##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3802584
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.040771485915894	31.0	31.0	33.0	30.0	34.0
2	31.1358962747437	31.0	31.0	34.0	28.0	34.0
3	31.203550533005977	31.0	31.0	34.0	28.0	34.0
4	35.02031487009886	35.0	35.0	37.0	32.0	37.0
5	34.86173480980302	35.0	35.0	37.0	32.0	37.0
6	34.947858614037195	35.0	35.0	37.0	32.0	37.0
7	34.7538400203651	35.0	35.0	37.0	32.0	37.0
8	34.73202143594987	35.0	35.0	37.0	31.0	37.0
9	36.31201414617008	38.0	35.0	39.0	32.0	39.0
10	36.10161984587323	38.0	35.0	39.0	31.0	39.0
11	36.23119647061051	38.0	35.0	39.0	32.0	39.0
12	36.117231861281695	38.0	35.0	39.0	31.0	39.0
13	36.21173470461139	38.0	35.0	39.0	32.0	39.0
14	37.12546836572184	39.0	36.0	40.0	31.0	41.0
15	37.028331787016405	39.0	36.0	40.0	31.0	41.0
16	36.954461755479954	39.0	36.0	40.0	31.0	41.0
17	36.943705911559086	39.0	36.0	40.0	31.0	41.0
18	36.94212987799875	38.0	36.0	40.0	31.0	41.0
19	36.950162573660435	39.0	36.0	40.0	31.0	41.0
20	36.91293788644774	39.0	36.0	40.0	31.0	41.0
21	36.771494068244124	39.0	35.0	40.0	30.0	41.0
22	36.76660712820545	39.0	35.0	40.0	30.0	41.0
23	36.54913737605796	38.0	35.0	40.0	30.0	41.0
24	36.42010801076321	38.0	35.0	40.0	30.0	41.0
25	36.35009220046158	38.0	35.0	40.0	30.0	41.0
26	35.978347092398224	38.0	34.0	40.0	29.0	41.0
27	36.095784603311856	38.0	35.0	40.0	29.0	41.0
28	35.94285333341749	38.0	34.0	40.0	29.0	41.0
29	35.84285238669284	38.0	34.0	40.0	28.0	41.0
30	35.88905202357134	38.0	34.0	40.0	29.0	41.0
31	35.772149149104926	38.0	34.0	40.0	28.0	41.0
32	35.48107760407134	38.0	34.0	40.0	27.0	41.0
33	35.412116602815345	38.0	34.0	40.0	27.0	41.0
34	35.37883817951162	38.0	34.0	40.0	27.0	41.0
35	35.08464586186656	38.0	33.0	40.0	25.0	41.0
36	34.91604445818948	38.0	33.0	40.0	25.0	41.0
37	34.657779026051756	38.0	33.0	40.0	24.0	41.0
38	34.47252105410426	38.0	33.0	40.0	24.0	41.0
39	34.356083915568995	38.0	33.0	40.0	23.0	41.0
40	34.171038693688295	38.0	33.0	40.0	22.0	41.0
41	33.979681974152314	37.0	33.0	40.0	21.0	41.0
42	33.772083141358614	37.0	33.0	40.0	20.0	41.0
43	33.29082013704365	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	12.0
15	60.0
16	162.0
17	343.0
18	723.0
19	1436.0
20	2847.0
21	5141.0
22	8687.0
23	14173.0
24	22275.0
25	33089.0
26	47575.0
27	65344.0
28	88106.0
29	112661.0
30	138843.0
31	166800.0
32	195081.0
33	229682.0
34	272422.0
35	321228.0
36	389879.0
37	498296.0
38	631493.0
39	556215.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.34665585296735	19.376955249377794	12.711145894475967	24.565243003178892
2	15.815061547621301	26.688220431159444	31.886028027257257	25.610689993961998
3	22.639499876925797	27.270193110789926	29.024657969422897	21.06564904286138
4	16.536860198223103	18.221767093113524	30.69594254854068	34.5454301601227
5	13.616924701729141	38.65013895814005	33.0746408231876	14.658295516943214
6	33.24657653848015	31.333693088699683	17.441955259896954	17.97777511292321
7	24.620757884638444	31.57781655842448	23.704880681136828	20.096544875800245
8	28.365185358166972	31.280334635605684	23.547908474868667	16.806571531358678
9	28.04516612913745	13.877063596754208	20.74620836778359	37.33156190632475
10	18.518538972446105	28.00945357157133	32.753227805092536	20.718779650890028
11	32.28012846001561	23.315855744409593	20.33456723112494	24.069448564449857
12	21.302119821679153	28.912786673483083	29.485476191978925	20.299617312858835
13	31.342634377044664	19.06848080147605	25.64122186386941	23.94766295760988
14	22.64865154852595	22.766545065145174	27.022414232006447	27.56238915432243
15	28.454124879292607	24.303605127460695	24.758821895847667	22.48344809739903
16	21.608332649587755	25.43359988891764	30.609396136942667	22.348671324551937
17	24.28390799519485	27.86021294993089	27.85090349088935	20.00497556398491
18	24.463733082556494	21.841831764926166	31.410667062187187	22.283768090330156
