##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727163.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852284
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.057452680092553	31.0	30.0	31.0	27.0	33.0
2	30.242659723754056	31.0	30.0	31.0	27.0	34.0
3	30.027933177203842	31.0	30.0	31.0	26.0	34.0
4	33.87327463615414	35.0	33.0	37.0	28.0	37.0
5	33.429530532076164	35.0	33.0	37.0	28.0	37.0
6	33.83908180841128	35.0	33.0	37.0	29.0	37.0
7	33.74683438853715	35.0	33.0	37.0	29.0	37.0
8	33.75344603442045	35.0	33.0	37.0	29.0	37.0
9	35.1737366887094	37.0	34.0	39.0	29.0	39.0
10	34.82353534737247	37.0	33.0	39.0	28.0	39.0
11	35.1056830821651	37.0	34.0	39.0	29.0	39.0
12	34.882352596082995	37.0	33.0	39.0	27.0	39.0
13	35.10271693473068	37.0	34.0	39.0	29.0	39.0
14	35.787841846145184	38.0	34.0	40.0	27.0	41.0
15	35.82842456270445	38.0	34.0	40.0	28.0	41.0
16	35.68188772756499	38.0	34.0	40.0	27.0	41.0
17	35.58873098638482	38.0	33.0	40.0	27.0	41.0
18	35.56968217167048	38.0	33.0	40.0	27.0	41.0
19	35.55332729465765	38.0	34.0	40.0	27.0	41.0
20	35.3855064743677	38.0	33.0	40.0	27.0	41.0
21	35.19765242571725	38.0	33.0	40.0	27.0	41.0
22	35.12889248184877	37.0	33.0	40.0	27.0	41.0
23	34.82280202373857	37.0	33.0	40.0	26.0	41.0
24	34.62230664895739	37.0	32.0	39.0	25.0	41.0
25	34.548279681420745	37.0	32.0	39.0	25.0	41.0
26	34.177910180174685	37.0	32.0	39.0	24.0	40.0
27	34.243469313045885	37.0	32.0	39.0	25.0	40.0
28	34.051313881288394	37.0	32.0	39.0	24.0	40.0
29	33.89543860966532	37.0	32.0	39.0	24.0	40.0
30	33.94819097859399	37.0	32.0	39.0	24.0	40.0
31	33.89681373814362	37.0	32.0	39.0	24.0	40.0
32	33.68975013023828	36.0	31.0	39.0	23.0	40.0
33	33.492249062519065	36.0	31.0	39.0	22.0	40.0
34	33.519644860163986	36.0	31.0	39.0	23.0	40.0
35	33.10043717821759	36.0	31.0	39.0	21.0	40.0
36	32.96130280516823	36.0	30.0	39.0	20.0	40.0
37	32.77427125230557	36.0	30.0	39.0	19.0	40.0
38	32.515463155473995	35.0	30.0	39.0	18.0	40.0
39	32.40547869020186	35.0	30.0	39.0	17.0	40.0
40	32.163492450873186	35.0	30.0	39.0	16.0	40.0
41	31.868972079729293	35.0	29.0	39.0	15.0	40.0
42	31.601250287462864	35.0	29.0	39.0	15.0	40.0
43	31.159899751725952	35.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	7.0
12	10.0
13	16.0
14	51.0
15	91.0
16	169.0
17	339.0
18	605.0
19	1171.0
20	1920.0
21	3349.0
22	5182.0
23	7694.0
24	10908.0
25	14695.0
26	19253.0
27	24800.0
28	30780.0
29	36831.0
30	43762.0
31	50480.0
32	57201.0
33	65856.0
34	74622.0
35	85293.0
36	94848.0
37	95102.0
38	83439.0
39	43807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.89841883691352	26.02700508281277	14.445184938353883	20.62939114191983
2	28.368008785803795	23.16105898972643	27.255703497895066	21.215228726574708
3	27.053188843155567	23.64352727494591	28.67753002520287	20.625753856695656
4	24.65117261382356	17.007945708238097	29.84603723641415	28.494844441524187
5	15.299477638909096	39.974703267924774	31.764763858056703	12.961055235109423
6	34.58365990679163	36.14827921209362	14.122170544090936	15.14589033702381
7	27.435221123475273	32.74014295704249	21.893641086773894	17.930994832708347
8	27.472884625312687	34.62754199304457	20.09353689615199	17.806036485490754
9	26.8282638181639	13.483650989576246	20.99804759915709	38.690037593102765
10	18.3156084122194	28.062946154098867	32.25438938194311	21.367056051738622
11	36.44172599743747	21.793205081874117	20.467238619990518	21.29783030069789
12	20.30062749036706	27.86406878458354	29.515513608140008	22.319790116909388
13	31.06124249663258	19.121912414171803	26.344035556223044	23.472809532972576
14	22.532864631977134	21.78757315636572	26.589024315838383	29.090537895818763
15	26.23421300880927	28.305470946304283	22.892721205607522	22.567594839278925
16	24.405714527082523	26.152198093593213	26.719497256782947	22.722590122541312
17	23.177954766251624	28.27672465985517	26.29768950256018	22.24763107133303
