##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1207529
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.03333915790014	31.0	30.0	31.0	27.0	33.0
2	30.19400113786087	31.0	30.0	31.0	27.0	34.0
3	29.985025618432353	31.0	30.0	31.0	26.0	34.0
4	33.786063937180806	35.0	33.0	37.0	28.0	37.0
5	33.30251116122263	35.0	33.0	37.0	28.0	37.0
6	33.791055949794995	35.0	33.0	37.0	29.0	37.0
7	33.69972729433413	35.0	33.0	37.0	29.0	37.0
8	33.73313353136861	35.0	33.0	37.0	29.0	37.0
9	35.19227861194224	37.0	34.0	39.0	29.0	39.0
10	34.82702195972105	37.0	33.0	39.0	28.0	39.0
11	35.11530157867844	37.0	34.0	39.0	29.0	39.0
12	34.872697881375935	37.0	33.0	39.0	27.0	39.0
13	35.067639783392366	37.0	34.0	39.0	29.0	39.0
14	35.75648286707814	38.0	34.0	40.0	27.0	41.0
15	35.80360388860226	38.0	34.0	40.0	28.0	41.0
16	35.583518076998566	38.0	34.0	40.0	27.0	41.0
17	35.55328526271419	38.0	33.0	40.0	27.0	41.0
18	35.47980711022261	38.0	33.0	40.0	27.0	41.0
19	35.5363216949655	38.0	34.0	40.0	27.0	41.0
20	35.391352091751	38.0	33.0	40.0	27.0	41.0
21	35.13897885682248	37.0	33.0	40.0	27.0	41.0
22	35.125731969998235	37.0	33.0	40.0	27.0	41.0
23	34.81013209620639	37.0	33.0	40.0	26.0	41.0
24	34.591132800951364	37.0	32.0	39.0	25.0	41.0
25	34.561215507039584	37.0	32.0	39.0	25.0	40.0
26	34.13192229751832	37.0	32.0	39.0	24.0	40.0
27	34.27969266162552	37.0	32.0	39.0	25.0	40.0
28	34.07378290707718	37.0	32.0	39.0	24.0	40.0
29	33.960798457014285	37.0	32.0	39.0	24.0	40.0
30	33.99967122942803	37.0	32.0	39.0	24.0	40.0
31	34.00218628289672	37.0	32.0	39.0	24.0	40.0
32	33.74345957736833	36.0	31.0	39.0	24.0	40.0
33	33.54802244915029	36.0	31.0	39.0	23.0	40.0
34	33.51471890116097	36.0	31.0	39.0	23.0	40.0
35	33.13032978918105	36.0	31.0	39.0	21.0	40.0
36	33.04479561153397	36.0	31.0	39.0	21.0	40.0
37	32.85376500274528	36.0	30.0	39.0	19.0	40.0
38	32.641311305981056	35.0	30.0	39.0	18.0	40.0
39	32.53645916578401	35.0	30.0	39.0	18.0	40.0
40	32.32981650958279	35.0	30.0	39.0	16.0	40.0
41	32.059151374418335	35.0	30.0	39.0	15.0	40.0
42	31.805035738272124	35.0	29.0	39.0	15.0	40.0
43	31.305908181087162	35.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	7.0
11	8.0
12	14.0
13	25.0
14	58.0
15	138.0
16	269.0
17	503.0
18	899.0
19	1612.0
20	2786.0
21	4659.0
22	7034.0
23	10538.0
24	14973.0
25	19703.0
26	26470.0
27	33849.0
28	42415.0
29	51032.0
30	61217.0
31	71270.0
32	82621.0
33	93969.0
34	109384.0
35	126052.0
36	141316.0
37	138465.0
38	110352.0
39	55887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.59755831951034	25.005610631297472	13.687207512200535	21.709623536991657
2	27.513956186559497	25.45462676258707	25.527337231652407	21.50407981920103
3	28.40561179068991	23.485233066866304	28.610575812257927	19.49857933018586
4	26.642838391458923	17.88412535019863	28.110960482108503	27.362075776233947
5	15.834567948264597	39.34182947159033	32.04966505980394	12.773937520341129
6	34.5161068595454	35.19120451765548	14.121151541702105	16.171537081097018
7	25.59292571855417	33.0328298533617	22.298926154154476	19.075318273929653
8	26.91852535218616	33.84895932105979	21.971480602122185	17.26103472463187
9	27.243569305581893	13.899376329678212	19.795549423657736	39.06150494108216
10	18.863563525182418	27.779042987787456	32.35483371413854	21.002559772891583
11	36.19772278761007	21.745978771524328	19.467275734164563	22.58902270670104
12	20.587248836259832	28.805519370549277	28.630865180049508	21.976366613141384
13	32.400132833248726	18.706134593868967	25.563361211200725	23.330371361681582
14	23.202589751467666	22.225387547628255	26.004592850358044	28.567429850546034
15	27.650598867604835	26.695756375209207	22.897669538371336	22.75597521881462
16	24.293163973701667	26.59464079123566	26.36988428435259	22.74231095071009
17	23.793797084790512	29.718126852439987	25.33454683075934	21.15352923201016
18	23.139982559425075	23.52299613508247	29.51672382195376	23.820297483538695
19	24.25631185669247	29.202776910533824	25.752259366027648	20.788651866746058
20	24.446866286441153	24.50235149632017	27.04730072735313	24.00348148988554
21	23.338901177528655	27.82773747048725	25.92749325275004	22.905868099234056
22	24.508231272292424	28.235926424955426	24.967102239366508	22.288740063385642
23	23.380887746795313	24.6669024097972	27.467497675004076	24.48471216840341
24	23.07356593506243	28.630119856334712	26.707929995884154	21.5883842127187
25	25.469450423136834	26.714141026840764	24.343349103831045	23.473059446191353
26	23.264203178557203	27.511306146684678	26.448474529390186	22.776016145367937
27	24.338131837827497	25.97585648046548	26.31622097688751	23.36979070481951
