FastQCFastQC Report
Thu 9 Feb 2017
SRR2727159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207529
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG74470.616713967117974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA69310.5739820741365218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60710.5027622525007681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57610.4770899912134616No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG56070.46433667431589637No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT55490.45953347704278735No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.3997419523671895No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG42870.35502252947962326No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG42010.3479005473160479No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40880.3385425940080942No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC35210.2915871999761496No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT34200.28322301162125296No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT24430.20231398169319328No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT23040.1908028709869494No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT22600.18715906615907363No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22340.18500590876078338No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21080.17457137675368462No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG20960.17357761180062756No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC20290.16802909081272582No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC20130.16670407087531644No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC19590.16223212858655983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19410.1607414811569743No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC18760.15535858766124871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC18750.15527577391516062No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT18250.15113508661075636No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG18130.1501413216576993No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT18090.14981006667334698No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16330.13523484736184388No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG15370.1272847277373877No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT14590.120825255542517No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA14450.1196658630972838No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT14200.11759551944508166No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA14090.1166845682381127No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA14080.11660175449202462No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA13530.11204699845717991No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT13450.11138448848847522No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG13310.11022509604324202No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.1086516348675684No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC12830.10625003623101392No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.10542189877013307No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12600.10434532007098794No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT12160.10070151524311217No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12140.10053588775093601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC250.00549645329.5999983
CAAGACG7200.020.81254
AAGACGG7350.020.1360535
TACCGTC3200.019.0781257
GGTCGCG3000.018.57
AGGTTAT3000.018.51
CGTCGTA3300.018.510
ATACCGT3350.018.223886
GTCGCGT3050.018.1967228
TTATCTA3050.018.1967224
TCGCGTA2950.018.1864419
GCAAGAC8550.017.9590643
CGCAAGA8700.017.862072
GGGTTAG1258.578354E-917.761
ACCGTCG3550.017.7183118
GTCGTAG3450.017.69565211
GCGCAAG8500.017.4117661
CCTAGAC852.7232994E-517.4117643
GGGTCGC3200.017.343756
CCGTCGT3550.017.1971849
Produced by FastQC (version 0.11.5)