##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6532456
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.455321245179455	31.0	31.0	34.0	30.0	34.0
2	31.665721284613323	31.0	31.0	34.0	30.0	34.0
3	31.839850126812948	31.0	31.0	34.0	30.0	34.0
4	35.55597221014577	37.0	35.0	37.0	33.0	37.0
5	35.358176771492985	37.0	35.0	37.0	33.0	37.0
6	35.38701630749598	37.0	35.0	37.0	33.0	37.0
7	35.27693244317298	37.0	35.0	37.0	32.0	37.0
8	35.251238125446235	37.0	35.0	37.0	32.0	37.0
9	36.761938541951146	39.0	35.0	39.0	32.0	39.0
10	36.546893388948966	38.0	35.0	39.0	32.0	39.0
11	36.626977510449365	39.0	35.0	39.0	32.0	39.0
12	36.45772692537079	38.0	35.0	39.0	32.0	39.0
13	36.45382349303233	38.0	35.0	39.0	32.0	39.0
14	37.41439207550728	39.0	36.0	41.0	32.0	41.0
15	37.35062065477364	39.0	36.0	41.0	32.0	41.0
16	37.26553749462683	39.0	36.0	41.0	31.0	41.0
17	37.584436848866645	39.0	36.0	41.0	32.0	41.0
18	37.621063348915015	39.0	37.0	41.0	32.0	41.0
19	37.69990199704368	40.0	37.0	41.0	32.0	41.0
20	37.67888386848683	40.0	37.0	41.0	32.0	41.0
21	37.62868957709015	40.0	37.0	41.0	32.0	41.0
22	37.57446984717539	39.0	37.0	41.0	32.0	41.0
23	37.44506354118573	39.0	36.0	41.0	32.0	41.0
24	37.318027247332395	39.0	36.0	41.0	31.0	41.0
25	37.23931213620115	39.0	36.0	41.0	31.0	41.0
26	36.94044353302954	39.0	36.0	41.0	30.0	41.0
27	36.746994545389974	39.0	36.0	41.0	30.0	41.0
28	36.52895220419395	39.0	36.0	40.0	30.0	41.0
29	36.33969872893135	39.0	35.0	40.0	29.0	41.0
30	36.19168946564661	39.0	35.0	40.0	29.0	41.0
31	36.03232949445048	39.0	35.0	40.0	27.0	41.0
32	35.85655731932982	38.0	35.0	40.0	27.0	41.0
33	35.68673451455318	38.0	35.0	40.0	27.0	41.0
34	35.560954256714474	38.0	34.0	40.0	26.0	41.0
35	35.386551551208306	38.0	34.0	40.0	25.0	41.0
36	35.27469316287779	38.0	34.0	40.0	25.0	41.0
37	35.19233593000856	38.0	34.0	40.0	25.0	41.0
38	35.06758928035642	38.0	34.0	40.0	24.0	41.0
39	34.922568479603996	38.0	34.0	40.0	24.0	41.0
40	34.80635124063599	38.0	33.0	40.0	24.0	41.0
41	34.57164166126798	38.0	33.0	40.0	23.0	41.0
42	34.38381597977851	38.0	33.0	40.0	22.0	41.0
43	34.090696822144686	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	22.0
11	61.0
12	72.0
13	110.0
14	152.0
15	278.0
16	506.0
17	821.0
18	1394.0
19	2686.0
20	5042.0
21	9137.0
22	15525.0
23	24979.0
24	37026.0
25	51967.0
26	69680.0
27	91524.0
28	118523.0
29	147937.0
30	182619.0
31	223001.0
32	273009.0
33	331141.0
34	405424.0
35	498791.0
36	633809.0
37	834031.0
38	1274974.0
39	1298210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09444533572059	21.256568739230698	13.028178069626492	25.620807855422218
2	18.99129515759463	23.372143647044847	33.74318939155503	23.89337180380549
3	19.04109266101448	25.67380476806885	30.374808494691734	24.91029407622493
4	14.396331180799383	16.751968937869616	34.9115554701019	33.9401444112291
5	12.831682295295982	39.56839510285259	34.341050287977446	13.258872313873985
6	33.58274437669385	38.1732689818347	14.9412257809314	13.302760860540047
7	28.238460389170626	32.306654648726294	21.332436069986542	18.122448892116534
8	27.19352415079413	35.820202998688394	19.256172563580986	17.73010028693649
9	27.6751347425838	14.123371056766398	18.387739618912093	39.81375458173771
10	16.179397151699145	27.909533565935995	33.29779794919399	22.61327133317086
11	36.92654033949865	22.609352439572497	20.63543941206799	19.828667808860864
12	21.236162937798586	26.41788938188026	29.680689774259484	22.66525790606167
13	29.953848904608005	20.323076037557698	24.838758959876653	24.884316097957644
14	22.38348027143237	21.286572768343177	24.78762964496049	31.54231731526397
15	24.976639720190995	28.7436761916192	21.74514455206434	24.534539536125465