28	23.39372387743897	26.054446725503073	28.552689003742355	21.999140393315606
29	23.41740860882016	26.146452797406937	26.658490189469568	23.777648404303335
30	22.44103454244163	27.702026203925538	27.35669288273822	22.50024637089461
31	25.470444188089893	27.121501843848055	24.455478916034316	22.952575052027736
32	22.167997621589212	27.636603344515947	26.46255286622516	23.73284616766968
33	23.953213546010073	25.724351133595963	28.220771509421304	22.101663810972656
34	24.50624374238631	26.268603072886865	26.979145014322636	22.24600817040419
35	23.226854179071477	28.04553762269892	28.135638978442756	20.591969219786854
36	23.522830507590296	27.67776177632173	25.733212204427385	23.066195511660588
37	22.41006220140469	27.459381927887446	27.154296087298942	22.976259783408928
38	23.21931812817746	25.54141556848738	28.36652370253634	22.87274260079882
39	23.562995174443017	24.28703575649115	26.99595620477852	25.154012864287317
40	23.493928510205553	25.95242019032255	27.90765273546225	22.64599856400964
41	22.72583101523856	24.981263389947568	27.993199335171248	24.299706259642626
42	23.65450436387035	26.313570937012692	27.555942755826152	22.475981943290805
43	23.36730629243687	24.280327843058014	28.851480999628166	23.50088486487695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	511.0
1	469.5
2	428.0
3	693.0
4	958.0
5	958.0
6	1888.5
7	2819.0
8	3158.0
9	3497.0
10	4815.0
11	6133.0
12	6133.0
13	10448.5
14	14764.0
15	18005.0
16	21246.0
17	18354.5
18	15463.0
19	15463.0
20	17471.0
21	19479.0
22	16145.5
23	12812.0
24	12473.0
25	12134.0
26	12134.0
27	12927.0
28	13720.0
29	14495.5
30	15271.0
31	16812.5
32	18354.0
33	18354.0
34	22527.0
35	26700.0
36	32458.5
37	38217.0
38	42047.0
39	45877.0
40	45877.0
41	50582.5
42	55288.0
43	61449.5
44	67611.0
45	78527.0
46	89443.0
47	89443.0
48	125667.0
49	161891.0
50	156316.0
51	150741.0
52	120063.0
53	89385.0
54	89385.0
55	80178.5
56	70972.0
57	69681.5
58	68391.0
59	63467.5
60	58544.0
61	58544.0
62	47832.0
63	37120.0
64	32967.5
65	28815.0
66	24622.0
67	20429.0
68	20429.0
69	17289.5
70	14150.0
71	12192.0
72	10234.0
73	7963.0
74	5692.0
75	5692.0
76	4494.5
77	3297.0
78	2900.5
79	2504.0
80	2063.5
81	1623.0
82	1623.0
83	1341.0
84	1059.0
85	1038.5
86	1018.0
87	795.5
88	573.0
89	573.0
90	385.5
91	198.0
92	146.0
93	94.0
94	76.0
95	58.0
96	58.0
97	32.5
98	7.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1207529.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.03496909456241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96754158571129	46.65355849322832
2	6.462447332402115	6.8547138909836685
3	2.0873580216396888	3.321089045008435
4	0.9666794647101805	2.0507126214101024
5	0.5764339339557781	1.5285577936200863
6	0.36810372614113285	1.1713421843692946
7	0.25083632710416337	0.9312167799033995
8	0.17207224457196005	0.7300676938324707
9	0.13622644810261914	0.6502288918485973
>10	0.7913268320201909	8.06608236266006
>50	0.10294547634310833	3.8997791522240366
>100	0.10183470984914925	10.997978679799672
>500	0.007941238171908203	2.79710251887424
>1k	0.007318395962346774	7.261846626899861
>5k	9.342633143421416E-4	3.085723265337748
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7447	0.616713967117974	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6931	0.5739820741365218	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6071	0.5027622525007681	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5761	0.4770899912134616	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	5607	0.46433667431589637	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	5549	0.45953347704278735	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4827	0.3997419523671895	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4287	0.35502252947962326	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	4201	0.3479005473160479	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4088	0.3385425940080942	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	3521	0.2915871999761496	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3420	0.28322301162125296	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	2443	0.20231398169319328	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	2304	0.1908028709869494	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	2260	0.18715906615907363	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	2234	0.18500590876078338	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2108	0.17457137675368462	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	2096	0.17357761180062756	