##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4359155
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94190525457342	31.0	31.0	33.0	28.0	34.0
2	31.11627459909088	31.0	31.0	34.0	28.0	34.0
3	31.151162323890755	31.0	31.0	34.0	28.0	34.0
4	35.01171534391413	35.0	35.0	37.0	32.0	37.0
5	34.852603314174424	35.0	35.0	37.0	32.0	37.0
6	34.875516929313136	35.0	35.0	37.0	32.0	37.0
7	34.695851374865086	35.0	35.0	37.0	31.0	37.0
8	34.62264154406072	35.0	35.0	37.0	30.0	37.0
9	36.20833716626273	38.0	35.0	39.0	32.0	39.0
10	35.966165460966636	37.0	35.0	39.0	30.0	39.0
11	36.09364383693629	38.0	35.0	39.0	31.0	39.0
12	35.93679187824246	37.0	35.0	39.0	30.0	39.0
13	36.060393356051804	38.0	35.0	39.0	30.0	39.0
14	36.97159013616171	39.0	36.0	40.0	31.0	41.0
15	36.894623155175715	38.0	36.0	40.0	31.0	41.0
16	36.870739168485635	38.0	36.0	40.0	31.0	41.0
17	36.73339993645558	38.0	36.0	40.0	30.0	41.0
18	36.742817128549	38.0	36.0	40.0	31.0	41.0
19	36.75450838522604	39.0	35.0	40.0	30.0	41.0
20	36.592908946802766	38.0	35.0	40.0	30.0	41.0
21	36.45829639001137	38.0	35.0	40.0	30.0	41.0
22	36.371619499650734	38.0	35.0	40.0	30.0	41.0
23	36.14829731909051	38.0	34.0	40.0	29.0	41.0
24	36.010843844735966	38.0	34.0	40.0	29.0	41.0
25	35.863696977969354	38.0	34.0	40.0	27.0	41.0
26	35.568803816335965	38.0	34.0	40.0	27.0	41.0
27	35.70426240865489	38.0	34.0	40.0	27.0	41.0
28	35.56484135113342	38.0	34.0	40.0	27.0	41.0
29	35.44460933368967	38.0	34.0	40.0	27.0	41.0
30	35.60629273333937	38.0	34.0	40.0	27.0	41.0
31	35.55979083102115	38.0	34.0	40.0	27.0	41.0
32	35.43151390579138	38.0	34.0	40.0	27.0	41.0
33	35.3131994618223	38.0	34.0	40.0	26.0	41.0
34	35.370232992403345	38.0	34.0	40.0	26.0	41.0
35	35.05821334639397	38.0	33.0	40.0	25.0	41.0
36	34.90355768491829	38.0	33.0	40.0	25.0	41.0
37	34.74291210108381	38.0	33.0	40.0	24.0	41.0
38	34.57871169985926	38.0	33.0	40.0	24.0	41.0
39	34.51308338427975	38.0	33.0	40.0	24.0	41.0
40	34.379206291127524	37.0	33.0	40.0	24.0	41.0
41	34.17628118293568	37.0	32.0	40.0	23.0	41.0
42	34.01262262984454	37.0	32.0	40.0	23.0	41.0
43	33.61198512096954	37.0	31.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	9.0
12	11.0
13	19.0
14	31.0
15	96.0
16	245.0
17	504.0
18	895.0
19	1765.0
20	3643.0
21	6602.0
22	11597.0
23	18257.0
24	28695.0
25	41586.0
26	58559.0
27	78653.0
28	103421.0
29	131529.0
30	161920.0
31	195421.0
32	230667.0
33	271073.0
34	319933.0
35	376961.0
36	451275.0
37	553982.0
38	722343.0
39	589460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.01330533096437	21.10645297081659	13.376514485032075	25.50372721318696
2	19.749355092902178	22.989570226339737	33.121350353451525	24.139724327306553
3	20.952822278629686	24.246534018634343	29.947868336868037	24.852775365867927
4	15.776979712811311	16.406092465168136	34.19378296940577	33.623144852614786
5	14.7911235090287	37.52419907069145	32.929960049596765	14.754717370683078
6	34.77896518935436	35.296817846578065	15.180946766059018	14.74327019800856
7	28.169427331673226	31.625808212830236	21.4136684747388	18.79109598075774
8	27.339679364463986	33.302417555696	20.268285940738515	19.0896171391015
9	27.807384688087485	13.482452447779444	19.478889830712603	39.231273033420464
10	17.254720238211306	26.3618522397116	32.400224355408334	23.98320316666877
11	36.91630602720022	21.648140522647164	20.677424867892974	20.758128582259637
12	21.603842946626123	25.811011537786566	28.757201797137288	23.82794371845002
13	30.32317043096655	18.91212861208193	25.07967713926208	25.68502381768944
14	22.924534686194917	21.17873762231442	24.72442480251333	31.172302888977338
15	26.01816177676637	27.673643171669738	21.793902717384448	24.514292334179448