18	23.308897034321895	24.153451197018835	29.11154028469383	23.42611148396544
19	23.744549938752808	26.444236897559968	28.43875985000305	21.37245331368417
20	24.00725579736332	24.613509111986147	28.481585950223163	22.89764914042737
21	24.370280329092182	26.146331504521967	27.743569045060095	21.739819121325755
22	24.2900253905975	26.174725795626806	27.590333738519085	21.944915075256606
23	23.453449789037457	25.186088205340003	28.58460325431429	22.77585875130825
24	23.504137118613045	26.48412970324446	28.175701996048264	21.83603118209423
25	24.042220668227962	25.58724556603198	27.27905252239864	23.091481243341423
26	23.424351507244065	26.311886648112605	27.95054230749375	22.31321953714959
27	24.009485101210394	25.358800587597564	27.898681660103907	22.733032651088134
28	23.598119875534447	25.59592811785743	28.998080452055884	21.80787155455224
29	23.404405104401818	25.788234907612956	28.586715226379937	22.22064476160529
30	23.355243087984757	26.162171295014335	28.512209545175082	21.97037607182582
31	24.21375973267127	26.27175917886526	27.289729714508308	22.224751373955158
32	23.0768147706633	25.999666777740753	27.57942188284656	23.344096568749386
33	22.75086708186473	25.797152123001254	28.557382281023695	22.89459851411032
34	24.015821017407344	25.910142628513498	28.072567360175714	22.00146899390344
35	23.9902426890567	26.06314327149166	28.45319165911832	21.493422380333314
36	22.775272092401124	26.505718751026652	27.86348212567642	22.855527030895807
37	23.49087862731202	25.957544668209188	28.139094480243678	22.412482224235113
38	22.99679449573147	25.014431809115273	29.13571063166738	22.85306306348588
39	23.816591652547743	24.5865228022584	28.109174875980308	23.48771066921355
40	23.414495637604364	25.30799592624055	28.813400228092984	22.4641082080621
41	22.419170135776337	25.193128112225505	29.02365878040653	23.36404297159163
42	23.45661774713593	25.523417076936795	28.41494149837378	22.60502367755349
43	22.942000553806007	24.671353680228655	28.99409117148744	23.392554594477897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	538.0
2	445.0
3	586.5
4	728.0
5	728.0
6	1525.0
7	2322.0
8	2517.0
9	2712.0
10	3836.5
11	4961.0
12	4961.0
13	9045.0
14	13129.0
15	15462.5
16	17796.0
17	14427.5
18	11059.0
19	11059.0
20	12578.0
21	14097.0
22	11203.0
23	8309.0
24	8506.5
25	8704.0
26	8704.0
27	9861.5
28	11019.0
29	11927.0
30	12835.0
31	14524.0
32	16213.0
33	16213.0
34	19426.0
35	22639.0
36	26718.5
37	30798.0
38	34385.0
39	37972.0
40	37972.0
41	41547.5
42	45123.0
43	48781.0
44	52439.0
45	57641.5
46	62844.0
47	62844.0
48	71727.0
49	80610.0
50	77971.5
51	75333.0
52	69936.0
53	64539.0
54	64539.0
55	60799.5
56	57060.0
57	55673.0
58	54286.0
59	50246.5
60	46207.0
61	46207.0
62	38130.5
63	30054.0
64	26566.0
65	23078.0
66	19579.5
67	16081.0
68	16081.0
69	13087.0
70	10093.0
71	8709.5
72	7326.0
73	5622.5
74	3919.0
75	3919.0
76	3128.5
77	2338.0
78	1987.0
79	1636.0
80	1358.5
81	1081.0
82	1081.0
83	894.0
84	707.0
85	670.5
86	634.0
87	459.5
88	285.0
89	285.0
90	213.5
91	142.0
92	97.5
93	53.0
94	44.0
95	35.0
96	35.0
97	20.5
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	852284.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.46621982274665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10351305593925	55.65959633531277
2	6.071562590447386	7.58535126884903
3	1.8773279484452225	3.518087409208958
4	0.8767022738917	2.190571078400831
5	0.519133165443478	1.62141432149353
6	0.3057035890823134	1.145768855773104
7	0.19722298474358144	0.8623842023363538
8	0.15380773474099968	0.7686230215015994
9	0.10974332835770692	0.6169725785945149
>10	0.6165538806961146	7.278613318430486
>50	0.0832384953041181	3.66645562180148
>100	0.07853894136367992	9.406433736946395
>500	0.004321520689774411	1.8668738131506928
>1k	0.002254706446838823	2.4340015803545247
