FastQCFastQC Report
Thu 9 Feb 2017
SRR2727162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4359155
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197910.4540100088205168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127260.2919373135389772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT113610.2606239053211001No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91860.21072891420470252No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT75900.17411631382687698No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT44790.10274927136107802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG17950.018.0362135
CAAGACG18900.017.2275144
CTTATAC26700.015.10486937
TATTCCG1601.0988515E-815.0312515
GCGCAAG21450.014.6620041
CGCAAGA21450.014.6620042
TCGTTAC7200.014.64583323
TATCTAG15600.014.230771
ACGGACC22500.013.9777788
TCGTTTA12650.013.8932830
TACCGTC13100.013.8396957
TCTAGAT10050.013.8059712
TTCGGGC12300.013.68699235
GCAAGAC25350.013.6469433
CGAACGT8300.013.5963864
CGCTTCG12400.013.57661232
CGGACCA23350.013.548189
TCTTATA39950.013.52190237
AGACGGA23450.013.4904046
GACGGAC22950.013.3812637
Produced by FastQC (version 0.11.5)