FastQCFastQC Report
Thu 9 Feb 2017
SRR2727157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5613804
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210500.3749685596433363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154560.27532133291436606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121390.21623483826653014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78570.13995857354478353No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT62860.11197398412912171No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC56420.10050226192435646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15600.016.2467965
CAAGACG17300.016.0404624
CGAACGA7700.015.37662516
TATACTG8550.015.3625735
ACGAACG7850.015.08280315
CGCAAGA18550.014.9595692
ATACCGT13450.014.8550196
GTATTAG12950.014.4285711
TCGTTAC9900.014.38888923
TACCGTC12800.014.16406257
CGAACGT4200.014.0952384
ACGGATG20750.014.08674728
TTAGAGT9750.014.0410254
ACGGACC17800.013.9269668
TACGGAT20600.013.91990227
TATGCGA1203.304851E-513.87499912
GTTACGG15650.013.712460525
CGTCGTA12550.013.70916410
GCGCAAG19600.013.5918361
ACCGTCG12800.013.58593758
Produced by FastQC (version 0.11.5)