>5k	3.7578440780647044E-4	1.37885285784574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5940	0.6969507816643279	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5808	0.6814629865162317	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4741	0.5562699757357876	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3226	0.3785123268769565	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1864	0.218706440576146	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1859	0.21811978166902113	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	1331	0.1561686010766364	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA	1257	0.14748604925118858	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	1175	0.13786484317434095	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	1140	0.13375823082446694	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1065	0.12495834721759415	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG	1055	0.12378502940334443	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.11897442636492062	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1011	0.1186224310206457	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	954	0.11193451947942236	No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA	932	0.109353220288073	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	911	0.1068892528781486	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	902	0.10583326684532386	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	864	0.10137465915117495	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	860	0.10090533202547507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.3466356284994203E-4	0.0
11	0.0	0.0	0.0	2.3466356284994203E-4	0.0
12	0.0	0.0	0.0	2.3466356284994203E-4	0.0
13	0.0	0.0	0.0	2.3466356284994203E-4	0.0
14	1.1733178142497101E-4	0.0	0.0	3.5199534427491307E-4	0.0
15	2.3466356284994203E-4	0.0	0.0	5.86658907124855E-4	0.0
16	2.3466356284994203E-4	0.0	0.0	9.386542513997681E-4	0.0
17	2.3466356284994203E-4	0.0	0.0	0.0014079813770996523	0.0
18	2.3466356284994203E-4	0.0	0.0	0.0015253131585246233	0.0
19	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.0022293038470744494	0.0
20	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.0030506263170492465	0.0
21	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.00469327125699884	0.0
22	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.008330556481172943	0.0
23	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.01149851457964716	0.0
24	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.016778444743770857	0.0
25	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.01959440749797016	0.0
26	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.026282319039193508	0.0
27	2.3466356284994203E-4	2.3466356284994203E-4	0.0	0.05279930164123696	0.0
28	2.3466356284994203E-4	3.5199534427491307E-4	0.0	0.09034547169722769	0.0
29	2.3466356284994203E-4	3.5199534427491307E-4	0.0	0.13540087576441656	0.0
30	2.3466356284994203E-4	3.5199534427491307E-4	0.0	0.20943722984357327	0.0
31	2.3466356284994203E-4	3.5199534427491307E-4	0.0	0.39341346311792785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATAC	40	5.9383547E-5	27.750002	3
TATGCGA	45	0.0038250093	20.555555	17
CAAGACG	640	0.0	19.65625	4
AAGACGG	660	0.0	18.780302	5
GTTACGG	50	0.0070334245	18.5	23
GTTATCT	205	0.0	18.048782	3
ATTCCGT	340	0.0	17.955883	29
ATCGCAC	95	3.6041747E-6	17.526316	17
TCCGTGG	370	0.0	17.5	31
CGAGACT	265	0.0	17.452831	20
GCGCAAG	725	0.0	17.351725	1
GCAAGAC	800	0.0	17.34375	3
CCCTCGA	75	2.0664762E-4	17.266666	26
CGCAAGA	750	0.0	17.266666	2
GTAACAC	75	2.0664762E-4	17.266666	3
GTGTATA	205	0.0	17.146343	9
ATAGACT	65	0.0015794811	17.076923	4
TTATCTA	220	0.0	16.818182	4
CGTGGGT	320	0.0	16.765625	33
CTCGATT	365	0.0	16.726027	25
>>END_MODULE