19	22.980662623100503	29.474036602478737	29.032073979167848	18.513226795252912
20	22.698801656978517	24.485455153653408	30.693733524361328	22.122009665006743
21	21.1765736141529	28.03354245428898	29.360429644683723	21.4294542868744
22	22.46940501511604	27.97852723306047	25.42223919313814	24.129828558685357
23	19.513941046404234	26.65221859661746	28.574648186601532	25.259192170376778
24	20.633074772312725	31.478463066167638	27.11272124429072	20.775740917228916
25	22.908211889599283	26.327728723415444	24.76218802793048	26.00187135905479
26	21.937030187893285	26.93952322946712	28.559132421532308	22.564314161107287
27	23.800631360148785	26.130731102850064	26.179750401306055	23.888887135695096
28	21.25089149904381	27.258727223382838	29.522792921865765	21.967588355707594
29	22.58661478615594	25.227424298845207	29.593692078860062	22.592268836138793
30	20.8004083538983	29.182787283594525	30.292190783951124	19.724613578556056
31	24.68871167606028	26.267559112435123	25.237654184628138	23.80607502687646
32	18.221767093113524	28.536384732066406	29.037517645895527	24.20433052892454
33	21.186093456449616	27.473949293427836	29.409896007556966	21.930061242565582
34	20.110298681107373	27.241160221575644	28.97895220723592	23.669588890081062
35	19.879666037620733	29.454155384864606	30.61278856693238	20.053390010582277
36	22.754053559369105	27.376647037908956	27.445573851885978	22.423725550835957
37	20.560624038811504	28.010952552264463	29.106681141034624	22.321742267889412
38	21.93881844556228	27.159768199729445	29.114281236127855	21.78713211858042
39	22.146650803769226	23.900274129381494	28.756340425352867	25.196734641496416
40	21.234376413512496	26.689114559993943	29.608077033932716	22.46843199256085
41	19.946751998114966	26.047314142172795	29.785088245256386	24.220845614455854
42	21.911836793085964	27.20839302958199	29.13652926536271	21.743240911969334
43	20.92179949213482	24.114207601988543	30.56908144566958	24.39491146020706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1155.0
1	1320.0
2	1485.0
3	5453.0
4	9421.0
5	9421.0
6	14460.0
7	19499.0
8	19761.5
9	20024.0
10	24521.5
11	29019.0
12	29019.0
13	43260.5
14	57502.0
15	76455.0
16	95408.0
17	84525.0
18	73642.0
19	73642.0
20	72068.5
21	70495.0
22	57470.5
23	44446.0
24	44849.5
25	45253.0
26	45253.0
27	46456.5
28	47660.0
29	48647.5
30	49635.0
31	52755.5
32	55876.0
33	55876.0
34	64257.0
35	72638.0
36	86080.5
37	99523.0
38	113361.5
39	127200.0
40	127200.0
41	139945.0
42	152690.0
43	172433.5
44	192177.0
45	235333.5
46	278490.0
47	278490.0
48	457070.5
49	635651.0
50	622584.0
51	609517.0
52	424494.0
53	239471.0
54	239471.0
55	209521.5
56	179572.0
57	171465.0
58	163358.0
59	151484.5
60	139611.0
61	139611.0
62	116604.5
63	93598.0
64	80436.0
65	67274.0
66	56811.0
67	46348.0
68	46348.0
69	38929.0
70	31510.0
71	27066.0
72	22622.0
73	16992.0
74	11362.0
75	11362.0
76	8883.0
77	6404.0
78	5698.5
79	4993.0
80	4066.5
81	3140.0
82	3140.0
83	2633.5
84	2127.0
85	1893.0
86	1659.0
87	1129.5
88	600.0
89	600.0
90	429.5
91	259.0
92	197.5
93	136.0
94	129.0
95	122.0
96	122.0
97	64.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3802584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.98939166926814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53759215962727	31.735423076596632
2	8.185874246057722	6.219527657777235
3	2.826470491891179	3.221276836742489
4	1.4235837498072212	2.163243225817278
5	0.842918910368696	1.6010988315714556
6	0.5741495188442781	1.3086954568858276
7	0.41503392487291946	1.103682042962166
8	0.29835027983149043	0.9067316508123391
9	0.2328511984179308	0.7961287839621519
>10	1.3954247803809137	9.709668816640098
>50	0.120058936778262	3.1998167932617525
>100	0.11549000763978283	9.294668897721532
>500	0.01789771565107945	4.580675595586192
>1k	0.011415078486093896	8.573967032613947
>5k	0.0014797323963455051	4.296740683776705
