FastQCFastQC Report
Thu 9 Feb 2017
SRR2727161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6532456
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT202780.3104192358892276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142430.21803438094340016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108830.16659890246486161No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC89470.1369622696272275No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87520.13397717489409802No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG79280.12136323612436119No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75760.11597475742660954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG19950.018.1754385
CAAGACG22150.016.9548534
GACGGAC22750.015.1252757
ACGGACC22950.014.9128548
TTAGAGT12300.014.8902444
GCGCAAG23450.014.7526651
TATACCG2650.014.6603775
GGTTATC10450.014.51674652
TACCGTC16450.014.50767
ACCGTCG15950.014.4984328
CCGTCGT15600.014.4679499
CGAGCCG16250.014.34461715
AGACGGA24750.014.2020216
CGCAAGA24750.014.2020212
GTATTAG17700.014.1101691
CGGACCA24550.014.091659
AGGTTAT11300.014.0796461
TAGAGTG12550.014.00398355
ACGCGTA1356.5777804E-613.7037036
CGAACGA9050.013.69613416
Produced by FastQC (version 0.11.5)