16	24.540846505510334	26.871470699534754	24.243576994624995	24.34410580032992
17	23.777044958282154	27.331190596614814	24.852322005689746	24.039442439413293
18	24.313826224011308	25.716055339676224	25.94958159687566	24.020536839436808
19	24.770790649030015	26.18506730087428	25.608209224830595	23.43593282526511
20	24.40262896527738	25.26648476468881	25.70483444511528	24.62605182491853
21	24.82675734823166	26.05118197504889	25.600922532046138	23.521138144673305
22	24.830324153733297	25.46218451375715	25.150601856330912	24.556889476178636
23	23.638429405418115	25.47640581122935	25.747712652025516	25.13745213132702
24	23.98917038247177	26.104944296601463	25.541144096492957	24.36474122443381
25	24.574784736399298	25.23461313784586	25.144279578767925	25.04632254698692
26	24.342192278065095	25.96648182551861	25.56912744609378	24.122198450322514
27	25.027371022476082	25.720387554083796	24.92650543685254	24.32573598658759
28	24.15575397675851	26.125304173499213	25.482284151626892	24.23665769811538
29	24.290022007036864	25.99553674758774	25.795749714961723	23.91869153041368
30	24.438006164909492	26.21280571962521	25.53387271188662	23.815315403578687
31	24.895889080615316	25.55072701599521	25.25849389571089	24.294890007678582
32	23.930233896715112	26.003711314703075	25.14663703819819	24.919417750383623
33	23.928106059956622	25.47038969722873	25.580210567051658	25.02129367576299
34	24.60814125651975	25.659154841609343	25.853874867278098	23.87882903459281
35	24.823619171717347	25.684872580848612	25.775956240654356	23.715552006779685
36	24.214292449884088	25.869489208959077	25.71248853417459	24.203729806982246
37	24.447145147246303	25.69814477127745	25.873010089926364	23.981699991549885
38	24.291108887683286	25.120980531671393	26.11267492655136	24.475235654093957
39	24.50280568288558	25.065549618703898	25.85575777318668	24.575886925223838
40	24.570513754704205	25.228887879229493	26.221301758481037	23.979296607585262
41	23.568011786072496	25.68204056789667	26.50110463813304	24.248843007897793
42	24.56096145155819	25.572112540826915	26.389309013332813	23.477616994282087
43	23.96158504550203	24.79457955782634	26.22759341968779	25.01624197698385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1575.0
1	1446.0
2	1317.0
3	1389.0
4	1461.0
5	1461.0
6	2040.0
7	2619.0
8	3172.0
9	3725.0
10	5229.5
11	6734.0
12	6734.0
13	13271.5
14	19809.0
15	30175.0
16	40541.0
17	36262.0
18	31983.0
19	31983.0
20	39729.5
21	47476.0
22	46668.0
23	45860.0
24	56091.0
25	66322.0
26	66322.0
27	80353.5
28	94385.0
29	108407.5
30	122430.0
31	140461.5
32	158493.0
33	158493.0
34	183553.0
35	208613.0
36	242356.5
37	276100.0
38	311076.0
39	346052.0
40	346052.0
41	369572.0
42	393092.0
43	414448.5
44	435805.0
45	471154.0
46	506503.0
47	506503.0
48	559866.0
49	613229.0
50	606147.0
51	599065.0
52	560738.0
53	522411.0
54	522411.0
55	497472.0
56	472533.0
57	445404.5
58	418276.0
59	384683.0
60	351090.0
61	351090.0
62	298805.5
63	246521.0
64	211959.5
65	177398.0
66	148735.0
67	120072.0
68	120072.0
69	96564.0
70	73056.0
71	62992.0
72	52928.0
73	41607.0
74	30286.0
75	30286.0
76	23180.0
77	16074.0
78	13671.5
79	11269.0
80	8446.0
81	5623.0
82	5623.0
83	4690.5
84	3758.0
85	3202.0
86	2646.0
87	2575.5
88	2505.0
89	2505.0
90	1753.5
91	1002.0
92	878.5
93	755.0
94	736.0
95	717.0
96	717.0
97	424.5
98	132.0
99	173.5
100	215.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6532456.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.681375734258616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02275876720802	41.063448801070656
2	9.814254226900578	9.94799812050168
3	3.3266143333156895	5.057921728492281
4	1.626486990806333	3.297303932317576
5	0.9402878786829996	2.382754163895104