16	24.824031262939723	25.952346268944325	24.668886515849977	24.554735952265975
17	24.191408656035403	26.21723705626435	25.022280694308876	24.56907359339138
18	24.42650009004039	24.310743710650343	26.892344961351455	24.370411237957814
19	24.87647261911999	25.536164692469065	26.29986774959826	23.287494938812685
20	24.54071947430179	24.225406070671955	26.03841799614834	25.195456458877924
21	25.372256779123475	25.558118488560282	25.83438762787742	23.23523710443882
22	25.158224472403486	24.721717855868857	25.324541109458142	24.795516562269523
23	23.56940737367678	24.68694047355508	26.33127291871934	25.4123792340488
24	23.95746423332045	25.76744804899115	25.9657663010377	24.309321416650704
25	24.84148877477401	24.778059050435232	25.242575682672445	25.13787649211831
26	24.227195408284405	25.79554982559693	25.930140130369306	24.04711463574936
27	25.469890380131012	25.217869059485153	25.015490387471885	24.296750172911953
28	23.773345981044493	25.888847723928148	26.06378988588385	24.274016409143513
29	23.993962132569273	25.75684966467125	26.42165740837387	23.82753079438561
30	24.393603806242265	25.88091040580112	26.004076478124777	23.721409309831838
31	24.81903029371518	25.688854835398146	25.22066776703283	24.271447103853845
32	23.731388308055116	25.687363720721102	25.209725279325923	25.37152269189786
33	23.3953598805273	24.977891357384628	26.22200862323088	25.404740138857186
34	24.63229685569795	25.278224793566643	26.364834469065677	23.724643881669728
35	24.969701696773804	25.185202177945037	26.29261863824526	23.552477487035905
36	23.68874242829172	25.808098129109887	26.14174077315443	24.361418669443964
37	23.926242586005774	25.301004437786684	26.67402283240674	24.098730143800807
38	23.823860358257505	24.395851948370726	27.216490351914537	24.56379734145723
39	23.927963102940822	24.038167947687107	26.99110263342322	25.04276631594885
40	24.26064225750174	24.354421900574767	27.448691317468636	23.936244524454857
41	22.777097854974187	24.57217052387447	28.164999868093705	24.485731753057646
42	23.94076374893758	24.78436761253041	27.696078712502768	23.57878992602924
43	22.74617443059492	23.938125622970507	27.805297127539625	25.510402818894946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1273.0
1	1352.0
2	1431.0
3	2256.5
4	3082.0
5	3082.0
6	4922.0
7	6762.0
8	6881.5
9	7001.0
10	9502.0
11	12003.0
12	12003.0
13	19976.0
14	27949.0
15	37445.0
16	46941.0
17	40690.5
18	34440.0
19	34440.0
20	36342.5
21	38245.0
22	33624.0
23	29003.0
24	33558.0
25	38113.0
26	38113.0
27	44149.0
28	50185.0
29	55906.0
30	61627.0
31	69996.5
32	78366.0
33	78366.0
34	94254.0
35	110142.0
36	134342.5
37	158543.0
38	181716.0
39	204889.0
40	204889.0
41	222485.0
42	240081.0
43	254620.5
44	269160.0
45	298313.5
46	327467.0
47	327467.0
48	366122.0
49	404777.0
50	406302.0
51	407827.0
52	381610.5
53	355394.0
54	355394.0
55	338842.5
56	322291.0
57	313240.0
58	304189.0
59	282205.5
60	260222.0
61	260222.0
62	215392.5
63	170563.0
64	149548.0
65	128533.0
66	109196.0
67	89859.0
68	89859.0
69	75879.0
70	61899.0
71	54112.5
72	46326.0
73	34339.0
74	22352.0
75	22352.0
76	17608.5
77	12865.0
78	11206.0
79	9547.0
80	7820.0
81	6093.0
82	6093.0
83	5119.0
84	4145.0
85	3761.0
86	3377.0
87	2291.5
88	1206.0
89	1206.0
90	872.0
91	538.0
92	390.5
93	243.0
94	206.0
95	169.0
96	169.0
97	91.5
98	14.0
99	18.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4359155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.691431512607124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.75524193141409	38.17269279437247
2	9.260811068997127	8.648010515585467
3	3.1400970304582927	4.398468763217531
4	1.6031827918055341	2.9941959810311354
5	0.9409237691752812	2.19665388635159
6	0.6409051883703	1.7954868425319854
