##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727158.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2163896
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.866233867062004	31.0	31.0	31.0	30.0	34.0
2	31.119017272549144	31.0	31.0	34.0	28.0	34.0
3	31.224001061049144	31.0	31.0	34.0	28.0	34.0
4	34.97895277776751	35.0	35.0	37.0	32.0	37.0
5	34.70063857043037	35.0	35.0	37.0	32.0	37.0
6	34.90085521670173	35.0	35.0	37.0	32.0	37.0
7	34.83008102052964	35.0	35.0	37.0	32.0	37.0
8	34.808762066199115	35.0	35.0	37.0	32.0	37.0
9	36.27103335834994	38.0	35.0	39.0	32.0	39.0
10	35.91242508882127	37.0	35.0	39.0	30.0	39.0
11	36.1165222358191	37.0	35.0	39.0	32.0	39.0
12	35.878542221992184	37.0	35.0	39.0	30.0	39.0
13	35.945629549664126	37.0	35.0	39.0	30.0	39.0
14	36.75725034844558	38.0	36.0	40.0	31.0	41.0
15	36.71772488141759	38.0	36.0	40.0	31.0	41.0
16	36.60935830557476	38.0	36.0	40.0	30.0	41.0
17	36.88636006536358	38.0	36.0	40.0	31.0	41.0
18	36.90031544954101	38.0	36.0	40.0	31.0	41.0
19	36.977270626684465	39.0	36.0	40.0	31.0	41.0
20	36.93290435399853	39.0	36.0	40.0	31.0	41.0
21	36.86755648145752	39.0	36.0	40.0	31.0	41.0
22	36.79835260104922	39.0	36.0	40.0	31.0	41.0
23	36.64193981596158	39.0	36.0	40.0	30.0	41.0
24	36.51596241224162	38.0	35.0	40.0	30.0	41.0
25	36.441968560411404	38.0	35.0	40.0	30.0	41.0
26	36.10469449548407	38.0	35.0	40.0	29.0	41.0
27	35.87788969525337	38.0	34.0	40.0	27.0	41.0
28	35.65425094366827	38.0	34.0	40.0	27.0	41.0
29	35.44318534717011	38.0	34.0	40.0	27.0	41.0
30	35.28281858277847	38.0	34.0	40.0	26.0	41.0
31	35.10405721901607	38.0	34.0	40.0	25.0	41.0
32	34.92061587063334	38.0	34.0	40.0	25.0	41.0
33	34.73017048878504	38.0	33.0	40.0	25.0	41.0
34	34.595080817192695	38.0	33.0	40.0	24.0	41.0
35	34.41989310022293	38.0	33.0	40.0	24.0	41.0
36	34.30224373075231	38.0	33.0	40.0	24.0	41.0
37	34.19955071777941	38.0	33.0	40.0	23.0	41.0
38	34.07485295041906	37.0	33.0	40.0	23.0	41.0
39	33.89703433067024	37.0	32.0	40.0	22.0	41.0
40	33.78208194848551	37.0	32.0	40.0	21.0	40.0
41	33.53630534924044	37.0	31.0	40.0	20.0	40.0
42	33.32278861830698	37.0	31.0	40.0	19.0	40.0
43	33.02522856921035	37.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	21.0
12	26.0
13	34.0
14	56.0
15	144.0
16	236.0
17	399.0
18	766.0
19	1448.0
20	2584.0
21	4482.0
22	7382.0
23	11271.0
24	16454.0
25	22552.0
26	30085.0
27	38593.0
28	48991.0
29	60498.0
30	74571.0
31	89975.0
32	108282.0
33	130430.0
34	158185.0
35	195627.0
36	250531.0
37	332913.0
38	427341.0
39	150003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.14027661218469	25.574565505920805	15.613227253065768	22.671930628828743
2	21.981647916535728	26.001573088540297	30.372115850299647	21.644663144624328
3	22.267059045351534	26.231297622436568	29.368555605260145	22.133087726951757
4	17.815920913019852	19.159839474725217	32.48182907126775	30.54241054098718
5	13.190097860525645	40.432765715173005	33.781244569979336	12.59589185432202
6	33.494909182326694	39.598622114926044	13.894706584789656	13.011762117957609
7	27.82911008662154	32.657715527918164	21.253193314281276	18.25998107117902
8	27.05157734013095	37.00843293762732	18.737083482755178	17.202906239486556
9	27.548412677873614	13.711610909211903	17.935150302972048	40.80482610994244
10	15.840502501044412	28.94834132509141	33.66575842831633	21.545397745547845
11	37.69506482751482	22.462031446982664	20.113304890808063	19.72959883469446
12	20.72761352671293	27.06252980734749	29.87125998661673	22.33859667932285