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	2029	0.16802909081272582	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	2013	0.16670407087531644	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	1959	0.16223212858655983	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.1607414811569743	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1876	0.15535858766124871	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	1875	0.15527577391516062	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	1825	0.15113508661075636	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	1813	0.1501413216576993	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	1809	0.14981006667334698	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	1633	0.13523484736184388	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	1537	0.1272847277373877	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	1459	0.120825255542517	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA	1445	0.1196658630972838	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	1420	0.11759551944508166	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	1409	0.1166845682381127	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	1408	0.11660175449202462	No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA	1353	0.11204699845717991	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	1345	0.11138448848847522	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1331	0.11022509604324202	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1312	0.1086516348675684	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	1283	0.10625003623101392	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1273	0.10542189877013307	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	1260	0.10434532007098794	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	1216	0.10070151524311217	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	1214	0.10053588775093601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.281374608808568E-5
2	0.0	0.0	0.0	0.0	8.281374608808568E-5
3	0.0	0.0	0.0	0.0	8.281374608808568E-5
4	0.0	0.0	0.0	0.0	8.281374608808568E-5
5	0.0	0.0	0.0	0.0	8.281374608808568E-5
6	0.0	0.0	0.0	0.0	8.281374608808568E-5
7	0.0	0.0	0.0	0.0	8.281374608808568E-5
8	8.281374608808568E-5	0.0	0.0	0.0	1.6562749217617135E-4
9	8.281374608808568E-5	0.0	0.0	0.0	1.6562749217617135E-4
10	8.281374608808568E-5	0.0	0.0	0.0	1.6562749217617135E-4
11	8.281374608808568E-5	0.0	0.0	0.0	1.6562749217617135E-4
12	8.281374608808568E-5	0.0	0.0	0.0	1.6562749217617135E-4
13	8.281374608808568E-5	0.0	0.0	0.0	1.6562749217617135E-4
14	1.6562749217617135E-4	0.0	0.0	2.48441238264257E-4	2.48441238264257E-4
15	1.6562749217617135E-4	0.0	0.0	4.96882476528514E-4	3.312549843523427E-4
16	2.48441238264257E-4	0.0	0.0	8.281374608808566E-4	4.140687304404283E-4
17	2.48441238264257E-4	0.0	0.0	0.002235971144378313	4.140687304404283E-4
18	2.48441238264257E-4	0.0	0.0	0.0025672261287306556	4.140687304404283E-4
19	2.48441238264257E-4	0.0	0.0	0.0048860110191970545	4.140687304404283E-4
20	2.48441238264257E-4	0.0	0.0	0.006873540925311111	4.140687304404283E-4
21	2.48441238264257E-4	0.0	0.0	0.01167673819842008	4.140687304404283E-4
22	2.48441238264257E-4	0.0	0.0	0.018219024139378846	4.140687304404283E-4
23	2.48441238264257E-4	0.0	0.0	0.02525819255686613	4.140687304404283E-4
24	2.48441238264257E-4	0.0	0.0	0.039088088153576436	4.140687304404283E-4
25	2.48441238264257E-4	0.0	0.0	0.04778353149282543	4.140687304404283E-4
26	2.48441238264257E-4	0.0	0.0	0.0636009569956498	4.140687304404283E-4
27	2.48441238264257E-4	0.0	0.0	0.10774068366059945	4.140687304404283E-4
28	2.48441238264257E-4	0.0	0.0	0.1791261327885293	4.140687304404283E-4
29	2.48441238264257E-4	0.0	0.0	0.25506633795130385	4.140687304404283E-4
30	2.48441238264257E-4	0.0	0.0	0.377961937146023	4.140687304404283E-4
31	2.48441238264257E-4	0.0	0.0	0.6522410641897627	4.140687304404283E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	25	0.005496453	29.599998	3
CAAGACG	720	0.0	20.8125	4
AAGACGG	735	0.0	20.136053	5
TACCGTC	320	0.0	19.078125	7
GGTCGCG	300	0.0	18.5	7
AGGTTAT	300	0.0	18.5	1
CGTCGTA	330	0.0	18.5	10
ATACCGT	335	0.0	18.22388	6
GTCGCGT	305	0.0	18.196722	8
TTATCTA	305	0.0	18.196722	4
TCGCGTA	295	0.0	18.186441	9
GCAAGAC	855	0.0	17.959064	3
CGCAAGA	870	0.0	17.86207	2
GGGTTAG	125	8.578354E-9	17.76	1
ACCGTCG	355	0.0	17.718311	8
GTCGTAG	345	0.0	17.695652	11
GCGCAAG	850	0.0	17.411766	1
CCTAGAC	85	2.7232994E-5	17.411764	3
GGGTCGC	320	0.0	17.34375	6
CCGTCGT	355	0.0	17.197184	9
>>END_MODULE