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58080.6814629865162317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.5562699757357876No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT32260.3785123268769565No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.218706440576146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18590.21811978166902113No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG13310.1561686010766364No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA12570.14748604925118858No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11750.13786484317434095No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11400.13375823082446694No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.12495834721759415No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG10550.12378502940334443No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.11897442636492062No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG10110.1186224310206457No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9540.11193451947942236No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA9320.109353220288073No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.1068892528781486No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9020.10583326684532386No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT8640.10137465915117495No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8600.10090533202547507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAC405.9383547E-527.7500023
TATGCGA450.003825009320.55555517
CAAGACG6400.019.656254
AAGACGG6600.018.7803025
GTTACGG500.007033424518.523
GTTATCT2050.018.0487823
ATTCCGT3400.017.95588329
ATCGCAC953.6041747E-617.52631617
TCCGTGG3700.017.531
CGAGACT2650.017.45283120
GCGCAAG7250.017.3517251
GCAAGAC8000.017.343753
CCCTCGA752.0664762E-417.26666626
CGCAAGA7500.017.2666662
GTAACAC752.0664762E-417.2666663
GTGTATA2050.017.1463439
ATAGACT650.001579481117.0769234
TTATCTA2200.016.8181824
CGTGGGT3200.016.76562533
CTCGATT3650.016.72602725
Produced by FastQC (version 0.11.5)