13	30.45358926676698	20.67631716126838	24.78409313571447	24.08600043625017
14	22.206843582131487	21.08405394713979	25.24696196120331	31.462140509525412
15	25.07176869868053	29.283893495805714	21.547939457349152	24.09639834816461
16	24.41314185154924	27.27668057984302	24.236007645469098	24.074169923138637
17	23.57400725358335	27.947600069504265	24.933129873154716	23.545262803757666
18	24.112572877809285	26.08346242148421	26.171451862751262	23.632512837955243
19	24.67909733184959	26.671706958190228	25.906697918938804	22.74249779102138
20	24.366697844998093	25.444938204054168	26.066825762421114	24.12153818852662
21	24.715051000602617	26.262491358179872	25.805352937479437	23.217104703738073
22	24.672581307049875	25.977172655247756	25.525394935800982	23.824851101901384
23	23.75077175612876	25.742688188341766	26.204309264400877	24.302230791128594
24	24.004480806840995	26.38772843057152	25.73122737876497	23.876563383822514
25	24.400433292542708	25.766395427506684	25.592542340297314	24.24062893965329
26	24.2093427780263	26.261289821691985	25.75937106034671	23.769996339935005
27	24.822542303326962	26.136468665776913	25.27237907921638	23.768609951679746
28	24.145522705342586	26.378947971621557	25.870097268999988	23.60543205403587
29	24.32695471501403	26.274968852477198	26.02897736305257	23.369099069456205
30	24.428438335298924	26.399096814264638	25.619484485391165	23.55298036504527
31	24.880770610047804	25.935488581706327	25.647813018740273	23.535927789505596
32	24.021995511799087	26.374326677437367	25.51268637679445	24.0909914339691
33	24.029713073086693	25.915432164946928	25.78358664187188	24.271268120094497
34	24.359581051954436	26.088083715668404	26.18499225471095	23.36734297766621
35	24.569387807916833	26.20310772791299	25.939971237064995	23.287533227105182
36	24.167751130368558	26.187349114744883	25.904525910672234	23.740373844214325
37	24.247422242104058	26.165028263835232	26.092335306317864	23.49521418774285
38	24.354266563642614	25.417210438949006	26.3231227378765	23.90540025953188
39	24.347981603552114	25.644300835160283	26.03609415609623	23.971623405191377
40	24.354497628351822	25.732844831729434	26.414624362723533	23.498033177195207
41	23.630664320281568	25.836546673222742	26.56657251549982	23.96621649099587
42	24.27944781080052	25.967052020984372	26.363143145511614	23.390357022703494
43	23.871295108452532	25.397939642200917	26.48851885672879	24.242246392617762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	501.0
1	499.5
2	498.0
3	483.0
4	468.0
5	468.0
6	675.5
7	883.0
8	1116.5
9	1350.0
10	1975.5
11	2601.0
12	2601.0
13	5835.5
14	9070.0
15	13819.5
16	18569.0
17	15198.5
18	11828.0
19	11828.0
20	15322.0
21	18816.0
22	17746.5
23	16677.0
24	19804.0
25	22931.0
26	22931.0
27	28021.5
28	33112.0
29	38000.5
30	42889.0
31	49510.0
32	56131.0
33	56131.0
34	64772.0
35	73413.0
36	84732.0
37	96051.0
38	105945.0
39	115839.0
40	115839.0
41	124569.0
42	133299.0
43	140965.5
44	148632.0
45	160492.5
46	172353.0
47	172353.0
48	185648.5
49	198944.0
50	197065.5
51	195187.0
52	182980.5
53	170774.0
54	170774.0
55	161727.5
56	152681.0
57	143227.0
58	133773.0
59	121989.0
60	110205.0
61	110205.0
62	93218.0
63	76231.0
64	65037.5
65	53844.0
66	45174.5
67	36505.0
68	36505.0
69	29297.5
70	22090.0
71	18821.0
72	15552.0
73	12191.5
74	8831.0
75	8831.0
76	6882.0
77	4933.0
78	4141.0
79	3349.0
80	2555.0
81	1761.0
82	1761.0
83	1463.0
84	1165.0
85	948.0
86	731.0
87	721.0
88	711.0
89	711.0
90	490.5
91	270.0
92	240.5
93	211.0