7	0.42712413449184233	1.3960126091105214
8	0.3354127624289588	1.2528721620324872
9	0.25547101608729467	1.0735476705986462
>10	1.4463506713553902	12.128809007033277
>50	0.09306255585216627	3.000648161598823
>100	0.07588535384680098	7.673190318451804
>500	0.01385866052155261	4.486658094607302
>1k	0.011424702106152622	9.37601368471058
>5k	9.934523570567495E-5	0.38908335804836136
>10k+	1.4901785355851243E-4	1.0176561507180497
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19791	0.4540100088205168	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12726	0.2919373135389772	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11361	0.2606239053211001	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9186	0.21072891420470252	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7590	0.17411631382687698	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	4479	0.10274927136107802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.882067740192766E-5
2	0.0	0.0	0.0	0.0	6.882067740192766E-5
3	0.0	0.0	0.0	0.0	6.882067740192766E-5
4	0.0	0.0	0.0	0.0	9.176090320257022E-5
5	0.0	0.0	0.0	0.0	9.176090320257022E-5
6	2.2940225800642555E-5	0.0	0.0	0.0	1.1470112900321277E-4
7	2.2940225800642555E-5	0.0	0.0	0.0	1.1470112900321277E-4
8	2.2940225800642555E-5	0.0	0.0	0.0	1.3764135480385533E-4
9	2.2940225800642555E-5	0.0	0.0	0.0	1.8352180640514044E-4
10	2.2940225800642555E-5	0.0	0.0	2.2940225800642555E-5	1.8352180640514044E-4
11	2.2940225800642555E-5	0.0	0.0	4.588045160128511E-5	1.8352180640514044E-4
12	2.2940225800642555E-5	0.0	0.0	4.588045160128511E-5	2.06462032205783E-4
13	2.2940225800642555E-5	0.0	0.0	6.882067740192766E-5	2.2940225800642555E-4
14	2.2940225800642555E-5	0.0	0.0	1.1470112900321277E-4	3.441033870096383E-4
15	2.2940225800642555E-5	0.0	0.0	1.8352180640514044E-4	3.670436128102809E-4
16	4.588045160128511E-5	0.0	0.0	5.276251934147788E-4	4.3586429021220854E-4
17	4.588045160128511E-5	0.0	0.0	0.0012617124190353406	4.3586429021220854E-4
18	4.588045160128511E-5	0.0	0.0	0.0015599353544436937	4.8174474181349365E-4
19	4.588045160128511E-5	0.0	0.0	0.0019269789672539746	4.8174474181349365E-4
20	4.588045160128511E-5	0.0	0.0	0.0023857834832668257	5.046849676141362E-4
21	4.588045160128511E-5	0.0	0.0	0.0034410338700963834	5.505654192154213E-4
22	4.588045160128511E-5	0.0	0.0	0.005551534643755499	5.735056450160639E-4
23	4.588045160128511E-5	0.0	0.0	0.008304361739832605	6.652665482186341E-4
24	4.588045160128511E-5	0.0	0.0	0.012525363287150836	6.652665482186341E-4
25	4.588045160128511E-5	0.0	0.0	0.015232309931626657	6.882067740192766E-4
26	4.588045160128511E-5	0.0	0.0	0.021426170897800148	6.882067740192766E-4
27	4.588045160128511E-5	0.0	0.0	0.05494184079253892	7.340872256205618E-4
28	4.588045160128511E-5	0.0	0.0	0.09823004687835142	7.570274514212043E-4
29	4.588045160128511E-5	0.0	0.0	0.14876736431716697	7.799676772218469E-4
30	4.588045160128511E-5	0.0	0.0	0.2256630012009208	7.799676772218469E-4
31	4.588045160128511E-5	0.0	0.0	0.4747020924926964	7.799676772218469E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1795	0.0	18.036213	5
CAAGACG	1890	0.0	17.227514	4
CTTATAC	2670	0.0	15.104869	37
TATTCCG	160	1.0988515E-8	15.031251	5
GCGCAAG	2145	0.0	14.662004	1
CGCAAGA	2145	0.0	14.662004	2
TCGTTAC	720	0.0	14.645833	23
TATCTAG	1560	0.0	14.23077	1
ACGGACC	2250	0.0	13.977778	8
TCGTTTA	1265	0.0	13.89328	30
TACCGTC	1310	0.0	13.839695	7
TCTAGAT	1005	0.0	13.805971	2
TTCGGGC	1230	0.0	13.686992	35
GCAAGAC	2535	0.0	13.646943	3
CGAACGT	830	0.0	13.596386	4
CGCTTCG	1240	0.0	13.576612	32
CGGACCA	2335	0.0	13.54818	9
TCTTATA	3995	0.0	13.521902	37
AGACGGA	2345	0.0	13.490404	6
GACGGAC	2295	0.0	13.381263	7
>>END_MODULE