>10k+	0.0014092689489004812	11.288654617272275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	45709	1.2020510263547104	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	45118	1.1865089633785868	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34772	0.9144308186222843	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	30579	0.8041636950031874	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	26255	0.6904515455805842	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	21963	0.5775809291786848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21460	0.5643530820094966	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20158	0.5301132072296102	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19399	0.5101530958947916	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	18401	0.48390778481159125	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	17480	0.45968741255945955	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16572	0.43580891309698877	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	15797	0.4154280352518182	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	14095	0.370668997713134	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	13544	0.3561788510128902	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	13366	0.3514978235852252	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	12566	0.3304594980676298	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	12392	0.32588366226755283	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	12000	0.3155748827639311	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	10086	0.265240688963084	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9984	0.26255830245959066	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	9953	0.2617430673457838	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	9837	0.25869251014573247	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	9546	0.25103981923870716	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	9501	0.24985641342834242	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	8792	0.23121119743837346	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	8731	0.22960702511765682	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7854	0.20654376076899286	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	7697	0.20241498938616478	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	7586	0.19949592172059843	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7503	0.1973131954481479	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	7255	0.19079131453769332	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	7252	0.19071242081700235	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	7187	0.1890030568686977	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7075	0.18605769129623437	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	6341	0.16675502763384056	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	6165	0.16212659601996957	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	6112	0.16073280695442888	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	5850	0.15384275534741637	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	5253	0.13814290492991083	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	5040	0.13254145076085103	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4997	0.1314106407642803	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	4995	0.1313580449504863	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	4978	0.1309109805332374	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4902	0.12891233960906584	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	4887	0.12851787100561093	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	4811	0.12651923008143937	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4734	0.1244942912503708	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	4707	0.12378424776415195	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4595	