94	191.5
95	172.0
96	172.0
97	107.5
98	43.0
99	32.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2163896.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.83134967988981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.77150076591452	52.41536306746308
2	8.320783558373055	10.289705556168844
3	2.604779975676951	4.8317118454576935
4	1.2934312085634765	3.1989838937428323
5	0.7293809592567763	2.25493045708296
6	0.4708387076753087	1.7467555666259658
7	0.33167728577682537	1.4355637966420542
8	0.2344922943435602	1.1599180039037056
9	0.1833936787304899	1.0205530810799663
>10	0.9522604182546733	10.361547262817737
>50	0.06225671261945026	2.661174694516786
>100	0.04064918837048503	5.137617444086463
>500	0.002912370677556088	1.2070158315316475
>1k	0.0014935234243876464	1.5337379930371775
>5k	1.4935234243876463E-4	0.7454215058431585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9566	0.4420730016599689	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6578	0.30398873143626126	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3738	0.1727439766051603	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2988	0.13808427022370762	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2551	0.11788921463878116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.621294184193695E-5	0.0
5	0.0	0.0	0.0	4.621294184193695E-5	0.0
6	0.0	0.0	0.0	4.621294184193695E-5	0.0
7	0.0	0.0	0.0	4.621294184193695E-5	0.0
8	0.0	0.0	0.0	4.621294184193695E-5	0.0
9	0.0	0.0	0.0	9.24258836838739E-5	2.3106470920968475E-4
10	0.0	0.0	0.0	1.3863882552581086E-4	2.3106470920968475E-4
11	0.0	0.0	0.0	1.848517673677478E-4	2.3106470920968475E-4
12	0.0	0.0	0.0	1.848517673677478E-4	2.3106470920968475E-4
13	0.0	0.0	0.0	1.848517673677478E-4	2.3106470920968475E-4
14	0.0	0.0	0.0	1.848517673677478E-4	3.2349059289355864E-4
15	0.0	0.0	0.0	1.848517673677478E-4	3.2349059289355864E-4
16	0.0	0.0	0.0	1.848517673677478E-4	4.621294184193695E-4
17	0.0	0.0	0.0	1.848517673677478E-4	4.621294184193695E-4
18	0.0	0.0	0.0	1.848517673677478E-4	5.545553021032434E-4
19	0.0	0.0	0.0	2.3106470920968475E-4	6.007682439451804E-4
20	0.0	0.0	0.0	3.697035347354956E-4	6.007682439451804E-4
21	0.0	0.0	0.0	7.856200113129281E-4	6.007682439451804E-4
22	0.0	0.0	0.0	0.0010628976623645499	6.007682439451804E-4
23	0.0	0.0	0.0	0.0015250270807839195	6.469811857871173E-4
24	0.0	0.0	0.0	0.0024492859176226582	6.469811857871173E-4
25	0.0	0.0	0.0	0.0026341376849904063	6.469811857871173E-4
26	0.0	0.0	0.0	0.0032811188707775234	6.469811857871173E-4
27	0.0	0.0	0.0	0.008087264822338966	7.394070694709912E-4
28	0.0	0.0	0.0	0.018392750853090906	7.394070694709912E-4
29	0.0	0.0	0.0	0.031147522801465505	7.394070694709912E-4
30	0.0	0.0	0.0	0.05337594782743718	7.394070694709912E-4
31	0.0	0.0	0.0	0.13203037484241387	7.394070694709912E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGT	45	0.0038268827	20.555557	7
CGAACGT	180	0.0	19.527779	4
TAGACTA	180	0.0	18.5	5
ACGAACG	255	0.0	18.137255	15
ATACCGT	485	0.0	17.927835	6
TACCCCG	110	7.8154335E-7	16.818182	5
TAAGCGT	80	3.384292E-4	16.1875	4
TAGAGTG	505	0.0	16.11881	5
TAACGAA	300	0.0	16.033333	13
GTAATAC	220	0.0	15.977273	3
TCGAACG	220	0.0	15.977273	3
TAGGTCG	70	0.0025934847	15.857143	5
TACCGTG	140	3.477544E-8	15.857143	7
ACGCGTA	70	0.0025934847	15.857143	6
TTAGAGT	430	0.0	15.488372	4
CGAACGA	300	0.0	15.416667	16
GCGCAAG	600	0.0	15.416667	1
CGTCGTA	465	0.0	15.118279	10
ACCGTCG	490	0.0	14.724489	8
AAGACGG	605	0.0	14.677686	5
>>END_MODULE