6	0.6141230254509976	1.8674759879965006
7	0.44512896702572263	1.579182389962314
8	0.3046681539413363	1.2352800947331048
9	0.2437581244908752	1.111859738604021
>10	1.4803770790684645	13.985318873795466
>50	0.11057876276921494	3.845070790019474
>100	0.059166718431399305	5.74283361402776
>500	0.005959018627653268	2.1404110627551236
>1k	0.005444723267163323	5.042285805432321
>5k	3.024846258251607E-4	1.0047962763708815
>10k+	9.074538774754821E-5	0.6960586200256855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20278	0.3104192358892276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14243	0.21803438094340016	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10883	0.16659890246486161	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8947	0.1369622696272275	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	8752	0.13397717489409802	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	7928	0.12136323612436119	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7576	0.11597475742660954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5308178118612663E-5
2	0.0	0.0	0.0	0.0	1.5308178118612663E-5
3	0.0	0.0	0.0	0.0	1.5308178118612663E-5
4	0.0	0.0	0.0	0.0	1.5308178118612663E-5
5	0.0	0.0	0.0	0.0	1.5308178118612663E-5
6	0.0	0.0	0.0	0.0	3.0616356237225326E-5
7	0.0	0.0	0.0	0.0	3.0616356237225326E-5
8	0.0	0.0	0.0	1.5308178118612663E-5	4.592453435583799E-5
9	0.0	0.0	0.0	1.5308178118612663E-5	7.654089059306331E-5
10	0.0	0.0	0.0	1.5308178118612663E-5	7.654089059306331E-5
11	0.0	0.0	0.0	1.5308178118612663E-5	7.654089059306331E-5
12	0.0	0.0	0.0	1.5308178118612663E-5	7.654089059306331E-5
13	0.0	0.0	0.0	1.5308178118612663E-5	9.184906871167598E-5
14	0.0	0.0	0.0	1.5308178118612663E-5	1.6838995930473929E-4
15	0.0	1.5308178118612663E-5	0.0	3.0616356237225326E-5	1.6838995930473929E-4
16	0.0	1.5308178118612663E-5	0.0	9.184906871167598E-5	2.2962267177918994E-4
17	0.0	1.5308178118612663E-5	0.0	1.5308178118612662E-4	2.2962267177918994E-4
18	0.0	1.5308178118612663E-5	0.0	1.6838995930473929E-4	2.449308498978026E-4
19	0.0	1.5308178118612663E-5	0.0	2.449308498978026E-4	2.449308498978026E-4
20	0.0	1.5308178118612663E-5	0.0	3.3677991860947857E-4	2.449308498978026E-4
21	0.0	1.5308178118612663E-5	0.0	5.051698779142179E-4	2.6023902801641525E-4
22	0.0	1.5308178118612663E-5	0.0	8.266416184050838E-4	2.6023902801641525E-4
23	0.0	1.5308178118612663E-5	0.0	0.0013471196744379143	3.520880967280912E-4
24	0.0	1.5308178118612663E-5	0.0	0.0021431449366057726	3.520880967280912E-4
25	0.0	1.5308178118612663E-5	0.0	0.002678931170757216	3.520880967280912E-4
26	0.0	1.5308178118612663E-5	0.0	0.003597421857873976	3.8270445296531656E-4
27	0.0	1.5308178118612663E-5	0.0	0.008143950759101936	3.8270445296531656E-4
28	0.0	1.5308178118612663E-5	0.0	0.016226668805729424	3.8270445296531656E-4
29	0.0	1.5308178118612663E-5	0.0	0.0293763938096177	3.8270445296531656E-4
30	0.0	1.5308178118612663E-5	0.0	0.05145078665665716	3.8270445296531656E-4
31	0.0	1.5308178118612663E-5	0.0	0.13018074672068208	3.8270445296531656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1995	0.0	18.175438	5
CAAGACG	2215	0.0	16.954853	4
GACGGAC	2275	0.0	15.125275	7
ACGGACC	2295	0.0	14.912854	8
TTAGAGT	1230	0.0	14.890244	4
GCGCAAG	2345	0.0	14.752665	1
TATACCG	265	0.0	14.660377	5
GGTTATC	1045	0.0	14.5167465	2
TACCGTC	1645	0.0	14.5076	7
ACCGTCG	1595	0.0	14.498432	8
CCGTCGT	1560	0.0	14.467949	9
CGAGCCG	1625	0.0	14.344617	15
AGACGGA	2475	0.0	14.202021	6
CGCAAGA	2475	0.0	14.202021	2
GTATTAG	1770	0.0	14.110169	1
CGGACCA	2455	0.0	14.09165	9
AGGTTAT	1130	0.0	14.079646	1
TAGAGTG	1255	0.0	14.0039835	5
ACGCGTA	135	6.5777804E-6	13.703703	6
CGAACGA	905	0.0	13.696134	16
>>END_MODULE