0.1208388821916886	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	4511	0.11862985801234108	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4507	0.1185246663847531	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	4492	0.11813019778129819	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	4427	0.11642083383299356	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4350	0.11439589500192501	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	4345	0.11426440546744004	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	4237	0.11142423152256464	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	4019	0.1056912878190199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.259581379398851E-5
2	0.0	0.0	0.0	0.0	7.889372069098277E-5
3	0.0	0.0	0.0	0.0	7.889372069098277E-5
4	0.0	0.0	0.0	2.6297906896994255E-5	7.889372069098277E-5
5	0.0	0.0	0.0	2.6297906896994255E-5	7.889372069098277E-5
6	0.0	0.0	0.0	2.6297906896994255E-5	1.3148953448497127E-4
7	0.0	0.0	0.0	2.6297906896994255E-5	1.3148953448497127E-4
8	2.6297906896994255E-5	0.0	0.0	2.6297906896994255E-5	1.3148953448497127E-4
9	2.6297906896994255E-5	0.0	0.0	2.6297906896994255E-5	1.5778744138196554E-4
10	5.259581379398851E-5	0.0	0.0	1.3148953448497127E-4	1.5778744138196554E-4
11	5.259581379398851E-5	0.0	0.0	2.366811620729483E-4	1.5778744138196554E-4
12	5.259581379398851E-5	2.6297906896994255E-5	0.0	4.207665103519081E-4	1.5778744138196554E-4
13	5.259581379398851E-5	5.259581379398851E-5	0.0	6.574476724248564E-4	1.5778744138196554E-4
14	5.259581379398851E-5	5.259581379398851E-5	0.0	0.0010782141827767645	2.366811620729483E-4
15	7.889372069098277E-5	5.259581379398851E-5	0.0	0.0017093639483046264	2.6297906896994254E-4
16	7.889372069098277E-5	5.259581379398851E-5	0.0	0.0040498776621371154	2.6297906896994254E-4
17	7.889372069098277E-5	5.259581379398851E-5	0.0	0.0068637537001155005	2.6297906896994254E-4
18	7.889372069098277E-5	5.259581379398851E-5	0.0	0.00951984229671192	3.1557488276393107E-4
19	7.889372069098277E-5	5.259581379398851E-5	0.0	0.014884615303698749	3.1557488276393107E-4
20	7.889372069098277E-5	5.259581379398851E-5	0.0	0.019276365755496787	3.1557488276393107E-4
21	7.889372069098277E-5	5.259581379398851E-5	0.0	0.029585145259118536	3.1557488276393107E-4
22	7.889372069098277E-5	5.259581379398851E-5	0.0	0.044364568935229304	3.1557488276393107E-4
23	7.889372069098277E-5	5.259581379398851E-5	0.0	0.0645087656183269	3.418727896609253E-4
24	7.889372069098277E-5	5.259581379398851E-5	0.0	0.09230565320844983	3.418727896609253E-4
25	7.889372069098277E-5	5.259581379398851E-5	0.0	0.11092457129152176	3.418727896609253E-4
26	7.889372069098277E-5	5.259581379398851E-5	0.0	0.14626895816108204	3.418727896609253E-4
27	7.889372069098277E-5	5.259581379398851E-5	0.0	0.2169051360864086	3.418727896609253E-4
28	7.889372069098277E-5	5.259581379398851E-5	0.0	0.3486050538265558	3.418727896609253E-4
29	7.889372069098277E-5	5.259581379398851E-5	0.0	0.45979260418704754	3.6817069655791955E-4
30	7.889372069098277E-5	5.259581379398851E-5	0.0	0.6462184661798398	3.6817069655791955E-4
31	7.889372069098277E-5	5.259581379398851E-5	0.0	1.0150992062239783	3.6817069655791955E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	2110	0.0	19.464455	2
AAAAAGT	2070	0.0	19.304348	1
GGGCTGT	3665	0.0	16.758528	37
TGGGCTG	3520	0.0	16.713068	36
AAAGTAC	2510	0.0	16.288845	3
AGTACTC	2515	0.0	16.25646	5
TACACCG	115	1.243965E-6	16.086956	5
CTATACG	70	0.0025939704	15.857142	4
TATACGG	70	0.0025939704	15.857142	2
AAGTACT	2645	0.0	15.807183	4
ATACTAG	110	1.453543E-5	15.136364	1
CCTATAC	185	3.0559022E-10	15.000001	3
TATCACG	160	1.786666E-7	13.875	2
GCGAGTA	80	0.006302149	13.875	2
GCGGGTA	360	0.0	13.361111	23
CGGGTAA	365	0.0	13.178082	24
CGAGCCG	765	0.0	13.058824	15
CTTATAC	4120	0.0	12.976941	37
TAGGACT	260	2.3646862E-11	12.807693	4
TCTAGAT	610	0.0	12.737705	2
>>END_MODULE